{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:53:49Z","timestamp":1762520029069},"reference-count":39,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T00:00:00Z","timestamp":1173398400000},"content-version":"unspecified","delay-in-days":6582,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[1989,3]]},"abstract":"<jats:p>1. A number of dietary sugars are known to mediate the effects of copper deficiency. The effects of lactose (compared with sucrose) and a dietary Cu deficiency on hepatic and cardiac antioxidant enzyme activities and tissue mineral element status were investigated in the rat.<\/jats:p><jats:p>2. Groups (<jats:italic>n<\/jats:italic>6) of male weanling Wistar rats were provided<jats:italic>ad lib.<\/jats:italic>with deionized water and diets containing sucrose (580 g\/kg) or sucrose and lactose (387 g\/kg and 193 g\/kg respectively) with either control (12.0 mg\/kg) or deficient (1.5 mg\/kg) quantities of Cu for 77 d.<\/jats:p><jats:p>3. Animals consuming the low-Cu diets exhibited significantly decreased tissue Cu levels (<jats:italic>P<\/jats:italic>&lt; 0.01), hepatic and cardiac cytochrome<jats:italic>c<\/jats:italic>oxidase (<jats:italic>EC<\/jats:italic>1.9.3.1, CCO) activities (<jats:italic>P<\/jats:italic>&lt; 0.01 and<jats:italic>P<\/jats:italic>&lt; 0.001 respectively) and hepatic Cu-zinc superoxide dismutase (<jats:italic>EC<\/jats:italic>1.15.1.1, CuZnSOD) activity (<jats:italic>P<\/jats:italic>&lt; 0.05). The low-Cu diets also significantly decreased cardiac manganese superoxide dismutase (<jats:italic>EC<\/jats:italic>1.15.1.1, MnSOD), catalase (<jats:italic>EC<\/jats:italic>1.11.1.6) and glutathione peroxidase (<jats:italic>EC<\/jats:italic>1.11.1.9, GSH-Px) activities (<jats:italic>P<\/jats:italic>&lt; 0.01,<jats:italic>P<\/jats:italic>&lt; 0.05 and<jats:italic>P<\/jats:italic>&lt; 0.001 respectively).<\/jats:p><jats:p>4. Hepatic Mn was significantly increased in both lactose-fed (<jats:italic>P<\/jats:italic>&lt; 0.001) and Cu-deficient (<jats:italic>P<\/jats:italic>&lt; 0.01) animals. These increases were unrelated to hepatic MnSOD activity. Cardiac Zn was significantly (<jats:italic>P<\/jats:italic>&lt; 0.01) increased in Cu-deficient animals.<\/jats:p><jats:p>5. Lactose feeding resulted in significantly increased cardiac CCO activity (<jats:italic>P<\/jats:italic>&lt; 0.001) but significantly decreased hepatic CuZnSOD (<jats:italic>P<\/jats:italic>&lt; 0.05), catalase (<jats:italic>P<\/jats:italic>&lt; 0.01) and GSH-Px (<jats:italic>P<\/jats:italic>&lt; 0.001) activities.<\/jats:p><jats:p>6. The activities of lactose dehydrogenase (<jats:italic>EC<\/jats:italic>1.1.1.27, LDH) and glucose-6-phosphate dehydrogenase (<jats:italic>EC<\/jats:italic>1.1.1.49, G6PDH) were found to be significantly (<jats:italic>P<\/jats:italic>&lt; 0.05 and<jats:italic>P<\/jats:italic>&lt; 0.01 respectively) increased in Cu-deficient animals and G6PDH activity was significantly (<jats:italic>P<\/jats:italic>&lt; 0.01) decreased as a result of lactose consumption.<\/jats:p><jats:p>7. The observed changes in antioxidant enzyme activities associated with both Cu deficieny and lactose consumption may have important implications for the development of free radical mediated cell damage. However, no significant differences in either hepatic or cardiac levels of thiobarbituric acid reactive substances, a measure of lipid peroxidation, were found.<\/jats:p>","DOI":"10.1079\/bjn19890122","type":"journal-article","created":{"date-parts":[[2005,9,17]],"date-time":"2005-09-17T04:07:20Z","timestamp":1126930040000},"page":"345-354","source":"Crossref","is-referenced-by-count":29,"title":["Effects of copper deficiency on hepatic and cardiac antioxidant enzyme activities in lactose- and sucrose-fed rats"],"prefix":"10.1017","volume":"61","author":[{"given":"S. M.","family":"Lynch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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