{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:06:05Z","timestamp":1790363165785,"version":"4.1.0"},"reference-count":41,"publisher":"Elsevier BV","issue":"46","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T00:00:00Z","timestamp":1601424000000},"content-version":"vor","delay-in-days":8004,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[1998,11]]},"DOI":"10.1074\/jbc.273.46.30583","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T15:02:22Z","timestamp":1027695742000},"page":"30583-30590","source":"Crossref","is-referenced-by-count":125,"title":["Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius"],"prefix":"10.1016","volume":"273","author":[{"given":"Jesus G.","family":"Valenzuela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosane","family":"Charlab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Y.","family":"Galperin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.C.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.273.46.30583_bib1","first-page":"143","volume":"4","author":"Ribeiro","year":"1995","journal-title":"Infect. Agents Dis."},{"key":"10.1074\/jbc.273.46.30583_bib2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1146\/annurev.bi.61.070192.000511","volume":"61","author":"Law","year":"1992","journal-title":"Annu. Rev. Biochem."},{"key":"10.1074\/jbc.273.46.30583_bib3","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1096\/fasebj.7.6.8472890","volume":"7","author":"Marcus","year":"1993","journal-title":"FASEB J."},{"key":"10.1074\/jbc.273.46.30583_bib4","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1146\/annurev.en.32.010187.002335","volume":"32","author":"Ribeiro","year":"1987","journal-title":"Annu. Rev. Entomol."},{"key":"10.1074\/jbc.273.46.30583_bib5","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1073\/pnas.92.3.694","volume":"92","author":"Champagne","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1074\/jbc.273.46.30583_bib6","series-title":"The Immunology of Host-Ectoparasitic Arthropod Relationships","first-page":"85","author":"Champagne","year":"1996"},{"key":"10.1074\/jbc.273.46.30583_bib7","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0074-7696(08)62487-0","volume":"158","author":"Plesner","year":"1995","journal-title":"Int. Rev. Cytol."},{"key":"10.1074\/jbc.273.46.30583_bib8","doi-asserted-by":"crossref","first-page":"11391","DOI":"10.1074\/jbc.270.19.11391","volume":"270","author":"Asai","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib9","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1006\/bbrc.1996.0162","volume":"218","author":"Handa","year":"1996","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1074\/jbc.273.46.30583_bib10","doi-asserted-by":"crossref","first-page":"16323","DOI":"10.1074\/jbc.271.27.16323","volume":"271","author":"Strobel","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib11","doi-asserted-by":"crossref","first-page":"16043","DOI":"10.1074\/jbc.273.26.16043","volume":"273","author":"Nagy","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib12","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/S0169-328X(97)00066-1","volume":"47","author":"Wang","year":"1997","journal-title":"Mol. Brain Res."},{"key":"10.1074\/jbc.273.46.30583_bib13","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/S0028-3908(97)00115-9","volume":"36","author":"Kegel","year":"1997","journal-title":"Neuropharmacology"},{"key":"10.1074\/jbc.273.46.30583_bib14","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1432-1033.1995.066_c.x","volume":"234","author":"Christoforidis","year":"1995","journal-title":"Eur. J. Biochem."},{"key":"10.1074\/jbc.273.46.30583_bib15","doi-asserted-by":"crossref","first-page":"33116","DOI":"10.1074\/jbc.271.51.33116","volume":"271","author":"Kaczmarek","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib16","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1074\/jbc.272.2.1076","volume":"272","author":"Kirley","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib17","doi-asserted-by":"crossref","first-page":"22139","DOI":"10.1074\/jbc.271.36.22139","volume":"271","author":"Vasconcelos","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib18","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0304-4165(96)00079-7","volume":"1334","author":"Sevigny","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.273.46.30583_bib19","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1083\/jcb.122.2.307","volume":"122","author":"Abeijon","year":"1993","journal-title":"J. Cell Biol."},{"key":"10.1074\/jbc.273.46.30583_bib20","doi-asserted-by":"crossref","first-page":"9898","DOI":"10.1074\/jbc.271.17.9898","volume":"271","author":"Wang","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib21","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0965-1748(96)00041-0","volume":"26","author":"Valenzuela","year":"1996","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1074\/jbc.273.46.30583_bib22","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/0049-3848(95)93881-Y","volume":"77","author":"Bednar","year":"1995","journal-title":"Thromb. Res."},{"key":"10.1074\/jbc.273.46.30583_bib23","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1113\/jphysiol.1963.sp007185","volume":"168","author":"Born","year":"1963","journal-title":"J. Physiol. (Lond.)"},{"key":"10.1074\/jbc.273.46.30583_bib24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/protein\/10.1.1","volume":"10","author":"Nielsen","year":"1997","journal-title":"Protein Eng."},{"key":"10.1074\/jbc.273.46.30583_bib25","first-page":"109","volume":"4","author":"Horton","year":"1996","journal-title":"Intelligent Systems Mol. Biol."},{"key":"10.1074\/jbc.273.46.30583_bib26","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/jbc.273.46.30583_bib27","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1002\/prot.340090304","volume":"9","author":"Schuler","year":"1991","journal-title":"Proteins"},{"key":"10.1074\/jbc.273.46.30583_bib28","first-page":"155","volume":"8","author":"Huang","year":"1992","journal-title":"Comput. Appl. Biosci."},{"key":"10.1074\/jbc.273.46.30583_bib29","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1093\/nar\/25.1.217","volume":"25","author":"Bairoch","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/jbc.273.46.30583_bib30","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1093\/nar\/26.1.320","volume":"26","author":"Sonnhammer","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/jbc.273.46.30583_bib31","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1002\/pro.5560061218","volume":"6","author":"Galperin","year":"1997","journal-title":"Protein Sci."},{"key":"10.1074\/jbc.273.46.30583_bib32","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0968-0004(98)01189-X","volume":"23","author":"Aravind","year":"1998","journal-title":"Trends Biochem. Sci."},{"key":"10.1074\/jbc.273.46.30583_bib33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1042\/bj2900205","volume":"290","author":"Rawlings","year":"1993","journal-title":"Biochem. J."},{"key":"10.1074\/jbc.273.46.30583_bib34","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/S0969-2126(01)00220-9","volume":"3","author":"Davies","year":"1995","journal-title":"Structure"},{"key":"10.1074\/jbc.273.46.30583_bib35","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0968-0004(94)90167-8","volume":"19","author":"Doolittle","year":"1994","journal-title":"Trends Biochem. Sci."},{"key":"10.1074\/jbc.273.46.30583_bib36","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1101\/gr.8.8.779","volume":"8","author":"Galperin","year":"1998","journal-title":"Genome Res."},{"key":"10.1074\/jbc.273.46.30583_bib37","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1002\/pro.5560070819","volume":"7","author":"Galperin","year":"1998","journal-title":"Protein Sci."},{"key":"10.1074\/jbc.273.46.30583_bib38","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1042\/bj2330885","volume":"233","author":"Sarkis","year":"1986","journal-title":"Biochem. J."},{"key":"10.1074\/jbc.273.46.30583_bib39","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1006\/bbrc.1998.8714","volume":"246","author":"Woodhouse","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1074\/jbc.273.46.30583_bib40","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1074\/jbc.272.2.996","volume":"272","author":"Clair","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.273.46.30583_bib41","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1016\/S0076-6879(96)66033-9","volume":"266","author":"Rost","year":"1996","journal-title":"Methods Enzymol."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925819592754?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925819592754?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.273.46.30583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T21:06:19Z","timestamp":1618434379000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925819592754"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,11]]},"references-count":41,"journal-issue":{"issue":"46","published-print":{"date-parts":[[1998,11]]}},"alternative-id":["S0021925819592754"],"URL":"https:\/\/doi.org\/10.1074\/jbc.273.46.30583","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[1998,11]]}}}