{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:37:04Z","timestamp":1789879024934,"version":"4.0.1"},"reference-count":0,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[1989,10]]},"abstract":"<jats:p>The group I self-splicing introns act at exon-intron junctions without recognizing a particular sequence. In order to understand splice-site selection, we have developed an assay system based on the Tetrahymena ribozyme to allow the study of numerous 5'-splice-site variants. Cleavage at the correct site requires formation of the correct secondary structure and occurs most efficiently within a 3-base-pair window centered on base pair 5 from the bottom of the P1 stem. Within this window the ribozyme recognizes and cleaves at a \"wobble\" base pair; the base pair above the cleavage site also influences splicing efficiency. The recognition of RNA structure rather than sequence explains the ability of these transposable introns to splice out of a variety of sequence contexts.<\/jats:p>","DOI":"10.1073\/pnas.86.19.7402","type":"journal-article","created":{"date-parts":[[2006,5,31]],"date-time":"2006-05-31T07:03:53Z","timestamp":1149059033000},"page":"7402-7406","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":70,"title":["RNA structure, not sequence, determines the 5' splice-site specificity of a group I intron."],"prefix":"10.1073","volume":"86","author":[{"given":"J A","family":"Doudna","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Massachusetts General Hospital, Boston 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B P","family":"Cormack","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Massachusetts General Hospital, Boston 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J W","family":"Szostak","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Massachusetts General Hospital, Boston 02114."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[1989,10]]},"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.86.19.7402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T12:41:05Z","timestamp":1649853665000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.86.19.7402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10]]},"references-count":0,"journal-issue":{"issue":"19","published-print":{"date-parts":[[1989,10]]}},"alternative-id":["10.1073\/pnas.86.19.7402"],"URL":"https:\/\/doi.org\/10.1073\/pnas.86.19.7402","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10]]},"assertion":[{"value":"1989-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}