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Helminthol."],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Adult <jats:italic>Ascaris suum<\/jats:italic> were dissected to obtain different worm components (body wall, body fluid, ovaries, uterus and oesophagus) which were used as antigens when testing 95 sera of naturally <jats:italic>A. suum<\/jats:italic>-infected Iberian pigs by enzyme-linked immunosorbent assay (ELISA) and Western blot (WB). Pigs with patent <jats:italic>Ascaris<\/jats:italic> infections had significantly lower ELISA optical density values than pigs without adult worms when using the body fluid and the body wall as antigens. A poor negative correlation was found between adult intestinal worm burden or eggs in faeces and specific antibody responses, measured by ELISA and WB using all antigens. By WB, the recognition of specific bands was variable, but three groups of bands with molecular weights of 97 kDa, 54\u201358 kDa and 42\u201344 kDa were generally recognized by sera from naturally infected pigs as well as from hyperimmunized pigs when using the five antigen extracts. The ELISA and WB techniques may be used for immunodiagnosis, using somatic adult worm antigens, to declare young pigs to be <jats:italic>Ascaris<\/jats:italic>-free but cannot be used for individual <jats:italic>Ascaris<\/jats:italic>-diagnosis in adult Iberian pigs.<\/jats:p>","DOI":"10.1079\/joh2002163","type":"journal-article","created":{"date-parts":[[2003,5,22]],"date-time":"2003-05-22T21:50:12Z","timestamp":1053640212000},"page":"167-172","source":"Crossref","is-referenced-by-count":9,"title":["Serological responses to <i>Ascaris suum<\/i> adult worm antigens in Iberian finisher pigs"],"prefix":"10.1017","volume":"77","author":[{"given":"E.","family":"Frontera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Serrano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Reina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Alcaide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"S\u00e1nchez-L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Navarrete","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"S0022149X00702409_ref015","doi-asserted-by":"publisher","DOI":"10.1007\/BF00931899"},{"key":"S0022149X00702409_ref025","first-page":"152","article-title":"Unabbreviated Ascaris suum immunising infections of piglets lead to immunosupression and increased numbers of intestinal parasites","volume":"40","author":"Stankiewicz","year":"1995","journal-title":"Acta Parasitologica"},{"key":"S0022149X00702409_ref026","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(92)90007-8"},{"key":"S0022149X00702409_ref005","first-page":"221","article-title":"Investigations into the course of experimental Ascaris suum invasions in pigs under field conditions","volume":"21","author":"Buchwalder","year":"1984","journal-title":"Helminthology"},{"key":"S0022149X00702409_ref013","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(79)90069-9"},{"key":"S0022149X00702409_ref028","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X0003368X"},{"key":"S0022149X00702409_ref012","doi-asserted-by":"publisher","DOI":"10.1017\/S003118209700142X"},{"key":"S0022149X00702409_ref010","first-page":"191","article-title":"Diagnosis of hepatic milk spots in swine using enzyme-linked immunosorbent assay with adult female Ascaris suum antigen","volume":"48","author":"Furuya","year":"1995","journal-title":"Journal of Veterinary Medicine"},{"key":"S0022149X00702409_ref022","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182097001480"},{"key":"S0022149X00702409_ref017","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(77)90093-5"},{"key":"S0022149X00702409_ref014","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"S0022149X00702409_ref021","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(97)00022-2"},{"key":"S0022149X00702409_ref020","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050444"},{"key":"S0022149X00702409_ref029","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0720(15)31218-4"},{"key":"S0022149X00702409_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(97)00054-4"},{"key":"S0022149X00702409_ref007","unstructured":"Eriksen, L. 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