{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:43:14Z","timestamp":1790534594437,"version":"4.1.0"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2007,3,8]],"date-time":"2007-03-08T00:00:00Z","timestamp":1173312000000},"content-version":"unspecified","delay-in-days":584,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:p><jats:italic>Lactococcus lactis<\/jats:italic>is a commonly used starter strain that can be converted from a vitamin B<jats:sub>2<\/jats:sub>consumer into a vitamin B<jats:sub>2<\/jats:sub>\u2018factory\u2019 by over-expressing its riboflavin biosynthesis genes. The present study was conducted to assess in a rat bioassay the response of riboflavin produced by GM or native lactic acid bacteria (LAB). The riboflavin-producing strains were able to eliminate most physiological manifestations of ariboflavinosis such as stunted growth, elevated erythrocyte glutathione reductase activation coefficient values and hepatomegalia that were observed using a riboflavin depletion\u2013repletion model. Riboflavin status and growth rates were greatly improved when the depleted rats were fed with cultures of<jats:italic>L. lactis<\/jats:italic>that overproduced this vitamin whereas the native strain did not show the same effect. The present study is the first animal trial with food containing living bacteria that were engineered to overproduce riboflavin. These results pave the way for analysing the effect of similar riboflavin-overproducing LAB in human trials.<\/jats:p>","DOI":"10.1079\/bjn20051473","type":"journal-article","created":{"date-parts":[[2005,8,23]],"date-time":"2005-08-23T07:23:37Z","timestamp":1124781817000},"page":"262-267","source":"Crossref","is-referenced-by-count":41,"title":["<i>Lactococcus lactis<\/i>is capable of improving the riboflavin status in deficient rats"],"prefix":"10.1017","volume":"94","author":[{"given":"Jean Guy","family":"LeBlanc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Burgess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Sesma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela Savoy","family":"de Giori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douwe","family":"van Sinderen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2007,3,8]]},"reference":[{"key":"S0007114505001807_ref026","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19960133"},{"key":"S0007114505001807_ref025","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19950015"},{"key":"S0007114505001807_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(06)80022-4"},{"key":"S0007114505001807_ref023","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19880047"},{"key":"S0007114505001807_ref021","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19930055"},{"key":"S0007114505001807_ref028","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2001420"},{"key":"S0007114505001807_ref020","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/117.5.852"},{"key":"S0007114505001807_ref013","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1093\/clinchem\/46.8.1065","article-title":"Riboflavin as a determinant of plasma total homocysteine: effect modification by the methylenetetrahydrofolate reductase C677T polymorphism","volume":"46","author":"Hustad","year":"2000","journal-title":"Clin Chem"},{"key":"S0007114505001807_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00469-7"},{"key":"S0007114505001807_ref007","first-page":"53","article-title":"Comparison of changes in the uptake and mucosal processing of iron in riboflavin-deficient rats","volume":"30","author":"Butler","year":"1993","journal-title":"Biochem Mol Biol Int"},{"key":"S0007114505001807_ref005","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/58.1.85"},{"key":"S0007114505001807_ref002","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19970026"},{"key":"S0007114505001807_ref001","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19860097"},{"key":"S0007114505001807_ref009","first-page":"166","article-title":"Method for the detection of a biochemical riboflavin deficiency. 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