{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:28:24Z","timestamp":1791919704716,"version":"4.3.4"},"reference-count":44,"publisher":"Elsevier BV","issue":"51","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T00:00:00Z","timestamp":1598227200000},"content-version":"vor","delay-in-days":997,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["2IO1BX001355-01A2"],"award-info":[{"award-number":["2IO1BX001355-01A2"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21 AG054975"],"award-info":[{"award-number":["R21 AG054975"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK 105961"],"award-info":[{"award-number":["DK 105961"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK AA 20758"],"award-info":[{"award-number":["DK AA 20758"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1074\/jbc.m117.812925","type":"journal-article","created":{"date-parts":[[2017,10,18]],"date-time":"2017-10-18T16:20:16Z","timestamp":1508343616000},"page":"20989-20997","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["The lignan manassantin is a potent and specific inhibitor of mitochondrial complex I and bioenergetic activity in mammals"],"prefix":"10.1016","volume":"292","author":[{"given":"Yibao","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hae-Ki","family":"Min","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Unsong","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam M.","family":"Hawkridge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Mohsin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun","family":"Sanyal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward J.","family":"Lesnefsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianjun","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.M117.812925_bib1","doi-asserted-by":"crossref","first-page":"4947","DOI":"10.1016\/S0040-4039(01)99818-1","article-title":"Manassantins A\/B and saucerneol: novel biologically active lignoids from Saururus cernuus","volume":"24","author":"Rao","year":"1983","journal-title":"Tetrahedron Lett"},{"key":"10.1074\/jbc.M117.812925_bib2","series-title":"Dictionary of Korean Folk Medicine","first-page":"813","year":"1990"},{"key":"10.1074\/jbc.M117.812925_bib3","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/S0031-9422(03)00391-1","article-title":"Lignans from Saururus chinensis inhibiting the transcription factor NF-\u03baB","volume":"64","author":"Hwang","year":"2003","journal-title":"Phytochemistry"},{"key":"10.1074\/jbc.M117.812925_bib4","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1016\/S0006-2952(03)00553-7","article-title":"Suppression of RelA\/p65 transactivation activity by a lignoid manassantin isolated from Saururus chinensis","volume":"66","author":"Lee","year":"2003","journal-title":"Biochem. Pharmacol"},{"key":"10.1074\/jbc.M117.812925_bib5","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1254\/jphs.08299FP","article-title":"Inhibition of phenotypic and functional maturation of dendritic cells by manassantin a","volume":"109","author":"Kim","year":"2009","journal-title":"J. Pharmacol. Sci"},{"key":"10.1074\/jbc.M117.812925_bib6","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.1248\/bpb.b13-00146","article-title":"Manassantin B isolated from Saururus chinensis inhibits cyclooxygenase-2-dependent prostaglandin D2 generation by blocking Fyn-mediated nuclear factor-\u03baB and mitogen activated protein kinase pathways in bone marrow derived-mast cells","volume":"36","author":"Lu","year":"2013","journal-title":"Biol. Pharm. Bull"},{"key":"10.1074\/jbc.M117.812925_bib7","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S1016-8478(23)13205-5","article-title":"Inhibition of NF-IL6 activity by manassantin B, a dilignan isolated from Saururus chinensis, in phorbol myristate acetate-stimulated U937 promonocytic cells","volume":"20","author":"Son","year":"2005","journal-title":"Mol. Cells"},{"key":"10.1074\/jbc.M117.812925_bib8","doi-asserted-by":"crossref","first-page":"161","DOI":"10.4097\/kjae.2012.62.2.161","article-title":"Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1\u03b2 in RAW 264.7 cells","volume":"62","author":"Park","year":"2012","journal-title":"Korean J. Anesthesiol"},{"key":"10.1074\/jbc.M117.812925_bib9","doi-asserted-by":"crossref","first-page":"1769","DOI":"10.1248\/bpb.34.1769","article-title":"Manassantin A isolated from Saururus chinensis inhibits 5-lipoxygenase-dependent leukotriene C4 generation by blocking mitogen-activated protein kinase activation in mast cells","volume":"34","author":"Kim","year":"2011","journal-title":"Biol. Pharm. Bull"},{"key":"10.1074\/jbc.M117.812925_bib10","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1254\/jphs.10239SC","article-title":"Manassantin A and B from Saururus chinensis inhibit interleukin-6-induced signal transducer and activator of transcription 3 activation in Hep3B cells","volume":"115","author":"Chang","year":"2011","journal-title":"J. Pharmacol. Sci"},{"key":"10.1074\/jbc.M117.812925_bib11","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1021\/np030514m","article-title":"Molecular-targeted antitumor agents: the Saururus cernuus dineolignans manassantin B and 4-O-demethylmanassantin B are potent inhibitors of hypoxia-activated HIF-1","volume":"67","author":"Hodges","year":"2004","journal-title":"J. Nat. Prod"},{"key":"10.1074\/jbc.M117.812925_bib12","doi-asserted-by":"crossref","first-page":"7659","DOI":"10.1021\/acs.jmedchem.5b01220","article-title":"Synthesis and biological evaluation of manassantin analogues for hypoxia-inducible factor 1\u03b1 inhibition","volume":"58","author":"Kwon","year":"2015","journal-title":"J. Med. Chem"},{"key":"10.1074\/jbc.M117.812925_bib13","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1016\/j.bbrc.2005.05.191","article-title":"Saururus cernuus lignans\u2014potent small molecule inhibitors of hypoxia-inducible factor-1","volume":"333","author":"Hossain","year":"2005","journal-title":"Biochem. Biophys. Res. Commun"},{"key":"10.1074\/jbc.M117.812925_bib14","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.tibs.2008.08.002","article-title":"HIF-1 regulation: not so easy come, easy go","volume":"33","author":"Yee Koh","year":"2008","journal-title":"Trends Biochem. Sci"},{"key":"10.1074\/jbc.M117.812925_bib15","doi-asserted-by":"crossref","first-page":"2688","DOI":"10.1021\/acs.jproteome.6b00237","article-title":"Discovery of manassantin A protein targets using large-scale protein folding and stability measurements","volume":"15","author":"Geer Wallace","year":"2016","journal-title":"J. Proteome Res"},{"key":"10.1074\/jbc.M117.812925_bib16","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1002\/ptr.2791","article-title":"Manassantin A and B from Saururus chinensis inhibiting cellular melanin production","volume":"23","author":"Seo","year":"2009","journal-title":"Phytother. Res"},{"key":"10.1074\/jbc.M117.812925_bib17","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1055\/s-2003-818007","article-title":"Inhibitory effects of manassantin A and B isolated from the roots of Saururus chinensis on PMA-induced ICAM-1 expression","volume":"69","author":"Rho","year":"2003","journal-title":"Planta Med"},{"key":"10.1074\/jbc.M117.812925_bib18","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/BF02975136","article-title":"Manassantin A and B isolated from Saururus chinensis inhibit TNF-\u03b1-induced cell adhesion molecule expression of human umbilical vein endothelial cells","volume":"28","author":"Kwon","year":"2005","journal-title":"Arch. Pharm. Res"},{"key":"10.1074\/jbc.M117.812925_bib19","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1016\/j.bmcl.2004.04.023","article-title":"Human ACAT-1 and -2 inhibitory activities of saucerneol B, manassantin A and B isolated from Saururus chinensis","volume":"14","author":"Lee","year":"2004","journal-title":"Bioorg. Med. Chem. Lett"},{"key":"10.1074\/jbc.M117.812925_bib20","first-page":"517","article-title":"Neolignans from Saururus chinensis inhibit PC-3 prostate cancer cell growth via apoptosis and senescence-like mechanisms","volume":"16","author":"Song","year":"2005","journal-title":"Int. J. Mol. Med"},{"key":"10.1074\/jbc.M117.812925_bib21","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1021\/cb300495e","article-title":"Integrated compound profiling screens identify the mitochondrial electron transport chain as the molecular target of the natural products manassantin, sesquicillin, and arctigenin","volume":"8","author":"Lai","year":"2013","journal-title":"ACS Chem. Biol"},{"key":"10.1074\/jbc.M117.812925_bib22","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1136\/jmedgenet-2012-101159","article-title":"Complex I deficiency: clinical features, biochemistry and molecular genetics","volume":"49","author":"Fassone","year":"2012","journal-title":"J. Med. Genet"},{"key":"10.1074\/jbc.M117.812925_bib23","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/S0005-2728(98)00029-2","article-title":"Inhibitors of NADH-ubiquinone reductase: an overview","volume":"1364","author":"Degli Esposti","year":"1998","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M117.812925_bib24","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1016\/j.bbabio.2015.11.009","article-title":"Current topics on inhibitors of respiratory complex I","volume":"1857","author":"Murai","year":"2016","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/jbc.M117.812925_bib25","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1093\/toxsci\/kfw126","article-title":"Identification of the additional mitochondrial liabilities of 2-hydroxyflutamide when compared with its parent compound, flutamide in HepG2 cells","volume":"153","author":"Ball","year":"2016","journal-title":"Toxicol. Sci"},{"key":"10.1074\/jbc.M117.812925_bib26","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.tox.2015.03.001","article-title":"Sodium valproate induces mitochondrial respiration dysfunction in HepG2 in vitro cell model","volume":"331","author":"Komulainen","year":"2015","journal-title":"Toxicology"},{"key":"10.1074\/jbc.M117.812925_bib27","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1038\/cr.2011.55","article-title":"Mitochondrial H2O2 generated from electron transport chain complex I stimulates muscle differentiation","volume":"21","author":"Lee","year":"2011","journal-title":"Cell Res"},{"key":"10.1074\/jbc.M117.812925_bib28","doi-asserted-by":"crossref","first-page":"e12710","DOI":"10.14814\/phy2.12710","article-title":"Nanosecond pulsed platelet-rich plasma (nsPRP) improves mechanical and electrical cardiac function following myocardial reperfusion injury","volume":"4","author":"Hargrave","year":"2016","journal-title":"Physiol. Rep"},{"key":"10.1074\/jbc.M117.812925_bib29","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1096\/fj.10-167502","article-title":"Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration","volume":"25","author":"Strub","year":"2011","journal-title":"FASEB J"},{"key":"10.1074\/jbc.M117.812925_bib30","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/nrm3997","article-title":"A giant molecular proton pump: structure and mechanism of respiratory complex I","volume":"16","author":"Sazanov","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol"},{"key":"10.1074\/jbc.M117.812925_bib31","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1146\/annurev-cellbio-092910-154237","article-title":"Aerobic glycolysis: meeting the metabolic requirements of cell proliferation","volume":"27","author":"Lunt","year":"2011","journal-title":"Annu. Rev. Cell Dev. Biol"},{"key":"10.1074\/jbc.M117.812925_bib32","doi-asserted-by":"crossref","first-page":"6132","DOI":"10.1038\/onc.2016.150","article-title":"A causal link from ALK to hexokinase II overexpression and hyperactive glycolysis in EML4-ALK-positive lung cancer","volume":"35","author":"Ma","year":"2016","journal-title":"Oncogene"},{"key":"10.1074\/jbc.M117.812925_bib33","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.neo.2015.09.003","article-title":"Lysophosphatidic acid up-regulates hexokinase II and glycolysis to promote proliferation of ovarian cancer cells","volume":"17","author":"Mukherjee","year":"2015","journal-title":"Neoplasia"},{"key":"10.1074\/jbc.M117.812925_bib34","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.2174\/138955709788922610","article-title":"HIF-1\u03b1 modulates energy metabolism in cancer cells by inducing over-expression of specific glycolytic isoforms","volume":"9","author":"Mar\u00edn-Hern\u00e1ndez","year":"2009","journal-title":"Mini Rev. Med. Chem"},{"key":"10.1074\/jbc.M117.812925_bib35","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1038\/nchembio.2007.54","article-title":"Dorsomorphin inhibits BMP signals required for embryogenesis and iron metabolism","volume":"4","author":"Yu","year":"2008","journal-title":"Nat. Chem. Biol"},{"key":"10.1074\/jbc.M117.812925_bib36","doi-asserted-by":"crossref","first-page":"22162","DOI":"10.1016\/S0021-9258(17)31770-2","article-title":"Critical phosphorylation sites for acetyl-CoA carboxylase activity","volume":"269","author":"Ha","year":"1994","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.M117.812925_bib37","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1101\/gad.17420111","article-title":"AMP-activated protein kinase: an energy sensor that regulates all aspects of cell function","volume":"25","author":"Hardie","year":"2011","journal-title":"Genes Dev"},{"key":"10.1074\/jbc.M117.812925_bib38","series-title":"Drug-Induced Mitochondrial Dysfunction","first-page":"373","article-title":"Mitochondrial dysfunction assessed quantitatively in real time by measuring the extracellular flux of oxygen and protons","author":"Ferrick","year":"2008"},{"key":"10.1074\/jbc.M117.812925_bib39","doi-asserted-by":"crossref","DOI":"10.3791\/2511","article-title":"Bioenergetic profile experiment using C2C12 myoblast cells","author":"Nicholls","year":"2010","journal-title":"J. Vis. Exp"},{"key":"10.1074\/jbc.M117.812925_bib40","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1042\/bj1880451","article-title":"Effect of carnitine on mitochondrial oxidation of palmitoylearnitine","volume":"188","author":"Brass","year":"1980","journal-title":"Biochem. J"},{"key":"10.1074\/jbc.M117.812925_bib41","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1172\/JCI113066","article-title":"Deficiency of the reduced nicotinamide adenine dinucleotide dehydrogenase component of complex I of mitochondrial electron transport. Fatal infantile lactic acidosis and hypermetabolism with skeletal-cardiac myopathy and encephalopathy","volume":"80","author":"Hoppel","year":"1987","journal-title":"J. Clin. Investig"},{"key":"10.1074\/jbc.M117.812925_bib42","doi-asserted-by":"crossref","first-page":"3832","DOI":"10.18632\/oncotarget.6757","article-title":"Carnitine palmitoyltransferase 1A functions to repress FoxO transcription factors to allow cell cycle progression in ovarian cancer","volume":"7","author":"Shao","year":"2016","journal-title":"Oncotarget"},{"key":"10.1074\/jbc.M117.812925_bib43","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1182\/blood.V30.3.311.311","article-title":"A comparison of normal red cell ATP levels as measured by the firefly system and the hexokinase system","volume":"30","author":"Beutler","year":"1967","journal-title":"Blood"},{"key":"10.1074\/jbc.M117.812925_bib44","doi-asserted-by":"crossref","first-page":"11960","DOI":"10.1073\/pnas.220413597","article-title":"Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A","volume":"97","author":"Fang","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820327496?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820327496?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.M117.812925","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T21:22:35Z","timestamp":1719523355000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820327496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":44,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["S0021925820327496"],"URL":"https:\/\/doi.org\/10.1074\/jbc.m117.812925","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The lignan manassantin is a potent and specific inhibitor of mitochondrial complex I and bioenergetic activity in mammals","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.M117.812925","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}