{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T06:10:28Z","timestamp":1697091028071},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,12,31]],"date-time":"2010-12-31T00:00:00Z","timestamp":1293753600000},"content-version":"vor","delay-in-days":1248,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Background.<\/jats:italic> \u03b1\u2010Methyldopa is a classic antihypertensive agent, used routinely in the treatment of pregnancy\u2010induced hypertension. However, only a few data are available about its direct uterotropic effect. Accordingly, the aim of the present study was to investigate the direct effects of \u03b1\u2010methyldopa on the myometrial adrenergic functions in rat. <jats:italic>Methods.<\/jats:italic> The effects of \u03b1\u2010methyldopa on the sympathetic transmission in the non\u2010pregnant, early pregnant and late\u2010pregnant myometrium were investigated by a superfusion technique. Myometrial samples from control and \u03b1\u2010methyldopa\u2010treated (200 mg\/kg i.p. for 7 days) non\u2010pregnant, 7\u2010day and 21\u2010day pregnant rats were saturated with [<jats:sup>3<\/jats:sup>H]noradrenaline, and the liberation evoked by electric field stimulation was determined. The contractility responses to \u03b1\u2010 and \u03b2\u2010adrenergic stimulation were additionally characterised by generating concentration\u2013response curves of myometrial rings to noradrenaline and terbutaline in the same arrangement. The changes in the density and affinity of the adrenergic receptors (\u03b1<jats:sub>2<\/jats:sub> and \u03b2<jats:sub>2<\/jats:sub>) were investigated by a radioligand binding technique. <jats:italic>Results.<\/jats:italic> The treatment with \u03b1\u2010methyldopa substantially decreased both the [<jats:sup>3<\/jats:sup>H]noradrenaline uptake and release in both the non\u2010pregnant and early pregnant uterus, while treatment\u2010dependent changes were observed at term only in the uptake capacity. The contractility response to exogenous \u03b1\u2010sympathomimetics was higher in the group treated in early pregnancy, and a decreased terbutaline\u2010induced relaxation was observed in the non\u2010pregnant state and at term. The treatment resulted in increased affinity for \u03b1<jats:sub>2<\/jats:sub> receptors in early pregnancy, while K<jats:sub>d<\/jats:sub> for \u03b2<jats:sub>2<\/jats:sub> was increased at term. <jats:italic>Conclusions.<\/jats:italic> Our experimental data suggest that besides its antihypertensive effect, \u03b1\u2010methyldopa may influence the adrenergic transmission of the pregnant uterus. Our results indicate that the agent decreases the efficacy of \u03b2<jats:sub>2<\/jats:sub>\u2010adrenergic agonists at term pregnancy and increases the response to \u03b1\u2010sympathomimetics in early pregnancy.<\/jats:p>","DOI":"10.1080\/00016340701463830","type":"journal-article","created":{"date-parts":[[2007,7,25]],"date-time":"2007-07-25T03:45:51Z","timestamp":1185335151000},"page":"986-994","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The effects of \u03b1\u2010methyldopa on myometrial noradrenaline release and myometrial contractility in rat"],"prefix":"10.1111","volume":"86","author":[{"given":"D\u00c9NES","family":"CSONKA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ISTV\u00c1N","family":"ZUPK\u00d3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"REN\u00c1TA","family":"MINORICS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1RP\u00c1D","family":"M\u00c1RKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00c1BOR","family":"CS\u00cdK","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GEORGE","family":"FALKAY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,12,31]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.crd.0000137738.16166.cc"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/beog.2001.0232"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199607253350407"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-200209000-00016"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004040100214"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1973.tb08319.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1971.tb08671.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(73)90126-X"},{"key":"e_1_2_6_10_2","first-page":"552","article-title":"Differential competition by l\u2010alpha\u2010methyldopa metabolites for adrenergic receptors in rat forebrain","volume":"220","author":"Goldberg MR","year":"1982","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/S0002-9378(16)34239-9","article-title":"Autonomic innervation of the rat vagina, cervix, and uterus and its cyclic variation","volume":"104","author":"Adham N","year":"1969","journal-title":"Am J Obstet Gynecol"},{"key":"e_1_2_6_12_2","first-page":"657","article-title":"Autonomic nervous control of reproduction: circulatory and other factors","volume":"24","author":"Bell C","year":"1972","journal-title":"Pharmacol Rev"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00225412"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(79)90348-X"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00811"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(67)90297-7"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.131.3417.1890"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.30.1"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(84)80033-9"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00177750"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0530051"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(79)90224-0"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02691.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.3109\/10799899409066030"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1986.tb10197.x"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)90531-1"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)91564-H"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)91289-7"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/286159a0"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02190-4"}],"container-title":["Acta Obstetricia et Gynecologica Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1080%2F00016340701463830","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1080\/00016340701463830","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T01:59:21Z","timestamp":1697075961000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1080\/00016340701463830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1080\/00016340701463830"],"URL":"https:\/\/doi.org\/10.1080\/00016340701463830","archive":["Portico"],"relation":{},"ISSN":["0001-6349","1600-0412"],"issn-type":[{"value":"0001-6349","type":"print"},{"value":"1600-0412","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8]]},"assertion":[{"value":"2010-12-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}