{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:52:25Z","timestamp":1790812345401,"version":"4.1.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"23","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,31]],"date-time":"2020-08-31T00:00:00Z","timestamp":1598832000000},"content-version":"vor","delay-in-days":1552,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1074\/jbc.m116.730994","type":"journal-article","created":{"date-parts":[[2016,4,8]],"date-time":"2016-04-08T23:22:06Z","timestamp":1460157726000},"page":"12383-12393","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"title":["VirJ Is a Brucella Virulence Factor Involved in the Secretion of Type IV Secreted Substrates"],"prefix":"10.1016","volume":"291","author":[{"given":"Mariela Giselda","family":"Del Giudice","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Hans","family":"D\u00f6hmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Manuel","family":"Spera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Tom\u00e1s","family":"Laporte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda In\u00e9s","family":"Marchesini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Czibener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Esteban","family":"Ugalde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M116.730994_bib1","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.ceb.2010.05.006","article-title":"Manipulation of host membrane machinery by bacterial pathogens","volume":"22","author":"Cossart","year":"2010","journal-title":"Curr. Opin. Cell Biol"},{"key":"10.1074\/jbc.M116.730994_bib2","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg. Infect. Dis"},{"key":"10.1074\/jbc.M116.730994_bib3","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1046\/j.1462-5822.2001.00102.x","article-title":"Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole","volume":"3","author":"Comerci","year":"2001","journal-title":"Cell Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib4","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1111\/j.1462-5822.2011.01601.x","article-title":"Identification of a Brucella spp. secreted effector specifically interacting with human small GTPase Rab2","volume":"13","author":"de Barsy","year":"2011","journal-title":"Cell Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib5","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1111\/j.1365-2958.2008.06487.x","article-title":"Identification of VceA and VceC, two members of the VjbR regulon that are translocated into macrophages by the Brucella type IV secretion system","volume":"70","author":"de Jong","year":"2008","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib6","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1111\/cmi.12224","article-title":"Identification of a type IV secretion substrate of Brucella abortus that participates in the early stages of intracellular survival","volume":"16","author":"D\u00f6hmer","year":"2014","journal-title":"Cell Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib7","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1111\/j.1462-5822.2011.01618.x","article-title":"In search of Brucella abortus type IV secretion substrates: screening and identification of four proteins translocated into host cells through VirB system","volume":"13","author":"Marchesini","year":"2011","journal-title":"Cell Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib8","doi-asserted-by":"crossref","first-page":"e1003556","DOI":"10.1371\/journal.ppat.1003556","article-title":"Brucella modulates secretory trafficking via multiple type IV secretion effector proteins","volume":"9","author":"Myeni","year":"2013","journal-title":"PLoS Pathog"},{"key":"10.1074\/jbc.M116.730994_bib9","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1046\/j.1365-2958.1999.01569.x","article-title":"A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis","volume":"33","author":"O'Callaghan","year":"1999","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib10","doi-asserted-by":"crossref","first-page":"4849","DOI":"10.1128\/JB.182.17.4849-4855.2000","article-title":"A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication","volume":"182","author":"Sieira","year":"2000","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.730994_bib11","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1128\/MMBR.00023-09","article-title":"Biological diversity of prokaryotic type IV secretion systems","volume":"73","author":"Alvarez-Martinez","year":"2009","journal-title":"Microbiol. Mol. Biol. Rev"},{"key":"10.1074\/jbc.M116.730994_bib12","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1084\/jem.20030088","article-title":"Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum","volume":"198","author":"Celli","year":"2003","journal-title":"J. Exp. Med"},{"key":"10.1074\/jbc.M116.730994_bib13","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1038\/nature13081","article-title":"Structure of a type IV secretion system","volume":"508","author":"Low","year":"2014","journal-title":"Nature"},{"key":"10.1074\/jbc.M116.730994_bib14","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S1369-5274(02)00006-1","article-title":"Type IV transporters of pathogenic bacteria","volume":"6","author":"Burns","year":"2003","journal-title":"Curr. Opin. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib15","doi-asserted-by":"crossref","first-page":"e188","DOI":"10.1371\/journal.ppat.0030188","article-title":"The Legionella pneumophila IcmSW complex interacts with multiple Dot\/Icm effectors to facilitate type IV translocation","volume":"3","author":"Cambronne","year":"2007","journal-title":"PLoS Pathog"},{"key":"10.1074\/jbc.M116.730994_bib16","doi-asserted-by":"crossref","first-page":"5962","DOI":"10.1093\/emboj\/20.21.5962","article-title":"The DotA protein from Legionella pneumophila is secreted by a novel process that requires the Dot\/Icm transporter","volume":"20","author":"Nagai","year":"2001","journal-title":"EMBO J"},{"key":"10.1074\/jbc.M116.730994_bib17","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1046\/j.1365-2958.2002.03098.x","article-title":"Agrobacterium type IV secretion is a two-step process in which export substrates associate with the virulence protein VirJ in the periplasm","volume":"45","author":"Pantoja","year":"2002","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib18","first-page":"730","article-title":"Agrobacterium: nature's genetic engineer","volume":"5","author":"Nester","year":"2014","journal-title":"Front. Plant Sci"},{"key":"10.1074\/jbc.M116.730994_bib19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1365-2958.1995.mmi_17020259.x","article-title":"An Agrobacterium virulence factor encoded by a Ti plasmid gene or a chromosomal gene is required for T-DNA transfer into plants","volume":"17","author":"Pan","year":"1995","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib20","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.micinf.2011.08.012","article-title":"Identification of a unique gene cluster of Brucella spp. that mediates adhesion to host cells","volume":"14","author":"Czibener","year":"2012","journal-title":"Microbes Infect"},{"key":"10.1074\/jbc.M116.730994_bib21","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1128\/IAI.01158-12","article-title":"A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication","volume":"81","author":"Del Giudice","year":"2013","journal-title":"Infect. Immun"},{"key":"10.1074\/jbc.M116.730994_bib22","doi-asserted-by":"crossref","first-page":"6264","DOI":"10.1128\/IAI.71.11.6264-6269.2003","article-title":"Evaluation of Brucella abortus phosphoglucomutase (pgm) mutant as a new live rough-phenotype vaccine","volume":"71","author":"Ugalde","year":"2003","journal-title":"Infect. Immun"},{"key":"10.1074\/jbc.M116.730994_bib23","doi-asserted-by":"crossref","first-page":"4603","DOI":"10.1128\/IAI.67.9.4603-4612.1999","article-title":"Immunization of mice with a TolA-like surface protein of Trypanosoma cruzi generates CD4+ T-cell-dependent parasiticidal activity","volume":"67","author":"Quanquin","year":"1999","journal-title":"Infect. Immun"},{"key":"10.1074\/jbc.M116.730994_bib24","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1128\/JB.00068-15","article-title":"Interaction network and localization of Brucella abortus membrane proteins involved in the synthesis, transport, and succinylation of cyclic \u03b2-1,2-glucans","volume":"197","author":"Guidolin","year":"2015","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.730994_bib25","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/nrmicro3456","article-title":"Secretion systems in Gram-negative bacteria: structural and mechanistic insights","volume":"13","author":"Costa","year":"2015","journal-title":"Nat. Rev. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib26","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1073\/pnas.0406239101","article-title":"A C-terminal translocation signal required for Dot\/Icm-dependent delivery of the Legionella RalF protein to host cells","volume":"102","author":"Nagai","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M116.730994_bib27","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.mib.2012.11.005","article-title":"Brucella T4SS: the VIP pass inside host cells","volume":"16","author":"Lacerda","year":"2013","journal-title":"Curr. Opin. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib28","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3389\/fcimb.2013.00028","article-title":"BtpB, a novel Brucella TIR-containing effector protein with immune modulatory functions","volume":"3","author":"Salcedo","year":"2013","journal-title":"Front. Cell. Infect. Microbiol"},{"key":"10.1074\/jbc.M116.730994_bib29","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1126\/science.1095211","article-title":"Definition of a bacterial type IV secretion pathway for a DNA substrate","volume":"304","author":"Cascales","year":"2004","journal-title":"Science"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820356131?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820356131?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M116.730994","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T02:15:01Z","timestamp":1641262501000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820356131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":29,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["S0021925820356131"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m116.730994","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726275712.793519920","asserted-by":"object"}]},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"VirJ Is a Brucella Virulence Factor Involved in the Secretion of Type IV Secreted Substrates","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.M116.730994","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}