{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:56:46Z","timestamp":1791327406060,"version":"4.3.1"},"reference-count":65,"publisher":"Elsevier BV","issue":"52","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,29]],"date-time":"2020-08-29T00:00:00Z","timestamp":1598659200000},"content-version":"vor","delay-in-days":3924,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2009,12]]},"DOI":"10.1074\/jbc.m109.028795","type":"journal-article","created":{"date-parts":[[2009,9,29]],"date-time":"2009-09-29T00:49:19Z","timestamp":1254185359000},"page":"36462-36472","source":"Crossref","is-referenced-by-count":67,"title":["Overexpression, Isolation, and Spectroscopic Characterization of the Bidirectional [NiFe] Hydrogenase from Synechocystis sp. PCC 6803"],"prefix":"10.1016","volume":"284","author":[{"given":"Frauke","family":"Germer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingo","family":"Zebger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Saggu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedhelm","family":"Lendzian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00fcdiger","family":"Schulz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Appel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M109.028795_bib1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/S0168-6445(01)00063-8","volume":"25","author":"Vignais","year":"2001","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1074\/jbc.M109.028795_bib2","doi-asserted-by":"crossref","first-page":"4206","DOI":"10.1021\/cr050196r","volume":"107","author":"Vignais","year":"2007","journal-title":"Chem. Rev."},{"key":"10.1074\/jbc.M109.028795_bib3","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1126\/science.1161326","volume":"321","author":"Armstrong","year":"2008","journal-title":"Science"},{"key":"10.1074\/jbc.M109.028795_bib4","doi-asserted-by":"crossref","first-page":"4304","DOI":"10.1021\/cr0501947","volume":"107","author":"De Lacey","year":"2007","journal-title":"Chem. Rev."},{"key":"10.1074\/jbc.M109.028795_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1011-1344(98)00179-1","volume":"47","author":"Appel","year":"1998","journal-title":"J. Photochem. Photobiol."},{"key":"10.1074\/jbc.M109.028795_bib6","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1111\/j.1574-6976.2007.00085.x","volume":"31","author":"Tamagnini","year":"2007","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1074\/jbc.M109.028795_bib7","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1111\/j.1432-1033.1995.266_1.x","volume":"233","author":"Schmitz","year":"1995","journal-title":"Eur. J. Biochem."},{"key":"10.1074\/jbc.M109.028795_bib8","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/S0167-4838(96)00176-8","volume":"1298","author":"Appel","year":"1996","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib9","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/S0005-2728(02)00214-1","volume":"1554","author":"Schmitz","year":"2002","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib10","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s002030000139","volume":"173","author":"Appel","year":"2000","journal-title":"Arch. Microbiol."},{"key":"10.1074\/jbc.M109.028795_bib11","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1016\/S0360-3199(02)00105-2","volume":"27","author":"Cournac","year":"2002","journal-title":"Int. J. Hydrogen Energy"},{"key":"10.1074\/jbc.M109.028795_bib12","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1128\/JB.186.6.1737-1746.2003","volume":"186","author":"Cournac","year":"2004","journal-title":"J. Bacteriol."},{"key":"10.1074\/jbc.M109.028795_bib13","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.bbabio.2006.12.003","volume":"1767","author":"Gutthann","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib14","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1111\/j.1365-2958.2005.04867.x","volume":"58","author":"Gutekunst","year":"2005","journal-title":"Mol. Microbiol."},{"key":"10.1074\/jbc.M109.028795_bib15","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0014-5793(99)01799-8","volume":"466","author":"Happe","year":"2000","journal-title":"FEBS Lett."},{"key":"10.1074\/jbc.M109.028795_bib16","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1007\/s00775-004-0555-y","volume":"9","author":"Van der Linden","year":"2004","journal-title":"J. Biol. Inorg. Chem."},{"key":"10.1074\/jbc.M109.028795_bib17","doi-asserted-by":"crossref","first-page":"23791","DOI":"10.1074\/jbc.M503260200","volume":"280","author":"Buhrke","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M109.028795_bib18","doi-asserted-by":"crossref","first-page":"16264","DOI":"10.1074\/jbc.M805690200","volume":"284","author":"Saggu","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M109.028795_bib19","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0167-4838(85)90175-X","volume":"832","author":"Fernandez","year":"1985","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib20","doi-asserted-by":"crossref","first-page":"6820","DOI":"10.1021\/bi049854c","volume":"43","author":"Kurkin","year":"2004","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M109.028795_bib21","doi-asserted-by":"crossref","first-page":"9706","DOI":"10.1021\/bi0602462","volume":"45","author":"Fichtner","year":"2006","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M109.028795_bib22","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/373580a0","volume":"373","author":"Volbeda","year":"1995","journal-title":"Nature"},{"key":"10.1074\/jbc.M109.028795_bib23","doi-asserted-by":"crossref","first-page":"12989","DOI":"10.1021\/ja962270g","volume":"118","author":"Volbeda","year":"1996","journal-title":"J. Am. Chem. Soc."},{"key":"10.1074\/jbc.M109.028795_bib24","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/0167-4838(92)90386-R","volume":"1119","author":"Coremans","year":"1992","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib25","doi-asserted-by":"crossref","first-page":"4980","DOI":"10.1021\/bi00182a029","volume":"33","author":"Surerus","year":"1994","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M109.028795_bib26","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1007\/s00775-004-0570-z","volume":"9","author":"Bleijlevens","year":"2004","journal-title":"J. Biol. Inorg. Chem."},{"key":"10.1074\/jbc.M109.028795_bib27","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/0167-4838(92)90126-X","volume":"1122","author":"Asso","year":"1992","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib28","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1016\/S0969-2126(97)00313-4","volume":"5","author":"Higuchi","year":"1997","journal-title":"Structure"},{"key":"10.1074\/jbc.M109.028795_bib29","doi-asserted-by":"crossref","first-page":"4331","DOI":"10.1021\/cr050186q","volume":"107","author":"Lubitz","year":"2007","journal-title":"Chem. Rev."},{"key":"10.1074\/jbc.M109.028795_bib30","doi-asserted-by":"crossref","first-page":"11628","DOI":"10.1021\/ja012645k","volume":"124","author":"Ogata","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"10.1074\/jbc.M109.028795_bib31","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/385126a0","volume":"385","author":"Happe","year":"1997","journal-title":"Nature"},{"key":"10.1074\/jbc.M109.028795_bib32","doi-asserted-by":"crossref","first-page":"7181","DOI":"10.1021\/ja963802w","volume":"119","author":"De Lacey","year":"1997","journal-title":"J. Am. Chem. Soc."},{"key":"10.1074\/jbc.M109.028795_bib33","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1007\/s00775-001-0301-7","volume":"7","author":"De Lacey","year":"2002","journal-title":"J. Biol. Inorg. Chem."},{"key":"10.1074\/jbc.M109.028795_bib34","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.str.2005.07.018","volume":"13","author":"Ogata","year":"2005","journal-title":"Structure"},{"key":"10.1074\/jbc.M109.028795_bib35","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s00775-005-0632-x","volume":"10","author":"Volbeda","year":"2005","journal-title":"J. Biol. Inorg. Chem."},{"key":"10.1074\/jbc.M109.028795_bib36","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/S0969-2126(99)80072-0","volume":"7","author":"Garcin","year":"1999","journal-title":"Structure"},{"key":"10.1074\/jbc.M109.028795_bib37","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/S0969-2126(99)80071-9","volume":"7","author":"Higuchi","year":"1999","journal-title":"Structure"},{"key":"10.1074\/jbc.M109.028795_bib38","doi-asserted-by":"crossref","first-page":"9291","DOI":"10.1021\/ja9700919","volume":"119","author":"Huyett","year":"1997","journal-title":"J. Am. Chem. Soc."},{"key":"10.1074\/jbc.M109.028795_bib39","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s00775-005-0075-4","volume":"11","author":"van der Linden","year":"2006","journal-title":"J. Biol. Inorg. Chem."},{"key":"10.1074\/jbc.M109.028795_bib40","first-page":"1","volume":"111","author":"Rippka","year":"1979","journal-title":"J. Gen. Microbiol."},{"key":"10.1074\/jbc.M109.028795_bib41","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"2001"},{"key":"10.1074\/jbc.M109.028795_bib42","doi-asserted-by":"crossref","first-page":"4516","DOI":"10.1111\/j.1742-4658.2006.05460.x","volume":"273","author":"Hoffmann","year":"2006","journal-title":"FEBS J."},{"key":"10.1074\/jbc.M109.028795_bib43","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1104\/pp.48.1.108","volume":"48","author":"Wang","year":"1971","journal-title":"Plant Physiol."},{"key":"10.1074\/jbc.M109.028795_bib44","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1074\/jbc.M109.028795_bib45","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1038\/nprot.2006.4","volume":"1","author":"Sch\u00e4gger","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1074\/jbc.M109.028795_bib46","doi-asserted-by":"crossref","first-page":"13467","DOI":"10.1021\/bi048837k","volume":"43","author":"Jones","year":"2004","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M109.028795_bib47","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1063\/1.1140633","volume":"60","author":"Stesmans","year":"1989","journal-title":"Rev. Sci. Instrum."},{"key":"10.1074\/jbc.M109.028795_bib48","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.jmr.2005.08.013","volume":"178","author":"Stoll","year":"2006","journal-title":"J. Magn. Reson."},{"key":"10.1074\/jbc.M109.028795_bib49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0065-2911(06)51001-X","volume":"51","author":"B\u00f6ck","year":"2006","journal-title":"Adv. Microb. Physiol."},{"key":"10.1074\/jbc.M109.028795_bib50","doi-asserted-by":"crossref","first-page":"17439","DOI":"10.1074\/jbc.273.28.17439","volume":"273","author":"M\u00e1t\u00e9","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M109.028795_bib51","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S0005-2728(98)00027-9","volume":"1364","author":"Ohnishi","year":"1998","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib52","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0005-2795(73)90072-X","volume":"295","author":"Mukai","year":"1973","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M109.028795_bib53","doi-asserted-by":"crossref","first-page":"4781","DOI":"10.1021\/bi00014a036","volume":"34","author":"Guigliarelli","year":"1995","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M109.028795_bib54","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/S0898-8838(08)60084-7","volume":"47","author":"Guigliarelli","year":"1999","journal-title":"Adv. Inorg. Chem."},{"key":"10.1074\/jbc.M109.028795_bib55","doi-asserted-by":"crossref","first-page":"16602","DOI":"10.1074\/jbc.M709894200","volume":"283","author":"Schumann","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M109.028795_bib56","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0014-5793(96)00228-1","volume":"383","author":"Serebryakova","year":"1996","journal-title":"FEBS Lett."},{"key":"10.1074\/jbc.M109.028795_bib57","doi-asserted-by":"crossref","first-page":"5070","DOI":"10.1016\/S0021-9258(18)83701-2","volume":"264","author":"Bryant","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M109.028795_bib58","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/j.1432-1033.1984.tb08252.x","volume":"142","author":"Schneider","year":"1984","journal-title":"Eur. J. Biochem."},{"key":"10.1074\/jbc.M109.028795_bib59","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1007\/s00775-006-0162-1","volume":"12","author":"Long","year":"2007","journal-title":"J. Biol. Inorg. Chem."},{"key":"10.1074\/jbc.M109.028795_bib60","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1046\/j.1365-2958.2001.02612.x","volume":"41","author":"Schmitz","year":"2001","journal-title":"Mol. Microbiol."},{"key":"10.1074\/jbc.M109.028795_bib61","doi-asserted-by":"crossref","first-page":"3122","DOI":"10.1128\/JB.187.9.3122-3132.2005","volume":"187","author":"Burgdorf","year":"2005","journal-title":"J. Bacteriol."},{"key":"10.1074\/jbc.M109.028795_bib62","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1007\/s00239-006-0001-6","volume":"63","author":"Ludwig","year":"2006","journal-title":"J. Mol. Evol."},{"key":"10.1074\/jbc.M109.028795_bib63","doi-asserted-by":"crossref","first-page":"10103","DOI":"10.1021\/ja982053a","volume":"120","author":"Lai","year":"1998","journal-title":"J. Am. Chem. Soc."},{"key":"10.1074\/jbc.M109.028795_bib64","doi-asserted-by":"crossref","first-page":"6457","DOI":"10.1002\/anie.200902695","volume":"48","author":"Hiromoto","year":"2009","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1074\/jbc.M109.028795_bib65","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1007\/s00775-006-0185-7","volume":"12","author":"Schr\u00f6der","year":"2007","journal-title":"J. Biol. Inorg. Chem."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820374573?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820374573?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M109.028795","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T08:46:46Z","timestamp":1641286006000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820374573"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12]]},"references-count":65,"journal-issue":{"issue":"52","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["S0021925820374573"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m109.028795","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2009,12]]}}}