{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T13:11:43Z","timestamp":1775740303693,"version":"3.50.1"},"reference-count":56,"publisher":"Elsevier BV","issue":"49","license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T00:00:00Z","timestamp":1597276800000},"content-version":"vor","delay-in-days":256,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004358","name":"Samsung","doi-asserted-by":"publisher","award":["SRFC-MA1601\u201306"],"award-info":[{"award-number":["SRFC-MA1601\u201306"]}],"id":[{"id":"10.13039\/100004358","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003715","name":"Korea Research Institute of Bioscience and Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1074\/jbc.ra119.009061","type":"journal-article","created":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T22:01:40Z","timestamp":1569880900000},"page":"18547-18556","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mass cytometry\u2013based single-cell analysis of human stem cell reprogramming uncovers differential regulation of specific pluripotency markers"],"prefix":"10.1016","volume":"294","author":[{"given":"Ilkyun","family":"Im","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye Seul","family":"Son","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang Bo","family":"Jung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Insoo","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boon-Eng","family":"Teh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung-Bok","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-Young","family":"Son","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3227-958X","authenticated-orcid":false,"given":"Janghwan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.RA119.009061_bib1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors","volume":"126","author":"Takahashi","year":"2006","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.cell.2007.11.019","article-title":"Induction of pluripotent stem cells from adult human fibroblasts by defined factors","volume":"131","author":"Takahashi","year":"2007","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib3","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1126\/science.1151526","article-title":"Induced pluripotent stem cell lines derived from human somatic cells","volume":"318","author":"Yu","year":"2007","journal-title":"Science"},{"key":"10.1074\/jbc.RA119.009061_bib4","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nature06534","article-title":"Reprogramming of human somatic cells to pluripotency with defined factors","volume":"451","author":"Park","year":"2008","journal-title":"Nature"},{"key":"10.1074\/jbc.RA119.009061_bib5","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/nrg3473","article-title":"Mechanisms and models of somatic cell reprogramming","volume":"14","author":"Buganim","year":"2013","journal-title":"Nat. Rev. Genet"},{"key":"10.1074\/jbc.RA119.009061_bib6","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.stem.2012.11.008","article-title":"A late transition in somatic cell reprogramming requires regulators distinct from the pluripotency network","volume":"11","author":"Golipour","year":"2012","journal-title":"Cell Stem Cell"},{"key":"10.1074\/jbc.RA119.009061_bib7","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/j.cell.2008.01.015","article-title":"Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming","volume":"132","author":"Jaenisch","year":"2008","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib8","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.stem.2010.04.014","article-title":"A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts","volume":"7","author":"Li","year":"2010","journal-title":"Cell Stem Cell"},{"key":"10.1074\/jbc.RA119.009061_bib9","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1101\/sqb.2010.75.002","article-title":"Efficiencies and mechanisms of nuclear reprogramming","volume":"75","author":"Pasque","year":"2010","journal-title":"Cold Spring Harb. Symp. Quant. Biol"},{"key":"10.1074\/jbc.RA119.009061_bib10","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1016\/j.cell.2012.11.039","article-title":"A molecular roadmap of reprogramming somatic cells into iPS cells","volume":"151","author":"Polo","year":"2012","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib11","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.stem.2010.04.015","article-title":"Functional genomics reveals a BMP-driven mesenchymal-to-epithelial transition in the initiation of somatic cell reprogramming","volume":"7","author":"Samavarchi-Tehrani","year":"2010","journal-title":"Cell Stem Cell"},{"key":"10.1074\/jbc.RA119.009061_bib12","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1101\/gad.1963910","article-title":"Induced pluripotency: history, mechanisms, and applications","volume":"24","author":"Stadtfeld","year":"2010","journal-title":"Genes Dev"},{"key":"10.1074\/jbc.RA119.009061_bib13","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.molcel.2012.09.008","article-title":"Molecular roadblocks for cellular reprogramming","volume":"47","author":"Vierbuchen","year":"2012","journal-title":"Mol. Cell"},{"key":"10.1074\/jbc.RA119.009061_bib14","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1016\/j.cell.2015.11.017","article-title":"A XEN-like state bridges somatic cells to pluripotency during chemical reprogramming","volume":"163","author":"Zhao","year":"2015","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib15","doi-asserted-by":"crossref","first-page":"6813","DOI":"10.1021\/ac901049w","article-title":"Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry","volume":"81","author":"Bandura","year":"2009","journal-title":"Anal. Chem"},{"key":"10.1074\/jbc.RA119.009061_bib16","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.cell.2014.04.005","article-title":"Single-cell trajectory detection uncovers progression and regulatory coordination in human B cell development","volume":"157","author":"Bendall","year":"2014","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib17","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1016\/j.jaci.2015.09.013","article-title":"Expansion of inflammatory innate lymphoid cells in patients with common variable immune deficiency","volume":"137","author":"Cols","year":"2016","journal-title":"J. Allergy Clin. Immunol"},{"key":"10.1074\/jbc.RA119.009061_bib18","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.cell.2015.05.047","article-title":"Data-driven phenotypic dissection of AML reveals progenitor-like cells that correlate with prognosis","volume":"162","author":"Levine","year":"2015","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib19","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/nri.2016.56","article-title":"Computational flow cytometry: helping to make sense of high-dimensional immunology data","volume":"16","author":"Saeys","year":"2016","journal-title":"Nat. Rev. Immunol"},{"key":"10.1074\/jbc.RA119.009061_bib20","doi-asserted-by":"crossref","first-page":"297ra115","DOI":"10.1126\/scitranslmed.aac5722","article-title":"Human NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility","volume":"7","author":"Strauss-Albee","year":"2015","journal-title":"Sci. Transl. Med"},{"key":"10.1074\/jbc.RA119.009061_bib21","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1002\/cyto.a.22075","article-title":"Single-cell mass cytometry adapted to measurements of the cell cycle","volume":"81","author":"Behbehani","year":"2012","journal-title":"Cytometry A"},{"key":"10.1074\/jbc.RA119.009061_bib22","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.2869","article-title":"Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry","volume":"11","author":"Giesen","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1074\/jbc.RA119.009061_bib23","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1038\/nature14274","article-title":"Early reprogramming regulators identified by prospective isolation and mass cytometry","volume":"521","author":"Lujan","year":"2015","journal-title":"Nature"},{"key":"10.1074\/jbc.RA119.009061_bib24","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.stem.2015.01.015","article-title":"A continuous molecular roadmap to iPSC reprogramming through progression analysis of single-cell mass cytometry","volume":"16","author":"Zunder","year":"2015","journal-title":"Cell Stem Cell"},{"key":"10.1074\/jbc.RA119.009061_bib25","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1038\/nbt.1991","article-title":"Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE","volume":"29","author":"Qiu","year":"2011","journal-title":"Nat. Biotechnol"},{"key":"10.1074\/jbc.RA119.009061_bib26","first-page":"2579","article-title":"Visualizing data using t-SNE","volume":"9","author":"Laurens van der Maaten","year":"2008","journal-title":"Journal of Machine Learning Research"},{"key":"10.1074\/jbc.RA119.009061_bib27","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/nbt.2594","article-title":"viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia","volume":"31","author":"Amir","year":"2013","journal-title":"Nat. Biotechnol"},{"key":"10.1074\/jbc.RA119.009061_bib28","doi-asserted-by":"crossref","first-page":"e1005112","DOI":"10.1371\/journal.pcbi.1005112","article-title":"Cytofkit: a Bioconductor package for an integrated mass cytometry data analysis pipeline","volume":"12","author":"Chen","year":"2016","journal-title":"PLoS Comput. Biol"},{"key":"10.1074\/jbc.RA119.009061_bib29","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1038\/ni.3006","article-title":"High-dimensional analysis of the murine myeloid cell system","volume":"15","author":"Becher","year":"2014","journal-title":"Nat. Immunol"},{"key":"10.1074\/jbc.RA119.009061_bib30","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1126\/science.290.5500.2319","article-title":"A global geometric framework for nonlinear dimensionality reduction","volume":"290","author":"Tenenbaum","year":"2000","journal-title":"Science"},{"key":"10.1074\/jbc.RA119.009061_bib31","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1093\/bioinformatics\/btv325","article-title":"Diffusion maps for high-dimensional single-cell analysis of differentiation data","volume":"31","author":"Haghverdi","year":"2015","journal-title":"Bioinformatics"},{"key":"10.1074\/jbc.RA119.009061_bib32","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1042\/BJ20121627","article-title":"The cell cycle and pluripotency","volume":"451","author":"Hindley","year":"2013","journal-title":"Biochem. J"},{"key":"10.1074\/jbc.RA119.009061_bib33","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/ncb3474","article-title":"G1 cyclins link proliferation, pluripotency and differentiation of embryonic stem cells","volume":"19","author":"Liu","year":"2017","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/jbc.RA119.009061_bib34","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/978-1-4939-0888-2_10","article-title":"Cell cycle regulation during viral infection","volume":"1170","author":"Bagga","year":"2014","journal-title":"Methods Mol. Biol"},{"key":"10.1074\/jbc.RA119.009061_bib35","first-page":"197","article-title":"The cell cycle and virus infection","volume":"296","author":"Emmett","year":"2005","journal-title":"Methods Mol. Biol"},{"key":"10.1074\/jbc.RA119.009061_bib36","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1002\/jcb.20358","article-title":"Effects of cell cycle status on early events in retroviral replication","volume":"94","author":"Katz","year":"2005","journal-title":"J. Cell Biochem"},{"key":"10.1074\/jbc.RA119.009061_bib37","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1038\/nprot.2009.231","article-title":"Generation of mouse-induced pluripotent stem cells with plasmid vectors","volume":"5","author":"Okita","year":"2010","journal-title":"Nat. Protoc"},{"key":"10.1074\/jbc.RA119.009061_bib38","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.gpb.2013.09.008","article-title":"Integration-free methods for generating induced pluripotent stem cells","volume":"11","author":"Zhou","year":"2013","journal-title":"Genomics Proteomics Bioinformatics"},{"key":"10.1074\/jbc.RA119.009061_bib39","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1080\/19768354.2018.1451367","article-title":"Optimization of episomal reprogramming for generation of human induced pluripotent stem cells from fibroblasts","volume":"22","author":"Bang","year":"2018","journal-title":"Anim. Cells Syst. (Seoul)"},{"key":"10.1074\/jbc.RA119.009061_bib40","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1126\/science.1172482","article-title":"Human induced pluripotent stem cells free of vector and transgene sequences","volume":"324","author":"Yu","year":"2009","journal-title":"Science"},{"key":"10.1074\/jbc.RA119.009061_bib41","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1038\/ncb2164","article-title":"Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy","volume":"13","author":"Efe","year":"2011","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/jbc.RA119.009061_bib42","doi-asserted-by":"crossref","first-page":"7838","DOI":"10.1073\/pnas.1103113108","article-title":"Direct reprogramming of mouse fibroblasts to neural progenitors","volume":"108","author":"Kim","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.RA119.009061_bib43","first-page":"1","article-title":"Accelerating t-SNE using tree-based algorithms","volume":"15","author":"Maaten","year":"2014","journal-title":"Journal of Machine Learning Research"},{"key":"10.1074\/jbc.RA119.009061_bib44","doi-asserted-by":"crossref","first-page":"3943","DOI":"10.1038\/onc.2011.105","article-title":"Phosphorylation of histone H3 serine 28 modulates RNA polymerase III-dependent transcription","volume":"30","author":"Zhang","year":"2011","journal-title":"Oncogene"},{"key":"10.1074\/jbc.RA119.009061_bib45","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1038\/nbt.1580","article-title":"Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells","volume":"27","author":"Chan","year":"2009","journal-title":"Nat. Biotechnol"},{"key":"10.1074\/jbc.RA119.009061_bib46","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.cell.2015.07.001","article-title":"Deterministic Restriction on pluripotent state dissolution by cell-cycle pathways","volume":"162","author":"Gonzales","year":"2015","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib47","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1038\/sj.onc.1204326","article-title":"Histone H3 phosphorylation and cell division","volume":"20","author":"Hans","year":"2001","journal-title":"Oncogene"},{"key":"10.1074\/jbc.RA119.009061_bib48","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1002\/stem.1315","article-title":"An Oct4-pRb axis, controlled by MiR-335, integrates stem cell self-renewal and cell cycle control","volume":"31","author":"Schoeftner","year":"2013","journal-title":"Stem Cells"},{"key":"10.1074\/jbc.RA119.009061_bib49","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1089\/hyb.1984.3.347","article-title":"Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells","volume":"3","author":"Andrews","year":"1984","journal-title":"Hybridoma"},{"key":"10.1074\/jbc.RA119.009061_bib50","first-page":"4715","article-title":"The human embryonal carcinoma marker antigen TRA-1\u201360 is a sialylated keratan sulfate proteoglycan","volume":"59","author":"Badcock","year":"1999","journal-title":"Cancer Res"},{"key":"10.1074\/jbc.RA119.009061_bib51","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1634\/stemcells.2005-0597","article-title":"The TRA-1\u201360 and TRA-1\u201381 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma","volume":"25","author":"Schopperle","year":"2007","journal-title":"Stem Cells"},{"key":"10.1074\/jbc.RA119.009061_bib52","doi-asserted-by":"crossref","first-page":"8715","DOI":"10.1038\/ncomms9715","article-title":"Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids","volume":"6","author":"Freedman","year":"2015","journal-title":"Nat. Commun"},{"key":"10.1074\/jbc.RA119.009061_bib53","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1634\/stemcells.2006-0232","article-title":"The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency","volume":"25","author":"Brimble","year":"2007","journal-title":"Stem Cells"},{"key":"10.1074\/jbc.RA119.009061_bib54","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.cell.2013.08.031","article-title":"The cell-cycle state of stem cells determines cell fate propensity","volume":"155","author":"Pauklin","year":"2013","journal-title":"Cell"},{"key":"10.1074\/jbc.RA119.009061_bib55","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1111\/nan.12396","article-title":"Distinctive genomic signature of neural and intestinal organoids from familial Parkinson's disease patient-derived induced pluripotent stem cells","volume":"43","author":"Son","year":"2017","journal-title":"Neuropathol. Appl. Neurobiol"},{"key":"10.1074\/jbc.RA119.009061_bib56","doi-asserted-by":"crossref","DOI":"10.1002\/0471142956.cy1017s53","article-title":"Web-based analysis and publication of flow cytometry experiments","author":"Kotecha","year":"2010","journal-title":"Curr. Protoc. Cytom"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820304105?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925820304105?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.RA119.009061","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,18]],"date-time":"2021-12-18T12:54:11Z","timestamp":1639832051000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925820304105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12]]},"references-count":56,"journal-issue":{"issue":"49","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["S0021925820304105"],"URL":"https:\/\/doi.org\/10.1074\/jbc.ra119.009061","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2019,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mass cytometry\u2013based single-cell analysis of human stem cell reprogramming uncovers differential regulation of specific pluripotency markers","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.RA119.009061","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Im et al.","name":"copyright","label":"Copyright"}]}}