{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T23:15:30Z","timestamp":1768432530801,"version":"3.49.0"},"reference-count":13,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2007,3,9]],"date-time":"2007-03-09T00:00:00Z","timestamp":1173398400000},"content-version":"unspecified","delay-in-days":950,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p>The possibility of using exfoliated colonic epithelial cells for assessing the bioavailability of \u03b2-carotene was examined. Analysis of exfoliated colonic epithelial cells showed the presence of \u03b2-carotene and vitamin A. The \u03b2-carotene content was significantly lower in cells from stool samples of subjects on a \u03b2-carotene-poor diet than those receiving a single dose of a \u03b2-carotene supplement. Colonic epithelial cells isolated from stool samples collected daily during a wash-out period while the subjects were on a \u03b2-carotene-poor diet showed a steady decrease in \u03b2-carotene content, reaching the lowest value on day 7. Kinetic analysis showed that a single dose of a \u03b2-carotene supplement in the form of spirulina (<jats:italic>Spirulina platensis<\/jats:italic>) or agathi (<jats:italic>Sesbania grandiflora<\/jats:italic>) after the wash-out period caused an increase in the \u03b2-carotene content after a lag period of 5\u20137 d, but the vitamin A levels during these periods were not significantly affected. Analysis of plasma \u03b2-carotene concentration also showed similar changes, which correlated with those of exfoliated colonic cells. A relationship between the \u03b2-carotene content of the diet and that of the colonic epithelial cells suggests that analysis of the \u03b2-carotene content in exfoliated human colonic epithelial cells is a useful non-invasive method to assess the bioavailability of provitamin A \u03b2-carotene.<\/jats:p>","DOI":"10.1079\/bjn20041175","type":"journal-article","created":{"date-parts":[[2004,8,28]],"date-time":"2004-08-28T01:25:46Z","timestamp":1093656346000},"page":"241-245","source":"Crossref","is-referenced-by-count":21,"title":["Studies on the bioavailability of the provitamin A carotenoid, \u03b2-carotene, using human exfoliated colonic epithelial cells"],"prefix":"10.1017","volume":"92","author":[{"given":"T.","family":"Gireesh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. P.","family":"Nair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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