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FAH were identified by immunohistochemical demonstration of glutathione- S-transferase (placental form, GSTP) and by staining with hematoxilin\/eosin (H&amp;E), and their area fraction was quantified morphometrically. The four model hepatocarcinogens N-nitrosomorpholine, 2-acetylaminofluoren, phenobarbital, and clofibrate were selected according to characteristic differences in their presumed mode of action, and tested in a total of 1,600 male and female rats at 2 different dose levels. The chemicals were found to differ characteristically in their potency and dose-response relationship to induce FAH when given alone or when administered following initiation with diethylnitrosamine. The interlaboratory variation was small for results obtained with the GSTP-stain and somewhat larger with respect to H&amp;E. The assessment of the carcinogenic potential of the four chemicals by the different laboratories was in the same range and the nature of their dose-response relationships did not differ essentially between laboratories. Our results suggest that this RLFB is a sensitive bioassay, providing potentially valuable information for risk assessment including the classification of carcinogenic chemicals according to their mode of action.<\/jats:p>","DOI":"10.1080\/01926230390173888","type":"journal-article","created":{"date-parts":[[2008,2,13]],"date-time":"2008-02-13T19:09:13Z","timestamp":1202929753000},"page":"60-79","source":"Crossref","is-referenced-by-count":5,"title":["Prevalidation of a Rat Liver Foci Bioassay (RLFB) Based on Results from 1600 Rats: A Study Report"],"prefix":"10.1177","volume":"31","author":[{"given":"Carina","family":"Ittrich","sequence":"first","affiliation":[{"name":"Central Unit Biostatistics German Cancer research Center (DKFZ), P.O. 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