{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:58:45Z","timestamp":1791046725850,"version":"4.1.0"},"reference-count":52,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T00:00:00Z","timestamp":1601337600000},"content-version":"vor","delay-in-days":2524,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["mcponline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular &amp; Cellular Proteomics"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1074\/mcp.o113.027342","type":"journal-article","created":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T13:43:03Z","timestamp":1374673383000},"page":"3339-3349","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["Development of a 5-plex SILAC Method Tuned for the Quantitation of Tyrosine Phosphorylation Dynamics"],"prefix":"10.1016","volume":"12","author":[{"given":"Manuel","family":"Tzouros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Golling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Avila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Lamerz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Berrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Ebeling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanno","family":"Langen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lique","family":"Augustin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1074\/mcp.O113.027342_bib1","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/S0968-0004(00)01712-6","article-title":"The regulation of protein function by multisite phosphorylation\u2014a 25 year update","volume":"25","author":"Cohen","year":"2000","journal-title":"Trends Biochem. Sci"},{"key":"10.1074\/mcp.O113.027342_bib2","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1042\/BST0370627","article-title":"The regulation of protein phosphorylation","volume":"37","author":"Johnson","year":"2009","journal-title":"Biochem. Soc. Trans"},{"key":"10.1074\/mcp.O113.027342_bib3","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/j.cell.2010.06.011","article-title":"Cell signaling by receptor tyrosine kinases","volume":"141","author":"Lemmon","year":"2010","journal-title":"Cell"},{"key":"10.1074\/mcp.O113.027342_bib4","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.ceb.2009.01.028","article-title":"Tyrosine phosphorylation: thirty years and counting","volume":"21","author":"Hunter","year":"2009","journal-title":"Curr. Opin. Cell Biol"},{"key":"10.1074\/mcp.O113.027342_bib5","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/nrm1962","article-title":"EGF-ERBB signalling: towards the systems level","volume":"7","author":"Citri","year":"2006","journal-title":"Nat. Rev. Mol. Cell. Biol"},{"key":"10.1074\/mcp.O113.027342_bib6","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1038\/35077225","article-title":"Oncogenic kinase signalling","volume":"411","author":"Blume-Jensen","year":"2001","journal-title":"Nature"},{"key":"10.1074\/mcp.O113.027342_bib7","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.ceb.2008.12.010","article-title":"ErbB receptors and signaling pathways in cancer","volume":"21","author":"Hynes","year":"2009","journal-title":"Curr. Opin. Cell Biol"},{"key":"10.1074\/mcp.O113.027342_bib8","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ceb.2007.02.008","article-title":"The EGF receptor family: spearheading a merger of signaling and therapeutics","volume":"19","author":"Bublil","year":"2007","journal-title":"Curr. Opin. Cell Biol"},{"key":"10.1074\/mcp.O113.027342_bib9","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1021\/ac202877y","article-title":"Advances in quantitative phosphoproteomics","volume":"84","author":"Nilsson","year":"2012","journal-title":"Anal. Chem"},{"key":"10.1074\/mcp.O113.027342_bib10","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/nrm2900","article-title":"Decoding signalling networks by mass spectrometry-based proteomics","volume":"11","author":"Choudhary","year":"2010","journal-title":"Nat. Rev. Mol. Cell. Biol"},{"key":"10.1074\/mcp.O113.027342_bib11","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1586\/epr.10.19","article-title":"Strategies for quantitation of phosphoproteomic data","volume":"7","author":"Palmisano","year":"2010","journal-title":"Expert Rev. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib12","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1002\/pmic.200800231","article-title":"Quantitative phosphoproteomics by mass spectrometry: past, present, and future","volume":"8","author":"Nita-Lazar","year":"2008","journal-title":"Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib13","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1016\/j.copbio.2008.06.006","article-title":"Quantitative phosphoproteomic analysis of signaling network dynamics","volume":"19","author":"White","year":"2008","journal-title":"Curr. Opin. Biotechnol"},{"key":"10.1074\/mcp.O113.027342_bib14","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.gde.2009.12.005","article-title":"Quantifying oncogenic phosphotyrosine signaling networks through systems biology","volume":"20","author":"Del Rosario","year":"2010","journal-title":"Curr. Opin. Genet. Dev"},{"key":"10.1074\/mcp.O113.027342_bib15","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1039\/b909534a","article-title":"Receptor tyrosine kinase signaling: a view from quantitative proteomics","volume":"5","author":"Dengjel","year":"2009","journal-title":"Mol. Biosyst"},{"key":"10.1074\/mcp.O113.027342_bib16","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1146\/annurev.pharmtox.011008.145606","article-title":"Global and site-specific quantitative phosphoproteomics: principles and applications","volume":"49","author":"Macek","year":"2009","journal-title":"Annu. Rev. Pharmacol. Toxicol"},{"key":"10.1074\/mcp.O113.027342_bib17","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.cell.2006.09.026","article-title":"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks","volume":"127","author":"Olsen","year":"2006","journal-title":"Cell"},{"key":"10.1074\/mcp.O113.027342_bib18","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1074\/mcp.M500089-MCP200","article-title":"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules","volume":"4","author":"Zhang","year":"2005","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib19","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M112.019984","article-title":"Molecular characterization of EGFR and EGFRvIII signaling networks in human glioblastoma tumor xenografts","volume":"11","author":"Johnson","year":"2012","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib20","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1074\/mcp.M800029-MCP200","article-title":"Robust and sensitive iTRAQ quantification on an LTQ Orbitrap mass spectrometer","volume":"7","author":"Bantscheff","year":"2008","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib21","doi-asserted-by":"crossref","first-page":"5347","DOI":"10.1021\/pr900634c","article-title":"iTRAQ underestimation in simple and complex mixtures: \u201cthe good, the bad and the ugly.\u201d","volume":"8","author":"Ow","year":"2009","journal-title":"J. Proteome Res"},{"key":"10.1074\/mcp.O113.027342_bib22","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1074\/mcp.M900628-MCP200","article-title":"Addressing accuracy and precision issues in iTRAQ quantitation","volume":"9","author":"Karp","year":"2010","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib23","doi-asserted-by":"crossref","first-page":"4045","DOI":"10.1021\/pr100230q","article-title":"Undesirable charge-enhancement of isobaric tagged phosphopeptides leads to reduced identification efficiency","volume":"9","author":"Thingholm","year":"2010","journal-title":"J. Proteome Res"},{"key":"10.1074\/mcp.O113.027342_bib24","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1074\/mcp.M800113-MCP200","article-title":"Prevention of amino acid conversion in SILAC experiments with embryonic stem cells","volume":"7","author":"Bendall","year":"2008","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib25","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/sj.bjc.6690114","article-title":"Isolation and characterization of a new human breast cancer cell line, KPL-4, expressing the Erb B family receptors and interleukin-6","volume":"79","author":"Kurebayashi","year":"1999","journal-title":"Br. J. Cancer"},{"key":"10.1074\/mcp.O113.027342_bib26","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1074\/mcp.M900291-MCP200","article-title":"In depth qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immuno-affinity purification and stable isotope dimethyl labeling","volume":"9","author":"Boersema","year":"2009","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib27","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1074\/mcp.T500030-MCP200","article-title":"Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap","volume":"4","author":"Olsen","year":"2005","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib28","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M110.003830","article-title":"Confident phosphorylation site localization using the mascot delta score","volume":"10","author":"Savitski","year":"2011","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib29","doi-asserted-by":"crossref","first-page":"15805","DOI":"10.1074\/jbc.M311714200","article-title":"A novel proteomic approach for specific identification of tyrosine kinase substrates using [13C]tyrosine","volume":"279","author":"Ibarrola","year":"2004","journal-title":"J. Biol. Chem"},{"key":"10.1074\/mcp.O113.027342_bib30","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1021\/pr101060v","article-title":"More than 100,000 detectable peptide species elute in single shotgun proteomics runs but the majority is inaccessible to data-dependent LC-MS\/MS","volume":"10","author":"Michalski","year":"2011","journal-title":"J. Proteome Res"},{"key":"10.1074\/mcp.O113.027342_bib31","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1586\/14789450.4.1.13","article-title":"Quantitative proteomic approaches for studying phosphotyrosine signaling","volume":"4","author":"Ding","year":"2007","journal-title":"Expert Rev. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib32","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1074\/mcp.M400118-MCP200","article-title":"Phosphotyrosine signaling networks in epidermal growth factor receptor overexpressing squamous carcinoma cells","volume":"4","author":"Thelemann","year":"2005","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib33","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1021\/pr1006203","article-title":"Mass spectrometry mapping of epidermal growth factor receptor phosphorylation related to oncogenic mutations and tyrosine kinase inhibitor sensitivity","volume":"10","author":"Zhang","year":"2011","journal-title":"J. Proteome Res"},{"key":"10.1074\/mcp.O113.027342_bib34","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1016\/j.jprot.2011.11.004","article-title":"Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells","volume":"75","author":"Weber","year":"2012","journal-title":"J. Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib35","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1124\/dmd.105.007765","article-title":"Metabolism and excretion of erlotinib, a small molecule inhibitor of epidermal growth factor receptor tyrosine kinase, in healthy male volunteers","volume":"34","author":"Ling","year":"2006","journal-title":"Drug Metab. Dispos"},{"key":"10.1074\/mcp.O113.027342_bib36","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.1038\/sj.onc.1207390","article-title":"Role of protein ubiquitylation in regulating endocytosis of receptor tyrosine kinases","volume":"23","author":"Marmor","year":"2004","journal-title":"Oncogene"},{"key":"10.1074\/mcp.O113.027342_bib37","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1128\/MCB.20.4.1448-1459.2000","article-title":"A novel positive feedback loop mediated by the docking protein Gab1 and phosphatidylinositol 3-kinase in epidermal growth factor receptor signaling","volume":"20","author":"Rodrigues","year":"2000","journal-title":"Mol. Cell. Biol"},{"key":"10.1074\/mcp.O113.027342_bib38","doi-asserted-by":"crossref","first-page":"24380","DOI":"10.1074\/jbc.M010275200","article-title":"Phosphotyrosines 627 and 659 of Gab1 constitute a bisphosphoryl tyrosine-based activation motif (BTAM) conferring binding and activation of SHP2","volume":"276","author":"Cunnick","year":"2001","journal-title":"J. Biol. Chem"},{"key":"10.1074\/mcp.O113.027342_bib39","doi-asserted-by":"crossref","first-page":"ra64","DOI":"10.1126\/scisignal.2000998","article-title":"Akt-RSK-S6 kinase signaling networks activated by oncogenic receptor tyrosine kinases","volume":"3","author":"Moritz","year":"2010","journal-title":"Sci. Signal"},{"key":"10.1074\/mcp.O113.027342_bib40","doi-asserted-by":"crossref","first-page":"4383","DOI":"10.1002\/pmic.200800204","article-title":"Quantitative proteomics and phosphoproteomics reveal novel insights into complexity and dynamics of the EGFR signaling network","volume":"8","author":"Morandell","year":"2008","journal-title":"Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/msb4100012","article-title":"Phosphotyrosine interactome of the ErbB-receptor kinase family","volume":"1","author":"Schulze","year":"2005","journal-title":"Mol. Syst. Biol"},{"key":"10.1074\/mcp.O113.027342_bib42","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/nature04177","article-title":"A quantitative protein interaction network for the ErbB receptors using protein microarrays","volume":"439","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"10.1074\/mcp.O113.027342_bib43","doi-asserted-by":"crossref","first-page":"20206","DOI":"10.1074\/jbc.M109.021139","article-title":"GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling","volume":"284","author":"Tashiro","year":"2009","journal-title":"J. Biol. Chem"},{"key":"10.1074\/mcp.O113.027342_bib44","doi-asserted-by":"crossref","first-page":"3503","DOI":"10.1038\/sj.onc.1210138","article-title":"c-Src modulates ErbB2 and ErbB3 heterocomplex formation and function","volume":"26","author":"Ishizawar","year":"2007","journal-title":"Oncogene"},{"key":"10.1074\/mcp.O113.027342_bib45","doi-asserted-by":"crossref","first-page":"5858","DOI":"10.1128\/MCB.01731-08","article-title":"c-Src associates with ErbB2 through an interaction between catalytic domains and confers enhanced transforming potential","volume":"29","author":"Marcotte","year":"2009","journal-title":"Mol. Cell. Biol"},{"key":"10.1074\/mcp.O113.027342_bib46","doi-asserted-by":"crossref","first-page":"4163","DOI":"10.1038\/onc.2011.130","article-title":"Phosphoproteomic mass spectrometry profiling links Src family kinases to escape from HER2 tyrosine kinase inhibition","volume":"30","author":"Rexer","year":"2011","journal-title":"Oncogene"},{"key":"10.1074\/mcp.O113.027342_bib47","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1038\/35052073","article-title":"Untangling the ErbB signalling network","volume":"2","author":"Yarden","year":"2001","journal-title":"Nat. Rev. Mol. Cell. Biol"},{"key":"10.1074\/mcp.O113.027342_bib48","doi-asserted-by":"crossref","first-page":"rs2","DOI":"10.1126\/scisignal.2002548","article-title":"Hyperplexing: a method for higher-order multiplexed quantitative proteomics provides a map of the dynamic response to rapamycin in yeast","volume":"5","author":"Dephoure","year":"2012","journal-title":"Sci. Signal"},{"key":"10.1074\/mcp.O113.027342_bib49","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1021\/pr800650r","article-title":"Temporal profiling of the adipocyte proteome during differentiation using a five-plex SILAC based strategy","volume":"8","author":"Molina","year":"2009","journal-title":"J. Proteome Res"},{"key":"10.1074\/mcp.O113.027342_bib50","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1002\/pmic.200800468","article-title":"Quantitative analysis of cell signaling and drug action via mass spectrometry-based systems level phosphoproteomics","volume":"9","author":"Tedford","year":"2009","journal-title":"Proteomics"},{"key":"10.1074\/mcp.O113.027342_bib51","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1038\/leu.2012.243","article-title":"Imatinib-dependent tyrosine phosphorylation profiling of Bcr-Abl-positive chronic myeloid leukemia cells","volume":"27","author":"Preisinger","year":"2013","journal-title":"Leukemia"},{"key":"10.1074\/mcp.O113.027342_bib52","doi-asserted-by":"crossref","first-page":"5860","DOI":"10.1073\/pnas.0608638104","article-title":"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks","volume":"104","author":"Wolf-Yadlin","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A"}],"container-title":["Molecular &amp; Cellular Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620346090?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620346090?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/mcp.O113.027342","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T01:01:48Z","timestamp":1761526908000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1535947620346090"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":52,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["S1535947620346090"],"URL":"https:\/\/doi.org\/10.1074\/mcp.o113.027342","relation":{},"ISSN":["1535-9476"],"issn-type":[{"value":"1535-9476","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a 5-plex SILAC Method Tuned for the Quantitation of Tyrosine Phosphorylation Dynamics","name":"articletitle","label":"Article Title"},{"value":"Molecular & Cellular Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/mcp.O113.027342","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2013 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}