{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T07:58:10Z","timestamp":1759564690146},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,12,31]],"date-time":"2010-12-31T00:00:00Z","timestamp":1293753600000},"content-version":"vor","delay-in-days":1248,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Background.<\/jats:italic> Nestin is a type VI intermediate filament protein originally described in neural stem cells. Recent reports have documented nestin expression in endothelium of newly formed blood vessels and suggested its role as a marker of capacity for neovascularization and angiogenesis in endothelial cells. The aim of this study was to investigate the differential expression of nestin in normal and pre\u2010eclamptic human placentas. <jats:italic>Methods.<\/jats:italic> Placental tissues from 12 women with severe pre\u2010eclampsia and 15 gestational age\u2010matched normotensive women were collected at the time of their cesarean section. Western blot analysis for each placental tissue was performed for nestin quantification. Immunohistochemical staining was employed to localize nestin\u2010positive cells and to investigate differential staining intensity in each placental cell. <jats:italic>Results.<\/jats:italic> Nestin expression was detected in all of the normal and pre\u2010eclamptic placental tissues by Western blotting. Compared with the normal placentas, tissues from severe pre\u2010eclamptic placentas showed higher expression of nestin (<jats:italic>p<\/jats:italic>&lt;0.001). Nestin immunoreactivity was localized only to endothelial cells of chorionic villi. However, mesenchymal connective tissue cells, cytotrophoblasts, syncytiotrophoblasts, and decidual cells did not reveal any specific signal for nestin. <jats:italic>Conclusions.<\/jats:italic> We suggest that the capacity for neovascularization and angiogenesis in endothelial cell is increased in pre\u2010eclamptic placenta compared to that from normal pregnancy. Such changes may be a compensatory mechanism for the reduced maternofetal exchanges and long\u2010lasting fetal hypoxia in pre\u2010eclamptic pregnancy. Furthermore, these changes in endothelial cells of chorionic villi in pre\u2010eclamptic pregnancy may give an explanation for fetal response to pre\u2010eclamptic conditions.<\/jats:p>","DOI":"10.1080\/00016340701417018","type":"journal-article","created":{"date-parts":[[2007,7,25]],"date-time":"2007-07-25T03:45:44Z","timestamp":1185335144000},"page":"909-914","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Differential expression of nestin in normal and pre\u2010eclamptic human 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