{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:34:25Z","timestamp":1791617665558,"version":"4.3.4"},"reference-count":45,"publisher":"Elsevier BV","issue":"51","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T00:00:00Z","timestamp":1610496000000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM127557"],"award-info":[{"award-number":["R01GM127557"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["RSG-19-01-CSM"],"award-info":[{"award-number":["RSG-19-01-CSM"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1074\/jbc.ra120.015398","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T15:45:12Z","timestamp":1603208712000},"page":"17672-17683","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":68,"title":["AggreCount: an unbiased image analysis tool for identifying and quantifying cellular aggregates in a spatially defined manner"],"prefix":"10.1016","volume":"295","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0759-4724","authenticated-orcid":false,"given":"Jacob Aaron","family":"Klickstein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8875-7985","authenticated-orcid":false,"given":"Sirisha","family":"Mukkavalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4773-8673","authenticated-orcid":false,"given":"Malavika","family":"Raman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/jbc.RA120.015398_bib1","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1038\/nature10317","article-title":"Molecular chaperones in protein folding and proteostasis","volume":"475","author":"Hartl","year":"2011","journal-title":"Nature"},{"key":"10.1074\/jbc.RA120.015398_bib2","doi-asserted-by":"crossref","first-page":"565","DOI":"10.3389\/fnins.2017.00565","article-title":"Molecular chaperones and neurodegeneration","volume":"11","author":"Roodveldt","year":"2017","journal-title":"Front. Neurosci"},{"key":"10.1074\/jbc.RA120.015398_bib3","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1005178","article-title":"The protein quality control machinery regulates its misassembled proteasome subunits","volume":"11","author":"Peters","year":"2015","journal-title":"PLoS Genet"},{"key":"10.1074\/jbc.RA120.015398_bib4","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3389\/fnins.2017.00064","article-title":"Cellular regulation of amyloid formation in aging and disease","volume":"11","author":"Stroo","year":"2017","journal-title":"Front. Neurosci"},{"key":"10.1074\/jbc.RA120.015398_bib5","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1126\/science.aag3048","article-title":"Lysosome activation clears aggregates and enhances quiescent neural stem cell activation during aging","volume":"359","author":"Leeman","year":"2018","journal-title":"Science"},{"key":"10.1074\/jbc.RA120.015398_bib6","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1038\/nm.4001","article-title":"Proteostasis and aging","volume":"21","author":"Kaushik","year":"2015","journal-title":"Nat. Med"},{"key":"10.1074\/jbc.RA120.015398_bib7","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.neuron.2016.02.003","article-title":"The biology of Huntingtin","volume":"89","author":"Saudou","year":"2016","journal-title":"Neuron"},{"key":"10.1074\/jbc.RA120.015398_bib8","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/674751","article-title":"ER dysfunction and protein folding stress in ALS","volume":"2013","author":"Matus","year":"2013","journal-title":"Int. J. Cell Biol"},{"key":"10.1074\/jbc.RA120.015398_bib9","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nrneurol.2012.242","article-title":"100 years of Lewy pathology","volume":"9","author":"Goedert","year":"2013","journal-title":"Nat. Rev. Neurol"},{"key":"10.1074\/jbc.RA120.015398_bib10","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1126\/science.1132814","article-title":"A century of Alzheimer's disease","volume":"314","author":"Goedert","year":"2006","journal-title":"Science"},{"key":"10.1074\/jbc.RA120.015398_bib11","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1146\/annurev-biochem-060815-014616","article-title":"Mechanisms and functions of spatial protein quality control","volume":"86","author":"Sontag","year":"2017","journal-title":"Annu. Rev. Biochem"},{"key":"10.1074\/jbc.RA120.015398_bib12","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1093\/hmg\/ddg074","article-title":"Aggresomes protect cells by enhancing the degradation of toxic polyglutamine-containing protein","volume":"12","author":"Taylor","year":"2003","journal-title":"Hum. Mol. Genet"},{"key":"10.1074\/jbc.RA120.015398_bib13","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.cell.2017.12.030","article-title":"In situ structure of neuronal C9orf72 poly-GA aggregates reveals proteasome recruitment","volume":"172","author":"Guo","year":"2018","journal-title":"Cell"},{"key":"10.1074\/jbc.RA120.015398_bib14","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.cell.2017.08.009","article-title":"In situ architecture and cellular interactions of PolyQ inclusions","volume":"171","author":"B\u00e4uerlein","year":"2017","journal-title":"Cell"},{"key":"10.1074\/jbc.RA120.015398_bib15","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.molcel.2013.08.016","article-title":"Proteasomes activate aggresome disassembly and clearance by producing unanchored ubiquitin chains","volume":"51","author":"Hao","year":"2013","journal-title":"Mol. Cell"},{"key":"10.1074\/jbc.RA120.015398_bib16","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4161\/auto.2731","article-title":"ALIS are stress-induced protein storage compartments for substrates of the proteasome and autophagy","volume":"2","author":"Szeto","year":"2006","journal-title":"Autophagy"},{"key":"10.1074\/jbc.RA120.015398_bib17","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.18413","article-title":"Distinct stages in stress granule assembly and disassembly","volume":"5","author":"Wheeler","year":"2016","journal-title":"eLife"},{"key":"10.1074\/jbc.RA120.015398_bib18","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1007\/s11095-010-0297-1","article-title":"Strategies for the assessment of protein aggregates in pharmaceutical biotech product development","volume":"28","author":"den Engelsman","year":"2011","journal-title":"Pharm. Res"},{"key":"10.1074\/jbc.RA120.015398_bib19","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms15730","article-title":"Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex","volume":"8","author":"Park","year":"2017","journal-title":"Nat. Commun"},{"key":"10.1074\/jbc.RA120.015398_bib20","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1242\/bio.20147021","article-title":"Aggresome formation is regulated by RanBPM through an interaction with HDAC6","volume":"3","author":"Salemi","year":"2014","journal-title":"Biol. Open"},{"key":"10.1074\/jbc.RA120.015398_bib21","doi-asserted-by":"crossref","first-page":"3773","DOI":"10.1158\/0008-5472.CAN-05-2961","article-title":"Aggresome disruption: a novel strategy to enhance bortezomib-induced apoptosis in pancreatic cancer cells","volume":"66","author":"Nawrocki","year":"2006","journal-title":"Cancer Res"},{"key":"10.1074\/jbc.RA120.015398_bib22","doi-asserted-by":"crossref","first-page":"8923","DOI":"10.1073\/pnas.0903621106","article-title":"Rheb controls misfolded protein metabolism by inhibiting aggresome formation and autophagy","volume":"106","author":"Zhou","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.RA120.015398_bib23","first-page":"4173","article-title":"14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes","volume":"126","author":"Xu","year":"2013","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.RA120.015398_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.2005970","article-title":"CellProfiler 3.0: next-generation image processing for biology","volume":"16","author":"McQuin","year":"2018","journal-title":"PLoS Biol"},{"key":"10.1074\/jbc.RA120.015398_bib25","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1074\/jbc.RA120.015398_bib26","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1038\/s41592-019-0403-1","article-title":"Deep learning for cellular image analysis","volume":"16","author":"Moen","year":"2019","journal-title":"Nat. Methods"},{"key":"10.1074\/jbc.RA120.015398_bib27","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.jneumeth.2013.12.003","article-title":"Evaluation of the effectiveness of Gaussian filtering in distinguishing punctate synaptic signals from background noise during image analysis","volume":"223","author":"Iwabuchi","year":"2014","journal-title":"J. Neurosci. Methods"},{"key":"10.1074\/jbc.RA120.015398_bib28","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/S0962-8924(00)01852-3","article-title":"Aggresomes, inclusion bodies and protein aggregation","volume":"10","author":"Kopito","year":"2000","journal-title":"Trends Cell Biol"},{"key":"10.1074\/jbc.RA120.015398_bib29","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1016\/j.csbj.2020.04.014","article-title":"The science of puromycin: from studies of ribosome function to applications in biotechnology","volume":"18","author":"Aviner","year":"2020","journal-title":"Comput. Struct. Biotechnol. J"},{"key":"10.1074\/jbc.RA120.015398_bib30","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/s41580-019-0118-2","article-title":"Mechanisms and functions of ribosome-associated protein quality control","volume":"20","author":"Joazeiro","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol"},{"key":"10.1074\/jbc.RA120.015398_bib31","doi-asserted-by":"crossref","first-page":"330","DOI":"10.4161\/auto.6.3.11226","article-title":"p62\/SQSTM1 and ALFY interact to facilitate the formation of p62 bodies\/ALIS and their degradation by autophagy","volume":"6","author":"Clausen","year":"2010","journal-title":"Autophagy"},{"key":"10.1074\/jbc.RA120.015398_bib32","doi-asserted-by":"crossref","first-page":"e00154","DOI":"10.1128\/MCB.00154-18","article-title":"The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex","volume":"38","author":"Ganji","year":"2018","journal-title":"Mol. Cell Biol"},{"key":"10.1074\/jbc.RA120.015398_bib33","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.tcb.2016.05.004","article-title":"Principles and properties of stress granules","volume":"26","author":"Protter","year":"2016","journal-title":"Trends Cell Biol"},{"key":"10.1074\/jbc.RA120.015398_bib34","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/s41583-019-0222-5","article-title":"Stress granules and neurodegeneration","volume":"20","author":"Wolozin","year":"2019","journal-title":"Nat. Rev. Neurosci"},{"key":"10.1074\/jbc.RA120.015398_bib35","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.molcel.2019.03.027","article-title":"ULK1 and ULK2 regulate stress granule disassembly through phosphorylation and activation of VCP\/p97","volume":"74","author":"Wang","year":"2019","journal-title":"Mol. Cell"},{"key":"10.1074\/jbc.RA120.015398_bib36","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/j.molcel.2018.04.021","article-title":"ZFAND1 recruits p97 and the 26S proteasome to promote the clearance of arsenite-induced stress granules","volume":"70","author":"Turakhiya","year":"2018","journal-title":"Mol. Cell"},{"key":"10.1074\/jbc.RA120.015398_bib37","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.cell.2015.12.038","article-title":"ATPase-modulated stress granules contain a diverse proteome and substructure","volume":"164","author":"Jain","year":"2016","journal-title":"Cell"},{"key":"10.1074\/jbc.RA120.015398_bib38","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.ccell.2015.10.002","article-title":"Targeting the AAA ATPase p97 as an approach to treat cancer through disruption of protein homeostasis","volume":"28","author":"Anderson","year":"2015","journal-title":"Cancer Cell"},{"key":"10.1074\/jbc.RA120.015398_bib39","doi-asserted-by":"crossref","first-page":"4604","DOI":"10.1073\/pnas.96.8.4604","article-title":"Self-assembly of polyglutamine-containing huntingtin fragments into amyloid-like fibrils: implications for Huntington's disease pathology","volume":"96","author":"Scherzinger","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/jbc.RA120.015398_bib40","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1083\/jcb.201511024","article-title":"Protein misfolding specifies recruitment to cytoplasmic inclusion bodies","volume":"213","author":"Bersuker","year":"2016","journal-title":"J. Cell Biol"},{"key":"10.1074\/jbc.RA120.015398_bib41","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1242\/jcs.115.5.941","article-title":"Perinuclear localization of huntingtin as a consequence of its binding to microtubules through an interaction with \u03b2-tubulin: relevance to Huntington's disease","volume":"115","author":"Hoffner","year":"2002","journal-title":"J. Cell Sci"},{"key":"10.1074\/jbc.RA120.015398_bib42","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1038\/nature02998","article-title":"Inclusion body formation reduces levels of mutant huntingtin and the risk of neuronal death","volume":"431","author":"Arrasate","year":"2004","journal-title":"Nature"},{"key":"10.1074\/jbc.RA120.015398_bib43","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/jmi.12184","article-title":"Review of free software tools for image analysis of fluorescence cell micrographs","volume":"257","author":"Wiesmann","year":"2015","journal-title":"J. Microsc"},{"key":"10.1074\/jbc.RA120.015398_bib44","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1074\/jbc.RA120.015398_bib45","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1038\/ncb3238","article-title":"Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis","volume":"17","author":"Raman","year":"2015","journal-title":"Nat. Cell Biol"}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925817506481?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925817506481?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/jbc.RA120.015398","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T12:22:29Z","timestamp":1640953349000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925817506481"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":45,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["S0021925817506481"],"URL":"https:\/\/doi.org\/10.1074\/jbc.ra120.015398","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.07.25.221267","asserted-by":"object"}]},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"AggreCount: an unbiased image analysis tool for identifying and quantifying cellular aggregates in a spatially defined manner","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/jbc.RA120.015398","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Klickstein et al.","name":"copyright","label":"Copyright"}]}}