{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T16:18:53Z","timestamp":1772813933478,"version":"3.50.1"},"reference-count":23,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2003,11,1]],"date-time":"2003-11-01T00:00:00Z","timestamp":1067644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2003,11,1]],"date-time":"2003-11-01T00:00:00Z","timestamp":1067644800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T00:00:00Z","timestamp":1601337600000},"content-version":"vor","delay-in-days":6177,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["mcponline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular &amp; Cellular Proteomics"],"published-print":{"date-parts":[[2003,11]]},"DOI":"10.1074\/mcp.t300007-mcp200","type":"journal-article","created":{"date-parts":[[2003,9,8]],"date-time":"2003-09-08T22:31:26Z","timestamp":1063060286000},"page":"1225-1233","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":99,"title":["Identification of Novel Protein-Protein Interactions Using A Versatile Mammalian Tandem Affinity Purification Expression System"],"prefix":"10.1016","volume":"2","author":[{"given":"Matthew","family":"Knuesel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhan","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Holinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nick","family":"Lowe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuedong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/mcp.T300007-MCP200_bib1","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0092-8674(00)80922-8","article-title":"The cell as a collection of protein machines: preparing the next generation of molecular biologists","volume":"92","author":"Alberts","year":"1998","journal-title":"Cell"},{"key":"10.1074\/mcp.T300007-MCP200_bib3","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1038\/13732","article-title":"A generic protein purification method for protein complex characterization and proteome exploration","volume":"17","author":"Rigaut","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"10.1074\/mcp.T300007-MCP200_bib4","doi-asserted-by":"crossref","first-page":"E24","DOI":"10.1093\/nar\/29.4.e24","article-title":"A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex","volume":"29","author":"Honey","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.T300007-MCP200_bib5","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/415141a","article-title":"Functional organization of the yeast proteome by systematic analysis of protein complexes","volume":"415","author":"Gavin","year":"2002","journal-title":"Nature"},{"key":"10.1074\/mcp.T300007-MCP200_bib6","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/415180a","article-title":"Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry","volume":"415","author":"Ho","year":"2002","journal-title":"Nature"},{"key":"10.1074\/mcp.T300007-MCP200_bib7","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1046\/j.1432-1033.2003.03428.x","article-title":"Affinity purification-mass spectrometry. Powerful tools for the characterization of protein complexes","volume":"270","author":"Bauer","year":"2003","journal-title":"Eur. J. Biochem."},{"key":"10.1074\/mcp.T300007-MCP200_bib8","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1006\/abio.2000.4478","article-title":"Generation of mammalian cells stably expressing multiple genes at predetermined levels","volume":"280","author":"Liu","year":"2000","journal-title":"Anal. Biochem."},{"key":"10.1074\/mcp.T300007-MCP200_bib9","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1002\/1522-2683(200105)22:9<1677::AID-ELPS1677>3.0.CO;2-H","volume":"22","author":"Castellanos-Serra","year":"2001","journal-title":"Electrophoresis"},{"key":"10.1074\/mcp.T300007-MCP200_bib10","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/S0065-230X(08)60765-4","article-title":"Signal transduction through MAP kinase cascades","volume":"74","author":"Lewis","year":"1998","journal-title":"Adv. Cancer Res."},{"key":"10.1074\/mcp.T300007-MCP200_bib11","first-page":"218","article-title":"The tandem affinity purification (TAP) method: a general procedure of protein complex purification","volume":"24","author":"Puig","year":"2001","journal-title":"Methods (Orlando)"},{"key":"10.1074\/mcp.T300007-MCP200_bib12","doi-asserted-by":"crossref","first-page":"8103","DOI":"10.1128\/.20.21.8103-8111.2000","article-title":"Inactivation of smad-transforming growth factor beta signaling by Ca(2+)-calmodulin-dependent protein kinase II","volume":"20","author":"Wicks","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"10.1074\/mcp.T300007-MCP200_bib13","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1074\/jbc.273.2.677","article-title":"Smad proteins physically interact with calmodulin","volume":"273","author":"Zimmerman","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.T300007-MCP200_bib14","doi-asserted-by":"crossref","first-page":"41430","DOI":"10.1074\/jbc.M005727200","article-title":"Calmodulin differentially modulates Smad1 and Smad2 signaling","volume":"275","author":"Scherer","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.T300007-MCP200_bib15","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/S0092-8674(03)00432-X","article-title":"Mechanisms of TGF-beta signaling from cell membrane to the nucleus","volume":"113","author":"Shi","year":"2003","journal-title":"Cell"},{"key":"10.1074\/mcp.T300007-MCP200_bib16","doi-asserted-by":"crossref","first-page":"10669","DOI":"10.1073\/pnas.94.20.10669","article-title":"Transforming growth factor beta-induced phosphorylation of Smad3 is required for growth inhibition and transcriptional induction in epithelial cells","volume":"94","author":"Liu","year":"1997","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1074\/mcp.T300007-MCP200_bib17","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1101\/gad.12.19.3084","article-title":"Synergistic cooperation of TFE3 and smad proteins in TGF-beta-induced transcription of the plasminogen activator inhibitor-1 gene","volume":"12","author":"Hua","year":"1998","journal-title":"Genes Dev."},{"key":"10.1074\/mcp.T300007-MCP200_bib18","doi-asserted-by":"crossref","first-page":"7853","DOI":"10.1073\/pnas.97.14.7853","article-title":"A distinct nuclear localization signal in the N terminus of Smad 3 determines its ligand-induced nuclear translocation","volume":"97","author":"Xiao","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1074\/mcp.T300007-MCP200_bib19","doi-asserted-by":"crossref","first-page":"23425","DOI":"10.1074\/jbc.C000345200","article-title":"Importin beta mediates nuclear translocation of Smad 3","volume":"275","author":"Xiao","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.T300007-MCP200_bib20","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0092-8674(00)80928-9","article-title":"The Hsp70 and Hsp60 chaperone machines","volume":"92","author":"Bukau","year":"1998","journal-title":"Cell"},{"key":"10.1074\/mcp.T300007-MCP200_bib21","first-page":"306","article-title":"Steroid receptor interactions with heat shock protein and immunophilin chaperones","volume":"18","author":"Pratt","year":"1997","journal-title":"Endocr. Rev."},{"key":"10.1074\/mcp.T300007-MCP200_bib22","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0092-8674(00)80806-5","article-title":"Getting newly synthesized proteins into shape","volume":"101","author":"Bukau","year":"2000","journal-title":"Cell"},{"key":"10.1074\/mcp.T300007-MCP200_bib23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nbt773","article-title":"An efficient protein complex purification method for functional proteomics in higher eukaryotes","volume":"21","author":"Forler","year":"2003","journal-title":"Nat. Biotechnol."},{"key":"10.1074\/mcp.T300007-MCP200_bib2","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1074\/mcp.M200027-MCP200","article-title":"The identification of protein-protein interactions of the nuclear pore complex of Saccharomyces cerevisiae using high throughput matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry","volume":"1","author":"Huang","year":"2002","journal-title":"Mol. Cell. Proteomics"}],"container-title":["Molecular &amp; Cellular Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620345801?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620345801?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/mcp.T300007-MCP200","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T01:02:27Z","timestamp":1761526947000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1535947620345801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11]]},"references-count":23,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2003,11]]}},"alternative-id":["S1535947620345801"],"URL":"https:\/\/doi.org\/10.1074\/mcp.t300007-mcp200","relation":{},"ISSN":["1535-9476"],"issn-type":[{"value":"1535-9476","type":"print"}],"subject":[],"published":{"date-parts":[[2003,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of Novel Protein-Protein Interactions Using A Versatile Mammalian Tandem Affinity Purification Expression System","name":"articletitle","label":"Article Title"},{"value":"Molecular & Cellular Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/mcp.T300007-MCP200","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2003 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}