{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:16:49Z","timestamp":1773422209320,"version":"3.50.1"},"reference-count":44,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T00:00:00Z","timestamp":1598227200000},"content-version":"vor","delay-in-days":570,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1074\/jbc.ra118.004511","type":"journal-article","created":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T02:45:16Z","timestamp":1543632316000},"page":"1478-1487","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":42,"title":["Structural progression of amyloid-\u03b2 Arctic mutant aggregation in cells revealed by multiparametric imaging"],"prefix":"10.1016","volume":"294","author":[{"given":"Meng","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajay","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire H.","family":"Michel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemens F.","family":"Kaminski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8570-125X","authenticated-orcid":false,"given":"Alan","family":"Tunnacliffe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1843-2202","authenticated-orcid":false,"given":"Gabriele S.","family":"Kaminski Schierle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.RA118.004511_bib1","doi-asserted-by":"crossref","first-page":"595","DOI":"10.15252\/emmm.201606210","article-title":"The amyloid hypothesis of Alzheimer's disease at 25 years","volume":"8","author":"Selkoe","year":"2016","journal-title":"EMBO Mol. Med"},{"key":"10.1074\/jbc.RA118.004511_bib2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3233\/JAD-2010-1221","article-title":"Alzheimer\u2019s disease and the amyloid-\u03b2 peptide","volume":"19","author":"Murphy","year":"2010","journal-title":"J. Alzheimers Dis"},{"key":"10.1074\/jbc.RA118.004511_bib3","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1016\/j.cell.2012.02.022","article-title":"The amyloid state of proteins in human diseases","volume":"148","author":"Eisenberg","year":"2012","journal-title":"Cell"},{"key":"10.1074\/jbc.RA118.004511_bib4","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/nrn2168","article-title":"Intracellular amyloid-\u03b2 in Alzheimer\u2019s disease","volume":"8","author":"LaFerla","year":"2007","journal-title":"Nat. Rev. Neurosci"},{"key":"10.1074\/jbc.RA118.004511_bib5","doi-asserted-by":"crossref","first-page":"a006270","DOI":"10.1101\/cshperspect.a006270","article-title":"Trafficking and proteolytic processing of APP. (2012)","volume":"2","author":"Haass","year":"2012","journal-title":"Cold Spring Harb. Perspect. Med"},{"key":"10.1074\/jbc.RA118.004511_bib6","doi-asserted-by":"crossref","first-page":"23243","DOI":"10.1074\/jbc.270.40.23243","article-title":"Regulated formation of Golgi secretory vesicles containing Alzheimer \u03b2-amyloid precursor protein","volume":"270","author":"Xu","year":"1995","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.RA118.004511_bib7","doi-asserted-by":"crossref","first-page":"2250","DOI":"10.1038\/ncomms3250","article-title":"Phosphatidylinositol-3-phosphate regulates sorting and processing of amyloid precursor protein through the endosomal system","volume":"4","author":"Morel","year":"2013","journal-title":"Nat. Commun"},{"key":"10.1074\/jbc.RA118.004511_bib8","doi-asserted-by":"crossref","first-page":"3339","DOI":"10.1242\/jcs.00643","article-title":"Demonstration by FRET of BACE interaction with the amyloid precursor protein at the cell surface and in early endosomes","volume":"116","author":"Kinoshita","year":"2003","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.RA118.004511_bib9","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0006-8993(92)90898-J","article-title":"Differential distribution of cellular forms of \u03b2-amyloid precursor protein in murine glial cell cultures","volume":"584","author":"Mizuguchi","year":"1992","journal-title":"Brain Res"},{"key":"10.1074\/jbc.RA118.004511_bib10","doi-asserted-by":"crossref","first-page":"9513","DOI":"10.1073\/pnas.90.20.9513","article-title":"Human neurons derived from a teratocarcinoma cell line express solely the 695-amino acid amyloid precursor protein and produce intracellular \u03b2-amyloid or A4 peptides","volume":"90","author":"Wertkin","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.RA118.004511_bib11","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/ng1718","article-title":"APP locus duplication causes autosomal dominant early-onset Alzheimer disease with cerebral amyloid angiopathy","volume":"38","author":"Rovelet-Lecrux","year":"2006","journal-title":"Nat. Genet"},{"key":"10.1074\/jbc.RA118.004511_bib12","doi-asserted-by":"crossref","first-page":"369","DOI":"10.3233\/JAD-132543","article-title":"Amyloid-\u03b2 peptides are generated in mitochondria-associated endoplasmic reticulum membranes","volume":"43","author":"Schreiner","year":"2015","journal-title":"J. Alzheimers Dis"},{"key":"10.1074\/jbc.RA118.004511_bib13","doi-asserted-by":"crossref","first-page":"10831","DOI":"10.1021\/bi001048s","article-title":"The oligomerization of amyloid \u03b2-protein begins intracellularly in cells derived from human brain","volume":"39","author":"Walsh","year":"2000","journal-title":"Biochemistry"},{"key":"10.1074\/jbc.RA118.004511_bib14","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.1523\/JNEUROSCI.5543-03.2004","article-title":"Dimeric amyloid \u03b2 protein rapidly accumulates in lipid rafts followed by apolipoprotein E and phosphorylated tau accumulation in the Tg2576 mouse model of Alzheimer\u2019s disease","volume":"24","author":"Kawarabayashi","year":"2004","journal-title":"J. Neurosci"},{"key":"10.1074\/jbc.RA118.004511_bib15","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1002\/jcb.20978","article-title":"Amyloid \u03b2 oligomerization is induced by brain lipid rafts","volume":"99","author":"Kim","year":"2006","journal-title":"J. Cell. Biochem"},{"key":"10.1074\/jbc.RA118.004511_bib16","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.chembiol.2014.03.014","article-title":"Direct observations of amyloid \u03b2 self-assembly in live cells provide insights into differences in the kinetics of A\u03b2(1\u201340) and A\u03b2(1\u201342) aggregation","volume":"21","author":"Esbj\u00f6rner","year":"2014","journal-title":"Chem. Biol"},{"key":"10.1074\/jbc.RA118.004511_bib17","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1074\/jbc.M113.515445","article-title":"Extracellular monomeric tau protein is sufficient to initiate the spread of tau protein pathology","volume":"289","author":"Michel","year":"2014","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.RA118.004511_bib18","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1038\/ng.440","article-title":"Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer's disease","volume":"41","author":"Harold","year":"2009","journal-title":"Nat. Genet"},{"key":"10.1074\/jbc.RA118.004511_bib19","doi-asserted-by":"crossref","first-page":"978","DOI":"10.1038\/nn.4025","article-title":"Central role for PICALM in amyloid-\u03b2 blood-brain barrier transcytosis and clearance","volume":"18","author":"Zhao","year":"2015","journal-title":"Nat. Neurosci"},{"key":"10.1074\/jbc.RA118.004511_bib20","doi-asserted-by":"crossref","first-page":"184","DOI":"10.2353\/ajpath.2006.050593","article-title":"A dynamic relationship between intracellular and extracellular pools of A\u03b242","volume":"168","author":"Oddo","year":"2006","journal-title":"Am. J. Pathol"},{"key":"10.1074\/jbc.RA118.004511_bib21","doi-asserted-by":"crossref","first-page":"12893","DOI":"10.1523\/JNEUROSCI.1201-14.2014","article-title":"Intracellular accumulation of amyloid-\u03b2 (A\u03b242) protein plays a major role in A\u03b242-induced alterations of glutamatergic synaptic transmission and plasticity","volume":"34","author":"Ripoli","year":"2014","journal-title":"J. Neurosci"},{"key":"10.1074\/jbc.RA118.004511_bib22","doi-asserted-by":"crossref","first-page":"e0168157","DOI":"10.1371\/journal.pone.0168157","article-title":"Metabolic characterization of intact cells reveals intracellular amyloid \u03b3 but not its precursor protein to reduce mitochondrial respiration","volume":"11","author":"Schaefer","year":"2016","journal-title":"PLoS One"},{"key":"10.1074\/jbc.RA118.004511_bib23","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1046\/j.1365-2559.2001.01082.x","article-title":"Evidence that neurones accumulating amyloid can undergo lysis to form amyloid plaques in Alzheimer's disease","volume":"38","author":"D'Andrea","year":"2001","journal-title":"Histopathology"},{"key":"10.1074\/jbc.RA118.004511_bib24","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.nbd.2014.07.011","article-title":"Intracellular amyloid and the neuronal origin of Alzheimer neuritic plaques","volume":"71","author":"Pensalfini","year":"2014","journal-title":"Neurobiol. Dis"},{"key":"10.1074\/jbc.RA118.004511_bib25","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1038\/nn0901-887","article-title":"The \u201cArctic\u201d APP mutation (E693G) causes Alzheimer\u2019s disease by enhanced A\u03b242 protofibril formation","volume":"4","author":"Nilsberth","year":"2001","journal-title":"Nat. Neurosci"},{"key":"10.1074\/jbc.RA118.004511_bib26","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.neurobiolaging.2004.12.007","article-title":"The Arctic Alzheimer mutation facilitates early intraneuronal A\u03b242 aggregation and senile plaque formation in transgenic mice","volume":"27","author":"Lord","year":"2006","journal-title":"Neurobiol. Aging"},{"key":"10.1074\/jbc.RA118.004511_bib27","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1016\/j.neurobiolaging.2006.06.019","article-title":"Intracellular A\u03b2 and cognitive deficits precede \u03b2-amyloid deposition in transgenic ArcA\u03b2 mice","volume":"28","author":"Knobloch","year":"2007","journal-title":"Neurobiol. Aging"},{"key":"10.1074\/jbc.RA118.004511_bib28","first-page":"e53988","article-title":"A guide to structured illumination TIRF microscopy at high speed with multiple colors","author":"Young","year":"2016","journal-title":"J. Vis. Exp"},{"key":"10.1074\/jbc.RA118.004511_bib29","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1021\/nl4041093","article-title":"Direct observation of heterogeneous amyloid fibril growth kinetics via two-color super-resolution microscopy","volume":"14","author":"Pinotsi","year":"2014","journal-title":"Nano Lett"},{"key":"10.1074\/jbc.RA118.004511_bib30","doi-asserted-by":"crossref","first-page":"12902","DOI":"10.1021\/ja201651w","article-title":"In situ measurements of the formation and morphology of intracellular \u03b2-amyloid fibrils by super-resolution fluorescence imaging","volume":"133","author":"Kaminski Schierle","year":"2011","journal-title":"J. Am. Chem. Soc"},{"key":"10.1074\/jbc.RA118.004511_bib31","doi-asserted-by":"crossref","first-page":"e70687","DOI":"10.1371\/journal.pone.0070687","article-title":"Rapid global fitting of large fluorescence lifetime imaging microscopy datasets","volume":"8","author":"Warren","year":"2013","journal-title":"PLoS One"},{"key":"10.1074\/jbc.RA118.004511_bib32","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1021\/acs.nanolett.6b03686","article-title":"Fluorescence self-quenching from reporter dyes informs on the structural properties of amyloid clusters formed in vitro and in cells","volume":"17","author":"Chen","year":"2017","journal-title":"Nano Lett"},{"key":"10.1074\/jbc.RA118.004511_bib33","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1002\/bit.25606","article-title":"Expression-level dependent perturbation of cell proteostasis and nuclear morphology by aggregation-prone polyglutamine proteins","volume":"112","author":"Lu","year":"2015","journal-title":"Biotechnol. Bioeng"},{"key":"10.1074\/jbc.RA118.004511_bib34","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1083\/jcb.143.7.1883","article-title":"Aggresomes: a cellular response to misfolded proteins","volume":"143","author":"Johnston","year":"1998","journal-title":"J. Cell Biol"},{"key":"10.1074\/jbc.RA118.004511_bib35","doi-asserted-by":"crossref","first-page":"27986","DOI":"10.1074\/jbc.M115.676437","article-title":"Long term aggresome accumulation leads to DNA damage, p53-dependent cell cycle arrest, and steric interference in mitosis","volume":"290","author":"Lu","year":"2015","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.RA118.004511_bib36","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jsb.2012.06.010","article-title":"Aggregation and fibril morphology of the Arctic mutation of Alzheimer\u2019s A\u03b242 peptide by CD, TEM, STEM and in situ AFM","volume":"180","author":"Norlin","year":"2012","journal-title":"J. Struct. Biol"},{"key":"10.1074\/jbc.RA118.004511_bib37","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1007\/s00894-009-0611-1","article-title":"Effect of pathogenic mutations on the structure and dynamics of Alzheimer\u2019s A\u03b242-amyloid oligomers","volume":"16","author":"Kassler","year":"2010","journal-title":"J. Mol. Model"},{"key":"10.1074\/jbc.RA118.004511_bib38","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.bpj.2009.04.017","article-title":"Influence of residue 22 on the folding, aggregation profile, and toxicity of the Alzheimer\u2019s amyloid \u03b2 peptide","volume":"97","author":"Per\u00e1lvarez-Mar\u00edn","year":"2009","journal-title":"Biophys. J"},{"key":"10.1074\/jbc.RA118.004511_bib39","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1126\/science.2237415","article-title":"A thermodynamic scale for the helix-forming tendencies of the commonly occurring amino acids","volume":"250","author":"O'Neil","year":"1990","journal-title":"Science"},{"key":"10.1074\/jbc.RA118.004511_bib40","doi-asserted-by":"crossref","first-page":"L47","DOI":"10.1016\/j.bpj.2012.11.009","article-title":"Effects of familial mutations on the monomer structure of A\u03b242","volume":"103","author":"Lin","year":"2012","journal-title":"Biophys. J"},{"key":"10.1074\/jbc.RA118.004511_bib41","doi-asserted-by":"crossref","first-page":"2689","DOI":"10.1093\/hmg\/11.22.2689","article-title":"Aggregate formation inhibits proteasomal degradation of polyglutamine proteins","volume":"11","author":"Verhoef","year":"2002","journal-title":"Hum. Mol. Genet"},{"key":"10.1074\/jbc.RA118.004511_bib42","doi-asserted-by":"crossref","first-page":"15194","DOI":"10.1074\/jbc.M113.457408","article-title":"Lewy body-like \u03b1-synuclein aggregates resist degradation and impair macroautophagy","volume":"288","author":"Tanik","year":"2013","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.RA118.004511_bib43","doi-asserted-by":"crossref","first-page":"9758","DOI":"10.1073\/pnas.1218402110","article-title":"Proliferation of amyloid-\u03b242 aggregates occurs through a secondary nucleation mechanism","volume":"110","author":"Cohen","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.RA118.004511_bib44","doi-asserted-by":"crossref","first-page":"e1039","DOI":"10.7554\/eLife.10396","article-title":"Axial contraction and short-range compaction of chromatin synergistically promote mitotic chromosome condensation","volume":"4","author":"Kruitwagen","year":"2015","journal-title":"Elife"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820364589?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820364589?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.RA118.004511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,25]],"date-time":"2022-09-25T11:08:08Z","timestamp":1664104088000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820364589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["S0021925820364589"],"URL":"https:\/\/doi.org\/10.1074\/jbc.ra118.004511","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural progression of amyloid-\u03b2 Arctic mutant aggregation in cells revealed by multiparametric imaging","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.RA118.004511","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Lu et al.","name":"copyright","label":"Copyright"}]}}