{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:22:58Z","timestamp":1770481378687,"version":"3.49.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"20","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T00:00:00Z","timestamp":1601596800000},"content-version":"vor","delay-in-days":2711,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2013,5]]},"DOI":"10.1074\/jbc.m112.447441","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T03:16:41Z","timestamp":1364959001000},"page":"14256-14263","source":"Crossref","is-referenced-by-count":21,"title":["Inhibition of Isoleucyl-tRNA Synthetase as a Potential Treatment for Human African Trypanosomiasis"],"prefix":"10.1016","volume":"288","author":[{"given":"Igor","family":"Cestari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Stuart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M112.447441_bib1","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/S0140-6736(09)60829-1","article-title":"Human African trypanosomiasis","volume":"375","author":"Brun","year":"2010","journal-title":"Lancet"},{"key":"10.1074\/jbc.M112.447441_bib2","doi-asserted-by":"crossref","first-page":"e333","DOI":"10.1371\/journal.pntd.0000333","article-title":"The burden of human African trypanosomiasis","volume":"2","author":"F\u00e8vre","year":"2008","journal-title":"PLoS Negl. Trop. Dis"},{"key":"10.1074\/jbc.M112.447441_bib3","doi-asserted-by":"crossref","first-page":"e43913","DOI":"10.1371\/journal.pone.0043913","article-title":"Early invasion of brain parenchyma by African trypanosomes","volume":"7","author":"Frevert","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1074\/jbc.M112.447441_bib4","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1097\/QCO.0b013e32833f9fd0","article-title":"Potential new drugs for human African trypanosomiasis: some progress at last","volume":"23","author":"Barrett","year":"2010","journal-title":"Curr. Opin. Infect. Dis"},{"key":"10.1074\/jbc.M112.447441_bib5","doi-asserted-by":"crossref","first-page":"e55","DOI":"10.1371\/journal.pmed.0050055","article-title":"Eliminating human African trypanosomiasis: where do we stand and what comes next?","volume":"5","author":"Simarro","year":"2008","journal-title":"PLoS Med"},{"key":"10.1074\/jbc.M112.447441_bib6","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.2217\/fmb.11.88","article-title":"Drug resistance in human African trypanosomiasis","volume":"6","author":"Barrett","year":"2011","journal-title":"Future Microbiol"},{"key":"10.1074\/jbc.M112.447441_bib7","doi-asserted-by":"crossref","first-page":"e1454","DOI":"10.1371\/journal.pntd.0001454","article-title":"High prevalence of drug resistance in animal trypanosomes without a history of drug exposure","volume":"5","author":"Chitanga","year":"2011","journal-title":"PLoS Negl. Trop. Dis"},{"key":"10.1074\/jbc.M112.447441_bib8","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1038\/nature10771","article-title":"High-throughput decoding of antitrypanosomal drug efficacy and resistance","volume":"482","author":"Alsford","year":"2012","journal-title":"Nature"},{"key":"10.1074\/jbc.M112.447441_bib9","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1038\/nature08893","article-title":"N-Myristoyltransferase inhibitors as new leads to treat sleeping sickness","volume":"464","author":"Frearson","year":"2010","journal-title":"Nature"},{"key":"10.1074\/jbc.M112.447441_bib10","doi-asserted-by":"crossref","first-page":"e1151","DOI":"10.1371\/journal.pntd.0001151","article-title":"SCYX-7158, an orally-active benzoxaborole for the treatment of stage 2 human African trypanosomiasis","volume":"5","author":"Jacobs","year":"2011","journal-title":"PLoS Negl. Trop. Dis"},{"key":"10.1074\/jbc.M112.447441_bib11","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1038\/nrd2684","article-title":"Genomic-scale prioritization of drug targets: the TDR Targets database","volume":"7","author":"Ag\u00fcero","year":"2008","journal-title":"Nat. Rev. Drug Discov"},{"key":"10.1074\/jbc.M112.447441_bib12","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1096\/fasebj.12.15.1599","article-title":"Aminoacyl-tRNA synthetases as targets for new anti-infectives","volume":"12","author":"Schimmel","year":"1998","journal-title":"FASEB J"},{"key":"10.1074\/jbc.M112.447441_bib13","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.chom.2012.04.015","article-title":"Selective and specific inhibition of the Plasmodium falciparum lysyl-tRNA synthetase by the fungal secondary metabolite cladosporin","volume":"11","author":"Hoepfner","year":"2012","journal-title":"Cell Host Microbe"},{"key":"10.1074\/jbc.M112.447441_bib14","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1016\/j.str.2012.07.011","article-title":"Distinct states of methionyl-tRNA synthetase indicate inhibitor binding by conformational selection","volume":"20","author":"Koh","year":"2012","journal-title":"Structure"},{"key":"10.1074\/jbc.M112.447441_bib15","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1146\/annurev.biochem.69.1.617","article-title":"Aminoacyl-tRNA synthesis","volume":"69","author":"Ibba","year":"2000","journal-title":"Annu. Rev. Biochem"},{"key":"10.1074\/jbc.M112.447441_bib16","doi-asserted-by":"crossref","first-page":"47387","DOI":"10.1074\/jbc.M109089200","article-title":"Structural basis for the recognition of isoleucyl-adenylate and an antibiotic, mupirocin, by isoleucyl-tRNA synthetase","volume":"276","author":"Nakama","year":"2001","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M112.447441_bib17","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1093\/nar\/gkr794","article-title":"Dual targeting of isoleucyl-tRNA synthetase in Trypanosoma brucei is mediated through alternative trans-splicing","volume":"40","author":"Rettig","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/jbc.M112.447441_bib18","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1126\/science.1112181","article-title":"Comparative genomics of trypanosomatid parasitic protozoa","volume":"309","author":"El-Sayed","year":"2005","journal-title":"Science"},{"key":"10.1074\/jbc.M112.447441_bib19","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1172\/JCI33945","article-title":"Kinetoplastids: related protozoan pathogens, different diseases","volume":"118","author":"Stuart","year":"2008","journal-title":"J. Clin. Invest"},{"key":"10.1074\/jbc.M112.447441_bib20","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/nchembio837","article-title":"Drug discovery and development for neglected parasitic diseases","volume":"2","author":"Renslo","year":"2006","journal-title":"Nat. Chem. Biol"},{"key":"10.1074\/jbc.M112.447441_bib21","doi-asserted-by":"crossref","first-page":"14085","DOI":"10.1074\/jbc.M412336200","article-title":"The 14-3-3 proteins of Trypanosoma brucei function in motility, cytokinesis, and cell cycle","volume":"280","author":"Inoue","year":"2005","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M112.447441_bib22","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0166-6851(03)00045-8","article-title":"RNAit: an automated web-based tool for the selection of RNAi targets in Trypanosoma brucei","volume":"128","author":"Redmond","year":"2003","journal-title":"Mol. Biochem. Parasitol"},{"key":"10.1074\/jbc.M112.447441_bib23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0166-6851(99)00002-X","article-title":"A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei","volume":"99","author":"Wirtz","year":"1999","journal-title":"Mol. Biochem. Parasitol"},{"key":"10.1074\/jbc.M112.447441_bib24","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1126\/science.1058955","article-title":"An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei","volume":"291","author":"Schnaufer","year":"2001","journal-title":"Science"},{"key":"10.1074\/jbc.M112.447441_bib25","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1177\/1087057112465980","article-title":"A spectrophotometric assay for quantitative measurement of aminoacyl-tRNA synthetase activity","volume":"18","author":"Cestari","year":"2013","journal-title":"J. Biomol. Screen"},{"key":"10.1074\/jbc.M112.447441_bib26","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ymeth.2007.08.001","article-title":"3\u2032-32P labeling tRNA with nucleotidyltransferase for assaying aminoacylation and peptide bond formation","volume":"44","author":"Ledoux","year":"2008","journal-title":"Methods"},{"key":"10.1074\/jbc.M112.447441_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/nprot.2008.197","article-title":"Protein structure homology modeling using SWISS-MODEL workspace","volume":"4","author":"Bordoli","year":"2009","journal-title":"Nat. Protoc"},{"key":"10.1074\/jbc.M112.447441_bib28","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem"},{"key":"10.1074\/jbc.M112.447441_bib29","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1016\/S0960-894X(01)00127-5","article-title":"Active site of an aminoacyl-tRNA synthetase dissected by energy transfer-dependent fluorescence","volume":"11","author":"Nomanbhoy","year":"2001","journal-title":"Bioorg. Med. Chem. Lett"},{"key":"10.1074\/jbc.M112.447441_bib30","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/nrc1951","article-title":"The NCI60 human tumour cell line anticancer drug screen","volume":"6","author":"Shoemaker","year":"2006","journal-title":"Nat. Rev. Cancer"},{"key":"10.1074\/jbc.M112.447441_bib31","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1001\/jama.1982.03320280052029","article-title":"Duration of vidarabine therapy in biopsy-negative herpes simplex encephalitis","volume":"247","author":"Landry","year":"1982","journal-title":"JAMA"},{"key":"10.1074\/jbc.M112.447441_bib32","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1086\/496896","article-title":"Treatment of African trypanosomiasis with cordycepin and adenosine deaminase inhibitors in a mouse model","volume":"192","author":"Rottenberg","year":"2005","journal-title":"J. Infect. Dis"},{"key":"10.1074\/jbc.M112.447441_bib33","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1073\/pnas.012611299","article-title":"Blocking site-to-site translocation of a misactivated amino acid by mutation of a class I tRNA synthetase","volume":"99","author":"Bishop","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M112.447441_bib34","doi-asserted-by":"crossref","first-page":"10150","DOI":"10.1093\/nar\/gks813","article-title":"Cell cycle-regulated control of VSG expression site silencing by histones and histone chaperones ASF1A and CAF-1b in Trypanosoma brucei","volume":"40","author":"Alsford","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/jbc.M112.447441_bib35","doi-asserted-by":"crossref","DOI":"10.1128\/MCB.00156-13","article-title":"Trypanosome Cdc2-related kinase 9 controls spliced leader RNA cap4 methylation and phosphorylation of the RNA polymerase II subunit RPB1","author":"Badjatia","year":"2013","journal-title":"Mol. Cell. Biol"},{"key":"10.1074\/jbc.M112.447441_bib36","doi-asserted-by":"crossref","first-page":"101","DOI":"10.2165\/00003088-199324020-00002","article-title":"Comparative pharmacokinetics of antiviral nucleoside analogues","volume":"24","author":"Morse","year":"1993","journal-title":"Clin. Pharmacokinet"},{"key":"10.1074\/jbc.M112.447441_bib37","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1128\/AEM.66.3.884-889.2000","article-title":"Overexpression of trigger factor prevents aggregation of recombinant proteins in Escherichia coli","volume":"66","author":"Nishihara","year":"2000","journal-title":"Appl. Environ. Microbiol"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925819545070?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925819545070?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M112.447441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T07:35:59Z","timestamp":1715153759000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925819545070"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5]]},"references-count":37,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["S0021925819545070"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m112.447441","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2013,5]]}}}