{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T14:11:55Z","timestamp":1771683115280,"version":"3.50.1"},"reference-count":16,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"unspecified","delay-in-days":6707,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It is now recognized that the morphospecies <jats:italic>Anisakis simplex<\/jats:italic> is not a single species but a complex composed of three sibling species, <jats:italic>A. simplex<\/jats:italic> sensu stricto, <jats:italic>A. pegreffii<\/jats:italic> and <jats:italic>A. simplex<\/jats:italic> C. In Japan, <jats:italic>A. simplex<\/jats:italic>-like larvae have been isolated from a variety of fish and humans, but the larvae collected have been identified as <jats:italic>A. simplex<\/jats:italic> by only light microscopy. Therefore, the epidemiology of the <jats:italic>A. simplex<\/jats:italic> complex, composed of three sibling species, is still unclear in Japan. In the present study, 26 <jats:italic>A. simplex<\/jats:italic>-like larval isolates were obtained from two Pacific cod landed in Hokkaido, Japan, and examined genetically by PCR\u2013RFLP and direct sequencing of the ITS region of rDNA. Among the 26 isolates, 24 were identified as <jats:italic>A. simplex<\/jats:italic> sensu stricto, the other two as <jats:italic>A. pegreffii<\/jats:italic>. The present study is the first to confirm the distribution of <jats:italic>A. pegreffii<\/jats:italic> in Japan, and to detect <jats:italic>A. pegreffii<\/jats:italic> larvae in Pacific cod.<\/jats:p>","DOI":"10.1079\/joh2005290","type":"journal-article","created":{"date-parts":[[2005,12,3]],"date-time":"2005-12-03T00:36:27Z","timestamp":1133570187000},"page":"303-306","source":"Crossref","is-referenced-by-count":46,"title":["Molecular characterization of <i>Anisakis pegreffii<\/i> larvae in Pacific cod in Japan"],"prefix":"10.1017","volume":"79","author":[{"given":"N.","family":"Abe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Ohya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Yanagiguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"S0022149X00703701_ref015","doi-asserted-by":"publisher","DOI":"10.12935\/jvma1951.51.525"},{"key":"S0022149X00703701_ref014","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.3.278"},{"key":"S0022149X00703701_ref012","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-68281-3_8"},{"key":"S0022149X00703701_ref002","first-page":"591","article-title":"Diagnosis of a case of gastric anisakidosis by PCR-based restriction fragment length polymorphism analysis","volume":"41","author":"D'Amelio","year":"1999","journal-title":"Parassitologia"},{"key":"S0022149X00703701_ref006","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000051544"},{"key":"S0022149X00703701_ref008","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(86)90032-9"},{"key":"S0022149X00703701_ref011","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/0020-7519(87)90117-2","article-title":"Effects of marine mammal parasites on human health","volume":"17","author":"Oshima","year":"1987","journal-title":"International Journal for Parasitology"},{"key":"S0022149X00703701_ref003","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00178-2"},{"key":"S0022149X00703701_ref009","first-page":"114","article-title":"Foodborne helminthiases as emerging diseases in Japan","volume":"25","year":"2004","journal-title":"Infectious Agents Surveillance Report"},{"key":"S0022149X00703701_ref001","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1348(03)00073-X"},{"key":"S0022149X00703701_ref004","first-page":"425","article-title":"Parasitological and epidemiological survey of anisakid larvae from sardines, Engraulis japonica, caught in the sea near Kamogawa City, Chiba Prefecture, Japan, where human anisakiasis prevailed","volume":"41","author":"Kato","year":"1992","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00703701_ref005","first-page":"466","article-title":"Morphological and taxonomical studies on Anisakidae larvae found in marine fishes and squids","volume":"18","author":"Koyama","year":"1969","journal-title":"Japanese Journal of Parasitology"},{"key":"S0022149X00703701_ref007","doi-asserted-by":"publisher","DOI":"10.2307\/3284402"},{"key":"S0022149X00703701_ref016","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(98)00150-7"},{"key":"S0022149X00703701_ref013","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006093201920"},{"key":"S0022149X00703701_ref010","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00008580"}],"container-title":["Journal of Helminthology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0022149X00703701","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T02:26:46Z","timestamp":1712888806000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0022149X00703701\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,12]]}},"alternative-id":["S0022149X00703701"],"URL":"https:\/\/doi.org\/10.1079\/joh2005290","relation":{},"ISSN":["0022-149X","1475-2697"],"issn-type":[{"value":"0022-149X","type":"print"},{"value":"1475-2697","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12]]}}}