{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:25:46Z","timestamp":1791959146861,"version":"4.3.4"},"reference-count":51,"publisher":"Elsevier BV","issue":"43","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"vor","delay-in-days":2906,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2012,10]]},"DOI":"10.1074\/jbc.m112.389676","type":"journal-article","created":{"date-parts":[[2012,8,17]],"date-time":"2012-08-17T04:20:29Z","timestamp":1345177229000},"page":"36147-36157","source":"Crossref","is-referenced-by-count":48,"title":["Cryptococcus neoformans Activates RhoGTPase Proteins Followed by Protein Kinase C, Focal Adhesion Kinase, and Ezrin to Promote Traversal across the Blood-Brain Barrier"],"prefix":"10.1016","volume":"287","author":[{"given":"Jong-Chul","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Crary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun C.","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung J.","family":"Kwon-Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kee J.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M112.389676_bib1","doi-asserted-by":"crossref","first-page":"3206","DOI":"10.1101\/gad.11.23.3206","article-title":"Cryptococcus neoformans mating and virulence are regulated by the G-protein \u03b1 subunit GPA1 and cAMP","volume":"11","author":"Alspaugh","year":"1997","journal-title":"Genes Dev."},{"key":"10.1074\/jbc.M112.389676_bib2","series-title":"Cryptococcus neoformans","author":"Casadevall","year":"1998"},{"key":"10.1074\/jbc.M112.389676_bib3","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.idc.2006.07.001","article-title":"Cryptococcosis","volume":"20","author":"Chayakulkeeree","year":"2006","journal-title":"Infect. Dis. Clin. North Am."},{"key":"10.1074\/jbc.M112.389676_bib4","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1097\/QAD.0b013e328322ffac","article-title":"Estimation of the current global burden of cryptococcal meningitis among persons living with HIV\/AIDS","volume":"23","author":"Park","year":"2009","journal-title":"AIDS"},{"key":"10.1074\/jbc.M112.389676_bib5","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1517\/14656566.9.4.551","article-title":"Epidemiology and management of cryptococcal meningitis. Developments and challenges","volume":"9","author":"Pukkila-Worley","year":"2008","journal-title":"Expert Opin. Pharmacother."},{"key":"10.1074\/jbc.M112.389676_bib6","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1080\/mmy.38.s1.205.213","article-title":"Cryptococcosis. Clinical and biological aspects","volume":"38","author":"Kwon-Chung","year":"2000","journal-title":"Med. Mycol."},{"key":"10.1074\/jbc.M112.389676_bib7","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/CMR.8.4.515","article-title":"Cryptococcosis in the era of AIDS. 100 years after the discovery of Cryptococcus neoformans","volume":"8","author":"Mitchell","year":"1995","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1074\/jbc.M112.389676_bib8","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1146\/annurev.neuro.22.1.11","article-title":"The cell biology of the blood-brain barrier","volume":"22","author":"Rubin","year":"1999","journal-title":"Annu. Rev. Neurosci."},{"key":"10.1074\/jbc.M112.389676_bib9","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nrn1103","article-title":"Neurological diseases. Pathogenesis of bacterial meningitis. From bacteremia to neuronal injury","volume":"4","author":"Kim","year":"2003","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1074\/jbc.M112.389676_bib10","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/nrmicro1952","article-title":"Mechanisms of microbial traversal of the blood-brain barrier","volume":"6","author":"Kim","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1074\/jbc.M112.389676_bib11","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1099\/jmm.0.05230-0","article-title":"Cryptococcus neoformans induces alterations in the cytoskeleton of human brain microvascular endothelial cells","volume":"52","author":"Chen","year":"2003","journal-title":"J. Med. Microbiol."},{"key":"10.1074\/jbc.M112.389676_bib12","doi-asserted-by":"crossref","first-page":"4985","DOI":"10.1128\/IAI.72.9.4985-4995.2004","article-title":"Cryptococcal yeast cells invade the central nervous system via transcellular penetration of the blood-brain barrier","volume":"72","author":"Chang","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1074\/jbc.M112.389676_bib13","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1111\/j.1462-5822.2008.01128.x","article-title":"Involvement of human CD44 during Cryptococcus neoformans infection of brain microvascular endothelial cells","volume":"10","author":"Jong","year":"2008","journal-title":"Cell. Microbiol."},{"key":"10.1074\/jbc.M112.389676_bib14","doi-asserted-by":"crossref","first-page":"1854","DOI":"10.1111\/j.1462-5822.2008.01172.x","article-title":"Invasion of C. neoformans into human brain microvascular endothelial cells requires protein kinase C-\u03b1 activation","volume":"10","author":"Jong","year":"2008","journal-title":"Cell. Microbiol."},{"key":"10.1074\/jbc.M112.389676_bib15","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1042\/bj3480241","article-title":"Rho GTPases and their effector proteins","volume":"348","author":"Bishop","year":"2000","journal-title":"Biochem. J."},{"key":"10.1074\/jbc.M112.389676_bib16","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1074\/jbc.M410176200","article-title":"The 67-kDa laminin receptor promotes CNF1-expressing Escherichia coli K1 internalization into human brain microvascular endothelial cells","volume":"280","author":"Kim","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.389676_bib17","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.bbrc.2005.03.097","article-title":"Escherichia coli outer membrane protein A adheres to human brain microvascular endothelial cells","volume":"330","author":"Shin","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1074\/jbc.M112.389676_bib18","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1038\/sj.onc.1210770","article-title":"Distinct FAK-Src activation events promote \u03b15\u03b21 and \u03b14\u03b21 integrin-stimulated neuroblastoma cell motility","volume":"27","author":"Wu","year":"2008","journal-title":"Oncogene"},{"key":"10.1074\/jbc.M112.389676_bib19","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1083\/jcb.120.1.129","article-title":"Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker","volume":"120","author":"Algrain","year":"1993","journal-title":"J. Cell Biol."},{"key":"10.1074\/jbc.M112.389676_bib20","doi-asserted-by":"crossref","first-page":"16857","DOI":"10.1074\/jbc.M301028200","article-title":"Identification of the receptor for cytotoxic necrotizing factor-1 of Escherichia coli K1","volume":"278","author":"Chung","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.389676_bib21","doi-asserted-by":"crossref","first-page":"e15968","DOI":"10.1371\/journal.pone.0015968","article-title":"Automated analysis of cryptococcal macrophage parasitism using GFP-tagged cryptococci","volume":"5","author":"Voelz","year":"2010","journal-title":"PLoS One"},{"key":"10.1074\/jbc.M112.389676_bib22","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1101\/gad.11.18.2295","article-title":"Rho GTPases and signaling networks","volume":"11","author":"Van Aelst","year":"1997","journal-title":"Genes Dev."},{"key":"10.1074\/jbc.M112.389676_bib23","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1126\/science.279.5350.509","article-title":"Rho GTPases and the actin cytoskeleton","volume":"279","author":"Hall","year":"1998","journal-title":"Science"},{"key":"10.1074\/jbc.M112.389676_bib24","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/S0962-8924(01)02153-5","article-title":"Rho family proteins. Coordinating cell responses","volume":"11","author":"Ridley","year":"2001","journal-title":"Trends Cell Biol."},{"key":"10.1074\/jbc.M112.389676_bib25","doi-asserted-by":"crossref","first-page":"15607","DOI":"10.1074\/jbc.M112224200","article-title":"Cytotoxic necrotizing factor-1 contributes to Escherichia coli K1 invasion of the central nervous system","volume":"277","author":"Khan","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.389676_bib26","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1093\/emboj\/19.4.521","article-title":"Activation of rho through a cross-link with polyamines catalyzed by Bordetella dermonecrotizing toxin","volume":"19","author":"Masuda","year":"2000","journal-title":"EMBO J."},{"key":"10.1074\/jbc.M112.389676_bib27","first-page":"1","article-title":"Bacterial virulence strategies that utilize RhoGTPases","volume":"291","author":"Finlay","year":"2005","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1074\/jbc.M112.389676_bib28","doi-asserted-by":"crossref","first-page":"199","DOI":"10.4161\/cam.5.2.15081","article-title":"Emerging role for ERM proteins in cell adhesion and migration","volume":"5","author":"Arpin","year":"2011","journal-title":"Cell Adh. Migr."},{"key":"10.1074\/jbc.M112.389676_bib29","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1023\/A:1006328303451","article-title":"Protein kinase C targeting in antineoplastic treatment strategies","volume":"17","author":"Jarvis","year":"1999","journal-title":"Invest. New Drugs"},{"key":"10.1074\/jbc.M112.389676_bib30","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.femsim.2004.09.001","article-title":"Current concepts on Escherichia coli K1 translocation of the blood-brain barrier","volume":"42","author":"Xie","year":"2004","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1074\/jbc.M112.389676_bib31","doi-asserted-by":"crossref","first-page":"5074","DOI":"10.1128\/iai.65.12.5074-5081.1997","article-title":"Invasion of brain microvascular endothelial cells by group B streptococci","volume":"65","author":"Nizet","year":"1997","journal-title":"Infect. Immun."},{"key":"10.1074\/jbc.M112.389676_bib32","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1086\/314490","article-title":"Listeria monocytogenes infection and activation of human brain microvascular endothelial cells","volume":"178","author":"Wilson","year":"1998","journal-title":"J. infect. Dis."},{"key":"10.1074\/jbc.M112.389676_bib33","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1086\/502631","article-title":"Mycobacterium tuberculosis invasion and traversal across an in vitro human blood-brain barrier as a pathogenic mechanism for central nervous system tuberculosis","volume":"193","author":"Jain","year":"2006","journal-title":"J. Infect. Dis."},{"key":"10.1074\/jbc.M112.389676_bib34","doi-asserted-by":"crossref","first-page":"4208","DOI":"10.1128\/IAI.67.8.4208-4215.1999","article-title":"Citrobacter freundii invades and replicates in human brain microvascular endothelial cells","volume":"67","author":"Badger","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1074\/jbc.M112.389676_bib35","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1172\/JCI36759","article-title":"Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models","volume":"119","author":"Orihuela","year":"2009","journal-title":"J. Clin. Invest."},{"key":"10.1074\/jbc.M112.389676_bib36","doi-asserted-by":"crossref","first-page":"4536","DOI":"10.1128\/IAI.69.7.4536-4544.2001","article-title":"Traversal of Candida albicans across human blood-brain barrier in vitro","volume":"69","author":"Jong","year":"2001","journal-title":"Infect. Immun."},{"key":"10.1074\/jbc.M112.389676_bib37","doi-asserted-by":"crossref","first-page":"5775","DOI":"10.1128\/IAI.67.11.5775-5783.1999","article-title":"Outer membrane protein A-promoted actin condensation of brain microvascular endothelial cells is required for Escherichia coli invasion","volume":"67","author":"Prasadarao","year":"1999","journal-title":"Infect Immun."},{"key":"10.1074\/jbc.M112.389676_bib38","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s00109-010-0630-5","article-title":"Penetration of the blood-brain barrier by Staphylococcus aureus. Contribution of membrane-anchored lipoteichoic acid","volume":"88","author":"Sheen","year":"2010","journal-title":"J. Mol. Med."},{"key":"10.1074\/jbc.M112.389676_bib40","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1038\/sj.cdd.4401151","article-title":"Hijacking RhoGTPases by protein toxins and apoptosis. Molecular strategies of pathogenic bacteria","volume":"10","author":"Fiorentini","year":"2003","journal-title":"Cell Death Differ."},{"key":"10.1074\/jbc.M112.389676_bib41","doi-asserted-by":"crossref","DOI":"10.1111\/j.1462-5822.2012.01819.x","article-title":"Cryptococcus neoformans phospholipase B1 activates host cell Rac1 for traversal across the blood-brain barrier","author":"Maruvada","year":"2012","journal-title":"Cell. Microbiol."},{"key":"10.1074\/jbc.M112.389676_bib42","doi-asserted-by":"crossref","first-page":"34761","DOI":"10.1074\/jbc.M111.219378","article-title":"Invasion of Cryptococcus neoformans into human brain microvascular endothelial cells is mediated through the lipid rafts-endocytic pathway via the dual specificity tyrosine phosphorylation-regulated kinase 3 (DYRK3)","volume":"286","author":"Huang","year":"2011","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.389676_bib43","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1074\/jbc.M604672200","article-title":"Hyaluronan-CD44 interaction with neural Wiskott-Aldrich syndrome protein (N-WASP) promotes actin polymerization and ErB2 activation leading to \u03b2-catenin nuclear translocation, transcriptional up-regulation, and cell migration in ovarian tumor cells","volume":"282","author":"Bourguignon","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.389676_bib44","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1242\/jcs.115.6.1231","article-title":"Microvilli-like structure are associated with the internalization of virulent capsulated Neisseria meningitides into vascular endothelial cells","volume":"115","author":"Eug\u00e8ne","year":"2002","journal-title":"J. Cell Sci."},{"key":"10.1074\/jbc.M112.389676_bib45","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0898-6568(97)00121-6","article-title":"Protein kinase C and the cytoskeleton","volume":"10","author":"Keenan","year":"1998","journal-title":"Cell. Signal."},{"key":"10.1074\/jbc.M112.389676_bib46","doi-asserted-by":"crossref","first-page":"12253","DOI":"10.1074\/jbc.M110740200","article-title":"Regulation of protein kinase C in Escherichia coli K1 invasion of human brain microvascular endothelial cells","volume":"277","author":"Sukumaran","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M112.389676_bib47","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1128\/IAI.01065-08","article-title":"Evidence of a role for monocytes in dissemination and brain invasion by Cryptococcus neoformans","volume":"77","author":"Charlier","year":"2009","journal-title":"Infect. Immun."},{"key":"10.1074\/jbc.M112.389676_bib48","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1172\/JCI42949","article-title":"Cryptococci at the brain gate. Break and enter or use a Trojan horse?","volume":"120","author":"Casadevall","year":"2010","journal-title":"J. Clin. Invest."},{"key":"10.1074\/jbc.M112.389676_bib49","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1172\/JCI41963","article-title":"Real-time imaging of trapping and urease-dependent transmigration of Cryptococcus neoformans in mouse brain","volume":"120","author":"Shi","year":"2010","journal-title":"J. Clin. Invest."},{"key":"10.1074\/jbc.M112.389676_bib50","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.nbd.2008.08.005","article-title":"Blood-brain barrier tight junction permeability and ischemic stroke","volume":"32","author":"Sandoval","year":"2008","journal-title":"Neurobiol. Dis."},{"key":"10.1074\/jbc.M112.389676_bib51","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1196\/annals.1420.016","article-title":"Regulation of endothelial junctional permeability","volume":"1123","author":"Vandenbroucke","year":"2008","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"10.1074\/jbc.M112.389676_bib39","unstructured":"Deleted in proof"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002192582062590X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002192582062590X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M112.389676","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T06:44:47Z","timestamp":1641365087000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002192582062590X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10]]},"references-count":51,"journal-issue":{"issue":"43","published-print":{"date-parts":[[2012,10]]}},"alternative-id":["S002192582062590X"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m112.389676","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2012,10]]}}}