{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:39:48Z","timestamp":1778780388936,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicol Pathol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p>Different degrees of a toxic response between and within the various lobes of the liver have been observed in rodents following treatment with acetaminophen. This study was designed to compare 2 sampling methods of the rat liver for gene-expression analysis. Ten male Fischer 344\/N rats, 12\u201314 weeks of age, were treated with vehicle (0.5% aqueous ethyl cellulose) or acetaminophen (APAP, 1500 mg\/kg) and sacrificed 24 hours following dose administration. Two representative sections were collected from the left liver lobe, stained with hematoxylin and eosin (H&amp;E), and evaluated independently by 2 pathologists. The central core of the left lobe was cubed and frozen. Five random cubes were conserved, while the remaining left lobe core was pulverized. From each of the 10 animals, 2 random cubes and 2 samples from the homogeneous, pulverized samples were prepared for microarray analysis. Histopathologic evaluation revealed a variable response of centrilobular necrosis within the left lobe. Multiple methods used to analyze the microarray data indicated that sampling technique was not a major contributor to the variability observed in the gene expression data; however, only the powdered samples clustered for all animals, even those with disparate histopathologic results. Additionally, a powdered sample provided the advantages of a homogenous sample pool and the ability to use sample aliquots for other analyses to include proteomics, metabonomics, and other molecular techniques.<\/jats:p>","DOI":"10.1080\/01926230601009527","type":"journal-article","created":{"date-parts":[[2006,11,21]],"date-time":"2006-11-21T19:10:38Z","timestamp":1164136238000},"page":"795-801","source":"Crossref","is-referenced-by-count":16,"title":["Optimal Sampling of Rat Liver Tissue for Toxicogenomic Studies"],"prefix":"10.1177","volume":"34","author":[{"given":"Julie F.","family":"Foley","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Pathology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer B.","family":"Collins","sequence":"additional","affiliation":[{"name":"National Center for Toxicogenomics"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Umbach","sequence":"additional","affiliation":[{"name":"Biostatistics Branch and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherry","family":"Grissom","sequence":"additional","affiliation":[{"name":"National Center for Toxicogenomics"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary A.","family":"Boorman","sequence":"additional","affiliation":[{"name":"Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra N.","family":"Heinloth","sequence":"additional","affiliation":[{"name":"National Center for Toxicogenomics"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2006,10,1]]},"reference":[{"key":"b1-0340795","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi166"},{"key":"b2-0340795","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfi162"},{"key":"b3-0340795","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2005.03.022"},{"key":"b4-0340795","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfh145"},{"key":"b5-0340795","unstructured":"HollanderMWolfeDANonparametric Statistical Methods1973New YorkJohn Wiley & Sons3945"},{"key":"b6-0340795","doi-asserted-by":"publisher","DOI":"10.1080\/01926230490424752"},{"key":"b7-0340795","doi-asserted-by":"publisher","DOI":"10.1080\/01926230590522257"},{"key":"b8-0340795","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2005.02.009"},{"key":"b9-0340795","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(75)90310-X"},{"key":"b10-0340795","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth754"},{"key":"b11-0340795","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"National Research Council","year":"1996"},{"key":"b12-0340795","doi-asserted-by":"publisher","DOI":"10.1038\/ng1029"},{"key":"b13-0340795","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/63.2.245"},{"key":"b14-0340795","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/7.2.247"},{"key":"b15-0340795","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.6643"}],"container-title":["Toxicologic Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1080\/01926230601009527","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1080\/01926230601009527","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1080\/01926230601009527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T07:31:49Z","timestamp":1777620709000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1080\/01926230601009527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1080\/01926230601009527"],"URL":"https:\/\/doi.org\/10.1080\/01926230601009527","relation":{},"ISSN":["0192-6233","1533-1601"],"issn-type":[{"value":"0192-6233","type":"print"},{"value":"1533-1601","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]}}}