{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T13:18:15Z","timestamp":1758633495894},"reference-count":65,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"unspecified","delay-in-days":8078,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mixed-function oxidase enzymes metabolize most xenobiotic agents. Western blotting was used to investigate the effect of <jats:italic>Schistosoma mansoni<\/jats:italic> infection on the expression of various cytochrome P450 (CYP) isozymes and specific enzyme assays to study related metabolic functions in mouse liver microsomes. Male BK-TO mice were infected with 200 cercariae per mouse and their livers were assayed at 6, 15, 30 and 45 days post-infection (p.i.) and compared with appropriately matched controls. The expression of each of the CYP isozymes (1A1, 2B1\/2, 2C6, and 4A) was either unaffected or transiently increased up to 30 days post-infection. By 45 days, a significant loss of signal was observed, particularly for CYP 1A1 and 2B1\/2 where no signal could be detected. Evidence supporting these findings was obtained from enzyme assays specific for particular CYP isozymes. The activity of ethoxyresorufin <jats:italic>O<\/jats:italic>-deethylase (CYP 1A1) was reduced by 97% and that of pentoxyresorufin <jats:italic>O<\/jats:italic>-depentylase (CYP 2B1\/2) by 96% at 45 days p.i. Similarly, the activity of ethoxycoumarin hydroxylase was progressively reduced over the period under study. It is believed that <jats:italic>N<\/jats:italic>-nitrosamines are activated principally by <jats:italic>N<\/jats:italic>-nitrosodimethylamine <jats:italic>N<\/jats:italic>-demethylase I which was significantly increased at both 30 and 45 days p.i. To further investigate metabolic competency following <jats:italic>S. mansoni<\/jats:italic> infection, the <jats:italic>in vitro<\/jats:italic> binding of benzo(<jats:italic>a<\/jats:italic>)pyrene metabolites to DNA was measured, using isolated liver microsomes to activate benzo(<jats:italic>a<\/jats:italic>)pyrene. Benzo(<jats:italic>a<\/jats:italic>)pyrene-DNA adduct formation was markedly increased at 6, 15 and 30 days with a maximum at 15 days, but decreased at 45 days p.i. It was concluded that <jats:italic>S. mansoni<\/jats:italic> infection changes the expression of different CYP isozymes and also the activity of phase I drug-metabolizing enzymes at different periods of infection and may thus change the liver's capacity to activate or detoxify many endogenous and exogenous compounds. Such alterations may also change the therapeutic actions of drugs that are primarily metabolized by the P450 system, when administered to patients with schistosomiasis.<\/jats:p>","DOI":"10.1079\/joh200186","type":"journal-article","created":{"date-parts":[[2003,2,26]],"date-time":"2003-02-26T17:47:22Z","timestamp":1046281642000},"page":"71-78","source":"Crossref","is-referenced-by-count":18,"title":["Changes in the expression of cytochrome P450 isozymes and related carcinogen metabolizing enzyme activities in <i>Schistosoma mansoni<\/i>-infected mice"],"prefix":"10.1017","volume":"76","author":[{"given":"S.A.","family":"Sheweita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Mubark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.J.","family":"Doenhoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.H.","family":"Mostafa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.P.","family":"Margison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.J.","family":"O'Connor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.H.","family":"Elder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"S0022149X00701696_ref042","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(93)90018-5"},{"key":"S0022149X00701696_ref065","first-page":"463","article-title":"Biotransformation of coumarin by rodent and human cytochromes P-450: metabolic basis of tissue-selective toxicity in olfactory mucosa of rats and mice","volume":"288","author":"Zhuo","year":"1999","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"S0022149X00701696_ref003","doi-asserted-by":"publisher","DOI":"10.1007\/BF00308517"},{"key":"S0022149X00701696_ref041","first-page":"41","article-title":"Experimental study of the influence of Schistosoma japonicum infection on carcinogenesis of mouse liver treated with N-2-fluorenylacetamide (2-FAA)","volume":"33","author":"Miyasato","year":"1984","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00701696_ref026","first-page":"598","article-title":"The influence of bilharzial infection on the carcinogenesis of the bladder. An experimental study","volume":"44","author":"Hashem","year":"1961","journal-title":"Journal of the Egyptian Medical Association"},{"key":"S0022149X00701696_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(85)90355-7"},{"key":"S0022149X00701696_ref057","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.2570040203"},{"key":"S0022149X00701696_ref044","doi-asserted-by":"publisher","DOI":"10.1042\/bj0550416"},{"key":"S0022149X00701696_ref046","first-page":"3","volume-title":"N-nitroso compounds: occurrence, biological effects and relevance to human cancer","author":"Preussmann","year":"1984"},{"key":"S0022149X00701696_ref039","first-page":"1499","article-title":"Determinants of polycyclic aromatic hydrocarbons-DNA adducts in human placenta","volume":"52","author":"Manchester","year":"1992","journal-title":"Cancer Research"},{"key":"S0022149X00701696_ref006","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/5.11.1381"},{"key":"S0022149X00701696_ref031","first-page":"53","article-title":"Stereoselective metabolic activation of polycyclic aromatic hydrocarbons","volume":"9","author":"Jerina","year":"1979","journal-title":"Advanced Pharmacology and Therapeutics"},{"key":"S0022149X00701696_ref033","doi-asserted-by":"publisher","DOI":"10.3109\/01480549209014160"},{"key":"S0022149X00701696_ref048","doi-asserted-by":"publisher","DOI":"10.2174\/1389200003339117"},{"key":"S0022149X00701696_ref055","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(82)90306-4"},{"key":"S0022149X00701696_ref015","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(87)90541-7"},{"key":"S0022149X00701696_ref016","doi-asserted-by":"publisher","DOI":"10.1126\/science.3941892"},{"key":"S0022149X00701696_ref017","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1980.60.4.1107"},{"key":"S0022149X00701696_ref028","first-page":"90","volume-title":"Nitrosamine toxicology and microbiology","author":"Hill","year":"1988"},{"key":"S0022149X00701696_ref034","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(99)00010-1"},{"key":"S0022149X00701696_ref054","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000086248"},{"key":"S0022149X00701696_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(96)03366-5"},{"key":"S0022149X00701696_ref060","volume-title":"The control of schistosomiasis. Report of WHO Expert Committee","year":"1985"},{"key":"S0022149X00701696_ref050","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(96)04322-4"},{"key":"S0022149X00701696_ref063","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/11.12.2239"},{"key":"S0022149X00701696_ref011","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/61.1.13"},{"key":"S0022149X00701696_ref022","first-page":"769","article-title":"Influence of Schistosoma mansoni infection on carcinogen-metaboliz-ing capacities and in vitro aflatoxin Ba metabolism in human liver.","volume":"3","author":"Habib","year":"1996","journal-title":"Oncology Reports"},{"key":"S0022149X00701696_ref025","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6672"},{"key":"S0022149X00701696_ref032","doi-asserted-by":"publisher","DOI":"10.2739\/kurumemedj.32.169"},{"key":"S0022149X00701696_ref062","doi-asserted-by":"publisher","DOI":"10.3109\/03602539009041082"},{"key":"S0022149X00701696_ref061","volume-title":"The epidemiological association between Schistosoma haematobium infection and bladder cancer","year":"1986"},{"key":"S0022149X00701696_ref020","first-page":"596","article-title":"An improved assay of 7-ethoxycoumarin O-deethylase activity: induction of hepatic enzyme activity in C57BL\/6J and DBA\/2J mice by phenobarbital, 3-methylcholantherene and 2,3,7,8-tetrachlorodibenzo-p-dioxin","volume":"205","author":"Greenle","year":"1978","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"S0022149X00701696_ref064","first-page":"1499","article-title":"Metabolism of N-nitrosodialkylamines by human liver microsomes","volume":"88","author":"Yoo","year":"1988","journal-title":"Cancer Research"},{"key":"S0022149X00701696_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6535(96)00169-5"},{"key":"S0022149X00701696_ref023","first-page":"3578","article-title":"Metabolic oxidation of carcinogenic arylamines by rat, dog and human hepatic microsomes and by purified flavin-containing and cytochrome P450 monooxygenases","volume":"45","author":"Hammons","year":"1985","journal-title":"Cancer Research"},{"key":"S0022149X00701696_ref049","first-page":"155","article-title":"Recovery of the hepatic carcinogen-metabolizing capacity in schistosome-infected mice after treatment with the antischistosomal praziquantel","volume":"2","author":"Sheweita","year":"1995","journal-title":"Oncology Reports"},{"key":"S0022149X00701696_ref047","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/12.3.503"},{"key":"S0022149X00701696_ref013","first-page":"65","article-title":"DNA methylation in individuals at high risk for stomach cancer","volume":"13","author":"Cooper","year":"1991","journal-title":"British Journal of Cancer"},{"key":"S0022149X00701696_ref059","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/8.12.1971"},{"key":"S0022149X00701696_ref040","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(74)90291-3"},{"key":"S0022149X00701696_ref004","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910280319"},{"key":"S0022149X00701696_ref002","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1987.tb04580.x"},{"key":"S0022149X00701696_ref018","doi-asserted-by":"publisher","DOI":"10.3109\/03602539409029789"},{"key":"S0022149X00701696_ref014","first-page":"213","article-title":"Carcinoma of the bladder in urinary schistosomiasis","volume":"20","author":"Delmas","year":"1986","journal-title":"Annals of Urology"},{"key":"S0022149X00701696_ref052","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00001012"},{"key":"S0022149X00701696_ref045","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90505-9"},{"key":"S0022149X00701696_ref043","first-page":"15","article-title":"Modification of the oxidative N-demethylation of dimethylnitrosamine by various anti-inflammatory drugs","volume":"2","author":"Mostafa","year":"1992","journal-title":"Ramazzini Newsletters"},{"key":"S0022149X00701696_ref019","first-page":"1569","article-title":"Depression of the hepatic cytochrome P450 monooxygenase system by treatment of mice with the anti-neoplastic agent, 5-azacytidine","volume":"45","author":"Gooderham","year":"1985","journal-title":"Cancer Research"},{"key":"S0022149X00701696_ref012","first-page":"4875","article-title":"Induction of microsomal enzymes by foreign chemicals and carcinogenesis by polycyclic aromatic hydrocarbons","volume":"42","author":"Conney","year":"1982","journal-title":"Cancer Research"},{"key":"S0022149X00701696_ref038","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"S0022149X00701696_ref053","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(90)90703-N"},{"key":"S0022149X00701696_ref037","doi-asserted-by":"publisher","DOI":"10.1038\/239165b0"},{"key":"S0022149X00701696_ref030","doi-asserted-by":"publisher","DOI":"10.3109\/00498259009046845"},{"key":"S0022149X00701696_ref036","doi-asserted-by":"publisher","DOI":"10.3109\/00498259609046709"},{"key":"S0022149X00701696_ref027","first-page":"421","article-title":"The effect of schistosomiasis on the activation of aflatoxin B1","volume":"51","author":"Hasler","year":"1986","journal-title":"Research Communications in Pathology and Pharmacology"},{"key":"S0022149X00701696_ref029","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(94)90247-X"},{"key":"S0022149X00701696_ref005","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/14.4.653"},{"key":"S0022149X00701696_ref021","doi-asserted-by":"publisher","DOI":"10.1021\/tx00022a001"},{"key":"S0022149X00701696_ref009","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.20.7696"},{"key":"S0022149X00701696_ref058","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(68)90217-8"},{"key":"S0022149X00701696_ref035","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(89)90336-0"},{"key":"S0022149X00701696_ref056","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910360607"},{"key":"S0022149X00701696_ref007","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.1993.tb01359.x"},{"key":"S0022149X00701696_ref051","first-page":"119","article-title":"Schistosomiasis induced changes in glutathione levels and glutathione reductase\/glutathione S-transferase activities in human liver","volume":"56","author":"Sheweita","year":"1997","journal-title":"Biomedical Letters"},{"key":"S0022149X00701696_ref010","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940070410"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00701696","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T02:22:28Z","timestamp":1712888548000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00701696\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3]]},"references-count":65,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,3]]}},"alternative-id":["S0022149X00701696"],"URL":"https:\/\/doi.org\/10.1079\/joh200186","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,3]]}}}