{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:44:24Z","timestamp":1792640664082,"version":"4.5.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"27","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T00:00:00Z","timestamp":1598832000000},"content-version":"vor","delay-in-days":1888,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["RO1 AR065403"],"award-info":[{"award-number":["RO1 AR065403"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["jbc.org"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2015,7]]},"DOI":"10.1074\/jbc.m115.662635","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T22:41:58Z","timestamp":1432334518000},"page":"16964-16978","update-policy":"https:\/\/doi.org\/10.1074\/jbc.f110.005975","source":"Crossref","is-referenced-by-count":59,"title":["Severe Extracellular Matrix Abnormalities and Chondrodysplasia in Mice Lacking Collagen Prolyl 4-Hydroxylase Isoenzyme II in Combination with a Reduced Amount of Isoenzyme I"],"prefix":"10.1016","volume":"290","author":[{"given":"Ellinoora","family":"Aro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti M.","family":"Salo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richa","family":"Khatri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikko","family":"Finnil\u00e4","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilkka","family":"Miinalainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raija","family":"Sormunen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Outi","family":"Pakkanen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiina","family":"Holster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raija","family":"Soininen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina","family":"Prein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hauke","family":"Clausen-Schaumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Asz\u00f3di","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juha","family":"Tuukkanen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kari I.","family":"Kivirikko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernestina","family":"Schipani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Myllyharju","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M115.662635_bib1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.tig.2003.11.004","article-title":"Collagens, modifying enzymes and their mutations in humans, flies and worms","volume":"20","author":"Myllyharju","year":"2004","journal-title":"Trends Genet"},{"key":"10.1074\/jbc.M115.662635_bib2","doi-asserted-by":"crossref","first-page":"a004978","DOI":"10.1101\/cshperspect.a004978","article-title":"The collagen family","volume":"3","author":"Ricard-Blum","year":"2011","journal-title":"Cold Spring Harb. Perspect. Biol"},{"key":"10.1074\/jbc.M115.662635_bib3","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0945-053X(03)00006-4","article-title":"Prolyl 4-hydroxylases, the key enzymes of collagen biosynthesis","volume":"22","author":"Myllyharju","year":"2003","journal-title":"Matrix Biol"},{"key":"10.1074\/jbc.M115.662635_bib4","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1080\/07853890801986594","article-title":"Prolyl 4-hydroxylases, key enzymes in the synthesis of collagens and regulation of the response to hypoxia, and their roles as treatment targets","volume":"40","author":"Myllyharju","year":"2008","journal-title":"Ann. Med"},{"key":"10.1074\/jbc.M115.662635_bib5","doi-asserted-by":"crossref","first-page":"4392","DOI":"10.1073\/pnas.86.12.4392","article-title":"Molecular cloning of the \u03b1-subunit of human prolyl 4-hydroxylase: the complete cDNA-derived amino acid sequence and evidence for alternative splicing of RNA transcripts","volume":"86","author":"Helaakoski","year":"1989","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.662635_bib6","doi-asserted-by":"crossref","first-page":"4427","DOI":"10.1073\/pnas.92.10.4427","article-title":"Cloning, baculovirus expression, and characterization of a second mouse prolyl 4-hydroxylase \u03b1-subunit isoform: formation of an \u03b12\u03b22 tetramer with the protein disulfide-isomerase\/\u03b2 subunit","volume":"92","author":"Helaakoski","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.662635_bib7","doi-asserted-by":"crossref","first-page":"17342","DOI":"10.1074\/jbc.272.28.17342","article-title":"Cloning of the human prolyl 4-hydroxylase \u03b1 subunit isoform \u03b1(II) and characterization of the type II enzyme tetramer. The \u03b1(I) and \u03b1(II) subunits do not form a mixed \u03b1(I)\u03b1(II)\u03b22 tetramer","volume":"272","author":"Annunen","year":"1997","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.662635_bib8","doi-asserted-by":"crossref","first-page":"47685","DOI":"10.1074\/jbc.M306806200","article-title":"Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme","volume":"278","author":"Kukkola","year":"2003","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.662635_bib9","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1161\/01.CIR.0000080883.53863.5C","article-title":"Cloning of a novel prolyl 4-hydroxylase subunit expressed in the fibrous cap of human atherosclerotic plaque","volume":"108","author":"Van Den Diepstraten","year":"2003","journal-title":"Circulation"},{"key":"10.1074\/jbc.M115.662635_bib10","doi-asserted-by":"crossref","first-page":"5989","DOI":"10.1074\/jbc.273.11.5989","article-title":"The novel type II prolyl 4-hydroxylase is the main enzyme form in chondrocytes and capillary endothelial cells, whereas the type I enzyme predominates in most cells","volume":"273","author":"Annunen","year":"1998","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.662635_bib11","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1177\/002215540104900908","article-title":"Prolyl 4-hydroxylase isoenzymes I and II have different expression patterns in several human tissues","volume":"49","author":"Nissi","year":"2001","journal-title":"J. Histochem. Cytochem"},{"key":"10.1074\/jbc.M115.662635_bib12","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.1074\/jbc.M606608200","article-title":"Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice","volume":"282","author":"Holster","year":"2007","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.662635_bib13","doi-asserted-by":"crossref","first-page":"5300","DOI":"10.1046\/j.0014-2956.2001.02464.x","article-title":"Characterization of the human and mouse genes for the \u03b1 subunit of type II prolyl 4-hydroxylase. Identification of a previously unknown alternatively spliced exon and its expression in various tissues","volume":"268","author":"Nokelainen","year":"2001","journal-title":"Eur. J. Biochem"},{"key":"10.1074\/jbc.M115.662635_bib14","doi-asserted-by":"crossref","first-page":"37134","DOI":"10.1074\/jbc.M112.352872","article-title":"Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes","volume":"287","author":"Aro","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.662635_bib15","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0076-6879(82)82067-3","article-title":"Posttranslational enzymes in the biosynthesis of collagen: intracellular enzymes","volume":"82","author":"Kivirikko","year":"1982","journal-title":"Methods Enzymol"},{"key":"10.1074\/jbc.M115.662635_bib16","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1002\/tera.1420220306","article-title":"Differential staining of cartilage and bone in whole mouse fetuses by alcian blue and alizarin red S","volume":"22","author":"McLeod","year":"1980","journal-title":"Teratology"},{"key":"10.1074\/jbc.M115.662635_bib17","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1002\/jbmr.1487","article-title":"VEGF-independent cell-autonomous functions of HIF-1\u03b1 regulating oxygen consumption in fetal cartilage are critical for chondrocyte survival","volume":"27","author":"Maes","year":"2012","journal-title":"J. Bone Miner. Res"},{"key":"10.1074\/jbc.M115.662635_bib18","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.1152\/japplphysiol.01357.2003","article-title":"Progression of kyphosis in mdx mice","volume":"97","author":"Laws","year":"2004","journal-title":"J. Appl. Physiol"},{"key":"10.1074\/jbc.M115.662635_bib19","doi-asserted-by":"crossref","first-page":"11988","DOI":"10.1074\/jbc.271.20.11988","article-title":"Characterization of human type III collagen expressed in a baculovirus system. Production of a protein with a stable triple helix requires coexpression with the two types of recombinant prolyl 4-hydroxylase subunit","volume":"271","author":"Lamberg","year":"1996","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.662635_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1088\/0957-4484\/6\/1\/001","article-title":"Calculation of thermal noise in atomic force microscopy","volume":"6","author":"Butt","year":"1995","journal-title":"Nanotechnology"},{"key":"10.1074\/jbc.M115.662635_bib21","doi-asserted-by":"crossref","first-page":"3320","DOI":"10.1021\/la9713006","article-title":"Measuring the elastic properties of thin polymer films with the atomic force microscope","volume":"14","author":"Domke","year":"1998","journal-title":"Langmuir"},{"key":"10.1074\/jbc.M115.662635_bib22","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1136\/bmj.300.6719.230","article-title":"Analysis of serial measurements in medical research","volume":"300","author":"Matthews","year":"1990","journal-title":"Brit. Med. J"},{"key":"10.1074\/jbc.M115.662635_bib23","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1101\/gad.277003","article-title":"\u03b21 integrins regulate chondrocyte rotation, G1 progression, and cytokinesis","volume":"17","author":"Aszodi","year":"2003","journal-title":"Genes Dev"},{"key":"10.1074\/jbc.M115.662635_bib24","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1242\/jcs.01678","article-title":"Loss of \u03b110\u03b21 integrin expression leads to moderate dysfunction of growth plate chondrocytes","volume":"118","author":"Bengtsson","year":"2005","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.M115.662635_bib25","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/nrg2520","article-title":"Genetic diseases of connective tissues: cellular and extracellular effects of ECM mutations","volume":"10","author":"Bateman","year":"2009","journal-title":"Nat. Rev. Genet"},{"key":"10.1074\/jbc.M115.662635_bib26","doi-asserted-by":"crossref","first-page":"3731","DOI":"10.1091\/mbc.e12-03-0172","article-title":"ECM stiffness primes the TFG\u03b2 pathway to promote chondrocyte differentiation","volume":"23","author":"Allen","year":"2012","journal-title":"Mol. Biol. Cell"},{"key":"10.1074\/jbc.M115.662635_bib27","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1038\/nrm3896","article-title":"Mechanotransduction and extracellular matrix homeostasis","volume":"15","author":"Humphrey","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol"},{"key":"10.1074\/jbc.M115.662635_bib28","doi-asserted-by":"crossref","first-page":"1728","DOI":"10.1093\/hmg\/ddm121","article-title":"Decreased chondrocyte proliferation and dysregulated apoptosis in the cartilage growth plate are key features of a murine model of epiphyseal dysplasia caused by a matn3 mutation","volume":"16","author":"Leighton","year":"2007","journal-title":"Hum. Mol. Genet"},{"key":"10.1074\/jbc.M115.662635_bib29","doi-asserted-by":"crossref","first-page":"e1000691","DOI":"10.1371\/journal.pgen.1000691","article-title":"Targeted induction of endoplasmic reticulum stress induces cartilage pathology","volume":"5","author":"Rajpar","year":"2009","journal-title":"PLoS Genet"},{"key":"10.1074\/jbc.M115.662635_bib30","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1101\/gad.934301","article-title":"Hypoxia in cartilage: HIF-1\u03b1 is essential for chondrocyte growth arrest and survival","volume":"15","author":"Schipani","year":"2001","journal-title":"Genes Dev"},{"key":"10.1074\/jbc.M115.662635_bib31","doi-asserted-by":"crossref","first-page":"4473","DOI":"10.1242\/dev.083881","article-title":"HIF1\u03b1 is a central regulator of collagen hydroxylation and secretion under hypoxia during bone development","volume":"139","author":"Bentovim","year":"2012","journal-title":"Development"},{"key":"10.1074\/jbc.M115.662635_bib32","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1016\/j.bpj.2010.02.013","article-title":"Micro- and nanomechanical analysis of articular cartilage by indentation-type atomic force microscopy: validation with a gel-microfiber composite","volume":"98","author":"Loparic","year":"2010","journal-title":"Biophys. J"},{"key":"10.1074\/jbc.M115.662635_bib33","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1002\/ajmg.a.33909","article-title":"Nosology and classification of genetic skeletal disorders: 2010 revision","volume":"155A","author":"Warman","year":"2011","journal-title":"Am. J. Med. Genet. A"},{"key":"10.1074\/jbc.M115.662635_bib34","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1101\/gad.9.22.2821","article-title":"Transgenic mice with targeted inactivation of the Col2a1 gene for collagen II develop a skeleton with membranous and periosteal bone but no endochondral bone","volume":"9","author":"Li","year":"1995","journal-title":"Genes Dev"},{"key":"10.1074\/jbc.M115.662635_bib35","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1006\/excr.1997.3692","article-title":"Apoptosis of chondrocytes in transgenic mice lacking collagen II","volume":"235","author":"Yang","year":"1997","journal-title":"Exp. Cell Res"},{"key":"10.1074\/jbc.M115.662635_bib36","doi-asserted-by":"crossref","first-page":"C1504","DOI":"10.1152\/ajpcell.00579.2002","article-title":"Depletion of cartilage collagen fibrils in mice carrying a dominant negative Col2a1 transgene affects chondrocyte differentiation","volume":"285","author":"Barbieri","year":"2003","journal-title":"Am. J. Physiol. Cell. Physiol"},{"key":"10.1074\/jbc.M115.662635_bib37","doi-asserted-by":"crossref","first-page":"5070","DOI":"10.1073\/pnas.91.11.5070","article-title":"9 Mice lacking \u03b11(IX) collagen develop noninflammatory degenerative joint disease","volume":"91","author":"F\u00e4ssler","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.662635_bib38","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.matbio.2008.01.006","article-title":"Collagen IX-deficiency seriously compromises growth cartilage development in mice","volume":"27","author":"Dreier","year":"2008","journal-title":"Matrix Biol"},{"key":"10.1074\/jbc.M115.662635_bib39","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/978-94-017-9153-3_10","article-title":"Cellular signaling by collagen-binding integrins","volume":"819","author":"Heino","year":"2014","journal-title":"Adv. Exp. Med. Biol"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820402509?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820402509?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M115.662635","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T05:27:31Z","timestamp":1641274051000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820402509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7]]},"references-count":39,"journal-issue":{"issue":"27","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["S0021925820402509"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m115.662635","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2015,7]]}}}