{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T01:42:03Z","timestamp":1790473323964,"version":"4.1.0"},"reference-count":49,"publisher":"Elsevier BV","issue":"16","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,7]],"date-time":"2020-09-07T00:00:00Z","timestamp":1599436800000},"content-version":"vor","delay-in-days":2351,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1074\/jbc.m114.552281","type":"journal-article","created":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T02:38:11Z","timestamp":1393641491000},"page":"11059-11067","source":"Crossref","is-referenced-by-count":30,"title":["The GTPase-deficient Rab27A(Q78L) Mutant Inhibits Melanosome Transport in Melanocytes through Trapping of Rab27A Effector Protein Slac2-a\/Melanophilin in Their Cytosol"],"prefix":"10.1016","volume":"289","author":[{"given":"Mori\u00e9","family":"Ishida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saki P.","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiko","family":"Ohbayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsunori","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M114.552281_bib1","doi-asserted-by":"crossref","first-page":"2801","DOI":"10.1007\/s00018-008-8351-4","article-title":"Regulation of secretory vesicle traffic by Rab small GTPases","volume":"65","author":"Fukuda","year":"2008","journal-title":"Cell. Mol. Life Sci"},{"key":"10.1074\/jbc.M114.552281_bib2","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1038\/nrm2728","article-title":"Rab GTPases as coordinators of vesicle traffic","volume":"10","author":"Stenmark","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib3","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.ceb.2010.04.007","article-title":"Rab GEFs and GAPs","volume":"22","author":"Barr","year":"2010","journal-title":"Curr. Opin. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib4","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1042\/BSR20100112","article-title":"TBC proteins. GAPs for mammalian small GTPase Rab?","volume":"31","author":"Fukuda","year":"2011","journal-title":"Biosci. Rep"},{"key":"10.1074\/jbc.M114.552281_bib5","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.ceb.2013.04.002","article-title":"Rab GTPase regulation of membrane identity","volume":"25","author":"Pfeffer","year":"2013","journal-title":"Curr. Opin. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib6","first-page":"1473","article-title":"How can mammalian Rab small GTPases be comprehensively analyzed? Development of new tools to comprehensively analyze mammalian Rabs in membrane traffic","volume":"25","author":"Fukuda","year":"2010","journal-title":"Histol. Histopathol"},{"key":"10.1074\/jbc.M114.552281_bib7","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1002\/j.1460-2075.1994.tb06381.x","article-title":"Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis","volume":"13","author":"Stenmark","year":"1994","journal-title":"EMBO J"},{"key":"10.1074\/jbc.M114.552281_bib8","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1111\/tra.12083","article-title":"Rab27 effectors, pleiotropic regulators in secretory pathways","volume":"14","author":"Fukuda","year":"2013","journal-title":"Traffic"},{"key":"10.1074\/jbc.M114.552281_bib9","doi-asserted-by":"crossref","first-page":"11386","DOI":"10.1074\/jbc.M211996200","article-title":"Characterization of the molecular defects in Rab27a, caused by RAB27A missense mutations found in patients with Griscelli syndrome","volume":"278","author":"Bahadoran","year":"2003","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib10","doi-asserted-by":"crossref","first-page":"10238","DOI":"10.1073\/pnas.181336698","article-title":"Mutations in Mlph, encoding a member of the Rab effector family, cause the melanosome transport defects observed in leaden mice","volume":"98","author":"Matesic","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M114.552281_bib11","doi-asserted-by":"crossref","first-page":"9212","DOI":"10.1074\/jbc.M112414200","article-title":"The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain","volume":"277","author":"Kuroda","year":"2002","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib12","doi-asserted-by":"crossref","first-page":"12432","DOI":"10.1074\/jbc.C200005200","article-title":"Slac2-a\/melanophilin, the missing link between Rab27 and myosin Va. Implications of a tripartite protein complex for melanosome transport","volume":"277","author":"Fukuda","year":"2002","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib13","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1038\/ncb760","article-title":"Identification of an organelle receptor for myosin-Va","volume":"4","author":"Wu","year":"2002","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib14","doi-asserted-by":"crossref","first-page":"25423","DOI":"10.1074\/jbc.M202574200","article-title":"A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport","volume":"277","author":"Strom","year":"2002","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib15","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1242\/jcs.01048","article-title":"The small GTPase Rab27B regulates amylase release from rat parotid acinar cells","volume":"117","author":"Imai","year":"2004","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.M114.552281_bib16","doi-asserted-by":"crossref","first-page":"43096","DOI":"10.1074\/jbc.M203862200","article-title":"Slac2-c (synaptotagmin-like protein homologue lacking C2 domains-c), a novel linker protein that interacts with Rab27, myosin Va\/VIIa, and actin","volume":"277","author":"Fukuda","year":"2002","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib17","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1016\/j.str.2008.07.014","article-title":"Structural basis for the exclusive specificity of Slac2-a\/melanophilin for the Rab27 GTPases","volume":"16","author":"Kukimoto-Niino","year":"2008","journal-title":"Structure"},{"key":"10.1074\/jbc.M114.552281_bib18","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/j.1755-148X.2010.00705.x","article-title":"Functional characterization of two RAB27A missense mutations found in Griscelli syndrome type 2","volume":"23","author":"Ohbayashi","year":"2010","journal-title":"Pigment Cell Melanoma Res"},{"key":"10.1074\/jbc.M114.552281_bib19","first-page":"1508","article-title":"Melanoregulin regulates retrograde melanosome transport through interaction with the RILP\u2013p150Glued complex in melanocytes","volume":"125","author":"Ohbayashi","year":"2012","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.M114.552281_bib20","first-page":"2235","article-title":"Rab35 regulates Arf6 activity through centaurin-\u03b22 (ACAP2) during neurite outgrowth","volume":"125","author":"Kobayashi","year":"2012","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.M114.552281_bib21","doi-asserted-by":"crossref","first-page":"22314","DOI":"10.1074\/jbc.M401791200","article-title":"Slac2-a\/melanophilin contains multiple PEST-like sequences that are highly sensitive to proteolysis","volume":"279","author":"Fukuda","year":"2004","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib22","doi-asserted-by":"crossref","first-page":"8963","DOI":"10.1074\/jbc.M111.314385","article-title":"Small GTPase Rab17 regulates dendritic morphogenesis and postsynaptic development of hippocampal neurons","volume":"287","author":"Mori","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib23","doi-asserted-by":"crossref","first-page":"31421","DOI":"10.1074\/jbc.274.44.31421","article-title":"Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X","volume":"274","author":"Fukuda","year":"1999","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib24","first-page":"5177","article-title":"Functional involvement of Rab1A in microtubule-dependent anterograde melanosome transport in melanocytes","volume":"125","author":"Ishida","year":"2012","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.M114.552281_bib25","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1002\/ijc.2910390324","article-title":"A line of non-tumorigenic mouse melanocytes, syngeneic with the B16 melanoma and requiring a tumour promoter for growth","volume":"39","author":"Bennett","year":"1987","journal-title":"Int. J. Cancer"},{"key":"10.1074\/jbc.M114.552281_bib26","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1128\/MCB.23.15.5245-5255.2003","article-title":"The actin-binding domain of Slac2-a\/melanophilin is required for melanosome distribution in melanocytes","volume":"23","author":"Kuroda","year":"2003","journal-title":"Mol. Cell. Biol"},{"key":"10.1074\/jbc.M114.552281_bib27","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/S0076-6879(05)03039-9","article-title":"Analysis of the role of Rab27 effector Slp4-a\/granuphilin-a in dense-core vesicle exocytosis","volume":"403","author":"Fukuda","year":"2005","journal-title":"Methods Enzymol"},{"key":"10.1074\/jbc.M114.552281_bib28","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1006\/jmbi.2001.5072","article-title":"Evolution of the Rab family of small GTP-binding proteins","volume":"313","author":"Pereira-Leal","year":"2001","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M114.552281_bib29","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1111\/j.1600-0854.2011.01216.x","article-title":"Rab27a targeting to melanosomes requires nucleotide exchange but not effector binding","volume":"12","author":"Tarafder","year":"2011","journal-title":"Traffic"},{"key":"10.1074\/jbc.M114.552281_bib30","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1038\/ncb1197","article-title":"Rab27A-binding protein Slp2-a is required for peripheral melanosome distribution and elongated cell shape in melanocytes","volume":"6","author":"Kuroda","year":"2004","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib31","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.1074\/jbc.R600026200","article-title":"Classical nuclear localization signals. Definition, function, and interaction with importin \u03b1","volume":"282","author":"Lange","year":"2007","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib32","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1083\/jcb.102.2.523","article-title":"The amino terminus of the yeast F1-ATPase \u03b2-subunit precursor functions as a mitochondrial import signal","volume":"102","author":"Emr","year":"1986","journal-title":"J. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib33","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1083\/jcb.107.3.897","article-title":"Identification of peroxisomal targeting signals located at the carboxy terminus of four peroxisomal proteins","volume":"107","author":"Gould","year":"1988","journal-title":"J. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib34","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1152\/physiol.00043.2011","article-title":"Mechanisms of protein delivery to melanosomes in pigment cells","volume":"27","author":"Sitaram","year":"2012","journal-title":"Physiology"},{"key":"10.1074\/jbc.M114.552281_bib35","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1083\/jcb.130.4.807","article-title":"Intracellular sorting and targeting of melanosomal membrane proteins. Identification of signals for sorting of the human brown locus protein, gp75","volume":"130","author":"Vijayasaradhi","year":"1995","journal-title":"J. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib36","doi-asserted-by":"crossref","first-page":"12780","DOI":"10.1074\/jbc.274.18.12780","article-title":"A cytoplasmic sequence in human tyrosinase defines a second class of di-leucine-based sorting signals for late endosomal and lysosomal delivery","volume":"274","author":"Calvo","year":"1999","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib37","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1242\/jcs.02930","article-title":"An unconventional dileucine-based motif and a novel cytosolic motif are required for the lysosomal and melanosomal targeting of OA1","volume":"119","author":"Piccirillo","year":"2006","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.M114.552281_bib38","doi-asserted-by":"crossref","first-page":"16831","DOI":"10.1073\/pnas.0407339101","article-title":"dsu functions in a MYO5A-independent pathway to suppress the coat color of dilute mice","volume":"101","author":"O'Sullivan","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M114.552281_bib39","doi-asserted-by":"crossref","first-page":"E2101","DOI":"10.1073\/pnas.1209397109","article-title":"Melanoregulin regulates a shedding mechanism that drives melanosome transfer from melanocytes to keratinocytes","volume":"109","author":"Wu","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M114.552281_bib40","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.bbrc.2012.08.064","article-title":"Melanoregulin is stably targeted to the melanosome membrane by palmitoylation","volume":"426","author":"Wu","year":"2012","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"10.1074\/jbc.M114.552281_bib41","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1242\/jcs.114.6.1091","article-title":"Rab27a enables myosin Va-dependent melanosome capture by recruiting the myosin to the organelle","volume":"114","author":"Wu","year":"2001","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.M114.552281_bib42","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1083\/jcb.152.4.795","article-title":"Rab27a regulates the peripheral distribution of melanosomes in melanocytes","volume":"152","author":"Hume","year":"2001","journal-title":"J. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib43","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1083\/jcb.152.4.843","article-title":"Rab27a. A key to melanosome transport in human melanocytes","volume":"152","author":"Bahadoran","year":"2001","journal-title":"J. Cell Biol"},{"key":"10.1074\/jbc.M114.552281_bib44","doi-asserted-by":"crossref","first-page":"28619","DOI":"10.1074\/jbc.M112.370544","article-title":"The Rab interacting lysosomal protein (RILP) homology domain functions as a novel effector domain for small GTPase Rab36. Rab36 regulates retrograde melanosome transport in melanocytes","volume":"287","author":"Matsui","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib45","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1172\/JCI200318264","article-title":"Griscelli syndrome restricted to hypopigmentation results from a melanophilin defect (GS3) or a MYO5A F-exon deletion (GS1)","volume":"112","author":"M\u00e9nasch\u00e9","year":"2003","journal-title":"J. Clin. Invest"},{"key":"10.1074\/jbc.M114.552281_bib46","doi-asserted-by":"crossref","first-page":"40118","DOI":"10.1074\/jbc.M205765200","article-title":"Synaptotagmin-like protein (Slp) homology domain 1 of Slac2-a\/melanophilin is a critical determinant of GTP-dependent specific binding to Rab27A","volume":"277","author":"Fukuda","year":"2002","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib47","doi-asserted-by":"crossref","first-page":"33854","DOI":"10.1074\/jbc.M111.281394","article-title":"EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells","volume":"286","author":"Imai","year":"2011","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib48","doi-asserted-by":"crossref","first-page":"31823","DOI":"10.1016\/S0021-9258(19)84097-8","article-title":"Identification of EPI64 as a GTPase-activating protein specific for Rab27A","volume":"281","author":"Itoh","year":"2006","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M114.552281_bib49","doi-asserted-by":"crossref","first-page":"28657","DOI":"10.1074\/jbc.M603227200","article-title":"Constitutive GDP\/GTP exchange and secretion-dependent GTP hydrolysis activity for Rab27 in platelets","volume":"281","author":"Kondo","year":"2006","journal-title":"J. Biol. Chem"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820483255?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820483255?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M114.552281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T17:16:40Z","timestamp":1641316600000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820483255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":49,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["S0021925820483255"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m114.552281","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4]]}}}