{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T03:16:30Z","timestamp":1771384590486,"version":"3.50.1"},"reference-count":34,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T00:00:00Z","timestamp":1606867200000},"content-version":"vor","delay-in-days":305,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000048","name":"American Cancer Society","doi-asserted-by":"publisher","award":["130635-RSG-17\u2013005-01-CCE"],"award-info":[{"award-number":["130635-RSG-17\u2013005-01-CCE"]}],"id":[{"id":"10.13039\/100000048","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["PC151060"],"award-info":[{"award-number":["PC151060"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM122451"],"award-info":[{"award-number":["R35GM122451"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["mcponline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular &amp; Cellular Proteomics"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1074\/mcp.ra119.001785","type":"journal-article","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T13:55:32Z","timestamp":1575294932000},"page":"294-307","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"title":["Profiling the Surfaceome Identifies Therapeutic Targets for Cells with Hyperactive mTORC1 Signaling"],"prefix":"10.1016","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9733-803X","authenticated-orcid":false,"given":"Junnian","family":"Wei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Leung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Truillet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Ruggero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Wells","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4947-1316","authenticated-orcid":false,"given":"Michael J.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1074\/mcp.RA119.001785_bib1","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1038\/nrd3531","article-title":"Rapamycin passes the torch: a new generation of mTOR inhibitors","volume":"10","author":"Benjamin","year":"2011","journal-title":"Nat. Rev. Drug Discovery"},{"key":"10.1074\/mcp.RA119.001785_bib2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1158\/2159-8290.CD-12-0018","article-title":"Negative feedback and adaptive resistance to the targeted therapy of cancer","volume":"2","author":"Chandarlapaty","year":"2012","journal-title":"Cancer Discovery"},{"key":"10.1074\/mcp.RA119.001785_bib3","first-page":"15","article-title":"mTOR inhibitors at a glance","volume":"7","author":"Zheng","year":"2015","journal-title":"Mol. Cell. Pharmacol"},{"key":"10.1074\/mcp.RA119.001785_bib4","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.cell.2017.02.004","article-title":"mTOR signaling in growth, metabolism, and disease","volume":"168","author":"Saxton","year":"2017","journal-title":"Cell"},{"key":"10.1074\/mcp.RA119.001785_bib5","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/j.ccr.2007.04.021","article-title":"Identification of the JNK signaling pathway as a functional target of the tumor suppressor PTEN","volume":"11","author":"Vivanco","year":"2007","journal-title":"Cancer Cell"},{"key":"10.1074\/mcp.RA119.001785_bib6","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1093\/hmg\/7.6.1053","article-title":"Interaction between hamartin and tuberin, the TSC1 and TSC2 gene products","volume":"7","author":"van Slegtenhorst","year":"1998","journal-title":"Human Mol. Gen"},{"key":"10.1074\/mcp.RA119.001785_bib7","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1038\/ncb999","article-title":"Rheb is a direct target of the tuberous sclerosis tumour suppressor proteins","volume":"5","author":"Zhang","year":"2003","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/mcp.RA119.001785_bib8","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1042\/BJ20080281","article-title":"The TSC1-TSC2 complex: a molecular switchboard controlling cell growth","volume":"412","author":"Huang","year":"2008","journal-title":"Biochem. J"},{"key":"10.1074\/mcp.RA119.001785_bib9","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nmeth.3047","article-title":"Improved vectors and genome-wide libraries for CRISPR screening","volume":"11","author":"Sanjana","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1074\/mcp.RA119.001785_bib10","doi-asserted-by":"crossref","first-page":"W272","DOI":"10.1093\/nar\/gkw398","article-title":"CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering","volume":"44","author":"Labun","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"10.1074\/mcp.RA119.001785_bib11","doi-asserted-by":"crossref","first-page":"2650","DOI":"10.1038\/nprot.2006.427","article-title":"A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)","volume":"1","author":"Ong","year":"2006","journal-title":"Nat. Protocols"},{"key":"10.1074\/mcp.RA119.001785_bib12","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1038\/nbt.1532","article-title":"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins","volume":"27","author":"Wollscheid","year":"2009","journal-title":"Nat. Biotechnol"},{"key":"10.1074\/mcp.RA119.001785_bib13","doi-asserted-by":"crossref","first-page":"3045","DOI":"10.1158\/1078-0432.CCR-16-2448","article-title":"Noninvasive measurement of mTORC1 signaling with 89Zr-transferrin","volume":"23","author":"Truillet","year":"2017","journal-title":"Clin. Cancer Res"},{"key":"10.1074\/mcp.RA119.001785_bib14","doi-asserted-by":"crossref","first-page":"R142","DOI":"10.1186\/gb-2007-8-7-r142","article-title":"siRNA screen of the human signaling proteome identifies the PtdIns(3,4,5)P3-mTOR signaling pathway as a primary regulator of transferrin uptake","volume":"8","author":"Galvez","year":"2007","journal-title":"Genome Biol"},{"key":"10.1074\/mcp.RA119.001785_bib15","doi-asserted-by":"crossref","first-page":"C836","DOI":"10.1152\/ajpcell.00554.2007","article-title":"A GSK-3\/TSC2\/mTOR pathway regulates glucose uptake and GLUT1 glucose transporter expression","volume":"295","author":"Buller","year":"2008","journal-title":"Am. J. Physiol. Cell Physiol"},{"key":"10.1074\/mcp.RA119.001785_bib16","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1038\/35010550","article-title":"The TSC1 tumour suppressor hamartin regulates cell adhesion through ERM proteins and the GTPase Rho","volume":"2","author":"Lamb","year":"2000","journal-title":"Nat. Cell Biol"},{"key":"10.1074\/mcp.RA119.001785_bib17","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.jacc.2016.04.060","article-title":"A Test in Context: Neprilysin: Function, Inhibition, and Biomarker","volume":"68","author":"Bayes-Genis","year":"2016","journal-title":"J. Am. College Cardiol"},{"key":"10.1074\/mcp.RA119.001785_bib18","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.molmed.2008.06.003","article-title":"The moonlighting enzyme CD13: old and new functions to target","volume":"14","author":"Mina-Osorio","year":"2008","journal-title":"Trends Mol. Med"},{"key":"10.1074\/mcp.RA119.001785_bib19","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.molcel.2010.06.022","article-title":"Activation of a metabolic gene regulatory network downstream of mTOR complex 1","volume":"39","author":"Duvel","year":"2010","journal-title":"Mol. Cell"},{"key":"10.1074\/mcp.RA119.001785_bib20","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1038\/s41591-018-0139-y","article-title":"Genetically engineered human cortical spheroid models of tuberous sclerosis","volume":"24","author":"Blair","year":"2018","journal-title":"Nat. Med"},{"key":"10.1074\/mcp.RA119.001785_bib21","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1517\/13543784.2014.864276","article-title":"Tosedostat for the treatment of relapsed and refractory acute myeloid leukemia","volume":"23","author":"DiNardo","year":"2014","journal-title":"Expert Opinion Investigational Drugs"},{"key":"10.1074\/mcp.RA119.001785_bib22","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/S1470-2045(13)70037-8","article-title":"Two dosing regimens of tosedostat in elderly patients with relapsed or refractory acute myeloid leukaemia (OPAL): a randomised open-label phase 2 study","volume":"14","author":"Cortes","year":"2013","journal-title":"Lancet Oncol"},{"key":"10.1074\/mcp.RA119.001785_bib23","doi-asserted-by":"crossref","first-page":"6669","DOI":"10.1158\/0008-5472.CAN-07-6627","article-title":"CHR-2797: an antiproliferative aminopeptidase inhibitor that leads to amino acid deprivation in human leukemic cells","volume":"68","author":"Krige","year":"2008","journal-title":"Cancer Res"},{"key":"10.1074\/mcp.RA119.001785_bib24","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1126\/science.1226344","article-title":"Genome sequencing identifies a basis for everolimus sensitivity","volume":"338","author":"Iyer","year":"2012","journal-title":"Science"},{"key":"10.1074\/mcp.RA119.001785_bib25","doi-asserted-by":"crossref","first-page":"3242","DOI":"10.1038\/emboj.2011.257","article-title":"Regulation of TFEB and V-ATPases by mTORC1","volume":"30","author":"Pena-Llopis","year":"2011","journal-title":"EMBO J"},{"key":"10.1074\/mcp.RA119.001785_bib26","doi-asserted-by":"crossref","first-page":"15816","DOI":"10.1038\/ncomms15816","article-title":"The genomic landscape of tuberous sclerosis complex","volume":"8","author":"Martin","year":"2017","journal-title":"Nat. Commun"},{"key":"10.1074\/mcp.RA119.001785_bib27","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1182\/blood-2002-05-1422","article-title":"The angiogenic regulator CD13\/APN is a transcriptional target of Ras signaling pathways in endothelial morphogenesis","volume":"101","author":"Bhagwat","year":"2003","journal-title":"Blood"},{"key":"10.1074\/mcp.RA119.001785_bib28","doi-asserted-by":"crossref","first-page":"49358","DOI":"10.1074\/jbc.M308071200","article-title":"CD13\/APN transcription is induced by RAS\/MAPK-mediated phosphorylation of Ets-2 in activated endothelial cells","volume":"278","author":"Petrovic","year":"2003","journal-title":"J. Biol. Chem"},{"key":"10.1074\/mcp.RA119.001785_bib29","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1111\/j.1349-7006.2010.01826.x","article-title":"Aminopeptidase N (CD13) as a target for cancer chemotherapy","volume":"102","author":"Wickstrom","year":"2011","journal-title":"Cancer Sci"},{"key":"10.1074\/mcp.RA119.001785_bib30","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.31098","article-title":"Targeting RAS-driven human cancer cells with antibodies to upregulated and essential cell-surface proteins","volume":"7","author":"Martinko","year":"2018","journal-title":"eLife"},{"key":"10.1074\/mcp.RA119.001785_bib31","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S1535-6108(03)00331-3","article-title":"Synergy in tumor suppression by direct interaction of neutral endopeptidase with PTEN","volume":"5","author":"Sumitomo","year":"2004","journal-title":"Cancer Cell"},{"key":"10.1074\/mcp.RA119.001785_bib32","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1158\/0008-5472.CAN-05-2925","article-title":"mTOR inhibition induces upstream receptor tyrosine kinase signaling and activates Akt","volume":"66","author":"O'Reilly","year":"2006","journal-title":"Cancer Res"},{"key":"10.1074\/mcp.RA119.001785_bib33","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1158\/0008-5472.CAN-16-1567","article-title":"Menin and Daxx Interact to Suppress Neuroendocrine Tumors through Epigenetic Control of the Membrane Metallo-Endopeptidase","volume":"77","author":"Feng","year":"2017","journal-title":"Cancer Res"},{"key":"10.1074\/mcp.RA119.001785_bib34","doi-asserted-by":"crossref","first-page":"13355","DOI":"10.1007\/s13277-016-5248-y","article-title":"Neutral endopeptidase (NEP) is differentially involved in biological activities and cell signaling of colon cancer cell lines derived from various stages of tumor development","volume":"37","author":"Mizerska-Kowalska","year":"2016","journal-title":"Tumour Biol"}],"container-title":["Molecular &amp; Cellular Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620350805?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947620350805?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1074\/mcp.RA119.001785","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T01:07:15Z","timestamp":1761527235000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1535947620350805"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["S1535947620350805"],"URL":"https:\/\/doi.org\/10.1074\/mcp.ra119.001785","relation":{},"ISSN":["1535-9476"],"issn-type":[{"value":"1535-9476","type":"print"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Profiling the Surfaceome Identifies Therapeutic Targets for Cells with Hyperactive mTORC1 Signaling","name":"articletitle","label":"Article Title"},{"value":"Molecular & Cellular Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1074\/mcp.RA119.001785","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Wei et al.","name":"copyright","label":"Copyright"}]}}