{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T01:25:18Z","timestamp":1760318718776},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2007,7,24]],"date-time":"2007-07-24T00:00:00Z","timestamp":1185235200000},"content-version":"unspecified","delay-in-days":9276,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[1982,3]]},"abstract":"<jats:p>1. Experiments were undertaken to examine the errors associated with the use of indigestible markers, the <jats:sup>51<\/jats:sup>Cr-labelled complex of chromium ethylenediaminetetra-acetic acid (<jats:sup>51<\/jats:sup>Cr-EDTA) and <jats:sup>103<\/jats:sup>Ru-labelled tris-(1,10-phenanthroline)-ruthenium(II) chloride (<jats:sup>103<\/jats:sup>Ru-P), to measure liquid- and solid-phase digesta kinetics in the caecum and proximal colon of sheep.<\/jats:p><jats:p>2. First-order kinetics of markers were observed following either single injection or termination of continuous infusion. There were no differences between the half-times (T<jats:sub>\u00bd<\/jats:sub>) of marker in the caecum plus proximal colon whether calculated from marker concentration in caecal digesta or in faeces. There were also no differences in the <jats:italic>T<\/jats:italic><jats:sub>\u00bd<\/jats:sub> values calculated for the liquid- and solid-phase markers. When pool sizes calculated from the marker kinetics were compared with the volume of digesta present in the caecum and proximal colon at slaughter, it appeared that the <jats:sup>51<\/jats:sup>Cr-EDTA and <jats:sup>103<\/jats:sup>Ru-P caecal pools described the digesta contained in the entire caecum and proximal colon.<\/jats:p><jats:p>3. The flow-rates of dry matter (DM) through the caecum of sheep given 694 g lucerne (<jats:italic>Medicago sativa<\/jats:italic>) DM\/d were similar whether estimated from total collection of faeces, by single injection of marker, or by the ratio, marker concentration: DM in either caecal digesta or faeces during continuous infusion of marker into either the rumen or the caecum.<\/jats:p><jats:p>4. In sheep given 553 g brome grass (<jats:italic>Bromus inermus<\/jats:italic>) DM\/d the coefficient of variation of estimates of the plateau of <jats:sup>51<\/jats:sup>Cr-EDTA marker during continuous infusion into the caecum was greater when 130 ml infusate\/d were administered than with 1000 ml\/d.<\/jats:p><jats:p>5. In the sheep given brome grass the lines of best fit of decline in In <jats:sup>51<\/jats:sup>Cr-EDTA marker concentration <jats:italic>v<\/jats:italic>. time following termination of the continuous infusions described previously and following single injection of marker in 20 or 2 ml into the caecum were examined. The variation was least when 1000 ml infusate\/d had been administered and was unacceptably large following a single injection of 2 ml.<\/jats:p><jats:p>6. These experiments showed that tracer techniques could provide unbiased estimates of trace kinetics in the caecum and proximal colon.<\/jats:p>","DOI":"10.1079\/bjn19820039","type":"journal-article","created":{"date-parts":[[2005,5,24]],"date-time":"2005-05-24T23:20:35Z","timestamp":1116976835000},"page":"301-309","source":"Crossref","is-referenced-by-count":11,"title":["Studies of the large intestine of sheep"],"prefix":"10.1017","volume":"47","author":[{"given":"R. M.","family":"Dixon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. V.","family":"Nolan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. P.","family":"Milligan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2007,7,24]]},"reference":[{"key":"S0007114582001172_ref010","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19730029"},{"key":"S0007114582001172_ref006","doi-asserted-by":"publisher","DOI":"10.2527\/jas1976.4261465x"},{"key":"S0007114582001172_ref002","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19650031"},{"key":"S0007114582001172_ref015","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19760016"},{"key":"S0007114582001172_ref020","first-page":"149","volume":"33","author":"Ulyatt","year":"1973","journal-title":"Proc. N.Z. Soc. Anim. Prod."},{"key":"S0007114582001172_ref021","volume-title":"Digestion and Metabolism in the Ruminant","author":"Ulyatt","year":"1975"},{"key":"S0007114582001172_ref004","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19640015"},{"key":"S0007114582001172_ref007","first-page":"1180","volume":"36","author":"Glinsky","year":"1977","journal-title":"Fedn Proc. Fedn Am. Socs exp. Biol."},{"key":"S0007114582001172_ref016","unstructured":"Rowe J. B. (1978). Studies on the interrelationships between fermentation, digestion and metabolism in sheep. PhD thesis, University of New England, Armidale, Australia."},{"key":"S0007114582001172_ref011","doi-asserted-by":"publisher","DOI":"10.1017\/S0021859600023601"},{"key":"S0007114582001172_ref018","volume-title":"Statistical Methods","author":"Snedecor","year":"1967"},{"key":"S0007114582001172_ref012","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19600021"},{"key":"S0007114582001172_ref019","doi-asserted-by":"publisher","DOI":"10.1016\/0020-708X(71)90004-4"},{"key":"S0007114582001172_ref001","volume-title":"Progress in Nutrition and Allied Sciences","author":"Ash","year":"1963"},{"key":"S0007114582001172_ref013","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/S0034-5288(18)33942-0","volume":"14","author":"MacRae","year":"1973","journal-title":"Res. vet. Sci."},{"key":"S0007114582001172_ref014","volume":"37","author":"MacRae","year":"1978","journal-title":"Proc. Nutr. Soc."},{"key":"S0007114582001172_ref009","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19730028"},{"key":"S0007114582001172_ref005","doi-asserted-by":"publisher","DOI":"10.1071\/AR9690491"},{"key":"S0007114582001172_ref017","first-page":"173","volume-title":"Tracer methods for in vivo kinetics","author":"Shipley","year":"1972"},{"key":"S0007114582001172_ref003","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19820038"},{"key":"S0007114582001172_ref008","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1965.sp007531"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114582001172","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T21:39:03Z","timestamp":1558733943000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114582001172\/type\/journal_article"}},"subtitle":["2. Kinetics of liquid and solid phase markers in the caecum and proximal colon"],"short-title":[],"issued":{"date-parts":[[1982,3]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1982,3]]}},"alternative-id":["S0007114582001172"],"URL":"https:\/\/doi.org\/10.1079\/bjn19820039","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,3]]}}}