{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:19:21Z","timestamp":1767676761019},"reference-count":17,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T00:00:00Z","timestamp":1173398400000},"content-version":"unspecified","delay-in-days":3507,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[1997,8]]},"abstract":"<jats:p><jats:italic>Medicago sativa<\/jats:italic>(lucerne) is used as a traditional plant treatment of diabetes. In the present study, administration of lucerne in the diet (62\u00b75 g\/kg) and drinking water (2\u00b75 g\/l) reduced the hyperglycaemia of streptozotocin-diabetic mice. An aqueous extract of lucerne (1 mg\/ml) stimulated 2-deoxy-glucose transport (1\u00b78-fold), glucose oxidation (1\u00b77-fold) and incorporation of glucose into glycogen (l\u00b76-fold) in mouse abdominal muscle. In acute 20 min tests, 0\u00b725\u20131 mg\/ml aqueous extract of lucerne evoked a stepwise 2\u00b75\u20136\u00b73-fold stimulation of insulin secretion from the BRIN-BD11 pancreatic B-cell line. This effect was abolished by 0\u00b75 mM-diazoxide, and prior exposure to extract did not affect subsequent stimulation of insulin secretion by 10 mM-L-alanine, thereby negating a detrimental effect on cell viability. The effect of extract was potentiated by 16\u00b77 mM-glucose and by 1 mM-3-isobutyl-1-methylxanthine. L-Alanine (10 mM) and a depolarizing concentration of KCI (25 mM) did not augment the insulin-releasing activity of lucerne. Activity of the extract was found to be heat stable and largely acetone insoluble, and was enhanced by exposure to acid and alkali (0\u00b71 M-HCI and NaOH) but decreased 25% with dialysis to remove components with molecular mass &lt;2000 Da. Sequential extraction with solvents revealed insulin-releasing activity in both methanol and water fractions indicating a cumulative effect of more than one extract constituent. The results demonstrate the presence of antihyperglycaemic, insulin-releasing and insulin-like activity in the traditional antidiabetic plant,<jats:italic>Medicago sativu<\/jats:italic>.<\/jats:p>","DOI":"10.1079\/bjn19970150","type":"journal-article","created":{"date-parts":[[2005,6,9]],"date-time":"2005-06-09T07:49:51Z","timestamp":1118303391000},"page":"325-334","source":"Crossref","is-referenced-by-count":67,"title":["Pancreatic and extra-pancreatic effects of the traditional anti-diabetic plant,<i>Medicago sativa<\/i>(lucerne)"],"prefix":"10.1017","volume":"78","author":[{"given":"Alison M","family":"Gray","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter R","family":"Flatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2007,3,9]]},"reference":[{"key":"S0007114597001372_ref017","first-page":"129","volume-title":"Frontiers of lnsulin Secretion and Pancreatic B-cell Research","author":"Yada","year":"1994"},{"key":"S0007114597001372_ref015","doi-asserted-by":"publisher","DOI":"10.1007\/BF00657506"},{"key":"S0007114597001372_ref008","first-page":"346","article-title":"Effect of metformin on peripheral insulin sensitivity in non-insulin dependent diabetes mellitus","volume":"12","author":"Prager","year":"1986","journal-title":"Diab\u00e8te Metabolisme"},{"key":"S0007114597001372_ref010","doi-asserted-by":"publisher","DOI":"10.1038\/194188a0"},{"key":"S0007114597001372_ref005","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1949.29.1.75"},{"key":"S0007114597001372_ref002","first-page":"212","article-title":"Effect of metformin on glucose metabolism in mouse soleus muscle","volume":"12","author":"Bailey","year":"1986","journal-title":"Diab\u00e8te et Metabolisme"},{"key":"S0007114597001372_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(62)91022-X"},{"key":"S0007114597001372_ref004","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1177\/026119298701500104","article-title":"A comparison of in vitro cytotoxicity assays and their application to water samples","volume":"15","author":"Hunt","year":"1987","journal-title":"Alternatives to Laboratory Animals"},{"key":"S0007114597001372_ref006","volume-title":"The Herb Book","author":"Lust","year":"1986"},{"key":"S0007114597001372_ref007","doi-asserted-by":"publisher","DOI":"10.2337\/diab.45.8.1132"},{"key":"S0007114597001372_ref003","doi-asserted-by":"publisher","DOI":"10.1007\/BF00252768"},{"key":"S0007114597001372_ref016","unstructured":"World Health Organization (1980). Second Report of the WHO Expert Committee on Diabetes Mellitus. Technical Report Series 646, p.66. Geneva: World Health Organization."},{"key":"S0007114597001372_ref014","doi-asserted-by":"publisher","DOI":"10.1007\/BF00405106"},{"key":"S0007114597001372_ref001","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.12.8.553"},{"key":"S0007114597001372_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(71)90332-9"},{"key":"S0007114597001372_ref012","doi-asserted-by":"publisher","DOI":"10.1007\/BF01223617"},{"key":"S0007114597001372_ref011","first-page":"161","volume-title":"Frontiers of Insulin Secretion and Pancreatic B-cell Research","author":"Schwanstecher","year":"1994"}],"container-title":["British Journal of Nutrition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0007114597001372","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,11]],"date-time":"2021-07-11T14:04:24Z","timestamp":1626012264000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0007114597001372\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,8]]}},"alternative-id":["S0007114597001372"],"URL":"https:\/\/doi.org\/10.1079\/bjn19970150","relation":{},"ISSN":["0007-1145","1475-2662"],"issn-type":[{"value":"0007-1145","type":"print"},{"value":"1475-2662","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8]]}}}