{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,6]],"date-time":"2023-05-06T22:41:03Z","timestamp":1683412863820},"reference-count":44,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2007,3,8]],"date-time":"2007-03-08T00:00:00Z","timestamp":1173312000000},"content-version":"unspecified","delay-in-days":341,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Nutr"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p>One of the most frequently reported immunomodulatory actions of<jats:italic>n<\/jats:italic>\u22123 PUFA is their ability to diminish<jats:italic>in vivo<\/jats:italic>lymphocyte proliferation. The purpose of this study was to determine if<jats:italic>n<\/jats:italic>\u22123 PUFA intake affects the kinetics or magnitude of the antigen-driven expansion of CD8<jats:sup>+<\/jats:sup>T-lymphocytes<jats:italic>in vivo<\/jats:italic>. In this study we utilized a well-characterized model of T-cell immunity (i.e. infection with the intracellular bacterium,<jats:italic>Listeria monocytogenes<\/jats:italic>). Weanling BALB\/c mice were fed one of two experimental diets that differed solely in fat source. Our control diet contained lard (180g\/kg) and was devoid of long-chain<jats:italic>n<\/jats:italic>\u22123 PUFA. The experimental diet contained 150g\/kg menhaden fish oil and 30g\/kg corn oil, thus providing approximately 8% of energy from long-chain<jats:italic>n<\/jats:italic>\u22123 PUFA. After 4 weeks, mice were infected intravenously with 10<jats:sup>6<\/jats:sup>colony-forming units of actA-deficient<jats:italic>L. monocytogenes<\/jats:italic>. Clonal expansion of antigen-specific CD8<jats:sup>+<\/jats:sup>T-cells in the spleen was measured at 5, 7, 9 and 14d post-challenge using a class I MHC tetramer loaded with the immunodominant peptide from this pathogen (i.e. K<jats:sup>d<\/jats:sup>:LLO<jats:sup>91\u201399<\/jats:sup>). We report that feeding mice a diet rich in<jats:italic>n<\/jats:italic>\u22123 fatty acids did not significantly impact either the kinetics or magnitude of<jats:italic>in vivo<\/jats:italic>, antigen-driven expansion of CD8<jats:sup>+<\/jats:sup>T-cells. Furthermore, contraction of this T-cell population was not affected by<jats:italic>n<\/jats:italic>\u22123 PUFA treatment. To our knowledge this is the first time MHC tetramers have been used to investigate the influence of<jats:italic>n<\/jats:italic>\u22123 PUFA on<jats:italic>in vivo<\/jats:italic>CD8<jats:sup>+<\/jats:sup>T-cell proliferation.<\/jats:p>","DOI":"10.1079\/bjn20051686","type":"journal-article","created":{"date-parts":[[2006,3,29]],"date-time":"2006-03-29T21:40:01Z","timestamp":1143668401000},"page":"838-844","source":"Crossref","is-referenced-by-count":6,"title":["<i>n<\/i>\u22123 PUFA fail to affect<i>in vivo<\/i>, antigen-driven CD8<sup>+<\/sup>T-cell proliferation in the spleen of na\u00efve mice"],"prefix":"10.1017","volume":"95","author":[{"given":"Robert","family":"Irons","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin L.","family":"Fritsche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2007,3,8]]},"reference":[{"key":"S0007114506001085_ref043","first-page":"603","article-title":"The effect of dietary lipid manipulation on rat lymphocyte subsets and proliferation","volume":"82","author":"Yaqoob","year":"1994","journal-title":"Immunology"},{"key":"S0007114506001085_ref041","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002821"},{"key":"S0007114506001085_ref038","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M400028-JLR200"},{"key":"S0007114506001085_ref037","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00474.x"},{"key":"S0007114506001085_ref036","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/123.11.1939"},{"key":"S0007114506001085_ref035","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2001.tb00533.x"},{"key":"S0007114506001085_ref033","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.11.3293"},{"key":"S0007114506001085_ref032","doi-asserted-by":"publisher","DOI":"10.1038\/353852a0"},{"key":"S0007114506001085_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2005.04.010"},{"key":"S0007114506001085_ref024","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/77.5.1287"},{"key":"S0007114506001085_ref023","first-page":"397","article-title":"Salmon diet and human immune status","volume":"46","author":"Kelley","year":"1992","journal-title":"Eur J Clin Nutr"},{"key":"S0007114506001085_ref021","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.23"},{"key":"S0007114506001085_ref020","doi-asserted-by":"publisher","DOI":"10.1007\/BF02522890"},{"key":"S0007114506001085_ref019","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.4.1163"},{"key":"S0007114506001085_ref018","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/122.6.1219"},{"key":"S0007114506001085_ref016","doi-asserted-by":"publisher","DOI":"10.1079\/PNS19980081"},{"key":"S0007114506001085_ref013","doi-asserted-by":"publisher","DOI":"10.1385\/IR:25:3:247"},{"key":"S0007114506001085_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(99)01549-2"},{"key":"S0007114506001085_ref010","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2000.tb01422.x"},{"key":"S0007114506001085_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80540-3"},{"key":"S0007114506001085_ref005","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.8.4063"},{"key":"S0007114506001085_ref027","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00231-3"},{"key":"S0007114506001085_ref004","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","article-title":"Adoptive transfer of immunity to Listeria monocytogenes. 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