{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T03:10:48Z","timestamp":1712891448856},"reference-count":34,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"unspecified","delay-in-days":7072,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 175 kDa antigen fraction with collagenase activity was isolated and purified from somatic extracts of adult <jats:italic>Setaria cervi<\/jats:italic> females using column chromatography involving consecutive steps of DEAE-Sepharose CL6B and Sephadex G-100. The optimum pH for 175 kDa collagenase was found to be pH 7.0. Sensitivities to a variety of inhibitors and activators indicated that the 175 kDa coIlagenolytic enzyme was metalloserine in nature. The enzyme hydrolysed a variety of protein substrates such as haemoglobin, casein, azocasein (general substrates) and collagen, FALGPA (furanoyl-acryloyl-leu-gly-pro-ala), the specific substrate of collagenase. The enzyme showed 57% inhibition by jird anti-somatic collagenase antibodies and reacted insignificantly with normal jird sera. Further analysis was undertaken on the immunoprophylactic potential of 175 kDa collagenase in inducing immunity against <jats:italic>Brugia malayi<\/jats:italic> (a human filarial parasite) in jirds (<jats:italic>Meriones unguiculatus<\/jats:italic>) <jats:italic>in vitro<\/jats:italic> and <jats:italic>in situ<\/jats:italic>. Immune sera of jirds raised against this antigen promoted partial adherence of peritoneal exudate cells to <jats:italic>B. malayi<\/jats:italic> microfilariae (mf) and infective larvae (L3) <jats:italic>in vitro<\/jats:italic> and induced partial cytotoxicity to the parasites within 48 h. The anti-<jats:italic>S. cervi<\/jats:italic> 175 kDa antigen serum was more effective in inducing cytotoxicity to <jats:italic>B. malayi<\/jats:italic> L3, than mf. In the microchambers implanted inside immune jirds, host cells could migrate and adhere to the mf and infective larvae thereby killing them partially within 48 h.<\/jats:p>","DOI":"10.1079\/joh2004254","type":"journal-article","created":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T23:41:04Z","timestamp":1101944464000},"page":"347-352","source":"Crossref","is-referenced-by-count":7,"title":["Adult 175 kDa collagenase antigen of <i>Setaria cervi<\/i> in immunoprophylaxis against <i>Brugia malayi<\/i> in jirds"],"prefix":"10.1017","volume":"78","author":[{"given":"Y.","family":"Srivastava","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Rathaur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.P.","family":"Bhandari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.V.R.","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.C.","family":"Harinath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"S0022149X00703269_ref017","doi-asserted-by":"publisher","DOI":"10.1053\/rvsc.2000.0425"},{"key":"S0022149X00703269_ref011","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.127.2.716"},{"key":"S0022149X00703269_ref006","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(90)90005-8"},{"key":"S0022149X00703269_ref031","volume-title":"Fifth report on filariasis","year":"1987"},{"key":"S0022149X00703269_ref007","doi-asserted-by":"publisher","DOI":"10.1007\/BF02703286"},{"key":"S0022149X00703269_ref009","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)45284-2"},{"key":"S0022149X00703269_ref012","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39614-X"},{"key":"S0022149X00703269_ref032","first-page":"73","article-title":"Studies on Dipetalonema viteae (Filarioidea) III. 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