{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:11:34Z","timestamp":1791295894562,"version":"4.1.0"},"reference-count":62,"publisher":"Elsevier BV","issue":"38","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T00:00:00Z","timestamp":1598486400000},"content-version":"vor","delay-in-days":1456,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP 93585"],"award-info":[{"award-number":["MOP 93585"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1074\/jbc.m116.738377","type":"journal-article","created":{"date-parts":[[2016,7,30]],"date-time":"2016-07-30T01:38:38Z","timestamp":1469842718000},"page":"19923-19938","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"title":["Type IV Pilus Alignment Subcomplex Proteins PilN and PilO Form Homo- and Heterodimers in Vivo"],"prefix":"10.1016","volume":"291","author":[{"given":"Tiffany L.","family":"Leighton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel H.","family":"Yong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. Lynne","family":"Howell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0838-5040","authenticated-orcid":false,"given":"Lori L.","family":"Burrows","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M116.738377_bib1","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1139\/m80-022","article-title":"A function of Pseudomonas aeruginosa PAO polar pili: twitching motility","volume":"26","author":"Bradley","year":"1980","journal-title":"Can. J. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib2","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1111\/j.1365-2958.2005.04703.x","article-title":"Weapons of mass retraction","volume":"57","author":"Burrows","year":"2005","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib3","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1146\/annurev-micro-092611-150055","article-title":"Pseudomonas aeruginosa twitching motility: type IV pili in action","volume":"66","author":"Burrows","year":"2012","journal-title":"Annu. Rev. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib4","doi-asserted-by":"crossref","first-page":"4148","DOI":"10.1111\/1462-2920.12849","article-title":"Biogenesis of Pseudomonas aeruginosa type IV pili and regulation of their function","volume":"17","author":"Leighton","year":"2015","journal-title":"Environ. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib5","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1146\/annurev.micro.56.012302.160938","article-title":"Type IV pili and twitching motility","volume":"56","author":"Mattick","year":"2002","journal-title":"Annu. Rev. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib6","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1111\/j.1365-2958.2008.06197.x","article-title":"Type IV pili: e pluribus unum?","volume":"68","author":"Pelicic","year":"2008","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib7","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1146\/annurev.mi.47.100193.003025","article-title":"Structure-function and biogenesis of the type IV pili","volume":"47","author":"Strom","year":"1993","journal-title":"Annu. Rev. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib8","doi-asserted-by":"crossref","first-page":"9977","DOI":"10.1074\/jbc.M110.212688","article-title":"Structure and function of PilQ, a secretin of the DNA transporter from the thermophilic bacterium Thermus thermophilus HB27","volume":"286","author":"Burkhardt","year":"2011","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.738377_bib9","doi-asserted-by":"crossref","first-page":"3825","DOI":"10.1128\/JB.183.13.3825-3832.2001","article-title":"Analysis of the PilQ secretin from Neisseria meningitidis by transmission electron microscopy reveals a dodecameric quaternary structure","volume":"183","author":"Collins","year":"2001","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib10","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1128\/JB.185.8.2611-2617.2003","article-title":"Three-dimensional structure of the Neisseria meningitidis secretin PilQ determined from negative-stain transmission electron microscopy","volume":"185","author":"Collins","year":"2003","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2011.02464.x","article-title":"Decoding the roles of pilotins and accessory proteins in secretin escort services","volume":"328","author":"Koo","year":"2012","journal-title":"FEMS Microbiol. Lett"},{"key":"10.1074\/jbc.M116.738377_bib12","doi-asserted-by":"crossref","first-page":"6961","DOI":"10.1128\/JB.00996-08","article-title":"PilF is an outer membrane lipoprotein required for multimerization and localization of the Pseudomonas aeruginosa type IV pilus secretin","volume":"190","author":"Koo","year":"2008","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib13","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1128\/JB.187.3.829-839.2005","article-title":"Disparate subcellular localization patterns of Pseudomonas aeruginosa type IV pilus ATPases involved in twitching motility","volume":"187","author":"Chiang","year":"2005","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib14","doi-asserted-by":"crossref","first-page":"9721","DOI":"10.1074\/jbc.M113.453506","article-title":"The platform protein is essential for type IV pilus biogenesis","volume":"288","author":"Takhar","year":"2013","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.738377_bib15","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.jmb.2009.10.070","article-title":"Structural characterization of novel Pseudomonas aeruginosa type IV pilins","volume":"395","author":"Nguyen","year":"2010","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib16","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1074\/jbc.M114.616904","article-title":"Pseudomonas aeruginosa minor pilins prime type IVa pilus assembly and promote surface display of the PilY1 adhesin","volume":"290","author":"Nguyen","year":"2015","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.738377_bib17","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jmb.2009.09.034","article-title":"PilM\/N\/O\/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin","volume":"394","author":"Ayers","year":"2009","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib18","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jmb.2009.09.037","article-title":"Periplasmic domains of Pseudomonas aeruginosa PilN and PilO form a stable heterodimeric complex","volume":"394","author":"Sampaleanu","year":"2009","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib19","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1128\/JB.00032-13","article-title":"PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA","volume":"195","author":"Tammam","year":"2013","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib20","doi-asserted-by":"crossref","first-page":"1496","DOI":"10.1111\/j.1365-2958.2011.07903.x","article-title":"Characterization of the PilN, PilO and PilP type IVa pilus subcomplex","volume":"82","author":"Tammam","year":"2011","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib21","doi-asserted-by":"crossref","first-page":"5716","DOI":"10.1128\/JB.00060-07","article-title":"Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis","volume":"189","author":"Balasingham","year":"2007","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib22","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.1128\/JB.184.8.2305-2309.2002","article-title":"Type 4 pilus biogenesis and type II-mediated protein secretion by Vibrio cholerae occur independently of the TonB-facilitated proton motive force","volume":"184","author":"Bose","year":"2002","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib23","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.2217\/fmb.10.76","article-title":"Architecture of the type II secretion and type IV pilus machineries","volume":"5","author":"Ayers","year":"2010","journal-title":"Future Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib24","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1098\/rstb.2011.0204","article-title":"On the path to uncover the bacterial type II secretion system","volume":"367","author":"Douzi","year":"2012","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci"},{"key":"10.1074\/jbc.M116.738377_bib25","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1128\/JB.185.9.2749-2758.2003","article-title":"Type II protein secretion in Pseudomonas aeruginosa: the pseudopilus is a multifibrillar and adhesive structure","volume":"185","author":"Durand","year":"2003","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib26","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.jmb.2004.09.062","article-title":"The structure of the cytoplasmic domain of EpsL, an inner membrane component of the type II secretion system of Vibrio cholerae: an unusual member of the actin-like ATPase superfamily","volume":"344","author":"Abendroth","year":"2004","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib27","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.jsb.2009.07.022","article-title":"The dimer formed by the periplasmic domain of EpsL from the type 2 secretion system of Vibrio parahaemolyticus","volume":"168","author":"Abendroth","year":"2009","journal-title":"J. Struct. Biol"},{"key":"10.1074\/jbc.M116.738377_bib28","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.jmb.2004.01.064","article-title":"The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold","volume":"338","author":"Abendroth","year":"2004","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib29","doi-asserted-by":"crossref","first-page":"E4638","DOI":"10.1073\/pnas.1312313110","article-title":"Structure and assembly of an inner membrane platform for initiation of type IV pilus biogenesis","volume":"110","author":"Karuppiah","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.M116.738377_bib30","doi-asserted-by":"crossref","first-page":"24434","DOI":"10.1074\/jbc.M111.243535","article-title":"Structure of the PilM-PilN inner membrane type IV pilus biogenesis complex from Thermus thermophilus","volume":"286","author":"Karuppiah","year":"2011","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.738377_bib31","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1093\/embo-reports\/kve042","article-title":"An inner membrane platform in the type II secretion machinery of Gram-negative bacteria","volume":"2","author":"Py","year":"2001","journal-title":"EMBO Rep"},{"key":"10.1074\/jbc.M116.738377_bib32","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1128\/JB.181.10.3129-3135.1999","article-title":"Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae","volume":"181","author":"Sandkvist","year":"1999","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib33","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1128\/JB.184.6.1779-1782.2002","article-title":"Identification of XcpZ domains required for assembly of the secreton of Pseudomonas aeruginosa","volume":"184","author":"Robert","year":"2002","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib34","doi-asserted-by":"crossref","first-page":"e79562","DOI":"10.1371\/journal.pone.0079562","article-title":"Dynamic interplay between the periplasmic and transmembrane domains of GspL and GspM in the type II secretion system","volume":"8","author":"Lallemand","year":"2013","journal-title":"PLoS One"},{"key":"10.1074\/jbc.M116.738377_bib35","doi-asserted-by":"crossref","first-page":"12655","DOI":"10.1021\/bi0507884","article-title":"Evidence that the adaptation region of the aspartate receptor is a dynamic four-helix bundle: cysteine and disulfide scanning studies","volume":"44","author":"Winston","year":"2005","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M116.738377_bib36","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/nsmb.1379","article-title":"Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB","volume":"15","author":"Seeger","year":"2008","journal-title":"Nat. Struct. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib37","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0076-6879(07)23002-2","article-title":"Use of site-directed cysteine and disulfide chemistry to probe protein structure and dynamics: applications to soluble and transmembrane receptors of bacterial chemotaxis","volume":"423","author":"Bass","year":"2007","journal-title":"Methods Enzymol"},{"key":"10.1074\/jbc.M116.738377_bib38","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1099\/mic.0.2006\/002840-0","article-title":"Interaction domains in the Pseudomonas aeruginosa type II secretory apparatus component XcpS (GspF)","volume":"153","author":"Arts","year":"2007","journal-title":"Microbiology"},{"key":"10.1074\/jbc.M116.738377_bib39","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.femsle.2005.08.029","article-title":"Subcomplexes from the Xcp secretion system of Pseudomonas aeruginosa","volume":"252","author":"Robert","year":"2005","journal-title":"FEMS Microbiol. Lett"},{"key":"10.1074\/jbc.M116.738377_bib40","doi-asserted-by":"crossref","first-page":"6946","DOI":"10.1074\/jbc.M115.701284","article-title":"The type IV pilus assembly ATPase PilB of Myxococcus xanthus interacts with the inner membrane platform protein PilC and the nucleotide binding protein PilM","volume":"291","author":"Bischof","year":"2016","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.738377_bib41","doi-asserted-by":"crossref","first-page":"11003","DOI":"10.1074\/jbc.M116.718353","article-title":"PilN binding modulates the structure and binding partners of the Pseudomonas aeruginosa type IVa pilus protein PilM","volume":"291","author":"McCallum","year":"2016","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.738377_bib42","doi-asserted-by":"crossref","first-page":"2229","DOI":"10.1128\/JB.00220-15","article-title":"A novel role for PilNO in type IV pilus retraction revealed by alignment subcomplex mutations","volume":"197","author":"Leighton","year":"2015","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib43","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1016\/0022-2836(92)91063-U","article-title":"Thermal motions of surface \u03b1-helices in the d-galactose chemosensory receptor. Detection by disulfide trapping","volume":"226","author":"Careaga","year":"1992","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib44","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0006-3495(92)81806-4","article-title":"Structure and dynamics of Escherichia coli chemosensory receptors. Engineered sulfhydryl studies","volume":"62","author":"Careaga","year":"1992","journal-title":"Biophys. J"},{"key":"10.1074\/jbc.M116.738377_bib45","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/nprot.2009.2","article-title":"Protein structure prediction on the Web: a case study using the Phyre server","volume":"4","author":"Kelley","year":"2009","journal-title":"Nat. Protoc"},{"key":"10.1074\/jbc.M116.738377_bib46","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1046\/j.1365-2958.1998.00935.x","article-title":"PilT mutations lead to simultaneous defects in competence for natural transformation and twitching motility in piliated Neisseria gonorrhoeae","volume":"29","author":"Wolfgang","year":"1998","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib47","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0378-1119(91)90221-V","article-title":"Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria","volume":"101","author":"Whitchurch","year":"1991","journal-title":"Gene"},{"key":"10.1074\/jbc.M116.738377_bib48","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1111\/j.1365-2958.1994.tb00499.x","article-title":"Characterization of a gene, pilU, required for twitching motility but not phage sensitivity in Pseudomonas aeruginosa","volume":"13","author":"Whitchurch","year":"1994","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib49","doi-asserted-by":"crossref","first-page":"aad2001","DOI":"10.1126\/science.aad2001","article-title":"Architecture of the type IVa pilus machine","volume":"351","author":"Chang","year":"2016","journal-title":"Science"},{"key":"10.1074\/jbc.M116.738377_bib50","doi-asserted-by":"crossref","first-page":"19082","DOI":"10.1074\/jbc.M112.346338","article-title":"Cysteine scanning mutagenesis and disulfide mapping analysis of arrangement of GspC and GspD protomers within the type 2 secretion system","volume":"287","author":"Wang","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M116.738377_bib51","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1006\/jmbi.1999.2803","article-title":"Assembly of the type II secretion machinery of Erwinia chrysanthemi: direct interaction and associated conformational change between OutE, the putative ATP-binding component and the membrane protein OutL","volume":"289","author":"Py","year":"1999","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib52","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1002\/j.1460-2075.1995.tb07155.x","article-title":"Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae","volume":"14","author":"Sandkvist","year":"1995","journal-title":"EMBO J"},{"key":"10.1074\/jbc.M116.738377_bib53","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1111\/j.1365-2958.2010.07487.x","article-title":"In vivo cross-linking of EpsG to EpsL suggests a role for EpsL as an ATPase-pseudopilin coupling protein in the type II secretion system of Vibrio cholerae","volume":"79","author":"Gray","year":"2011","journal-title":"Mol. Microbiol"},{"key":"10.1074\/jbc.M116.738377_bib54","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jmb.2012.02.041","article-title":"Structure of an essential type IV pilus biogenesis protein provides insights into pilus and type II secretion systems","volume":"419","author":"Yamagata","year":"2012","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.M116.738377_bib55","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1128\/JB.01094-13","article-title":"Outside-in assembly pathway of the type IV pilus system in Myxococcus xanthus","volume":"196","author":"Friedrich","year":"2014","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib56","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1099\/mic.0.2007\/011320-0","article-title":"Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilU","volume":"154","author":"Chiang","year":"2008","journal-title":"Microbiology"},{"key":"10.1074\/jbc.M116.738377_bib57","doi-asserted-by":"crossref","first-page":"6513","DOI":"10.1128\/JB.00943-09","article-title":"Single-residue changes in the C-terminal disulfide-bonded loop of the Pseudomonas aeruginosa type IV pilin influence pilus assembly and twitching motility","volume":"191","author":"Harvey","year":"2009","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib58","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1099\/mic.0.26822-0","article-title":"Significant differences in type IV pilin allele distribution among Pseudomonas aeruginosa isolates from cystic fibrosis (CF) versus non-CF patients","volume":"150","author":"Kus","year":"2004","journal-title":"Microbiology"},{"key":"10.1074\/jbc.M116.738377_bib59","doi-asserted-by":"crossref","first-page":"7464","DOI":"10.1128\/JB.01075-08","article-title":"Modification of Pseudomonas aeruginosa Pa5196 type IV pilins at multiple sites with d-Araf by a novel GT-C family arabinosyltransferase, TfpW","volume":"190","author":"Kus","year":"2008","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib60","doi-asserted-by":"crossref","first-page":"2914","DOI":"10.1021\/bi3015345","article-title":"Functional mapping of PilF and PilQ in the Pseudomonas aeruginosa type IV pilus system","volume":"52","author":"Koo","year":"2013","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.M116.738377_bib61","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1128\/jb.172.6.2911-2919.1990","article-title":"Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili","volume":"172","author":"Nunn","year":"1990","journal-title":"J. Bacteriol"},{"key":"10.1074\/jbc.M116.738377_bib62","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1128\/aem.53.7.1649-1654.1987","article-title":"In vivo formation of gene fusions in Pseudomonas putida and construction of versatile broad-host-range vectors for direct subcloning of Mu d1 and Mu d2 fusions","volume":"53","author":"Simon","year":"1987","journal-title":"Appl. Environ. Microbiol"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820359718?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820359718?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M116.738377","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,17]],"date-time":"2021-12-17T01:06:18Z","timestamp":1639703178000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820359718"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":62,"journal-issue":{"issue":"38","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["S0021925820359718"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m116.738377","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Type IV Pilus Alignment Subcomplex Proteins PilN and PilO Form Homo- and Heterodimers in Vivo","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.M116.738377","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}