{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:28:48Z","timestamp":1791412128993,"version":"4.3.3"},"reference-count":27,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T00:00:00Z","timestamp":1598486400000},"content-version":"vor","delay-in-days":1700,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["647858 - GENESIS"],"award-info":[{"award-number":["647858 - GENESIS"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC1023"],"award-info":[{"award-number":["EXC1023"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2016,1]]},"DOI":"10.1074\/jbc.c115.700492","type":"journal-article","created":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T22:39:15Z","timestamp":1447108755000},"page":"103-109","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":454,"title":["A Genome-wide CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Screen Identifies NEK7 as an Essential Component of NLRP3 Inflammasome Activation"],"prefix":"10.1016","volume":"291","author":[{"given":"Jonathan L.","family":"Schmid-Burgk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhruv","family":"Chauhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Ebert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Reinhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmar","family":"Endl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veit","family":"Hornung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.C115.700492_bib1","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1038\/nature06246","article-title":"Recognition of microorganisms and activation of the immune response","volume":"449","author":"Medzhitov","year":"2007","journal-title":"Nature"},{"key":"10.1074\/jbc.C115.700492_bib2","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1038\/nri2873","article-title":"Sterile inflammation: sensing and reacting to damage","volume":"10","author":"Chen","year":"2010","journal-title":"Nat. Rev. Immunol"},{"key":"10.1074\/jbc.C115.700492_bib3","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1600-065X.2011.01046.x","article-title":"The inflammasome: an integrated view","volume":"243","author":"Gross","year":"2011","journal-title":"Immunol. Rev"},{"key":"10.1074\/jbc.C115.700492_bib4","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/nature15541","article-title":"Caspase-11 cleaves gasdermin D for non-canonical inflammasome signaling","volume":"526","author":"Kayagaki","year":"2015","journal-title":"Nature"},{"key":"10.1074\/jbc.C115.700492_bib5","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/nature15514","article-title":"Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death","volume":"526","author":"Shi","year":"2015","journal-title":"Nature"},{"key":"10.1074\/jbc.C115.700492_bib6","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/nri3452","article-title":"Activation and regulation of the inflammasomes","volume":"13","author":"Latz","year":"2013","journal-title":"Nat. Rev. Immunol"},{"key":"10.1074\/jbc.C115.700492_bib7","doi-asserted-by":"crossref","first-page":"81ps17","DOI":"10.1126\/scitranslmed.3001902","article-title":"The inflammasome in atherosclerosis and type 2 diabetes","volume":"3","author":"Masters","year":"2011","journal-title":"Sci. Transl. Med"},{"key":"10.1074\/jbc.C115.700492_bib8","doi-asserted-by":"crossref","first-page":"787","DOI":"10.4049\/jimmunol.0901363","article-title":"Cutting edge: NF-\u03baB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression","volume":"183","author":"Bauernfeind","year":"2009","journal-title":"J. Immunol"},{"key":"10.1074\/jbc.C115.700492_bib9","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1038\/ni.2913","article-title":"The adaptor ASC has extracellular and \u2018prionoid\u2019 activities that propagate inflammation","volume":"15","author":"Franklin","year":"2014","journal-title":"Nat. Immunol"},{"key":"10.1074\/jbc.C115.700492_bib10","doi-asserted-by":"crossref","first-page":"36617","DOI":"10.1074\/jbc.M112.407130","article-title":"Non-transcriptional priming and deubiquitination regulate NLRP3 inflammasome activation","volume":"287","author":"Juliana","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.C115.700492_bib11","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.imbio.2012.07.020","article-title":"Acute lipopolysaccharide priming boosts inflammasome activation independently of inflammasome sensor induction","volume":"217","author":"Schroder","year":"2012","journal-title":"Immunobiology"},{"key":"10.1074\/jbc.C115.700492_bib12","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1038\/sj.cdd.4402195","article-title":"Activation of the NALP3 inflammasome is triggered by low intracellular potassium concentration","volume":"14","author":"P\u00e9trilli","year":"2007","journal-title":"Cell Death Differ"},{"key":"10.1074\/jbc.C115.700492_bib13","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1016\/j.immuni.2013.05.016","article-title":"K+ efflux is the common trigger of NLRP3 inflammasome activation by bacterial toxins and particulate matter","volume":"38","author":"Mu\u00f1oz-Planillo","year":"2013","journal-title":"Immunity"},{"key":"10.1074\/jbc.C115.700492_bib14","doi-asserted-by":"crossref","first-page":"14987","DOI":"10.1038\/srep14987","article-title":"Synthesis of an arrayed sgRNA library targeting the human genome","volume":"5","author":"Schmidt","year":"2015","journal-title":"Sci. Rep"},{"key":"10.1074\/jbc.C115.700492_bib15","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1101\/gr.176701.114","article-title":"OutKnocker: a web tool for rapid and simple genotyping of designer nuclease edited cell lines","volume":"24","author":"Schmid-Burgk","year":"2014","journal-title":"Genome Res"},{"key":"10.1074\/jbc.C115.700492_bib16","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/978-1-4939-2932-0_19","article-title":"Designer nuclease-mediated generation of knockout THP1 cells","volume":"1338","author":"Schmidt","year":"2016","journal-title":"Methods Mol. Biol"},{"key":"10.1074\/jbc.C115.700492_bib17","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-62703-523-1_9","article-title":"Immunoblotting for active caspase-1","volume":"1040","author":"Jakobs","year":"2013","journal-title":"Methods Mol. Biol"},{"key":"10.1074\/jbc.C115.700492_bib18","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1126\/science.1247005","article-title":"Genome-scale CRISPR-Cas9 knockout screening in human cells","volume":"343","author":"Shalem","year":"2014","journal-title":"Science"},{"key":"10.1074\/jbc.C115.700492_bib19","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1126\/science.1246981","article-title":"Genetic screens in human cells using the CRISPR-Cas9 system","volume":"343","author":"Wang","year":"2014","journal-title":"Science"},{"key":"10.1074\/jbc.C115.700492_bib20","doi-asserted-by":"crossref","first-page":"R130","DOI":"10.1186\/gb-2009-10-11-r130","article-title":"BioGPS: an extensible and customizable portal for querying and organizing gene annotation resources","volume":"10","author":"Wu","year":"2009","journal-title":"Genome Biol"},{"key":"10.1074\/jbc.C115.700492_bib21","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/ni.2550","article-title":"Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome","volume":"14","author":"Misawa","year":"2013","journal-title":"Nat. Immunol"},{"key":"10.1074\/jbc.C115.700492_bib22","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/j.bbamcr.2012.12.021","article-title":"Nek7 kinase accelerates microtubule dynamic instability","volume":"1833","author":"Cohen","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.C115.700492_bib23","doi-asserted-by":"crossref","first-page":"3975","DOI":"10.1128\/MCB.01867-08","article-title":"The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis","volume":"29","author":"O'Regan","year":"2009","journal-title":"Mol. Cell. Biol"},{"key":"10.1074\/jbc.C115.700492_bib24","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1242\/jcs.048975","article-title":"The NIMA-family kinase Nek3 regulates microtubule acetylation in neurons","volume":"122","author":"Chang","year":"2009","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.C115.700492_bib25","doi-asserted-by":"crossref","first-page":"4046","DOI":"10.1038\/onc.2010.162","article-title":"Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy","volume":"29","author":"Salem","year":"2010","journal-title":"Oncogene"},{"key":"10.1074\/jbc.C115.700492_bib26","doi-asserted-by":"crossref","first-page":"5161","DOI":"10.1242\/jcs.02681","article-title":"Caught Nek-ing: cilia and centrioles","volume":"118","author":"Quarmby","year":"2005","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.C115.700492_bib27","first-page":"4423","article-title":"Cell cycle regulation by the NEK family of protein kinases","volume":"125","author":"Fry","year":"2012","journal-title":"J. Cell Sci"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820362426?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820362426?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.C115.700492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T02:50:08Z","timestamp":1641264608000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820362426"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["S0021925820362426"],"URL":"https:\/\/doi.org\/10.1074\/jbc.c115.700492","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725926654.793513701","asserted-by":"object"}]},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2016,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A Genome-wide CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Screen Identifies NEK7 as an Essential Component of NLRP3 Inflammasome Activation","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.C115.700492","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}