{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T09:34:54Z","timestamp":1772012094033,"version":"3.50.1"},"reference-count":45,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T00:00:00Z","timestamp":1173398400000},"content-version":"unspecified","delay-in-days":5852,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:p>The present work was undertaken to study the microbial activity in various segments of the gastrointestinal (GI) tract of pigs as influenced by the source and level of wheat and oat dietary fibre (DF). Eight experimental diets were prepared from wheat and oat fractions and studied in a series of two experiments using wheat flour as the DF-depleted control. The diets in Expt 1 were based on wheat flour and three iso-DF enriched diets comprising fractions rich in wheat aleurone, pericarp\/testa or bran. In Expt 2, oat bran was added to wheat flour to achieve the same DF intake level as in Expt 1. This series included further diets based on rolled oats and rolled oats plus oat bran. The eight diets were given to thirty-two ileal-cannulated pigs, with sixteen pigs in each experiment. After a total period of 34 d (Expt 1) and 42 d (Expt 2), the pigs were slaughtered 4 h post-feeding and samples taken for adenine nucleotides (adenosine 5'-triphosphate (ATP); adenylate energy charge (AEC)), organic acids (lactic acid (LA); short chain fatty acids (SCFA)) and pH at twelve sites of the GI tract. The microbial activity as measured by the ATP concentration was low in the stomach and the cranial two-thirds of the small intestine, but tended to increase in the distal third. In the caecum a sharp rise in microbial activity was observed; the highest level was found for the diet providing most fermentable substrates. In all the diets but the rolled oats+oat bran diets, microbial activity showed a descending pattern as the digesta moved through the colon. In the large intestine source and level of residues had a marked influence on microbial activity. LA was the chief organic acid in the stomach and small intestine (10\u201340 mmol\/l) while LA relative to SCFA was a minor component in the caecum and colon (10\u201320 mmol\/l). The contribution of SCFA to total organic acids was reciprocal to LA, i. e. low in the stomach and small intestine (&lt;20 mmol\/l) and high in the caecum and colon. In the large intestine the concentration of SCFA decreased from 100\u2013140 mmol\/l in the caecum and proximal colon to 40\u201380 mmol\/l in the distal colon. The acetic: propionic acid ratio increased from the caecum to the distal colon. With the diets based on oat alone (rolled oats; rolled oats+oat bran) the increase was less significant. DF addition and oats in particular increased the butyric acid molar ratio, from 0.06\u20130.08 for the wheat flour diet to 0.10\u20130.12 for the diet based on rolled oats+oat bran. For the same two diets the proportion of isobutyric and isovaleric acids increased more rapidly with the wheat-flour diet compared with the rolled oats+oat bran diet.<\/jats:p>","DOI":"10.1079\/bjn19910083","type":"journal-article","created":{"date-parts":[[2005,6,9]],"date-time":"2005-06-09T07:49:51Z","timestamp":1118303391000},"page":"233-248","source":"Crossref","is-referenced-by-count":152,"title":["Gastrointestinal implications in pigs of wheat and oat fractions"],"prefix":"10.1017","volume":"65","author":[{"given":"K. E.","family":"Bach Knudsen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Borg Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. O.","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inge","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2007,3,9]]},"reference":[{"key":"S0007114591000296_ref045","first-page":"243","article-title":"Adenosine triphosphate in the bovine rumen during maximum nutrient supply and starvation","volume":"41","author":"Wolstrup","year":"1976","journal-title":"Journal of Bacteriology"},{"key":"S0007114591000296_ref044","first-page":"134","volume-title":"Digestion and Metabolism in the Ruminant","author":"Wolin","year":"1975"},{"key":"S0007114591000296_ref043","doi-asserted-by":"publisher","DOI":"10.1017\/S0021859600054289"},{"key":"S0007114591000296_ref042","first-page":"495","article-title":"Faecal pH value and its modification by dietary means in South African black and white schoolchildren","volume":"24","author":"Walker","year":"1979","journal-title":"South African Medical Journal"},{"key":"S0007114591000296_ref041","first-page":"30","article-title":"Technical aspects of the bioluminescent firefly luciferase assay of ATP","volume":"26","author":"Thore","year":"1979","journal-title":"Science Tools"},{"key":"S0007114591000296_ref040","volume-title":"Statistical Methods","author":"Snedecor","year":"1973"},{"key":"S0007114591000296_ref039","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.31.100177.000543"},{"key":"S0007114591000296_ref038","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1984.sp002850"},{"key":"S0007114591000296_ref037","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/0016-5085(79)90217-8","article-title":"Effect of various diets on colonic growth in rats","volume":"77","author":"Ryan","year":"1979","journal-title":"Gastroenterology"},{"key":"S0007114591000296_ref035","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1128\/AEM.46.3.533-538.1983","article-title":"Relation of rumen ATP concentration to bacterial and protozoal numbers","volume":"46","author":"Nuzback","year":"1983","journal-title":"Applied and Environmental Microbiology"},{"key":"S0007114591000296_ref034","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/32.1.164"},{"key":"S0007114591000296_ref031","first-page":"1647","article-title":"Protein degradation by human intestinal bacteria","volume":"132","author":"Macfarlane","year":"1986","journal-title":"Journal of General Microbiology"},{"key":"S0007114591000296_ref027","doi-asserted-by":"publisher","DOI":"10.2527\/jas1974.39157x"},{"key":"S0007114591000296_ref025","doi-asserted-by":"publisher","DOI":"10.2527\/jas1978.472467x"},{"key":"S0007114591000296_ref023","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/116.2.242"},{"key":"S0007114591000296_ref022","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.1960.tb00197.x"},{"key":"S0007114591000296_ref021","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1128\/AEM.33.3.528-537.1977","article-title":"Use of adenosine 5'-triphosphate as an indicator of the microbiota biomass in rumen contents","volume":"33","author":"Forsberg","year":"1977","journal-title":"Applied and Environmental Microbiology"},{"key":"S0007114591000296_ref020","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(81)82623-9"},{"key":"S0007114591000296_ref019","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(79)83237-3"},{"key":"S0007114591000296_ref018","first-page":"492","article-title":"Study of the gastro-intestinal microflora of suckling piglets and early weaned piglets reared using different feeding systems. 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Microbial activity in the gastrointestinal tract"],"short-title":[],"issued":{"date-parts":[[1991,3]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,3]]}},"alternative-id":["S0007114591000296"],"URL":"https:\/\/doi.org\/10.1079\/bjn19910083","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,3]]}}}