{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T23:28:54Z","timestamp":1778628534503,"version":"3.51.4"},"reference-count":52,"publisher":"Elsevier BV","issue":"20","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T00:00:00Z","timestamp":1601596800000},"content-version":"vor","delay-in-days":2711,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2013,5]]},"DOI":"10.1074\/jbc.m113.455402","type":"journal-article","created":{"date-parts":[[2013,4,5]],"date-time":"2013-04-05T00:21:22Z","timestamp":1365121282000},"page":"14276-14286","source":"Crossref","is-referenced-by-count":77,"title":["Alternative Pyrimidine Biosynthesis Protein ApbE Is a Flavin Transferase Catalyzing Covalent Attachment of FMN to a Threonine Residue in Bacterial Flavoproteins"],"prefix":"10.1016","volume":"288","author":[{"given":"Yulia V.","family":"Bertsova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria S.","family":"Fadeeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitaly A.","family":"Kostyrko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina V.","family":"Serebryakova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander A.","family":"Baykov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander V.","family":"Bogachev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M113.455402_bib1","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.bbabio.2009.12.020","article-title":"Sodium-translocating NADH:quinone oxidoreductase as a redox-driven ion pump","volume":"1797","author":"Verkhovsky","year":"2010","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M113.455402_bib2","doi-asserted-by":"crossref","first-page":"16246","DOI":"10.1021\/bi991664s","article-title":"Sequencing and preliminary characterization of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio harveyi","volume":"38","author":"Zhou","year":"1999","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib3","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1128\/MMBR.65.3.353-370.2001","article-title":"Sodium ion cycle in bacterial pathogens. Evidence from cross-genome comparisons","volume":"65","author":"H\u00e4se","year":"2001","journal-title":"Microbiol. Mol. Biol. Rev"},{"key":"10.1074\/jbc.M113.455402_bib4","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/S0014-5793(98)00016-7","article-title":"Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus","volume":"422","author":"Nakayama","year":"1998","journal-title":"FEBS Lett"},{"key":"10.1074\/jbc.M113.455402_bib5","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0014-5793(95)01164-A","article-title":"Predicted structure and possible ion-motive mechanism of the sodium-linked NADH-ubiquinone oxidoreductase of Vibrio alginolyticus","volume":"375","author":"Rich","year":"1995","journal-title":"FEBS Lett"},{"key":"10.1074\/jbc.M113.455402_bib6","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0014-5793(95)00283-F","article-title":"Sequencing and the alignment of structural genes in the nqr operon encoding the Na+-translocating NADH-quinone reductase from Vibrio alginolyticus","volume":"363","author":"Hayashi","year":"1995","journal-title":"FEBS Lett"},{"key":"10.1074\/jbc.M113.455402_bib7","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1016\/j.bbabio.2012.03.017","article-title":"Insights into the mechanism of electron transfer and sodium translocation of the Na+-pumping NADH:quinone oxidoreductase","volume":"1817","author":"Ju\u00e1rez","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M113.455402_bib8","doi-asserted-by":"crossref","first-page":"12322","DOI":"10.1021\/bi048689y","article-title":"Mutagenesis study of the 2Fe-2S center and the FAD binding site of the Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio cholerae","volume":"43","author":"Barquera","year":"2004","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib9","doi-asserted-by":"crossref","first-page":"21349","DOI":"10.1074\/jbc.M311692200","article-title":"NADH oxidation by the Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae. Functional role of the NqrF subunit","volume":"279","author":"T\u00fcrk","year":"2004","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M113.455402_bib10","doi-asserted-by":"crossref","first-page":"27088","DOI":"10.1074\/jbc.M109.071126","article-title":"Localization and function of the membrane-bound riboflavin in the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae","volume":"285","author":"Casutt","year":"2010","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M113.455402_bib11","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0014-5793(00)02404-2","article-title":"FMN is covalently attached to a threonine residue in the NqrB and NqrC subunits of Na+-translocating NADH-quinone reductase from Vibrio alginolyticus","volume":"488","author":"Hayashi","year":"2001","journal-title":"FEBS Lett"},{"key":"10.1074\/jbc.M113.455402_bib12","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1007\/BF00279903","article-title":"Identification of a new class of nitrogen fixation genes in Rhodobacter capsulatus. A putative membrane complex involved in electron transport to nitrogenase","volume":"241","author":"Schmehl","year":"1993","journal-title":"Mol. Gen. Genet"},{"key":"10.1074\/jbc.M113.455402_bib13","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1196\/annals.1419.011","article-title":"Discovery of a ferredoxin:NAD+-oxidoreductase (Rnf) in Acetobacterium woodii. A novel potential coupling site in acetogens","volume":"1125","author":"M\u00fcller","year":"2008","journal-title":"Ann. N.Y. Acad. Sci"},{"key":"10.1074\/jbc.M113.455402_bib14","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1021\/pr049832c","article-title":"Proteome of Methanosarcina acetivorans. Part I. An expanded view of the biology of the cell","volume":"4","author":"Li","year":"2005","journal-title":"J Proteome Res"},{"key":"10.1074\/jbc.M113.455402_bib15","doi-asserted-by":"crossref","first-page":"11273","DOI":"10.1021\/bi800920j","article-title":"Covalent binding of flavins to RnfG and RnfD in the Rnf complex from Vibrio cholerae","volume":"47","author":"Backiel","year":"2008","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib16","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1128\/JB.187.6.1992-2001.2005","article-title":"Functional domains of NosR, a novel transmembrane iron-sulfur flavoprotein necessary for nitrous oxide respiration","volume":"187","author":"Wunsch","year":"2005","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M113.455402_bib17","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1111\/mmi.12067","article-title":"Urocanate reductase. Identification of a novel anaerobic respiratory pathway in Shewanella oneidensis MR-1","volume":"86","author":"Bogachev","year":"2012","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M113.455402_bib18","doi-asserted-by":"crossref","first-page":"3405","DOI":"10.1111\/j.1742-4658.2009.07053.x","article-title":"What's in a covalent bond? On the role and formation of covalently bound flavin cofactors","volume":"276","author":"Heuts","year":"2009","journal-title":"FEBS J"},{"key":"10.1074\/jbc.M113.455402_bib19","doi-asserted-by":"crossref","first-page":"31202","DOI":"10.1074\/jbc.270.52.31202","article-title":"The cytochrome subunit is necessary for covalent FAD attachment to the flavoprotein subunit of p-cresol methylhydroxylase","volume":"270","author":"Kim","year":"1995","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M113.455402_bib20","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1126\/science.1175689","article-title":"SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma","volume":"325","author":"Hao","year":"2009","journal-title":"Science"},{"key":"10.1074\/jbc.M113.455402_bib21","doi-asserted-by":"crossref","first-page":"18418","DOI":"10.1074\/jbc.M111.293803","article-title":"SdhE is a conserved protein required for flavinylation of succinate dehydrogenase in bacteria","volume":"287","author":"McNeil","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M113.455402_bib22","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0014-5793(01)02224-4","article-title":"Expression and mutagenesis of the NqrC subunit of the NQR respiratory Na+ pump from Vibrio cholerae with covalently attached FMN","volume":"492","author":"Barquera","year":"2001","journal-title":"FEBS Lett"},{"key":"10.1074\/jbc.M113.455402_bib23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1471-2180-3-3","article-title":"New knowledge from old. In silico discovery of novel protein domains in Streptomyces coelicolor","volume":"3","author":"Yeats","year":"2003","journal-title":"BMC Microbiol"},{"key":"10.1074\/jbc.M113.455402_bib24","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0014-5793(04)00312-6","article-title":"The origin of the sodium-dependent NADH oxidation by the respiratory chain of Klebsiella pneumoniae","volume":"563","author":"Bertsova","year":"2004","journal-title":"FEBS Lett"},{"key":"10.1074\/jbc.M113.455402_bib25","doi-asserted-by":"crossref","first-page":"7285","DOI":"10.1128\/JB.181.23.7285-7290.1999","article-title":"A periplasmic location is essential for the role of the ApbE lipoprotein in thiamine synthesis in Salmonella typhimurium","volume":"181","author":"Beck","year":"1999","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M113.455402_bib26","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s00203-007-0254-5","article-title":"Regulation of expression of Na+-translocating NADH:quinone oxidoreductase genes in Vibrio harveyiKlebsiella pneumoniae","volume":"188","author":"Fadeeva","year":"2007","journal-title":"Arch. Microbiol"},{"key":"10.1074\/jbc.M113.455402_bib27","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem"},{"key":"10.1074\/jbc.M113.455402_bib28","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1016\/j.bbabio.2012.02.012","article-title":"The single NqrB and NqrC subunits in the Na+-translocating NADH:quinone oxidoreductase (Na+-NQR) from Vibrio cholerae each carry one covalently attached FMN","volume":"1817","author":"Casutt","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M113.455402_bib29","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1074\/jbc.M113.455402_bib30","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/S0959-437X(99)00025-8","article-title":"Selfish operons. The evolutionary impact of gene clustering in prokaryotes and eukaryotes","volume":"9","author":"Lawrence","year":"1999","journal-title":"Curr. Opin. Genet. Dev"},{"key":"10.1074\/jbc.M113.455402_bib31","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.tibtech.2009.01.002","article-title":"Protein function prediction. The power of multiplicity","volume":"27","author":"Rentzsch","year":"2009","journal-title":"Trends Biotechnol"},{"key":"10.1074\/jbc.M113.455402_bib32","first-page":"25","article-title":"Fluorescence analysis of flavoproteins","volume":"131","author":"Munro","year":"1999","journal-title":"Methods Mol. Biol"},{"key":"10.1074\/jbc.M113.455402_bib33","doi-asserted-by":"crossref","first-page":"3181","DOI":"10.1021\/bi00874a016","article-title":"On the existence of spectrally distinct classes of flavoprotein semiquinones. A new method for the quantitative production of flavoprotein semiquinones","volume":"5","author":"Massey","year":"1966","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib34","doi-asserted-by":"crossref","first-page":"36482","DOI":"10.1074\/jbc.M605765200","article-title":"A new flavin radical signal in the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae","volume":"281","author":"Barquera","year":"2006","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M113.455402_bib35","doi-asserted-by":"crossref","first-page":"6291","DOI":"10.1021\/bi900524m","article-title":"Redox properties of the prosthetic groups of Na+-translocating NADH:quinone oxidoreductase. 1. EPR study of the enzyme","volume":"48","author":"Bogachev","year":"2009","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib36","doi-asserted-by":"crossref","first-page":"6299","DOI":"10.1021\/bi900525v","article-title":"Redox properties of the prosthetic groups of Na+-translocating NADH:quinone oxidoreductase. 2. Study of the enzyme by optical spectroscopy","volume":"48","author":"Bogachev","year":"2009","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib37","first-page":"143","article-title":"Na+-Translocating NADH:quinone oxidoreductase. Progress achieved and prospects of investigations","volume":"70","author":"Bogachev","year":"2005","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib38","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1042\/bst0200577","article-title":"Characterization of a sodium-motive NADH:ubiquinone oxidoreductase","volume":"20","author":"Bourne","year":"1992","journal-title":"Biochem. Soc. Trans"},{"key":"10.1074\/jbc.M113.455402_bib39","doi-asserted-by":"crossref","first-page":"7732","DOI":"10.1021\/bi00398a029","article-title":"NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain","volume":"26","author":"Matsushita","year":"1987","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib40","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.1574-6968.2007.01015.x","article-title":"Catalytic properties of Na+-translocating NADH:quinone oxidoreductases from Vibrio harveyiKlebsiella pneumoniaeAzotobacter vinelandii","volume":"279","author":"Fadeeva","year":"2008","journal-title":"FEMS Microbiol. Lett"},{"key":"10.1074\/jbc.M113.455402_bib41","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1128\/JB.180.4.885-891.1998","article-title":"The apbE gene encodes a lipoprotein involved in thiamine synthesis in Salmonella typhimurium","volume":"180","author":"Beck","year":"1998","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M113.455402_bib42","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1002\/prot.20950","article-title":"Crystal structure of the ApbE protein (TM1553) from Thermotoga maritima at 1.58 \u00c5 resolution","volume":"64","author":"Han","year":"2006","journal-title":"Proteins"},{"key":"10.1074\/jbc.M113.455402_bib43","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1128\/JB.00730-10","article-title":"FAD binding by ApbE protein from Salmonella enterica. A new class of FAD-binding proteins","volume":"193","author":"Boyd","year":"2011","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M113.455402_bib44","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1128\/JB.185.1.98-106.2003","article-title":"Lack of the ApbC or ApbE protein results in a defect in Fe-S cluster metabolism in Salmonella enterica serovar Typhimurium","volume":"185","author":"Skovran","year":"2003","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M113.455402_bib45","doi-asserted-by":"crossref","first-page":"9054","DOI":"10.1021\/bi8010253","article-title":"ThiC is an [Fe-S] cluster protein that requires AdoMet to generate the 4-amino-5-hydroxymethyl-2-methylpyrimidine moiety in thiamin synthesis","volume":"47","author":"Martinez-Gomez","year":"2008","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib46","doi-asserted-by":"crossref","first-page":"6291","DOI":"10.1073\/pnas.0501216102","article-title":"Genes required for rapid expression of nitrogenase activity in Azotobacter vinelandii","volume":"102","author":"Curatti","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M113.455402_bib47","doi-asserted-by":"crossref","first-page":"5211","DOI":"10.1128\/JB.182.18.5211-5217.2000","article-title":"The NosX and NirX proteins of Paracoccus denitrificans are functional homologues. Their role in maturation of nitrous oxide reductase","volume":"182","author":"Saunders","year":"2000","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M113.455402_bib48","doi-asserted-by":"crossref","first-page":"38001","DOI":"10.1074\/jbc.M201759200","article-title":"Succinate secreted by Trypanosoma brucei is produced by a novel and unique glycosomal enzyme, NADH-dependent fumarate reductase","volume":"277","author":"Besteiro","year":"2002","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M113.455402_bib52","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.M113.449975","article-title":"The TP0796 lipoprotein of T. pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis","author":"Deka","year":"2013","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M113.455402_bib49","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1128\/jb.170.6.2575-2583.1988","article-title":"A novel suicide vector and its use in construction of insertion mutations. Osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR","volume":"170","author":"Miller","year":"1988","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M113.455402_bib50","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1021\/bi011873o","article-title":"Purification and characterization of the recombinant Na+-translocating NADH:quinone oxidoreductase from Vibrio cholerae","volume":"41","author":"Barquera","year":"2002","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M113.455402_bib51","doi-asserted-by":"crossref","first-page":"824","DOI":"10.2144\/99265bm05","article-title":"The pKNOCK series of broad-host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of gram-negative bacteria","volume":"26","author":"Alexeyev","year":"1999","journal-title":"BioTechniques"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925819545094?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925819545094?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M113.455402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T11:15:18Z","timestamp":1635333318000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925819545094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5]]},"references-count":52,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["S0021925819545094"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m113.455402","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2013,5]]}}}