{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:40:19Z","timestamp":1773420019060,"version":"3.50.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"42","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T00:00:00Z","timestamp":1599177600000},"content-version":"vor","delay-in-days":1434,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL091799"],"award-info":[{"award-number":["HL091799"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL061690"],"award-info":[{"award-number":["HL061690"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL085503"],"award-info":[{"award-number":["HL085503"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL075443"],"award-info":[{"award-number":["HL075443"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL108806"],"award-info":[{"award-number":["HL108806"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1074\/jbc.m116.721282","type":"journal-article","created":{"date-parts":[[2016,8,27]],"date-time":"2016-08-27T02:20:48Z","timestamp":1472264448000},"page":"21913-21924","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy"],"prefix":"10.1016","volume":"291","author":[{"given":"Benjamin P.","family":"Woodall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meryl C.","family":"Woodall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy S.","family":"Luongo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurel A.","family":"Grisanti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas G.","family":"Tilley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John W.","family":"Elrod","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter J.","family":"Koch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M116.721282_bib1","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1146\/annurev-pharmtox-032112-135923","article-title":"Structure-function of the G protein-coupled receptor superfamily","volume":"53","author":"Katritch","year":"2013","journal-title":"Annu. Rev. Pharmacol. Toxicol"},{"key":"10.1074\/jbc.M116.721282_bib2","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1152\/physrev.00015.2014","article-title":"The evolving impact of G protein-coupled receptor kinases in cardiac health and disease","volume":"95","author":"Sato","year":"2015","journal-title":"Physiol. Rev"},{"key":"10.1074\/jbc.M116.721282_bib3","doi-asserted-by":"crossref","first-page":"1661","DOI":"10.1161\/CIRCRESAHA.114.300513","article-title":"G protein-coupled receptor kinase 2, a link between myocardial contractile function and cardiac metabolism","volume":"114","author":"Woodall","year":"2014","journal-title":"Circ. Res"},{"key":"10.1074\/jbc.M116.721282_bib4","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1126\/science.7761854","article-title":"Cardiac Function in mice overexpressing the \u03b2-adrenergic receptor kinase or a beta ARK inhibitor","volume":"268","author":"Koch","year":"1995","journal-title":"Science"},{"key":"10.1074\/jbc.M116.721282_bib5","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1161\/01.CIR.98.17.1783","article-title":"Reciprocal in vivo regulation of myocardial G protein-coupled receptor kinase expression by \u03b2-adrenergic receptor stimulation and blockade","volume":"98","author":"Iaccarino","year":"1998","journal-title":"Circulation"},{"key":"10.1074\/jbc.M116.721282_bib6","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1016\/S0022-2828(03)00243-8","article-title":"The \u03b2-adrenergic receptor kinase in heart failure","volume":"35","author":"Petrofski","year":"2003","journal-title":"J. Mol. Cell. Cardiol"},{"key":"10.1074\/jbc.M116.721282_bib7","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1161\/01.CIR.87.2.454","article-title":"Altered expression of \u03b2 adrenergic receptor kinase and \u03b2 1-adrenergic receptors in the failing human heart","volume":"87","author":"Ungerer","year":"1993","journal-title":"Circulation"},{"key":"10.1074\/jbc.M116.721282_bib8","doi-asserted-by":"crossref","first-page":"7000","DOI":"10.1073\/pnas.95.12.7000","article-title":"Expression of a \u03b2-adrenergic receptor kinase 1 inhibitor prevents the development of myocardial failure in gene-targeted mice","volume":"95","author":"Rockman","year":"1998","journal-title":"Proc. Natl. Acad. Sci"},{"key":"10.1074\/jbc.M116.721282_bib9","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1161\/01.CIR.100.6.648","article-title":"In vivo inhibition of elevated myocardial \u03b2-adrenergic receptor kinase activity in hybrid transgenic mice restores normal \u03b2-adrenergic signaling and function","volume":"100","author":"Akhter","year":"1999","journal-title":"Circulation"},{"key":"10.1074\/jbc.M116.721282_bib10","doi-asserted-by":"crossref","first-page":"5809","DOI":"10.1073\/pnas.091102398","article-title":"Cardiac \u03b2ARK1 inhibition prolongs survival and augments \u03b2 blocker therapy in a mouse model of severe heart failure","volume":"98","author":"Harding","year":"2001","journal-title":"Proc. Natl. Acad. Sci"},{"key":"10.1074\/jbc.M116.721282_bib11","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1161\/01.CIR.103.9.1311","article-title":"In vivo ventricular gene delivery of a \u03b2-adrenergic receptor kinase inhibitor to the failing heart reverses cardiac dysfunction","volume":"103","author":"Shah","year":"2001","journal-title":"Circulation"},{"key":"10.1074\/jbc.M116.721282_bib12","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1161\/CIRCULATIONAHA.108.803999","article-title":"Myocardial adeno-associated virus serotype 6: \u03b2ARKct gene therapy improves cardiac function and normalizes the neurohormonal axis in chronic heart failure","volume":"119","author":"Rengo","year":"2009","journal-title":"Circulation"},{"key":"10.1074\/jbc.M116.721282_bib13","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1093\/eurheartj\/ehr447","article-title":"AAV6 \u03b2ARKct cardiac gene therapy ameliorates cardiac function and normalizes the catecholaminergic axis in a clinically relevant large animal heart failure model","volume":"34","author":"Raake","year":"2013","journal-title":"Eur. Heart J"},{"key":"10.1074\/jbc.M116.721282_bib14","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1161\/CIRCRESAHA.110.221010","article-title":"Level of G protein-coupled receptor kinase-2 determines myocardial ischemia\/reperfusion injury via pro- and anti-apoptotic mechanisms","volume":"107","author":"Brinks","year":"2010","journal-title":"Circ. Res"},{"key":"10.1074\/jbc.M116.721282_bib15","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1152\/physrev.00028.2007","article-title":"Role of \u03b2-Adrenoceptor signaling in skeletal muscle: implications for muscle wasting and disease","volume":"88","author":"Lynch","year":"2008","journal-title":"Physiol. Rev"},{"key":"10.1074\/jbc.M116.721282_bib16","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1002\/mus.10092","article-title":"Skeletal muscle hypertrophy and anti-atrophy effects of clenbuterol are mediated by the \u03b22-adrenergic receptor","volume":"25","author":"Hinkle","year":"2002","journal-title":"Muscle Nerve"},{"key":"10.1074\/jbc.M116.721282_bib17","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1113\/JP270909","article-title":"\u03b2-Adrenergic modulation of skeletal muscle contraction: key role of excitation-contraction coupling","volume":"593","author":"Cairns","year":"2015","journal-title":"J. Physiol"},{"key":"10.1074\/jbc.M116.721282_bib18","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1161\/01.RES.0000247932.71270.2c","article-title":"Cardiac-specific ablation of G-protein receptor kinase 2 redefines its roles in heart development and \u03b2-adrenergic signaling","volume":"99","author":"Matkovich","year":"2006","journal-title":"Circ. Res"},{"key":"10.1074\/jbc.M116.721282_bib19","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<165::AID-GENE22>3.0.CO;2-F","article-title":"Selective expression of Cre recombinase in skeletal muscle fibers","volume":"26","author":"Bothe","year":"2000","journal-title":"Genesis"},{"key":"10.1074\/jbc.M116.721282_bib20","doi-asserted-by":"crossref","first-page":"26192","DOI":"10.1074\/jbc.M313800200","article-title":"Genetic loss of calcineurin blocks mechanical overload-induced skeletal muscle fiber type switching but not hypertrophy","volume":"279","author":"Parsons","year":"2004","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.721282_bib21","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.cmet.2010.11.008","article-title":"Total skeletal muscle PGC-1 deficiency uncouples mitochondrial derangements from fiber type determination and insulin sensitivity","volume":"12","author":"Zechner","year":"2010","journal-title":"Cell Metab"},{"key":"10.1074\/jbc.M116.721282_bib22","doi-asserted-by":"crossref","first-page":"18180","DOI":"10.1074\/jbc.273.29.18180","article-title":"Control of myocardial contractile function by the level of \u03b2-adrenergic receptor kinase 1 in gene-targeted mice","volume":"273","author":"Rockman","year":"1998","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.721282_bib23","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1161\/CIRCRESAHA.112.300754","article-title":"Prodeath signaling of G protein-coupled receptor kinase 2 in cardiac myocytes after ischemic stress occurs via extracellular signal-regulated kinase-dependent heat shock protein 90-mediated mitochondrial targeting","volume":"112","author":"Chen","year":"2013","journal-title":"Circ. Res"},{"key":"10.1074\/jbc.M116.721282_bib24","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1161\/CIRCULATIONAHA.111.044255","article-title":"Cardiac G-protein-coupled receptor kinase 2 ablation induces a novel Ca2+ handling phenotype resistant to adverse alterations and remodeling after myocardial infarction","volume":"125","author":"Raake","year":"2012","journal-title":"Circulation"},{"key":"10.1074\/jbc.M116.721282_bib25","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/aps.2011.201","article-title":"\u03b2-adrenergic receptor subtype signaling in heart: from bench to bedside","volume":"33","author":"Woo","year":"2012","journal-title":"Acta Pharmacologica Sinica"},{"key":"10.1074\/jbc.M116.721282_bib26","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.yjmcc.2015.10.002","article-title":"GRK2 compromises cardiomyocyte mitochondrial function by diminishing fatty acid-mediated oxygen consumption and increasing superoxide levels","volume":"89","author":"Sato","year":"2015","journal-title":"J. Mol. Cell Cardiol"},{"key":"10.1074\/jbc.M116.721282_bib27","doi-asserted-by":"crossref","first-page":"1953","DOI":"10.1161\/CIRCULATIONAHA.110.988642","article-title":"G protein coupled receptor kinase 2 activity impairs cardiac glucose uptake and promotes insulin resistance after myocardial ischemia","volume":"123","author":"Ciccarelli","year":"2011","journal-title":"Circulation"},{"key":"10.1074\/jbc.M116.721282_bib28","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.2337\/db10-0771","article-title":"G protein-coupled receptor kinase 2 plays a relevant role in insulin resistance and obesity","volume":"59","author":"Garcia-Guerra","year":"2010","journal-title":"Diabetes"},{"key":"10.1074\/jbc.M116.721282_bib29","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/BF01115158","article-title":"The effect of a growth promoting drug, clenbuterol, on fiber frequency and area in hind limb muscles from young male rats","volume":"6","author":"Maltin","year":"1986","journal-title":"Biosci. Rep"},{"key":"10.1074\/jbc.M116.721282_bib30","first-page":"E726","article-title":"Slow to fast alterations in skeletal muscle fibers caused by clenbuterol, a \u03b2-receptor agonist","volume":"254","author":"Zeman","year":"1988","journal-title":"Am. J. Physiol"},{"key":"10.1074\/jbc.M116.721282_bib31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0022-4804(91)90002-4","article-title":"Decreased myofibrillar protein breakdown following treatment with clenbuterol","volume":"50","author":"Benson","year":"1991","journal-title":"J. Surg. Res"},{"key":"10.1074\/jbc.M116.721282_bib32","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1152\/japplphysiol.00873.2006","article-title":"Rapamycin inhibits the growth and muscle-sparing effects of clenbuterol","volume":"102","author":"Kline","year":"2007","journal-title":"J. Appl. Physiol"},{"key":"10.1074\/jbc.M116.721282_bib33","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1002\/mus.20425","article-title":"Systemic administration of l-arginine benefits mdx skeletal muscle function","volume":"32","author":"Barton","year":"2005","journal-title":"Muscle Nerve"},{"key":"10.1074\/jbc.M116.721282_bib34","doi-asserted-by":"crossref","first-page":"e30063","DOI":"10.1371\/journal.pone.0030063","article-title":"Rescue of dystrophic skeletal muscle by PGC-1\u03b1 involves a fast to slow fiber type shift in the mdx mouse","volume":"7","author":"Selsby","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1074\/jbc.M116.721282_bib35","doi-asserted-by":"crossref","first-page":"277ra31","DOI":"10.1126\/scitranslmed.aaa0154","article-title":"Paroxetine-mediated GRK2 inhibition reverses cardiac dysfunction and remodeling after myocardial infarction","volume":"7","author":"Schumacher","year":"2015","journal-title":"Sci. Transl. Med"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820357768?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820357768?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M116.721282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T07:28:04Z","timestamp":1641281284000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820357768"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":35,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["S0021925820357768"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m116.721282","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.M116.721282","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}