{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:26:37Z","timestamp":1787826397176,"version":"build-2784847793"},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T00:00:00Z","timestamp":1712880000000},"content-version":"unspecified","delay-in-days":6798,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Helminthol."],"published-print":{"date-parts":[[2005,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Fasciola hepatica<\/jats:italic> has recently emerged as a major pathogen of humans from reports on areas of endemicity and hyper-endemicity for fascioliasis. This situation is aggravated by the lack of standard assays for the screen diagnosis of <jats:italic>F. hepatica<\/jats:italic> infection in humans living in endemic areas. Our laboratory has developed an enzyme-linked immunosorbent assay (Fas2-ELISA) based on the capture of IgG antibody by a purified protein Fas2, which is an adult fluke cysteine proteinase. Fas2-ELISA exhibited 95% sensitivity and 100% specificity in 38 individuals infected with <jats:italic>F. hepatica<\/jats:italic> diagnosed by finding eggs in stools and 46 serum samples from healthy volunteers. No cross-reaction was observed with 54 serum samples from patients with ten different parasitic infections including the trematodes <jats:italic>Paragonimus westermani<\/jats:italic> and <jats:italic>Schistosoma mansoni<\/jats:italic>. The high antigenicity of Fas2 is suggested by the fact that antibodies to Fas2 rise rapidly by 1\u20132 weeks of infection and rise until patency at 8 weeks of infection in experimentally infected alpacas. Field screening for human fascioliasis using Fas2-ELISA and coprology in three endemic locations of the Peruvian Andes resulted in 95.5% sensitivity, 86.6% specificity in a population of 664 children in an age range of 1 to 16 years old. These results provide evidence of the clinical potential of Fas2-ELISA to diagnose fascioliasis in humans exposed to liver fluke infection in endemic areas for this parasite. Fas2-ELISA is currently developed as a standard assay for both field screening for fascioliasis in people living in endemic areas and detecting occasionally <jats:italic>F. hepatica<\/jats:italic> infected patients in clinical laboratories.<\/jats:p>","DOI":"10.1079\/joh2005303","type":"journal-article","created":{"date-parts":[[2005,9,12]],"date-time":"2005-09-12T22:05:11Z","timestamp":1126562711000},"page":"235-240","source":"Crossref","is-referenced-by-count":39,"title":["Fas2-ELISA in the detection of human infection by <i>Fasciola 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