{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:41:02Z","timestamp":1791272462386,"version":"4.1.0"},"reference-count":50,"publisher":"Elsevier BV","issue":"18","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T00:00:00Z","timestamp":1598832000000},"content-version":"vor","delay-in-days":1613,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI034036"],"award-info":[{"award-number":["AI034036"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["15POST22130001"],"award-info":[{"award-number":["15POST22130001"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.1074\/jbc.m115.700518","type":"journal-article","created":{"date-parts":[[2016,3,2]],"date-time":"2016-03-02T03:34:57Z","timestamp":1456889697000},"page":"9554-9565","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":86,"title":["Serum Albumin Stimulates Protein Kinase G-dependent Microneme Secretion in Toxoplasma gondii"],"prefix":"10.1016","volume":"291","author":[{"given":"Kevin M.","family":"Brown","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Lourido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. David","family":"Sibley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M115.700518_bib1","series-title":"Toxoplasmosis of animals and humans","author":"Dubey","year":"2010"},{"key":"10.1074\/jbc.M115.700518_bib2","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/978-0-387-78267-6_2","article-title":"Microneme proteins in apicomplexans","volume":"47","author":"Carruthers","year":"2008","journal-title":"Subcell. Biochem."},{"key":"10.1074\/jbc.M115.700518_bib3","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1016\/j.copbio.2010.05.009","article-title":"How apicomplexan parasites move in and out of cells","volume":"21","author":"Sibley","year":"2010","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1074\/jbc.M115.700518_bib4","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.ceca.2014.12.010","article-title":"The calcium signaling toolkit of the Apicomplexan parasites Toxoplasma gondii and Plasmodium spp","volume":"57","author":"Lourido","year":"2015","journal-title":"Cell Calcium"},{"key":"10.1074\/jbc.M115.700518_bib5","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1046\/j.1365-2958.1999.01174.x","article-title":"Mobilization of intracellular calcium stimulates microneme discharge in Toxoplasma gondii","volume":"31","author":"Carruthers","year":"1999","journal-title":"Mol. Microbiol."},{"key":"10.1074\/jbc.M115.700518_bib6","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1042\/bj3420379","article-title":"Ethanol and acetaldehyde elevate intracellular [Ca2+] calcium and stimulate microneme discharge in Toxoplasma gondii","volume":"342","author":"Carruthers","year":"1999","journal-title":"Biochem. J."},{"key":"10.1074\/jbc.M115.700518_bib7","doi-asserted-by":"crossref","first-page":"25870","DOI":"10.1074\/jbc.M202553200","article-title":"Toxoplasma gondii microneme secretion involves intracellular Ca2+ release from IP3\/ryanodine sensitive stores","volume":"277","author":"Lovett","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M115.700518_bib8","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1038\/nature09022","article-title":"Calcium-dependent protein kinase 1 is an essential regulator of exocytosis in Toxoplasma","volume":"465","author":"Lourido","year":"2010","journal-title":"Nature"},{"key":"10.1074\/jbc.M115.700518_bib9","doi-asserted-by":"crossref","first-page":"4524","DOI":"10.1038\/emboj.2012.299","article-title":"Distinct signalling pathways control Toxoplasma egress and host-cell invasion","volume":"31","author":"Lourido","year":"2012","journal-title":"EMBO J."},{"key":"10.1074\/jbc.M115.700518_bib10","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003049","article-title":"A forward genetic screen reveals that calcium-dependent protein kinase 3 regulates egress in Toxoplasma","volume":"8","author":"Garrison","year":"2012","journal-title":"PLoS Pathog."},{"key":"10.1074\/jbc.M115.700518_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003066","article-title":"TgCDPK3 regulates calcium-dependent egress of Toxoplasma gondii from host cells","volume":"8","author":"McCoy","year":"2012","journal-title":"PLoS Pathog."},{"key":"10.1074\/jbc.M115.700518_bib12","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1046\/j.1462-5822.2003.00305.x","article-title":"Role of the parafusin orthologue, PRP1, in microneme exocytosis and cell invasion in Toxoplasma gondii","volume":"5","author":"Matthiesen","year":"2003","journal-title":"Cell Microbiol."},{"key":"10.1074\/jbc.M115.700518_bib13","doi-asserted-by":"crossref","first-page":"10568","DOI":"10.1073\/pnas.1105622108","article-title":"Chemical genetic screen identifies Toxoplasma DJ-1 as a regulator of parasite secretion, attachment, and invasion","volume":"108","author":"Hall","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1074\/jbc.M115.700518_bib14","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1126\/science.1210829","article-title":"A DOC2 protein identified by mutational profiling is essential for apicomplexan parasite exocytosis","volume":"335","author":"Farrell","year":"2012","journal-title":"Science"},{"key":"10.1074\/jbc.M115.700518_bib15","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.ijpara.2003.11.019","article-title":"A role for coccidian cGMP-dependent protein kinase in motility and invasion","volume":"34","author":"Wiersma","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib16","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1021\/cb501004q","article-title":"Identification of potent phosphodiesterase inhibitors that demonstrate cyclic nucleotide-dependent functions in apicomplexan parasites","volume":"10","author":"Howard","year":"2015","journal-title":"ACS Chem. Biol."},{"key":"10.1074\/jbc.M115.700518_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003344","article-title":"Malaria parasite cGMP-dependent protein kinase regulates blood stage merozoite secretory organelle discharge and egress","volume":"9","author":"Collins","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1074\/jbc.M115.700518_bib18","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1128\/EC.1.3.317-328.2002","article-title":"Toxoplasma gondii cyclic GMP-dependent kinase: chemotherapeutic targeting of an essential parasite protein kinase","volume":"1","author":"Donald","year":"2002","journal-title":"Eukaryot. Cell"},{"key":"10.1074\/jbc.M115.700518_bib19","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001806","article-title":"Phosphoinositide metabolism links cGMP-dependent protein kinase G to essential Ca2+ signals at key decision points in the life cycle of malaria parasites","volume":"12","author":"Brochet","year":"2014","journal-title":"PLos Biol."},{"key":"10.1074\/jbc.M115.700518_bib20","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1046\/j.1462-5822.1999.00023.x","article-title":"Secretion of micronemal proteins is associated with Toxoplasma invasion of host cells","volume":"1","author":"Carruthers","year":"1999","journal-title":"Cell Microbiol."},{"key":"10.1074\/jbc.M115.700518_bib21","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1006\/expr.2000.4585","article-title":"Toxoplasma gondii: molecular cloning and characterization of a novel 18-kDa secretory antigen, TgMIC10","volume":"97","author":"Hoff","year":"2001","journal-title":"Exp. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib22","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1093\/emboj\/cdg217","article-title":"Rapid invasion of host cells by Toxoplasma requires secretion of the MIC2-M2AP adhesive protein complex","volume":"22","author":"Huynh","year":"2003","journal-title":"EMBO J."},{"key":"10.1074\/jbc.M115.700518_bib23","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0166-6851(00)00280-2","article-title":"Induction of secretion and surface capping of microneme proteins in Eimeria tenella","volume":"110","author":"Bumstead","year":"2000","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib24","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1152\/physrev.1999.79.4.1089","article-title":"Measurement of intracellular calcium","volume":"79","author":"Takahashi","year":"1999","journal-title":"Physiol. Rev."},{"key":"10.1074\/jbc.M115.700518_bib25","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0166-6851(96)02796-X","article-title":"Molecular characterisation of an expressed sequence tag locus of Toxoplasma gondii encoding the micronemal protein MIC2","volume":"84","author":"Wan","year":"1997","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib26","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1242\/jcs.115.15.3049","article-title":"Host cells: mobilizable lipid resources for the intracellular parasite Toxoplasma gondii","volume":"115","author":"Charron","year":"2002","journal-title":"J. Cell Sci."},{"key":"10.1074\/jbc.M115.700518_bib27","doi-asserted-by":"crossref","first-page":"9631","DOI":"10.1073\/pnas.1015338108","article-title":"Virulence differences in Toxoplasma mediated by amplification of a family of polymorphic pseuodokinases","volume":"108","author":"Behnke","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1074\/jbc.M115.700518_bib28","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1128\/EC.00358-08","article-title":"Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80","volume":"8","author":"Huynh","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"10.1074\/jbc.M115.700518_bib29","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1126\/science.8469986","article-title":"Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii","volume":"260","author":"Soldati","year":"1993","journal-title":"Science"},{"key":"10.1074\/jbc.M115.700518_bib30","doi-asserted-by":"crossref","first-page":"e01114","DOI":"10.1128\/mBio.01114-14","article-title":"Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/CAS9","volume":"5","author":"Shen","year":"2014","journal-title":"mBio"},{"key":"10.1074\/jbc.M115.700518_bib31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0100450","article-title":"Efficient genome engineering of Toxoplasma gondii using CRISPR\/Cas9","volume":"9","author":"Sidik","year":"2014","journal-title":"PloS One"},{"key":"10.1074\/jbc.M115.700518_bib32","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.chom.2014.04.002","article-title":"ROP18 and ROP17 kinase complexes synergize to control acute virulence of Toxoplasma in the mouse","volume":"15","author":"Etheridge","year":"2014","journal-title":"Cell Host Microbe"},{"key":"10.1074\/jbc.M115.700518_bib33","doi-asserted-by":"crossref","first-page":"4378","DOI":"10.1021\/ac025742k","article-title":"Recombinant Gaussia luciferase: overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization","volume":"74","author":"Verhaegent","year":"2002","journal-title":"Anal. Chem."},{"key":"10.1074\/jbc.M115.700518_bib34","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.ymthe.2004.10.016","article-title":"Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo","volume":"11","author":"Tannous","year":"2005","journal-title":"Mol. Ther."},{"key":"10.1074\/jbc.M115.700518_bib35","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1074\/mcp.R200007-MCP200","article-title":"The human plasma proteome: history, character, and diagnostic prospects","volume":"1","author":"Anderson","year":"2002","journal-title":"Mol. Cell Proteomics"},{"key":"10.1074\/jbc.M115.700518_bib36","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1038\/nature12354","article-title":"Ultrasensitive fluorescent proteins for imaging neuronal activity","volume":"499","author":"Chen","year":"2013","journal-title":"Nature"},{"key":"10.1074\/jbc.M115.700518_bib37","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0166-6851(01)00451-0","article-title":"Molecular characterization of a coccidian parasite cGMP dependent protein kinase","volume":"120","author":"Donald","year":"2002","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib38","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.molbiopara.2006.05.003","article-title":"Anticoccidial kinase inhibitors: identification of protein kinase targets secondary to cGMP-dependent protein kinase","volume":"149","author":"Donald","year":"2006","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib39","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.ijpara.2009.06.011","article-title":"Initiation of Plasmodium sporozoite motility by albumin is associated with induction of intracellular signalling","volume":"40","author":"Kebaier","year":"2010","journal-title":"Int. J. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib40","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1083\/jcb.147.5.937","article-title":"Conservation of a gliding motility and cell invasion machinery in apicomplexan parasites","volume":"147","author":"Kappe","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1074\/jbc.M115.700518_bib41","doi-asserted-by":"crossref","first-page":"3009","DOI":"10.1242\/jcs.00596","article-title":"Intracellular calcium stores in Toxoplasma gondii govern invasion of host cells","volume":"116","author":"Lovett","year":"2003","journal-title":"J. Cell Sci."},{"key":"10.1074\/jbc.M115.700518_bib42","article-title":"Calcium signaling throughout the Toxoplasma gondii lytic cycle: a study using genetically encoded calcium indicators","volume":"291","author":"Borges-Pereira","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1074\/jbc.M115.700518_bib43","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1111\/j.1550-7408.1974.tb03693.x","article-title":"Studies on the motility of Plasmodium sporozoites","volume":"21","author":"Vanderberg","year":"1974","journal-title":"J. Protozool."},{"key":"10.1074\/jbc.M115.700518_bib44","doi-asserted-by":"crossref","first-page":"329","DOI":"10.2307\/3283483","article-title":"Effect of select media supplements on motility and development of Eimeria nieschulzi in vitro","volume":"78","author":"Upton","year":"1992","journal-title":"J. Parasitol."},{"key":"10.1074\/jbc.M115.700518_bib45","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1111\/j.1574-6976.2010.00262.x","article-title":"Cyclic-nucleotide signalling in protozoa","volume":"35","author":"Gould","year":"2011","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1074\/jbc.M115.700518_bib46","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms8285","article-title":"Phosphoproteomics reveals malaria parasite Protein Kinase G as a signalling hub regulating egress and invasion","volume":"6","author":"Alam","year":"2015","journal-title":"Nat. Commun."},{"key":"10.1074\/jbc.M115.700518_bib47","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1128\/EC.00351-09","article-title":"Use of the kinase inhibitor analog 1-NM-PP1 reveals a role for Toxoplasma gondii CDPK1 in the invasion step","volume":"9","author":"Sugi","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"10.1074\/jbc.M115.700518_bib48","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.tips.2015.04.011","article-title":"Designing selective inhibitors for calcium-dependent protein kinases in apicomplexans","volume":"36","author":"Hui","year":"2015","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1074\/jbc.M115.700518_bib49","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.4155\/fmc.12.183","article-title":"Plasmodium kinases as targets for new-generation antimalarials","volume":"4","author":"Lucet","year":"2012","journal-title":"Future Med. Chem."},{"key":"10.1074\/jbc.M115.700518_bib50","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1021\/cb400115y","article-title":"Exploiting the unique ATP-binding pocket of Toxoplasma calcium-dependent protein kinase 1 to identify its substrates","volume":"8","author":"Lourido","year":"2013","journal-title":"ACS Chem. Biol."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820430960?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820430960?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M115.700518","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T12:25:10Z","timestamp":1641299110000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820430960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":50,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["S0021925820430960"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m115.700518","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Serum Albumin Stimulates Protein Kinase G-dependent Microneme Secretion in Toxoplasma gondii","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.M115.700518","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}