{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T17:37:06Z","timestamp":1770053826677,"version":"3.49.0"},"reference-count":22,"publisher":"Informa UK Limited","issue":"11-12","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":["BioTechniques"],"published-print":{"date-parts":[[2025,12,2]]},"DOI":"10.1080\/07366205.2025.2598186","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T14:55:09Z","timestamp":1765637709000},"page":"355-365","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of purification protocols for effective large-primer removal between rounds of PCR amplification"],"prefix":"10.1080","volume":"77","author":[{"given":"Eleni","family":"Polatoglou","sequence":"first","affiliation":[{"name":"Munich Biomarker Research Center, Institute of Laboratory Medicine, TUM University Hospital, German Heart Center Munich","place":["Munich, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abel","family":"Bronkhorst","sequence":"additional","affiliation":[{"name":"Munich Biomarker Research Center, Institute of Laboratory Medicine, TUM University Hospital, German Heart Center Munich","place":["Munich, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Costina","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg","place":["Mannheim, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catharina","family":"Gerhards","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg","place":["Mannheim, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maren","family":"Hedtke","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg","place":["Mannheim, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Holdenrieder","sequence":"additional","affiliation":[{"name":"Munich Biomarker Research Center, Institute of Laboratory Medicine, TUM University Hospital, German Heart Center Munich","place":["Munich, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verena","family":"Haselmann","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry, Medical Faculty Mannheim of the University of Heidelberg","place":["Mannheim, Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2025,12,13]]},"reference":[{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5149-4"},{"key":"e_1_3_5_4_1","first-page":"51","article-title":"Detection and genotyping of meningococci using a nested PCR approach","author":"Diggle MA","year":"2003","unstructured":"Diggle MA, Clarke SC. Detection and genotyping of meningococci using a nested PCR approach. J Med Microbiol. 2003;52(Pt 1):51\u201357.","journal-title":"Microbiol"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4835"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12907-015-0004-6"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2015.09.011"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0501-RA"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2018.02.016"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep45219"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112809"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.06.028"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.1385\/0-89603-344-9:11"},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(93)90101-m"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.20.4354"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot093377"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03520-3"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot093385"},{"key":"e_1_3_5_19_1","doi-asserted-by":"publisher","DOI":"10.1385\/MB:21:3:221"},{"key":"e_1_3_5_20_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS15459J"},{"key":"e_1_3_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2011.01.010"},{"key":"e_1_3_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142905.hg1106s30"},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2009.06.006"},{"key":"e_1_3_5_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-006-0675-0"}],"container-title":["BioTechniques"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.tandfonline.com\/doi\/pdf\/10.1080\/07366205.2025.2598186","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T07:28:16Z","timestamp":1770017296000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.tandfonline.com\/doi\/full\/10.1080\/07366205.2025.2598186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,2]]},"references-count":22,"journal-issue":{"issue":"11-12","published-print":{"date-parts":[[2025,12,2]]}},"alternative-id":["10.1080\/07366205.2025.2598186"],"URL":"https:\/\/doi.org\/10.1080\/07366205.2025.2598186","relation":{},"ISSN":["0736-6205","1940-9818"],"issn-type":[{"value":"0736-6205","type":"print"},{"value":"1940-9818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,2]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=ibtn20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=ibtn20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"},{"value":"2025-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}