{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:11:33Z","timestamp":1791349893185,"version":"4.3.1"},"reference-count":52,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,10]],"date-time":"2020-09-10T00:00:00Z","timestamp":1599696000000},"content-version":"vor","delay-in-days":1654,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["628946"],"award-info":[{"award-number":["628946"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["400202"],"award-info":[{"award-number":["400202"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["FT100100560"],"award-info":[{"award-number":["FT100100560"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2016,3]]},"DOI":"10.1074\/jbc.m115.684258","type":"journal-article","created":{"date-parts":[[2016,1,15]],"date-time":"2016-01-15T12:56:12Z","timestamp":1452862572000},"page":"5128-5137","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":145,"title":["Stimulating the Release of Exosomes Increases the Intercellular Transfer of Prions"],"prefix":"10.1016","volume":"291","author":[{"given":"Belinda B.","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shayne A.","family":"Bellingham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5581-2354","authenticated-orcid":false,"given":"Andrew F.","family":"Hill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M115.684258_bib1","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1038\/nm0598-594","article-title":"Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell-derived exosomes","volume":"4","author":"Zitvogel","year":"1998","journal-title":"Nat. Med"},{"key":"10.1074\/jbc.M115.684258_bib2","doi-asserted-by":"crossref","first-page":"2732","DOI":"10.1182\/blood-2008-05-158642","article-title":"Capture and transfer of HIV-1 particles by mature dendritic cells converges with the exosome-dissemination pathway","volume":"113","author":"Izquierdo-Useros","year":"2009","journal-title":"Blood"},{"key":"10.1074\/jbc.M115.684258_bib3","doi-asserted-by":"crossref","first-page":"124","DOI":"10.3389\/fphys.2012.00124","article-title":"Exosomes: vehicles for the transfer of toxic proteins associated with neurodegenerative diseases?","volume":"3","author":"Bellingham","year":"2012","journal-title":"Front. Physiol"},{"key":"10.1074\/jbc.M115.684258_bib4","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1093\/intimm\/dxh267","article-title":"Exosomal-like vesicles are present in human blood plasma","volume":"17","author":"Caby","year":"2005","journal-title":"Int. Immunol"},{"key":"10.1074\/jbc.M115.684258_bib5","doi-asserted-by":"crossref","first-page":"13368","DOI":"10.1073\/pnas.0403453101","article-title":"Identification and proteomic profiling of exosomes in human urine","volume":"101","author":"Pisitkun","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.684258_bib6","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1084\/jem.183.3.1161","article-title":"B lymphocytes secrete antigen-presenting vesicles","volume":"183","author":"Raposo","year":"1996","journal-title":"J. Exp. Med"},{"key":"10.1074\/jbc.M115.684258_bib7","doi-asserted-by":"crossref","first-page":"e8","DOI":"10.1126\/stke.16pe8","article-title":"Exosomes secreted by bacterially infected macrophages are proinflammatory","volume":"1","author":"O'Neill","year":"2008","journal-title":"Sci. Signal"},{"key":"10.1074\/jbc.M115.684258_bib8","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/ncb1596","article-title":"Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells","volume":"9","author":"Valadi","year":"2007","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/jbc.M115.684258_bib9","doi-asserted-by":"crossref","first-page":"10937","DOI":"10.1093\/nar\/gks832","article-title":"Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells","volume":"40","author":"Bellingham","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/jbc.M115.684258_bib10","doi-asserted-by":"crossref","first-page":"9683","DOI":"10.1073\/pnas.0308413101","article-title":"Cells release prions in association with exosomes","volume":"101","author":"Fevrier","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.684258_bib11","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1002\/path.2145","article-title":"Packaging of prions into exosomes is associated with a novel pathway of PrP processing","volume":"211","author":"Vella","year":"2007","journal-title":"J. Pathol"},{"key":"10.1074\/jbc.M115.684258_bib12","doi-asserted-by":"crossref","first-page":"11172","DOI":"10.1073\/pnas.0603838103","article-title":"Alzheimer's disease \u03b2-amyloid peptides are released in association with exosomes","volume":"103","author":"Rajendran","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.684258_bib13","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1096\/fj.07-9357com","article-title":"Inhibition of \u03b3-secretase causes increased secretion of amyloid precursor protein C-terminal fragments in association with exosomes","volume":"22","author":"Sharples","year":"2008","journal-title":"FASEB J"},{"key":"10.1074\/jbc.M115.684258_bib14","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.nbd.2011.01.029","article-title":"Lysosomal dysfunction increases exosome-mediated \u03b1-synuclein release and transmission","volume":"42","author":"Alvarez-Erviti","year":"2011","journal-title":"Neurobiol. Dis"},{"key":"10.1074\/jbc.M115.684258_bib15","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.neulet.2007.09.024","article-title":"Evidence for secretion of Cu,Zn superoxide dismutase via exosomes from a cell model of amyotrophic lateral sclerosis","volume":"428","author":"Gomes","year":"2007","journal-title":"Neurosci. Lett"},{"key":"10.1074\/jbc.M115.684258_bib16","doi-asserted-by":"crossref","first-page":"4409","DOI":"10.1007\/s00018-015-1945-8","article-title":"Efficient inhibition of infectious prions multiplication and release by targeting the exosomal pathway","volume":"72","author":"Vilette","year":"2015","journal-title":"Cell. Mol. Life Sci"},{"key":"10.1074\/jbc.M115.684258_bib17","doi-asserted-by":"crossref","first-page":"13363","DOI":"10.1073\/pnas.95.23.13363","article-title":"Prions","volume":"95","author":"Prusiner","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.684258_bib18","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/S0960-9822(02)00722-4","article-title":"Transfer of scrapie prion infectivity by cell contact in culture","volume":"12","author":"Kanu","year":"2002","journal-title":"Curr. Biol"},{"key":"10.1074\/jbc.M115.684258_bib19","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1038\/ncb1841","article-title":"Prions hijack tunnelling nanotubes for intercellular spread","volume":"11","author":"Gousset","year":"2009","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/jbc.M115.684258_bib20","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/nature01979","article-title":"RNA molecules stimulate prion protein conversion","volume":"425","author":"Deleault","year":"2003","journal-title":"Nature"},{"key":"10.1074\/jbc.M115.684258_bib21","doi-asserted-by":"crossref","first-page":"8546","DOI":"10.1073\/pnas.1204498109","article-title":"Isolation of phosphatidylethanolamine as a solitary cofactor for prion formation in the absence of nucleic acids","volume":"109","author":"Deleault","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.684258_bib22","doi-asserted-by":"crossref","first-page":"E1938","DOI":"10.1073\/pnas.1206999109","article-title":"Cofactor molecules maintain infectious conformation and restrict strain properties in purified prions","volume":"109","author":"Deleault","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M115.684258_bib23","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1126\/science.1183748","article-title":"Generating a prion with bacterially expressed recombinant prion protein","volume":"327","author":"Wang","year":"2010","journal-title":"Science"},{"key":"10.1074\/jbc.M115.684258_bib24","doi-asserted-by":"crossref","first-page":"2793","DOI":"10.1016\/j.biocel.2008.05.024","article-title":"Mouse-adapted sporadic human Creutzfeldt-Jakob disease prions propagate in cell culture","volume":"40","author":"Lawson","year":"2008","journal-title":"Int. J. Biochem. Cell Biol"},{"key":"10.1074\/jbc.M115.684258_bib25","doi-asserted-by":"crossref","first-page":"3455","DOI":"10.1074\/jbc.M114.605253","article-title":"The neutral sphingomyelinase pathway regulates packaging of the prion protein into exosomes","volume":"290","author":"Guo","year":"2015","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.684258_bib26","doi-asserted-by":"crossref","first-page":"4160","DOI":"10.1096\/fj.11-202077","article-title":"Prion-infected cells regulate the release of exosomes with distinct ultrastructural features","volume":"26","author":"Coleman","year":"2012","journal-title":"FASEB J"},{"key":"10.1074\/jbc.M115.684258_bib27","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol"},{"key":"10.1074\/jbc.M115.684258_bib28","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jim.2008.07.007","article-title":"Discrimination between exosomes and HIV-1: purification of both vesicles from cell-free supernatants","volume":"338","author":"Cantin","year":"2008","journal-title":"J. Immunol. Methods"},{"key":"10.1074\/jbc.M115.684258_bib29","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1182\/blood.V91.7.2573","article-title":"Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during in vitro maturation of reticulocytes","volume":"91","author":"Rabesandratana","year":"1998","journal-title":"Blood"},{"key":"10.1074\/jbc.M115.684258_bib30","doi-asserted-by":"crossref","first-page":"20213","DOI":"10.1074\/jbc.M109.093310","article-title":"Conservation of a glycine-rich region in the prion protein is required for uptake of prion infectivity","volume":"285","author":"Harrison","year":"2010","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.684258_bib31","series-title":"ImageJ","author":"Rasband","year":"1997"},{"key":"10.1074\/jbc.M115.684258_bib32","doi-asserted-by":"crossref","first-page":"8821","DOI":"10.1128\/JVI.71.11.8821-8831.1997","article-title":"A hypothalamic neuronal cell line persistently infected with scrapie prions exhibits apoptosis","volume":"71","author":"Sch\u00e4tzl","year":"1997","journal-title":"J. Virol"},{"key":"10.1074\/jbc.M115.684258_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0896-6273(90)90028-E","article-title":"Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis","volume":"5","author":"Mellon","year":"1990","journal-title":"Neuron"},{"key":"10.1074\/jbc.M115.684258_bib34","doi-asserted-by":"crossref","first-page":"20083","DOI":"10.1074\/jbc.M301642200","article-title":"Exosome release is regulated by a calcium-dependent mechanism in K562 cells","volume":"278","author":"Savina","year":"2003","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.684258_bib35","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1126\/science.1153124","article-title":"Ceramide triggers budding of exosome vesicles into multivesicular endosomes","volume":"319","author":"Trajkovic","year":"2008","journal-title":"Science"},{"key":"10.1074\/jbc.M115.684258_bib36","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1038\/35081095","article-title":"Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding","volume":"411","author":"Saborio","year":"2001","journal-title":"Nature"},{"key":"10.1074\/jbc.M115.684258_bib37","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0304-4157(90)90008-Z","article-title":"Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity","volume":"7","author":"Mollenhauer","year":"1990","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M115.684258_bib38","doi-asserted-by":"crossref","first-page":"1832","DOI":"10.1046\/j.1471-4159.1999.0721832.x","article-title":"Role of endoplasmic reticulum, endosomal-lysosomal compartments, and microtubules in amyloid precursor protein metabolism of human neurons","volume":"72","author":"LeBlanc","year":"1999","journal-title":"J. Neurochem"},{"key":"10.1074\/jbc.M115.684258_bib39","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/BF02331379","article-title":"Effect of monensin on secretory granules and basal \u03b2-endorphin secretion in the melanotroph of the rat pituitary","volume":"28","author":"B\u00e4ck","year":"1996","journal-title":"Histochem. J"},{"key":"10.1074\/jbc.M115.684258_bib40","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1128\/JVI.55.3.813-822.1985","article-title":"Effect of monensin on the assembly of Uukuniemi virus in the Golgi complex","volume":"55","author":"Kuismanen","year":"1985","journal-title":"J. Virol"},{"key":"10.1074\/jbc.M115.684258_bib41","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1038\/emboj.2013.6","article-title":"Post-translational changes to PrP alter transmissible spongiform encephalopathy strain properties","volume":"32","author":"Cancellotti","year":"2013","journal-title":"EMBO J"},{"key":"10.1074\/jbc.M115.684258_bib42","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/383685a0","article-title":"Molecular analysis of prion strain variation and the aetiology of \u201cnew variant\u201d CJD","volume":"383","author":"Collinge","year":"1996","journal-title":"Nature"},{"key":"10.1074\/jbc.M115.684258_bib43","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1016\/S0896-6273(00)80424-9","article-title":"Selective neuronal targeting in prion disease","volume":"19","author":"DeArmond","year":"1997","journal-title":"Neuron"},{"key":"10.1074\/jbc.M115.684258_bib44","doi-asserted-by":"crossref","first-page":"3464","DOI":"10.1128\/JVI.02374-09","article-title":"Glycosylation of PrPC determines timing of neuroinvasion and targeting in the brain following transmissible spongiform encephalopathy infection by a peripheral route","volume":"84","author":"Cancellotti","year":"2010","journal-title":"J. Virol"},{"key":"10.1074\/jbc.M115.684258_bib45","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1091\/mbc.3.8.851","article-title":"Synthesis and trafficking of prion proteins in cultured cells","volume":"3","author":"Taraboulos","year":"1992","journal-title":"Mol. Biol. Cell"},{"key":"10.1074\/jbc.M115.684258_bib46","doi-asserted-by":"crossref","first-page":"10977","DOI":"10.1074\/jbc.M111.324616","article-title":"Sphingolipid-modulated exosome secretion promotes clearance of amyloid-\u03b2 by microglia","volume":"287","author":"Yuyama","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.684258_bib47","doi-asserted-by":"crossref","first-page":"21479","DOI":"10.1074\/jbc.272.34.21479","article-title":"Blockade of glycosylation promotes acquisition of scrapie-like properties by the prion protein in cultured cells","volume":"272","author":"Lehmann","year":"1997","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.684258_bib48","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1002\/ana.410420203","article-title":"Familial spongiform encephalopathy associated with a novel prion protein gene mutation","volume":"42","author":"Nitrini","year":"1997","journal-title":"Ann. Neurol"},{"key":"10.1074\/jbc.M115.684258_bib49","doi-asserted-by":"crossref","first-page":"35878","DOI":"10.1074\/jbc.M704329200","article-title":"High titers of transmissible spongiform encephalopathy infectivity associated with extremely low levels of PrPSc in vivo","volume":"282","author":"Barron","year":"2007","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M115.684258_bib50","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1126\/science.275.5298.402","article-title":"Transmission of the BSE agent to mice in the absence of detectable abnormal prion protein","volume":"275","author":"Lasm\u00e9zas","year":"1997","journal-title":"Science"},{"key":"10.1074\/jbc.M115.684258_bib51","doi-asserted-by":"crossref","first-page":"6855","DOI":"10.1093\/emboj\/18.23.6855","article-title":"A single amino acid alteration (101L) introduced into murine PrP dramatically alters incubation time of transmissible spongiform encephalopathy","volume":"18","author":"Manson","year":"1999","journal-title":"EMBO J"},{"key":"10.1074\/jbc.M115.684258_bib52","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1128\/JVI.66.4.2096-2101.1992","article-title":"Biochemical and physical properties of the prion protein from two strains of the transmissible mink encephalopathy agent","volume":"66","author":"Bessen","year":"1992","journal-title":"J. Virol"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820431515?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820431515?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M115.684258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T06:33:14Z","timestamp":1620541994000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820431515"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":52,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["S0021925820431515"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m115.684258","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Stimulating the Release of Exosomes Increases the Intercellular Transfer of Prions","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.M115.684258","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}