{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:11:11Z","timestamp":1789873871451,"version":"4.0.1"},"reference-count":25,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"unspecified","delay-in-days":7072,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A dot-blot enzyme-linked immunosorbent assay (dot-blot ELISA) using an electroeluted 31-kDa glycoprotein from adult worms of <jats:italic>Parastrongylus cantonensis<\/jats:italic> as the specific antigen was evaluated for the immunological diagnosis of patients infected with <jats:italic>P. cantonensis<\/jats:italic>. The sensitivity and specificity for the detection of serum antibody to <jats:italic>P. cantonensis<\/jats:italic> in dot-blot ELISA were both 100%, as determined with serum samples of ten <jats:italic>P. cantonensis<\/jats:italic>-infected patients, 60 patients with other related parasitic infections, and 20 uninfected controls. The test was as sensitive and specific as the immunoblot test which revealed a reactive band of 31 kDa. Both the dot-blot ELISA and immunoblot detected all sera from ten <jats:italic>P. cantonensis<\/jats:italic>-infected individuals, but not with those of other heterologous parasitoses (gnathostomiasis, toxocariasis, filariasis, paragonimiasis, cysticercosis and malaria) or sera from healthy controls. The dot-blot ELISA is much simpler to perform than the immunoblot technique, and the test can be applied under field conditions where sophisticated facilities are lacking.<\/jats:p>","DOI":"10.1079\/joh2004257","type":"journal-article","created":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T23:41:04Z","timestamp":1101944464000},"page":"287-291","source":"Crossref","is-referenced-by-count":16,"title":["A dot-blot ELISA comparable to immunoblot for the specific diagnosis of human 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