{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T16:52:18Z","timestamp":1761843138330},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"unspecified","delay-in-days":7621,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mucosal mast cells (MMC) play an important role in the immune response against selected species of intestinal nematode. The kinetics with which different strains of inbred mice resolve infection with <jats:italic>Trichinella spiralis<\/jats:italic> correlates with their ability to mount MMC responses in the intestinal mucosa. Homologues of MMC that express and constitutively secrete abundant amounts of the granule chymase, mouse mast cell protease-1 (mMCP-1), can be generated <jats:italic>in vitro<\/jats:italic> from bone marrow cultures supplemented with interleukins-3 and -9, stem cell factor and transforming growth factor-\u03b2<jats:sub>1<\/jats:sub>. Using the enhanced growth characteristics of these MMC homologues, a novel limiting dilution assay for mast cell precursor (MCp) frequency has been developed. The assay is highly specific, in that cultures containing mast cells are identified with mMCP-1 specific antibody, and almost three-fold more sensitive than previously published systems. MCp frequencies were compared in BALB\/c and C57\/BL10 strains of mice that, respectively, respond rapidly and slowly to infection with <jats:italic>T. spiralis<\/jats:italic>. MCp frequency (1\/378 bone marrow cells) was significantly greater (P&lt;0.05) in BALB\/c than C57\/BL10 mice (frequency: 1\/751). Similarly the rate of growth of MMC homologues and the production of mMCP-1 was significantly (P&lt;0.05) greater in BALB\/c than in C57\/BL10 bone marrow cultures.<\/jats:p>","DOI":"10.1079\/joh2002160","type":"journal-article","created":{"date-parts":[[2003,5,22]],"date-time":"2003-05-22T21:50:12Z","timestamp":1053640212000},"page":"155-161","source":"Crossref","is-referenced-by-count":9,"title":["Mucosal mast cells and nematode infection: strain-specific differences in mast cell precursor frequency revisited"],"prefix":"10.1017","volume":"77","author":[{"given":"J.K.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.H.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.R.P.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"S0022149X00702380_ref028","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2002.01233.x"},{"key":"S0022149X00702380_ref015","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.10.3473.410k01_3473_3486"},{"key":"S0022149X00702380_ref006","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1993.tb00572.x"},{"key":"S0022149X00702380_ref020","first-page":"1661","article-title":"Mast cell heterogeneity in the gastrointestinal tract: variable expression of mouse mast cell protease-1 (mMCP-1) in intraepithelial mucosal mast cells in nematode-infected and normal BALB\/c mice","volume":"150","author":"Scudamore","year":"1997","journal-title":"American Journal of Pathology"},{"key":"S0022149X00702380_ref024","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(86)90373-1"},{"key":"S0022149X00702380_ref009","doi-asserted-by":"publisher","DOI":"10.1084\/jem.150.3.482"},{"key":"S0022149X00702380_ref014","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(96)05696-6"},{"key":"S0022149X00702380_ref005","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1056\/NEJM199301283280408","article-title":"New concepts about the mast cell","volume":"328","author":"Galli","year":"1993","journal-title":"New England Journal of Medicine"},{"key":"S0022149X00702380_ref027","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00155.x"},{"key":"S0022149X00702380_ref001","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200006)30:6<1656::AID-IMMU1656>3.0.CO;2-Z"},{"key":"S0022149X00702380_ref004","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/8.4.559"},{"key":"S0022149X00702380_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80545-0"},{"key":"S0022149X00702380_ref021","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182096008384"},{"key":"S0022149X00702380_ref025","first-page":"787","article-title":"Genetic control of immunity to Trichinella spiralis. Donor bone marrow cells determine responses to infection in mouse radiation chimaeras","volume":"43","author":"Wakelin","year":"1981","journal-title":"Immunology"},{"key":"S0022149X00702380_ref019","first-page":"510","article-title":"Genetic control of mast cell development in bone marrow cultures","volume":"73","author":"Reed","year":"1988","journal-title":"Strain-dependent variation in cultures from inbred mice. Clinical and Experimental Immunology"},{"key":"S0022149X00702380_ref026","first-page":"103","article-title":"T and B cells in the transfer of immunity against Trichinella spiralis in mice","volume":"37","author":"Wakelin","year":"1979","journal-title":"Immunology"},{"key":"S0022149X00702380_ref003","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1986.tb00865.x"},{"key":"S0022149X00702380_ref023","doi-asserted-by":"publisher","DOI":"10.1038\/273617a0"},{"key":"S0022149X00702380_ref007","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.9.1243"},{"key":"S0022149X00702380_ref002","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.146.1.211"},{"key":"S0022149X00702380_ref010","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64236-8"},{"key":"S0022149X00702380_ref008","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.5.1334.bloodjournal8551334"},{"key":"S0022149X00702380_ref012","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.1.355"},{"key":"S0022149X00702380_ref011","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.12.1849"},{"key":"S0022149X00702380_ref022","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1990.tb00996.x"},{"key":"S0022149X00702380_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(79)90188-6"},{"key":"S0022149X00702380_ref013","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1997.77.4.1033"},{"key":"S0022149X00702380_ref018","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(78)90172-7"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00702380","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T02:21:15Z","timestamp":1712888475000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00702380\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["S0022149X00702380"],"URL":"https:\/\/doi.org\/10.1079\/joh2002160","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,6]]}}}