{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T09:03:08Z","timestamp":1778058188104,"version":"3.51.4"},"reference-count":38,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,10,11]],"date-time":"2020-10-11T00:00:00Z","timestamp":1602374400000},"content-version":"vor","delay-in-days":2110,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["mcponline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular & Cellular Proteomics"],"published-print":{"date-parts":[[2015,1]]},"DOI":"10.1074\/mcp.m114.043513","type":"journal-article","created":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T22:04:55Z","timestamp":1415052295000},"page":"136-147","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":26,"title":["Epitope Identification from Fixed-complexity Random-sequence Peptide Microarrays"],"prefix":"10.1016","volume":"14","author":[{"given":"Josh","family":"Richer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen Albert","family":"Johnston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip","family":"Stafford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/mcp.M114.043513_bib1","doi-asserted-by":"crossref","first-page":"E3072","DOI":"10.1073\/pnas.1409432111","article-title":"Immunosignature system for diagnosis of cancer","volume":"111","author":"Stafford","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"issue":"48","key":"10.1074\/mcp.M114.043513_bib2","doi-asserted-by":"crossref","first-page":"19330","DOI":"10.1073\/pnas.1314792110","article-title":"Antibody biomarker discovery through in vitro directed evolution of consensus recognition epitopes","volume":"110","author":"Ballew","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1074\/mcp.M114.043513_bib3","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0022-1759(97)00083-5","article-title":"Epitope mapping by phage display: random versus gene-fragment libraries","volume":"206","author":"Fack","year":"1997","journal-title":"J. Immunol. Methods"},{"key":"10.1074\/mcp.M114.043513_bib4","series-title":"Antibody Engineering","first-page":"443","article-title":"Antibody epitope mapping using arrays of synthetic peptides","author":"Reineke","year":"2004"},{"key":"10.1074\/mcp.M114.043513_bib5","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1056\/NEJMoa1211721","article-title":"Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia","volume":"367","author":"Zaki","year":"2012","journal-title":"N. Engl. J. Med"},{"key":"10.1074\/mcp.M114.043513_bib6","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s00253-005-0270-9","article-title":"Phage display systems and their applications","volume":"70","author":"Paschke","year":"2006","journal-title":"Appl. Microbiol. Biotechnol"},{"key":"10.1074\/mcp.M114.043513_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2174\/1389450043490668","article-title":"Epitope identification and discovery using phage display libraries: applications in vaccine development and diagnostics","volume":"5","author":"Wang","year":"2004","journal-title":"Curr. Drug Targets"},{"key":"10.1074\/mcp.M114.043513_bib8","doi-asserted-by":"crossref","first-page":"125","DOI":"10.2174\/1386207302666220203195547","article-title":"Epitope discovery using monoclonal antibodies and phage peptide libraries","volume":"2","author":"Yip","year":"1999","journal-title":"Comb. Chem. High Throughput Screen"},{"key":"10.1074\/mcp.M114.043513_bib9","doi-asserted-by":"crossref","first-page":"4210","DOI":"10.1002\/pmic.200500606","article-title":"T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries","volume":"6","author":"Krumpe","year":"2006","journal-title":"Proteomics"},{"key":"10.1074\/mcp.M114.043513_bib10","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1093\/bioinformatics\/btr060","article-title":"Phage display can select over-hydrophobic sequences that may impair prediction of natural domain\u2013peptide interactions","volume":"27","author":"Luck","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1074\/mcp.M114.043513_bib11","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0022-2836(02)00844-6","article-title":"Quantitative assessment of peptide sequence diversity in M13 combinatorial peptide phage display libraries","volume":"322","author":"Rodi","year":"2002","journal-title":"J. Mol. Biol"},{"key":"10.1074\/mcp.M114.043513_bib12","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1074\/mcp.M112.020800","article-title":"High-resolution mapping of linear antibody epitopes using ultra high-density peptide microarrays","volume":"11","author":"Buus","year":"2012","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.M114.043513_bib13","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.molimm.2012.03.030","article-title":"An analysis of B-cell epitope discontinuity","volume":"51","author":"Sivalingam","year":"2012","journal-title":"Mol. Immunol"},{"key":"10.1074\/mcp.M114.043513_bib14","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1074\/mcp.M113.033308","article-title":"Proteome-wide epitope mapping of antibodies using ultra-dense peptide arrays","volume":"13","author":"Forsstr\u00f6m","year":"2014","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.M114.043513_bib15","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.1074\/mcp.M113.034140","article-title":"Immunoproteomics using polyclonal antibodies and stable isotope\u2013labeled affinity-purified recombinant proteins","volume":"13","author":"Edfors","year":"2014","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.M114.043513_bib16","doi-asserted-by":"crossref","first-page":"e68902","DOI":"10.1371\/journal.pone.0068902","article-title":"Identification and mapping of linear antibody epitopes in human serum albumin using high-density peptide arrays","volume":"8","author":"Hansen","year":"2013","journal-title":"PLoS One"},{"key":"10.1074\/mcp.M114.043513_bib17","series-title":"Epitope Mapping Protocols","first-page":"145","article-title":"Antibody epitope mapping using SPOT\u2122 peptide arrays","author":"Reineke","year":"2009"},{"key":"10.1074\/mcp.M114.043513_bib18","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.tibtech.2012.10.012","article-title":"Immunosignaturing: a critical review","volume":"31","author":"Sykes","year":"2013","journal-title":"Trends Biotechnol"},{"issue":"4","key":"10.1074\/mcp.M114.043513_bib19","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M111.011593","article-title":"Physical characterization of the \u201cimmunosignaturing effect.\u201d","volume":"11","author":"Stafford","year":"2012","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.M114.043513_bib20","doi-asserted-by":"crossref","first-page":"e40201","DOI":"10.1371\/journal.pone.0040201","article-title":"Immunosignaturing can detect products from molecular markers in brain cancer","volume":"7","author":"Hughes","year":"2012","journal-title":"PLoS One"},{"key":"10.1074\/mcp.M114.043513_bib21","doi-asserted-by":"crossref","first-page":"4529","DOI":"10.1016\/j.vaccine.2010.04.061","article-title":"A general method for characterization of humoral immunity induced by a vaccine or infection","volume":"28","author":"Legutki","year":"2010","journal-title":"Vaccine"},{"issue":"1\u20132","key":"10.1074\/mcp.M114.043513_bib22","first-page":"154","article-title":"Feasibility of an early Alzheimer's disease immunosignature diagnostic test","volume":"254","author":"Restrepo","year":"2012","journal-title":"J. Neuroimmunol"},{"issue":"2","key":"10.1074\/mcp.M114.043513_bib23","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/ana.22405","article-title":"Application of immunosignatures to the assessment of Alzheimer's disease","volume":"5\u201318 70","author":"Restrepo","year":"2011","journal-title":"Ann. Neurol"},{"issue":"3","key":"10.1074\/mcp.M114.043513_bib24","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M110.000786","article-title":"Exploring antibody recognition of sequence space through random-sequence peptide microarrays","volume":"10, 10","author":"Halperin","year":"2011","journal-title":"Mol. Cell. Proteomics"},{"issue":"5","key":"10.1074\/mcp.M114.043513_bib25","doi-asserted-by":"crossref","first-page":"4785","DOI":"10.1038\/ncomms5785","article-title":"Scalable high-density peptide arrays for comprehensive health monitoring","volume":"5","author":"Legutki","year":"2014","journal-title":"Nat. Commun"},{"key":"10.1074\/mcp.M114.043513_bib26","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1002\/j.1460-2075.1995.tb07272.x","article-title":"A new type of signal peptide: central role of a twin-arginine motif in transfer signals for the delta pH-dependent thylakoidal protein translocase","volume":"14","author":"Chaddock","year":"1995","journal-title":"EMBO J"},{"key":"10.1074\/mcp.M114.043513_bib27","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0014-5793(01)03239-2","article-title":"Calmodulin signaling via the IQ motif","volume":"513","author":"B\u00e4hler","year":"2002","journal-title":"FEBS Lett"},{"key":"10.1074\/mcp.M114.043513_bib28","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nrd2241","article-title":"Development trends for monoclonal antibody cancer therapeutics","volume":"6","author":"Reichert","year":"2007","journal-title":"Nat. Rev. Drug Discov"},{"key":"10.1074\/mcp.M114.043513_bib29","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1128\/CVI.00228-14","article-title":"Application of immunosignatures for diagnosis of valley fever","volume":"21","author":"Navalkar","year":"2014","journal-title":"Clin. Vaccine Immunol"},{"key":"10.1074\/mcp.M114.043513_bib30","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/1471-2172-15-23","article-title":"Diagnosis and early detection of CNS-SLE in MRL\/lpr mice using peptide microarrays","volume":"15","author":"Williams","year":"2014","journal-title":"BMC Immunol"},{"key":"10.1074\/mcp.M114.043513_bib31","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1128\/CVI.05653-11","article-title":"Antibodies to Borrelia burgdorferi OspA, OspC, OspF, and C6 antigens as markers for early and late infection in dogs","volume":"19","author":"Wagner","year":"2012","journal-title":"Clin. Vaccine Immunol"},{"key":"10.1074\/mcp.M114.043513_bib32","article-title":"Immunosignaturing microarrays distinguish antibody profiles of related pancreatic diseases","volume":"S6","author":"Kukreja","year":"2012","journal-title":"Proteomics Bioinformatics"},{"key":"10.1074\/mcp.M114.043513_bib33","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.virol.2009.12.010","article-title":"Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1","volume":"398","author":"Chen","year":"2010","journal-title":"Virology"},{"key":"10.1074\/mcp.M114.043513_bib34","doi-asserted-by":"crossref","first-page":"466","DOI":"10.4269\/ajtmh.1997.56.466","article-title":"Recognition of synthetic oligopeptides from nonstructural proteins NS1 and NS3 of dengue-4 virus by sera from dengue virus-infected children","volume":"56","author":"Garcia","year":"1997","journal-title":"Am. J. Trop. Med. Hyg"},{"issue":"2","key":"10.1074\/mcp.M114.043513_bib35","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/srep00267","article-title":"Hepatitis C virus antigenic convergence","volume":"2","author":"Campo","year":"2012","journal-title":"Sci. Rep"},{"key":"10.1074\/mcp.M114.043513_bib36","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1016\/j.biocel.2008.12.012","article-title":"PfEMP1: an antigen that plays a key role in the pathogenicity and immune evasion of the malaria parasite Plasmodium falciparum","volume":"41","author":"Pasternak","year":"2009","journal-title":"Int. J. Biochem. Cell Biol"},{"key":"10.1074\/mcp.M114.043513_bib37","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1365-3024.1986.tb00867.x","article-title":"Multiple cross-reactivities amongst antigens of Plasmodium falciparum impair the development of protective immunity against malaria","volume":"8","author":"Anders","year":"1986","journal-title":"Parasite Immunol"},{"key":"10.1074\/mcp.M114.043513_bib38","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature01097","article-title":"Genome sequence of the human malaria parasite Plasmodium falciparum","volume":"419","author":"Gardner","year":"2002","journal-title":"Nature"}],"container-title":["Molecular & Cellular Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620316686?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620316686?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/mcp.M114.043513","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:30:17Z","timestamp":1761525017000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1535947620316686"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["S1535947620316686"],"URL":"https:\/\/doi.org\/10.1074\/mcp.m114.043513","relation":{},"ISSN":["1535-9476"],"issn-type":[{"value":"1535-9476","type":"print"}],"subject":[],"published":{"date-parts":[[2015,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Epitope Identification from Fixed-complexity Random-sequence Peptide Microarrays","name":"articletitle","label":"Article Title"},{"value":"Molecular & Cellular Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/mcp.M114.043513","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2015 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}