{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T02:58:52Z","timestamp":1771901932822,"version":"3.50.1"},"reference-count":36,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2020,3,1]],"date-time":"2020-03-01T00:00:00Z","timestamp":1583020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"vor","delay-in-days":276,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["MU 1477\/10\u20131"],"award-info":[{"award-number":["MU 1477\/10\u20131"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["MA 1289\/8\u20131"],"award-info":[{"award-number":["MA 1289\/8\u20131"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EI 423\/4\u20131"],"award-info":[{"award-number":["EI 423\/4\u20131"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2020,3]]},"DOI":"10.1074\/jbc.ra119.011546","type":"journal-article","created":{"date-parts":[[2020,1,25]],"date-time":"2020-01-25T00:44:15Z","timestamp":1579913055000},"page":"3189-3201","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":32,"title":["High-resolution crystal structures of two prototypical \u03b2- and \u03b3-herpesviral nuclear egress complexes unravel the determinants of subfamily specificity"],"prefix":"10.1016","volume":"295","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0519-8928","authenticated-orcid":false,"given":"Yves A.","family":"Muller","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigrun","family":"H\u00e4ge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sewar","family":"Alkhashrom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"H\u00f6llriegl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Weigert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Dolles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerstin","family":"Hof","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sascha A.","family":"Walzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9282-7459","authenticated-orcid":false,"given":"Claudia","family":"Egerer-Sieber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus","family":"Conrad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Holst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josephine","family":"L\u00f6sing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Sonntag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heinrich","family":"Sticht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jutta","family":"Eichler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Marschall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.RA119.011546_bib1","series-title":"Fields Virology","first-page":"1960","article-title":"Cytomegaloviruses","author":"Mocarski","year":"2013"},{"key":"10.1074\/jbc.RA119.011546_bib2","series-title":"Clinical Virology","first-page":"481","article-title":"Cytomegalovirus","author":"Griffiths","year":"2017"},{"key":"10.1074\/jbc.RA119.011546_bib3","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1741-4520.2009.00222.x","article-title":"Effects of cytomegalovirus infection on embryogenesis and brain development","volume":"49","author":"Tsutsui","year":"2009","journal-title":"Congenit. Anom. (Kyoto)"},{"key":"10.1074\/jbc.RA119.011546_bib4","doi-asserted-by":"crossref","first-page":"e18","DOI":"10.3390\/pathogens5010018","article-title":"The role of gammaherpesviruses in cancer pathogenesis","volume":"5","author":"Jha","year":"2016","journal-title":"Pathogens"},{"key":"10.1074\/jbc.RA119.011546_bib5","doi-asserted-by":"crossref","first-page":"24004","DOI":"10.1074\/jbc.M111.331207","article-title":"Specific residues of a conserved domain in the N terminus of the human cytomegalovirus pUL50 protein determine its intranuclear interaction with pUL53","volume":"287","author":"Milbradt","year":"2012","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.RA119.011546_bib6","doi-asserted-by":"crossref","first-page":"13979","DOI":"10.1074\/jbc.M109.063628","article-title":"Novel mode of phosphorylation-triggered reorganization of the nuclear lamina during nuclear egress of human cytomegalovirus","volume":"285","author":"Milbradt","year":"2010","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.RA119.011546_bib7","doi-asserted-by":"crossref","first-page":"2937","DOI":"10.15252\/embj.201592651","article-title":"Unexpected features and mechanism of heterodimer formation of a herpesvirus nuclear egress complex","volume":"34","author":"Lye","year":"2015","journal-title":"EMBO J"},{"key":"10.1074\/jbc.RA119.011546_bib8","doi-asserted-by":"crossref","first-page":"27452","DOI":"10.1074\/jbc.C115.686527","article-title":"Crystal structure of the human cytomegalovirus pUL50-pUL53 core nuclear egress complex provides insight into a unique assembly scaffold for virus-host protein interactions","volume":"290","author":"Walzer","year":"2015","journal-title":"J. Biol. Chem"},{"key":"10.1074\/jbc.RA119.011546_bib9","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.15252\/embj.201592359","article-title":"Structural basis of membrane budding by the nuclear egress complex of herpesviruses","volume":"34","author":"Bigalke","year":"2015","journal-title":"EMBO J"},{"key":"10.1074\/jbc.RA119.011546_bib10","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1016\/j.celrep.2015.11.008","article-title":"Crystal structure of the herpesvirus nuclear egress complex provides insights into inner nuclear membrane remodeling","volume":"13","author":"Zeev-Ben-Mordehai","year":"2015","journal-title":"Cell Rep"},{"key":"10.1074\/jbc.RA119.011546_bib11","doi-asserted-by":"crossref","first-page":"e46","DOI":"10.3390\/cells6040046","article-title":"Venture from the interior-herpesvirus pUL31 escorts capsids from nucleoplasmic replication compartments to sites of primary envelopment at the inner nuclear membrane","volume":"6","author":"Bailer","year":"2017","journal-title":"Cells"},{"key":"10.1074\/jbc.RA119.011546_bib12","doi-asserted-by":"crossref","first-page":"9150","DOI":"10.1128\/JVI.02530-14","article-title":"The great (nuclear) escape: new insights into the role of the nuclear egress complex of herpesviruses","volume":"89","author":"Bigalke","year":"2015","journal-title":"J. Virol"},{"key":"10.1074\/jbc.RA119.011546_bib13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/bs.aivir.2015.10.002","article-title":"Nuclear egress of herpesviruses: the prototypic vesicular nucleocytoplasmic transport","volume":"94","author":"Hellberg","year":"2016","journal-title":"Adv. Virus Res"},{"key":"10.1074\/jbc.RA119.011546_bib14","doi-asserted-by":"crossref","DOI":"10.1002\/rmv.1934","article-title":"The human cytomegalovirus nuclear egress complex unites multiple functions: recruitment of effectors, nuclear envelope rearrangement, and docking to nuclear capsids","volume":"27","author":"Marschall","year":"2017","journal-title":"Rev. Med. Virol"},{"key":"10.1074\/jbc.RA119.011546_bib15","doi-asserted-by":"crossref","first-page":"W351","DOI":"10.1093\/nar\/gkw357","article-title":"Dali server update","volume":"44","author":"Holm","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/jbc.RA119.011546_bib16","doi-asserted-by":"crossref","first-page":"9010","DOI":"10.1073\/pnas.1511140112","article-title":"Structure of a herpesvirus nuclear egress complex subunit reveals an interaction groove that is essential for viral replication","volume":"112","author":"Leigh","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.RA119.011546_bib17","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1128\/JVI.02441-08","article-title":"Biochemical, biophysical, and mutational analyses of subunit interactions of the human cytomegalovirus nuclear egress complex","volume":"83","author":"Sam","year":"2009","journal-title":"J. Virol"},{"key":"10.1074\/jbc.RA119.011546_bib18","doi-asserted-by":"crossref","DOI":"10.3390\/ijms19102908","article-title":"Conformational dynamics of herpesviral NEC proteins in different oligomerization states","volume":"19","author":"Diewald","year":"2018","journal-title":"Int. J. Mol. Sci"},{"key":"10.1074\/jbc.RA119.011546_bib19","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1006\/jmbi.1993.1611","article-title":"Comparison of a structural and a functional epitope","volume":"234","author":"Cunningham","year":"1993","journal-title":"J. Mol. Biol"},{"key":"10.1074\/jbc.RA119.011546_bib20","doi-asserted-by":"crossref","first-page":"D158","DOI":"10.1093\/nar\/gkw1099","article-title":"UniProt: the universal protein knowledgebase","volume":"45","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/jbc.RA119.011546_bib21","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1107\/S0909049512006395","article-title":"Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin","volume":"19","author":"Mueller","year":"2012","journal-title":"J. Synchrotron Radiat"},{"key":"10.1074\/jbc.RA119.011546_bib22","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1107\/S0021889893005588","article-title":"Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants","volume":"26","author":"Kabsch","year":"1993","journal-title":"J. Appl. Crystallogr"},{"key":"10.1074\/jbc.RA119.011546_bib23","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr"},{"key":"10.1074\/jbc.RA119.011546_bib24","first-page":"D271","article-title":"The RCSB Protein Data Bank: integrative view of protein, gene and 3D structural information","volume":"45","author":"Rose","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/jbc.RA119.011546_bib25","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1107\/S0907444910007493","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallogr. D Biol. Crystallogr"},{"key":"10.1074\/jbc.RA119.011546_bib26","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1126\/science.1218231","article-title":"Linking crystallographic model and data quality","volume":"336","author":"Karplus","year":"2012","journal-title":"Science"},{"key":"10.1074\/jbc.RA119.011546_bib27","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1107\/S0907444910045749","article-title":"Overview of the CCP4 suite and current developments","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Crystallogr. D Biol. Crystallogr"},{"key":"10.1074\/jbc.RA119.011546_bib28","series-title":"The PyMOL Molecular Graphics System","author":"DeLano","year":"2003"},{"key":"10.1074\/jbc.RA119.011546_bib29","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1107\/S1600576715006287","article-title":"Online_DPI: a web server to calculate the diffraction precision index for a protein structure","volume":"48","author":"Kumar","year":"2015","journal-title":"J. Appl. Crystallogr"},{"key":"10.1074\/jbc.RA119.011546_bib30","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1099\/vir.0.82393-0","article-title":"Mapping of a self-interaction domain of the cytomegalovirus protein kinase pUL97","volume":"88","author":"Schregel","year":"2007","journal-title":"J. Gen. Virol"},{"key":"10.1074\/jbc.RA119.011546_bib31","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1099\/vir.0.82924-0","article-title":"Cytomegaloviral proteins pUL50 and pUL53 are associated with the nuclear lamina and interact with cellular protein kinase C","volume":"88","author":"Milbradt","year":"2007","journal-title":"J. Gen. Virol"},{"key":"10.1074\/jbc.RA119.011546_bib32","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1099\/jgv.0.000495","article-title":"Cytomegalovirus pUL50 is the multi-interacting determinant of the core nuclear egress complex (NEC) that recruits cellular accessory NEC components","volume":"97","author":"Sonntag","year":"2016","journal-title":"J. Gen. Virol"},{"key":"10.1074\/jbc.RA119.011546_bib33","doi-asserted-by":"crossref","first-page":"W382","DOI":"10.1093\/nar\/gki387","article-title":"The FoldX web server: an online force field","volume":"33","author":"Schymkowitz","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/jbc.RA119.011546_bib34","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1002\/cbic.201402545","article-title":"Enhancement and induction of HIV-1 infection through an assembled peptide derived from the CD4 binding site of gp120","volume":"16","author":"Gro\u00df","year":"2015","journal-title":"ChemBioChem"},{"key":"10.1074\/jbc.RA119.011546_bib35","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/pro.3330","article-title":"MolProbity: more and better reference data for improved all-atom structure validation","volume":"27","author":"Williams","year":"2018","journal-title":"Protein Sci"},{"key":"10.1074\/jbc.RA119.011546_bib36","author":"Tickle","year":"2018"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925817495196?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925817495196?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.RA119.011546","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,25]],"date-time":"2022-09-25T10:37:49Z","timestamp":1664102269000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925817495196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3]]},"references-count":36,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,3]]}},"alternative-id":["S0021925817495196"],"URL":"https:\/\/doi.org\/10.1074\/jbc.ra119.011546","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2020,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"High-resolution crystal structures of two prototypical \u03b2- and \u03b3-herpesviral nuclear egress complexes unravel the determinants of subfamily specificity","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.RA119.011546","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Muller et al.","name":"copyright","label":"Copyright"}]}}