{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:48:32Z","timestamp":1789573712881,"version":"build-2803163510"},"reference-count":45,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"unspecified","delay-in-days":7986,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The nematode <jats:italic>Anisakis simplex<\/jats:italic> causes anisakidiasis, a disease that often mimics other gastrointestinal diseases. Patients with digestive haemorrhaging, Crohn's disease, digestive cancer and appendicitis were analysed for antibodies to <jats:italic>A. simplex.<\/jats:italic> Antibody detection was carried out by enzyme-linked immunosorbent assay (ELISA) and immunoblotting using crude extract (CE) antigen and excretory-secretory (ES) products. Total immunoglobulin (Igs), IgG, IgM, IgA and IgE were studied. The highest percentage was obtained when Igs were tested against CE antigen. A higher percentage of positivity was observed with the appendicitis group. The Crohn's disease group showed the highest levels of IgG against the ES antigen. Using immunoblotting, 24% and 48% of sera from patients with symptoms of Crohn's disease and digestive haemorrhaging, respectively, showed a positive immunorecognition pattern of CE antigen. The prevalence of detectable antibodies against <jats:italic>A. simplex<\/jats:italic> is higher in patients with digestive disorders than in the healthy population. A linear correlation was observed between prothrombin activity and Igs-CE, IgA-CE and IgA-ES but not between IgE-CE and the other immunoglobulin levels. Specific IgA is associated with a higher activity index of Crohn's disease. Specific antibodies were observed against <jats:italic>A. simplex<\/jats:italic> in patients with appendicitis and gastrointestinal cancer, indicating a higher rate of positivity for IgA.<\/jats:p>","DOI":"10.1079\/joh2001104","type":"journal-article","created":{"date-parts":[[2003,2,26]],"date-time":"2003-02-26T17:47:22Z","timestamp":1046281642000},"page":"131-136","source":"Crossref","is-referenced-by-count":24,"title":["Immunoglobulins anti-<i>Anisakis simplex<\/i> in patients with gastrointestinal diseases"],"prefix":"10.1017","volume":"76","author":[{"given":"R.","family":"Guti\u00e9rrez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cu\u00e9llar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"S0022149X00701787_ref045","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/S0039-6109(16)42841-0","article-title":"Colonoscopic diagnosis of inflammatory bowel disease","volume":"62","author":"Wayne","year":"1982","journal-title":"Surgery North America"},{"key":"S0022149X00701787_ref039","first-page":"306","article-title":"Gastric anisakiasis diagnosed by endoscopy","volume":"20","author":"Romeo","year":"1997","journal-title":"Gastroenterology and Hepatology"},{"key":"S0022149X00701787_ref009","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1080\/00365549850161179","article-title":"Effects of Anisakis simplex on nitric oxide production in J774 macrophages","volume":"30","author":"Cu\u00e9llar","year":"1998","journal-title":"Scandinavian Journal of Infectious Diseases"},{"key":"S0022149X00701787_ref042","first-page":"101","volume-title":"Inflammatory bowel disease","author":"Thayer","year":"1987"},{"key":"S0022149X00701787_ref021","first-page":"8","article-title":"Acute regional ileitis due to Anisakis larvae","volume":"55","author":"Hayasaka","year":"1971","journal-title":"International Surgery"},{"key":"S0022149X00701787_ref017","first-page":"21","article-title":"Eosinophilic gastritis due to Anisakis: a case report","volume":"28","author":"Esteve","year":"2000","journal-title":"Allergologie et Immunopathologie (Madrid)"},{"key":"S0022149X00701787_ref016","unstructured":"Enna, (1995) Encuesta de Presupuestos Familiares 1990\u201391. Estudio Nacional de Nutrici\u00f3n y Alimentaci\u00f3n 1991. Madrid, INI."},{"key":"S0022149X00701787_ref027","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1746.2000.02153.x"},{"key":"S0022149X00701787_ref044","first-page":"75","article-title":"The present state of anisakiasis and its causative worms","volume":"28","author":"Van Thiel","year":"1976","journal-title":"Tropical and Geographical Medicine"},{"key":"S0022149X00701787_ref031","first-page":"70","article-title":"Gastrointestinal anisakiasis. Seven cases in three months","volume":"91","author":"Olveira","year":"1999","journal-title":"Revista Espa\u00f1ola de Enfermedades Digestivas"},{"key":"S0022149X00701787_ref029","first-page":"174","article-title":"Obstrucci\u00f3n intestinal por anisakiosis. Aportaci\u00f3n de dos nuevos casos","volume":"59","author":"Mart\u00edn","year":"1996","journal-title":"Cirug\u00eda Espa\u00f1ola"},{"key":"S0022149X00701787_ref008","first-page":"631","article-title":"Hallazgo endosc\u00f3pico de una larva de gusano en el fondo de \u00falcera g\u00e1strica","volume":"13","author":"Cruchaga","year":"1995","journal-title":"Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica"},{"key":"S0022149X00701787_ref003","first-page":"355","article-title":"Anisakiasis como causa de apendicits aguna y cuadro reumatol\u00f3gico: primer caso en la literatura m\u00e9dica","volume":"79","author":"Arenal","year":"1991","journal-title":"Revista Espa\u00f1ola de Enfermedades Digestivas"},{"key":"S0022149X00701787_ref011","first-page":"370","article-title":"Anisakiasis gastroal\u00e9rgica con parasitaci\u00f3n masiva","volume":"12","author":"Daschner","year":"1997","journal-title":"Revista Espa\u00f1ola de Alergolog\u00eda e Inmunolog\u00eda Cl\u00ednica"},{"key":"S0022149X00701787_ref025","first-page":"203","article-title":"Angiostrongylus costaricensis enterocolitis mimics Crohn's disease","volume":"16","author":"Liacouras","year":"1993","journal-title":"Journal of Pediatric Gastroenterology and Nutrition"},{"key":"S0022149X00701787_ref041","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)01000-1"},{"key":"S0022149X00701787_ref001","first-page":"59","article-title":"Dos casos de anisakiasis en Hospotal del R\u00edo Hortega (Valladolid).","volume":"88","author":"Acebes","year":"1996","journal-title":"Revista Espa\u00f1ola de Enfermedades Digestivas"},{"key":"S0022149X00701787_ref012","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00569.x"},{"key":"S0022149X00701787_ref010","first-page":"127","volume-title":"Inflammatory bowel disease","author":"Dalton","year":"1992"},{"key":"S0022149X00701787_ref037","first-page":"74","article-title":"Anisakiasis, una causa infrecuente de abdomen agudo","volume":"60","author":"Rodr\u00edguez","year":"1996","journal-title":"Cirug\u00eda Espa\u00f1ola"},{"key":"S0022149X00701787_ref006","first-page":"8","article-title":"Anisakiosis g\u00e1strica aguda por cocci\u00f3n insuficiente en horno microondas","volume":"106","author":"Canut","year":"1996","journal-title":"Medicina Cl\u00ednica"},{"key":"S0022149X00701787_ref023","first-page":"1270","volume-title":"Gastrointestinal disease","author":"Kornbluth","year":"1993"},{"key":"S0022149X00701787_ref007","first-page":"445","article-title":"A live Anisakis physeteris larva found in the abdominal cavity of a woman in Zaragoza, Spain","volume":"5","author":"Clavel","year":"1993","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00701787_ref019","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-200012030-00012"},{"key":"S0022149X00701787_ref028","first-page":"158","article-title":"Anisakidosis en Espa\u00f1a. Descripci\u00f3n de tres casos","volume":"10","author":"L\u00f3pez-V\u00e9lez","year":"1992","journal-title":"Enfermedades Infeccciosas y Microbiolog\u00eda Cl\u00ednica"},{"key":"S0022149X00701787_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(93)90087-7"},{"key":"S0022149X00701787_ref002","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00012359"},{"key":"S0022149X00701787_ref020","first-page":"214","article-title":"A simple index of Crohn's disease activity","volume":"347","author":"Harvey","year":"1996","journal-title":"Lancet"},{"key":"S0022149X00701787_ref036","doi-asserted-by":"publisher","DOI":"10.2214\/ajr.119.2.329"},{"key":"S0022149X00701787_ref024","doi-asserted-by":"publisher","DOI":"10.3109\/00365528909091339"},{"key":"S0022149X00701787_ref004","first-page":"723","article-title":"Human anisakiasis","volume":"2","author":"Bier","year":"1987","journal-title":"Bailiere's Clinical Tropical Medicine and Communicable Diseases"},{"key":"S0022149X00701787_ref035","first-page":"47","article-title":"Biological study of a case of colonic anisakiasis. Review of the literature","volume":"40","author":"Petithory","year":"1992","journal-title":"Pathology and Biology"},{"key":"S0022149X00701787_ref022","first-page":"43","volume-title":"Pogress in clinical parasitology. Vol. III","author":"Ishikura","year":"1993"},{"key":"S0022149X00701787_ref043","unstructured":"Valero, A. , Mira, J. , P\u00e9rez, R. , Sanz, J. , Gerald\u00eda, M. , Garc\u00eda, J. , Garc\u00eda, P. & Adroher, E. (1992) Descripci\u00f3n de un caso de anisakiosis humana. IX Reuni\u00f3n Cient\u00edfica de la Asociaci\u00f3n de Parasit\u00f3logos Espa\u00f1oles."},{"key":"S0022149X00701787_ref015","first-page":"132","article-title":"Anisakis simplex: a case of intestinal pseudo-obstruction","volume":"92","author":"Dom\u00ednguez","year":"2000","journal-title":"Revista Espa\u00f1ola de Enfermedades Digestivas"},{"key":"S0022149X00701787_ref040","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.3.278"},{"key":"S0022149X00701787_ref038","first-page":"693","article-title":"H\u00e9morragie digestive grave et recidivante: une forme exceptionelle de bilharziose colique","volume":"20","author":"Rolachon","year":"1996","journal-title":"Gastroenterologie et Cl\u00ednique Biologie"},{"key":"S0022149X00701787_ref030","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1066545"},{"key":"S0022149X00701787_ref005","first-page":"13","article-title":"First case of extra-gastrointestinal anisakiasis in a human diagnosed in Italy","volume":"39","author":"Cancrini","year":"1997","journal-title":"Parassitologia"},{"key":"S0022149X00701787_ref034","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(95)00111-5"},{"key":"S0022149X00701787_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7753(00)71235-8"},{"key":"S0022149X00701787_ref032","first-page":"301","volume-title":"Anisakis","author":"Oshima","year":"1972"},{"key":"S0022149X00701787_ref033","doi-asserted-by":"publisher","DOI":"10.2307\/3280642"},{"key":"S0022149X00701787_ref018","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X0001556X"},{"key":"S0022149X00701787_ref013","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.6.1392"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00701787","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T08:59:09Z","timestamp":1713171549000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00701787\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["S0022149X00701787"],"URL":"https:\/\/doi.org\/10.1079\/joh2001104","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]}}}