{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:30:34Z","timestamp":1791567034016,"version":"4.3.4"},"reference-count":47,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"vor","delay-in-days":4097,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["mcponline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular & Cellular Proteomics"],"published-print":{"date-parts":[[2009,7]]},"DOI":"10.1074\/mcp.m800556-mcp200","type":"journal-article","created":{"date-parts":[[2009,4,5]],"date-time":"2009-04-05T20:12:37Z","timestamp":1238962357000},"page":"1527-1538","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":164,"title":["The Phosphoproteome of Bloodstream Form Trypanosoma brucei, Causative Agent of African Sleeping Sickness"],"prefix":"10.1016","volume":"8","author":[{"given":"Isabelle R.E.","family":"Nett","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.A.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Miranda-Saavedra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Lamont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan D.","family":"Barber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Mehlert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.J.","family":"Ferguson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/mcp.M800556-MCP200_bib1","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/S0968-0004(00)01712-6","article-title":"The regulation of protein function by multisite phosphorylation: a 25 year update","volume":"25","author":"Cohen","year":"2000","journal-title":"Trends Biochem. Sci."},{"key":"10.1074\/mcp.M800556-MCP200_bib2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1096\/fasebj.9.8.7768349","article-title":"Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification","volume":"9","author":"Hanks","year":"1995","journal-title":"FASEB J."},{"key":"10.1074\/mcp.M800556-MCP200_bib3","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.bbapap.2005.08.018","article-title":"Protein kinases as drug targets in trypanosomes and Leishmania","volume":"1754","author":"Naula","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1074\/mcp.M800556-MCP200_bib4","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1172\/JCI33945","article-title":"Kinetoplastids: related protozoan pathogens, different diseases","volume":"118","author":"Stuart","year":"2008","journal-title":"J. Clin. Investig."},{"key":"10.1074\/mcp.M800556-MCP200_bib5","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1186\/1471-2164-6-127","article-title":"Comparative analysis of the kinomes of three pathogenic trypanosomatids: Leishmania major, Trypanosoma brucei Trypanosoma cruzi.","volume":"6","author":"Parsons","year":"2005","journal-title":"BMC Genomics"},{"key":"10.1074\/mcp.M800556-MCP200_bib6","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1002\/prot.21444","article-title":"Classification and functional annotation of eukaryotic protein kinases","volume":"68","author":"Miranda-Saavedra","year":"2007","journal-title":"Proteins"},{"key":"10.1074\/mcp.M800556-MCP200_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molbiopara.2007.01.017","article-title":"Cell cycle regulation in Trypanosoma brucei","volume":"153","author":"Hammarton","year":"2007","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/mcp.M800556-MCP200_bib8","doi-asserted-by":"crossref","first-page":"20519","DOI":"10.1074\/jbc.M312862200","article-title":"The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion","volume":"279","author":"Tu","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.M800556-MCP200_bib9","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1128\/EC.4.4.755-764.2005","article-title":"Pairwise knockdowns of cdc2-related kinases (CRKs) in Trypanosoma brucei identified the CRKs for G1\/S and G2\/M transitions and demonstrated distinctive cytokinetic regulations between two developmental stages of the organism","volume":"4","author":"Tu","year":"2005","journal-title":"Eukaryot. Cell"},{"key":"10.1074\/mcp.M800556-MCP200_bib10","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1111\/j.1365-2958.2004.04218.x","article-title":"A novel ERK-like, CRK-like protein kinase that modulates growth in Trypanosoma brucei via an autoregulatory Cterminal extension","volume":"53","author":"Ellis","year":"2004","journal-title":"Mol. Microbiol."},{"key":"10.1074\/mcp.M800556-MCP200_bib11","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1111\/j.1365-2958.2007.05866.x","article-title":"Trypanosoma brucei Polo-like kinase is essential for basal body duplication, kDNA segregation and cytokinesis","volume":"65","author":"Hammarton","year":"2007","journal-title":"Mol. Microbiol."},{"key":"10.1074\/mcp.M800556-MCP200_bib12","doi-asserted-by":"crossref","first-page":"27600","DOI":"10.1074\/jbc.M602183200","article-title":"TbVps34, the trypanosome orthologue of Vps34, is required for Golgi complex segregation","volume":"281","author":"Hall","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.M800556-MCP200_bib13","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.tibs.2005.04.013","article-title":"Protein phosphorylation in signaling: 50 years and counting","volume":"30","author":"Pawson","year":"2005","journal-title":"Trends Biochem. Sci."},{"key":"10.1074\/mcp.M800556-MCP200_bib14","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0166-6851(98)00168-6","article-title":"Trypanosoma brucei GPEETPARP is phosphorylated on six out of seven threonine residues","volume":"98","author":"Mehlert","year":"1999","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/mcp.M800556-MCP200_bib15","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.molbiopara.2004.12.007","article-title":"Trypanosoma cruzi histone H1 is phosphorylated in a typical cyclin dependent kinase site accordingly to the cell cycle","volume":"140","author":"da Cunha","year":"2005","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/mcp.M800556-MCP200_bib16","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/nbt0302-301","article-title":"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae","volume":"20","author":"Ficarro","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"10.1074\/mcp.M800556-MCP200_bib17","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1074\/mcp.T300006-MCP200","article-title":"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry","volume":"2","author":"N\u00fcumlhse","year":"2003","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.M800556-MCP200_bib18","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1021\/pr0503073","article-title":"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS\/MS","volume":"5","author":"Moser","year":"2006","journal-title":"J. Proteome Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib19","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1074\/mcp.M600464-MCP200","article-title":"The serine\/threonine\/tyrosine phosphoproteome of the model bacterium Bacillus subtilis","volume":"6","author":"Macek","year":"2007","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.M800556-MCP200_bib20","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1074\/mcp.M700311-MCP200","article-title":"Phosphoproteome analysis of E.coli reveals evolutionary conservation of bacterial Ser\/Thr\/Tyr phosphorylation","volume":"7","author":"Macek","year":"2008","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.M800556-MCP200_bib21","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0166-6851(01)00257-2","article-title":"Virulence of Trypanosoma brucei strain 427 is not affected by the absence of glycosylphosphatidyl phospholipase C","volume":"114","author":"Leal","year":"2001","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1074\/mcp.M800556-MCP200_bib22","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/jcb.240240107","volume":"24","author":"Cross","year":"1984","journal-title":"J. Cell. Biochem."},{"key":"10.1074\/mcp.M800556-MCP200_bib23","doi-asserted-by":"crossref","first-page":"12130","DOI":"10.1073\/pnas.0404720101","article-title":"Large-scale characterization of HeLa cell nuclear phosphoproteins","volume":"101","author":"Beausoleil","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1074\/mcp.M800556-MCP200_bib24","doi-asserted-by":"crossref","first-page":"D539","DOI":"10.1093\/nar\/gkn814","article-title":"PlasmoDB: a functional genomic database for malaria parasites","volume":"37","author":"Aurrecoechea","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib25","doi-asserted-by":"crossref","first-page":"3625","DOI":"10.1093\/nar\/gkg545","article-title":"ELM server: a new resource for investigating short functional sites in modular eukaryotic proteins","volume":"31","author":"Puntervoll","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib26","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/S0168-9525(00)02024-2","article-title":"EMBOSS: the European Molecular Biology Open Software Suite","volume":"16","author":"Rice","year":"2000","journal-title":"Trends Genet."},{"key":"10.1074\/mcp.M800556-MCP200_bib27","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/1471-2105-5-79","article-title":"Phospho.ELM: a database of experimentally verified phosphorylation sites in eukaryotic proteins","volume":"5","author":"Diella","year":"2004","journal-title":"BMC Bioinformatics"},{"key":"10.1074\/mcp.M800556-MCP200_bib28","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1186\/1471-2164-8-309","article-title":"The complement of protein kinases of the microsporidium Encephalitozoon cuniculi in relation to those of Saccharomyces cerevisiae Schizosaccharomyces pombe","volume":"8","author":"Miranda-Saavedra","year":"2007","journal-title":"BMC Genomics"},{"key":"10.1074\/mcp.M800556-MCP200_bib29","doi-asserted-by":"crossref","first-page":"22948","DOI":"10.1074\/jbc.M100087200","article-title":"The WD motif-containing protein receptor for activated protein kinase C (RACK1) is required for recruitment and activation of signal transducer and activator of transcription 1 through the type I interferon receptor","volume":"276","author":"Usacheva","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.M800556-MCP200_bib30","doi-asserted-by":"crossref","first-page":"9781","DOI":"10.1074\/jbc.M600133200","article-title":"The RACK1 homologue from Trypanosoma brucei is required for the onset and progression of cytokinesis","volume":"281","author":"Rothberg","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.M800556-MCP200_bib31","doi-asserted-by":"crossref","first-page":"3635","DOI":"10.1093\/nar\/gkg584","article-title":"Scansite 2.0: proteome-wide prediction of cell signaling interactions using short sequence motifs","volume":"31","author":"Obenauer","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib32","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.cell.2006.09.026","article-title":"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks","volume":"127","author":"Olsen","year":"2006","journal-title":"Cell"},{"key":"10.1074\/mcp.M800556-MCP200_bib33","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/1471-2164-5-79","article-title":"Protein kinases of the human malaria parasite Plasmodium falciparum: the kinome of a divergent eukaryote","volume":"5","author":"Ward","year":"2004","journal-title":"BMC Genomics"},{"key":"10.1074\/mcp.M800556-MCP200_bib34","doi-asserted-by":"crossref","first-page":"D553","DOI":"10.1093\/nar\/gkm981","article-title":"ToxoDB: an integrated Toxoplasma gondii database resource","volume":"36","author":"Gajria","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib35","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1128\/EC.5.1.92-102.2006","article-title":"Dissociation of cytokinesis initiation from mitotic control in a eukaryote","volume":"5","author":"Kumar","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"10.1074\/mcp.M800556-MCP200_bib36","doi-asserted-by":"crossref","first-page":"14085","DOI":"10.1074\/jbc.M412336200","article-title":"The 14-3-3 proteins of Trypanosoma brucei function in motility, cytokinesis, and cell cycle","volume":"280","author":"Inoue","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.M800556-MCP200_bib37","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/j.jmb.2004.04.043","article-title":"Structural modes of stabilization of permissive phosphorylation sites in protein kinases: distinct strategies in Ser\/Thr and Tyr kinases","volume":"339","author":"Krupa","year":"2004","journal-title":"J. Mol. Biol."},{"key":"10.1074\/mcp.M800556-MCP200_bib38","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1074\/jbc.271.7.3488","article-title":"Dyrk, a dual specificity protein kinase with unique structural features whose activity is dependent on tyrosine residues between subdomains VII and VIII","volume":"271","author":"Kentrup","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1074\/mcp.M800556-MCP200_bib39","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1042\/bj20030500","article-title":"dDYRK2: a novel dual-specificity tyrosine phosphorylation regulated kinase in Drosophila","volume":"374","author":"Lochhead","year":"2003","journal-title":"Biochem. J."},{"key":"10.1074\/mcp.M800556-MCP200_bib40","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1042\/bj20031259","article-title":"Further evidence that the tyrosine phosphorylation of glycogen synthase kinase-3 (GSK3) in mammalian cells is an autophosphorylation event","volume":"377","author":"Cole","year":"2004","journal-title":"Biochem. J."},{"key":"10.1074\/mcp.M800556-MCP200_bib41","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1042\/bj3030701","article-title":"Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation","volume":"303","author":"Stambolic","year":"1994","journal-title":"Biochem. J."},{"key":"10.1074\/mcp.M800556-MCP200_bib42","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1046\/j.1365-2443.1999.00261.x","article-title":"Molecular cloning and characterization of a novel member of the MAP kinase superfamily","volume":"4","author":"Miyata","year":"1999","journal-title":"Genes Cells"},{"key":"10.1074\/mcp.M800556-MCP200_bib43","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1128\/EC.00094-06","article-title":"A mitogen-activated protein kinase controls differentiation of bloodstream forms of Trypanosoma brucei","volume":"5","author":"Domenicali Pfister","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"10.1074\/mcp.M800556-MCP200_bib44","doi-asserted-by":"crossref","first-page":"D339","DOI":"10.1093\/nar\/gkh007","article-title":"GeneDB: a resource for prokaryotic and eukaryotic organisms","volume":"32","author":"Hertz-Fowler","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib45","doi-asserted-by":"crossref","first-page":"D707","DOI":"10.1093\/nar\/gkm988","article-title":"Ensembl 2008","volume":"36","author":"Flicek","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib46","doi-asserted-by":"crossref","first-page":"D442","DOI":"10.1093\/nar\/gkj117","article-title":"Genome Snapshot: a new resource at the Saccharomyces Genome Database (SGD) presenting an overview of the Saccharomyces cerevisiae genome","volume":"34","author":"Hirschman","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1074\/mcp.M800556-MCP200_bib47","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1038\/35106579","article-title":"Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi.","volume":"414","author":"Katinka","year":"2001","journal-title":"Nature"}],"container-title":["Molecular & Cellular Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620340330?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620340330?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/mcp.M800556-MCP200","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:57:37Z","timestamp":1761526657000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1535947620340330"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["S1535947620340330"],"URL":"https:\/\/doi.org\/10.1074\/mcp.m800556-mcp200","relation":{},"ISSN":["1535-9476"],"issn-type":[{"value":"1535-9476","type":"print"}],"subject":[],"published":{"date-parts":[[2009,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The Phosphoproteome of Bloodstream Form Trypanosoma brucei, Causative Agent of African Sleeping Sickness","name":"articletitle","label":"Article Title"},{"value":"Molecular & Cellular Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/mcp.M800556-MCP200","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2009 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}