{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:04:28Z","timestamp":1760645068600},"reference-count":24,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T00:00:00Z","timestamp":1173398400000},"content-version":"unspecified","delay-in-days":6825,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[1988,7]]},"abstract":"<jats:p>1. The effect of unsaturated long-chain non-esterified fatty acids (NEFA) on the human leucocyte sodium-pump was studied in vivo.<\/jats:p><jats:p>2. Plasma NEFA level was raised acutely from 0\u00b728 (<jats:sc>sd<\/jats:sc>0\u00b710) to 2\u00b754 (<jats:sc>sd<\/jats:sc>0\u00b748) mmol\/l by infusion of \u2018Intralipid 20%\u2019 (trademark) at 90 ml\/h with heparin, and the human leucocyte<jats:sup>22<\/jats:sup>Na efflux rate constants were studied in eight normal weight males.<\/jats:p><jats:p>3. After 3 h, there was a significant lowering of the total (from 3\u00b797 (<jats:sc>sd<\/jats:sc>0\u00b792) to 3\u00b710 (<jats:sc>sd<\/jats:sc>0\u00b771)\/h;<jats:italic>P<\/jats:italic>&lt; 0\u00b701) and ouabain-sensitive<jats:sup>22<\/jats:sup>Na efflux rate constants (from 2\u00b789 (<jats:sc>sd<\/jats:sc>0\u00b755) to 2\u00b737 (<jats:sc>sd<\/jats:sc>0\u00b762)\/h;<jats:italic>P<\/jats:italic>&lt; 0\u00b702). Ouabain-insensitive efflux rate constants showed a tendency to fall (from 1\u00b708 (<jats:sc>sd<\/jats:sc>0\u00b751) to 0\u00b773 (<jats:sc>sd<\/jats:sc>0\u00b723)\/h). Leucocyte potassium content remained unchanged, but sodium content rose from 31 (<jats:sc>sd<\/jats:sc>12) to 38 (<jats:sc>sd<\/jats:sc>18) mmol\/kg dry weight (<jats:italic>P<\/jats:italic>&lt; 0\u00b705). Total, ouabain-insensitive and ouabain-sensitive efflux rates did not change significantly during the Intralipid-heparin infusion.<\/jats:p><jats:p>4. Plasma insulin levels rose gradually throughout the 3 h infusion period.<\/jats:p><jats:p>5. In conclusion, NEFA, when raised to pathological levels, can inhibit the leucocyte Na-pump in vivo even in the presence of physiological levels of serum albumin, and may increase insulin secretion.<\/jats:p>","DOI":"10.1079\/bjn19880075","type":"journal-article","created":{"date-parts":[[2005,6,9]],"date-time":"2005-06-09T07:49:51Z","timestamp":1118303391000},"page":"49-55","source":"Crossref","is-referenced-by-count":2,"title":["The effect of Intralipid<sup>\u00ae<\/sup>infusion on the human leucocyte sodium-pump in vivo"],"prefix":"10.1017","volume":"60","author":[{"given":"L. L.","family":"Ng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. D. 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