{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T22:15:46Z","timestamp":1775513746229,"version":"3.50.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"19","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"vor","delay-in-days":3157,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2012,5]]},"DOI":"10.1074\/jbc.m112.351213","type":"journal-article","created":{"date-parts":[[2012,3,22]],"date-time":"2012-03-22T15:57:26Z","timestamp":1332431846000},"page":"15371-15379","source":"Crossref","is-referenced-by-count":32,"title":["Inhibitory Mechanism of Caspase-6 Phosphorylation Revealed by Crystal Structures, Molecular Dynamics Simulations, and Biochemical Assays"],"prefix":"10.1016","volume":"287","author":[{"given":"Qin","family":"Cao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Jun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng-Wen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dai-Fei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan-Fen","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Qin","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Dong","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M112.351213_bib1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1146\/annurev.cellbio.21.012704.131040","article-title":"Mechanisms of apoptosis through structural biology","volume":"21","author":"Yan","year":"2005","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1074\/jbc.M112.351213_bib2","doi-asserted-by":"crossref","first-page":"21777","DOI":"10.1074\/jbc.R800084200","article-title":"Human caspases: activation, specificity, and regulation","volume":"284","author":"Pop","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.351213_bib3","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1083\/jcb.144.2.281","article-title":"Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner","volume":"144","author":"Slee","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1074\/jbc.M112.351213_bib4","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.bbamcr.2008.12.004","article-title":"Self-activation of caspase-6 in vitro and in vivo: caspase-6 activation does not induce cell death in HEK293T cells","volume":"1793","author":"Klaiman","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M112.351213_bib5","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/embor.2010.141","article-title":"Crystal structures of human caspase 6 reveal a new mechanism for intramolecular cleavage self-activation","volume":"11","author":"Wang","year":"2010","journal-title":"EMBO Rep."},{"key":"10.1074\/jbc.M112.351213_bib6","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.tins.2011.09.001","article-title":"Caspase-6 and neurodegeneration","volume":"34","author":"Graham","year":"2011","journal-title":"Trends Neurosci."},{"key":"10.1074\/jbc.M112.351213_bib7","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1016\/j.cell.2006.04.026","article-title":"Cleavage at the caspase-6 site is required for neuronal dysfunction and degeneration due to mutant huntingtin","volume":"125","author":"Graham","year":"2006","journal-title":"Cell"},{"key":"10.1074\/jbc.M112.351213_bib8","doi-asserted-by":"crossref","first-page":"15019","DOI":"10.1523\/JNEUROSCI.2071-10.2010","article-title":"Cleavage at the 586-amino acid caspase-6 site in mutant huntingtin influences caspase-6 activation in vivo","volume":"30","author":"Graham","year":"2010","journal-title":"J. Neurosci."},{"key":"10.1074\/jbc.M112.351213_bib9","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.2353\/ajpath.2007.060974","article-title":"Activation of caspase-6 in aging and mild cognitive impairment","volume":"170","author":"Albrecht","year":"2007","journal-title":"Am. J. Pathol."},{"key":"10.1074\/jbc.M112.351213_bib10","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/S0002-9440(10)63317-2","article-title":"Active caspase-6 and caspase-6-cleaved tau in neurophil threads, neuritic plaques, and neurofibrillary tangles of Alzheimer's disease","volume":"165","author":"Guo","year":"2004","journal-title":"Am. J. Pathol."},{"key":"10.1074\/jbc.M112.351213_bib11","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1038\/nature07767","article-title":"APP binds DR6 to trigger axon pruning and neuron death via distinct caspases","volume":"457","author":"Nikolaev","year":"2009","journal-title":"Nature"},{"key":"10.1074\/jbc.M112.351213_bib12","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1074\/mcp.M800007-MCP200","article-title":"Targets of caspase-6 activity in human neurons and Alzheimer disease","volume":"7","author":"Klaiman","year":"2008","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/jbc.M112.351213_bib13","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.cell.2009.08.021","article-title":"Caspases and kinases in a death grip","volume":"138","author":"Kurokawa","year":"2009","journal-title":"Cell"},{"key":"10.1074\/jbc.M112.351213_bib14","doi-asserted-by":"crossref","first-page":"7067","DOI":"10.1038\/sj.onc.1207963","article-title":"Regulation of caspase-6 and FLIP by the AMPK family member ARK5","volume":"23","author":"Suzuki","year":"2004","journal-title":"Oncogene"},{"key":"10.1074\/jbc.M112.351213_bib15","doi-asserted-by":"crossref","first-page":"31974","DOI":"10.1074\/jbc.M110.152744","article-title":"Alternatively spliced caspase-6B isoform inhibits the activation of caspase-6A","volume":"285","author":"Lee","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.351213_bib16","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1042\/BJ20090540","article-title":"The crystal structure of caspase-6, a selective effector of axonal degeneration","volume":"423","author":"Baumgartner","year":"2009","journal-title":"Biochem. J."},{"key":"10.1074\/jbc.M112.351213_bib17","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jmb.2010.11.031","article-title":"Substrate-induced conformational changes occur in all cleaved forms of caspase-6","volume":"406","author":"Vaidya","year":"2011","journal-title":"J. Mol. Biol."},{"key":"10.1074\/jbc.M112.351213_bib18","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.jmb.2011.05.020","article-title":"A new apo-caspase-6 crystal form reveals the active conformation of the apoenzyme","volume":"410","author":"M\u00fcller","year":"2011","journal-title":"J. Mol. Biol."},{"key":"10.1074\/jbc.M112.351213_bib19","doi-asserted-by":"crossref","first-page":"5244","DOI":"10.1016\/j.bmcl.2011.07.041","article-title":"Structure of human caspase-6 in complex with Z-VAD-FMK: new peptide binding mode observed for the non-canonical caspase conformation","volume":"21","author":"M\u00fcller","year":"2011","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"10.1074\/jbc.M112.351213_bib20","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1107\/S0907444909047337","article-title":"XDS","volume":"66","author":"Kabsch","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1074\/jbc.M112.351213_bib21","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of x-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Macromol. Crystallography A"},{"key":"10.1074\/jbc.M112.351213_bib22","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1074\/jbc.M112.351213_bib23","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta Crystallogr. D Biol. Crystallogr."},{"key":"10.1074\/jbc.M112.351213_bib24","series-title":"AMBER 10","author":"Case","year":"2008"},{"key":"10.1074\/jbc.M112.351213_bib25","doi-asserted-by":"crossref","first-page":"13166","DOI":"10.1021\/jp060163v","article-title":"New-generation amber united-atom force field","volume":"110","author":"Yang","year":"2006","journal-title":"J. Phys. Chem. B"},{"key":"10.1074\/jbc.M112.351213_bib26","doi-asserted-by":"crossref","first-page":"6269","DOI":"10.1021\/j100308a038","article-title":"The missing term in effective pair potentials","volume":"91","author":"Berendsen","year":"1987","journal-title":"J. Phys. Chem."},{"key":"10.1074\/jbc.M112.351213_bib27","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/0021-9991(77)90098-5","article-title":"Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes","volume":"23","author":"Ryckaert","year":"1977","journal-title":"J. Comput. Phys."},{"key":"10.1074\/jbc.M112.351213_bib28","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh Ewald: an Nlog(N)method for Ewald sums in large systems","volume":"98","author":"Darden","year":"1993","journal-title":"J. Chem. Phys."},{"key":"10.1074\/jbc.M112.351213_bib29","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/bioinformatics\/bti770","article-title":"The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling","volume":"22","author":"Arnold","year":"2006","journal-title":"Bioinformatics"},{"key":"10.1074\/jbc.M112.351213_bib30","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/sj.cdd.4402260","article-title":"Overlapping cleavage motif selectivity of caspases: implications for analysis of apoptotic pathways","volume":"15","author":"McStay","year":"2008","journal-title":"Cell Death Differ."},{"key":"10.1074\/jbc.M112.351213_bib31","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(00)00129-0","article-title":"Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings","volume":"46","author":"Lipinski","year":"2001","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1074\/jbc.M112.351213_bib32","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1021\/jm900135r","article-title":"Synthesis and in vitro evaluation of sulfonamide isatin Michael acceptors as small molecule inhibitors of caspase-6","volume":"52","author":"Chu","year":"2009","journal-title":"J. Med. Chem."},{"key":"10.1074\/jbc.M112.351213_bib33","doi-asserted-by":"crossref","first-page":"5728","DOI":"10.1021\/jm0601405","article-title":"Design, synthesis, and evaluation of aza-peptide Michael acceptors as selective and potent inhibitors of caspases-2, -3, -6, -7, -8, -9, and -10","volume":"49","author":"Ekici","year":"2006","journal-title":"J. Med. Chem."},{"key":"10.1074\/jbc.M112.351213_bib34","doi-asserted-by":"crossref","first-page":"2933","DOI":"10.1038\/onc.2011.19","article-title":"A new role of NUAK1: directly phosphorylating p53 and regulating cell proliferation","volume":"30","author":"Hou","year":"2011","journal-title":"Oncogene"},{"key":"10.1074\/jbc.M112.351213_bib35","doi-asserted-by":"crossref","first-page":"9492","DOI":"10.1073\/pnas.132241599","article-title":"Apoptotic threshold is lowered by p53 transactivation of caspase-6","volume":"99","author":"MacLachlan","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U.S.A."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820461861?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820461861?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M112.351213","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T15:05:50Z","timestamp":1641308750000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820461861"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5]]},"references-count":35,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["S0021925820461861"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m112.351213","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2012,5]]}}}