{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:59:18Z","timestamp":1789664358145,"version":"4.0.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T00:00:00Z","timestamp":1421020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Federal Aid in Sport Fish Restoration program","award":["F-96-R"],"award-info":[{"award-number":["F-96-R"]}]},{"DOI":"10.13039\/100014567","name":"Utah Division of Wildlife Resources","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014567","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bacterial coldwater disease, caused by Flavobacterium psychrophilum, has lead to the loss of significant numbers of hatchery-reared salmonids. The bacteria can be spread from parent to progeny within contaminated sperm and ovarian fluid and can enter the egg during fertilization. The addition of antibiotics to diluents and water-hardening solutions could prevent the spread of the disease. In separate trials, a mixture of 0.197 mg\/mL penicillin plus 0.313 mg\/mL streptomycin was added to both a 0.5% sodium chloride fertilization diluent and hatchery well water during hardening. Tests showed that the addition of the antibiotics to the diluent and during up to 60 min of water hardening had no effect on the eye-up, hatch and deformity rates of Rainbow Trout Oncorhynchus mykiss eggs compared with the nonantibiotic-treated controls. Also, significant reductions in the prevalence of F. psychrophilum on the surface and inside eggs were observed when compared with controls. These results indicate that the addition of penicillin and streptomycin to diluents and during water hardening can prevent the vertical transmission of bacterial coldwater disease.<\/jats:p>\n               <jats:p>Received May 1, 2014; accepted July 10, 2014<\/jats:p>","DOI":"10.1080\/08997659.2014.945049","type":"journal-article","created":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T21:26:29Z","timestamp":1421097989000},"page":"32-37","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":7,"title":["Use of Penicillin and Streptomycin to Reduce Spread of Bacterial Coldwater Disease II: Efficacy of Using Antibiotics in Diluents and During Water Hardening"],"prefix":"10.1093","volume":"27","author":[{"given":"Randall W.","family":"Oplinger","sequence":"first","affiliation":[{"name":"Utah Division of Wildlife Resources, Fisheries Experiment Station, 1465 West 200 North, Logan, Utah, 84321, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Utah Division of Wildlife Resources, Fisheries Experiment Station, 1465 West 200 North, Logan, Utah, 84321, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wade","family":"Cavender","sequence":"additional","affiliation":[{"name":"Utah Division of Wildlife Resources, Fisheries Experiment Station, 1465 West 200 North, Logan, Utah, 84321, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,1,12]]},"reference":[{"key":"2025011310235837700_CIT0001","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1577\/1548-8659(1974)103<73:CTOTIT>2.0.CO;2","article-title":"Comparative toxicity of two iodophors to Rainbow Trout eggs","volume":"103","author":"Amend","year":"1974","journal-title":"Transactions of the American Fisheries Society"},{"key":"2025011310235837700_CIT0002","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1590\/S1519-69842002000500002","article-title":"The influence of temperature on the embryonic development of the annual fish Cynopoecilus melanotaenia (Cyprinodontiformes, Rivulidae)","volume":"62","author":"Arenzon","year":"2002","journal-title":"Brazilian Journal of Biology"},{"key":"2025011310235837700_CIT0003","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3354\/dao009127","article-title":"Control of vertical transmission of Renibacterium salmoninarum by injection of antibiotics into maturing female Coho Salmon Oncorhynchus kisutch","volume":"9","author":"Brown","year":"1990","journal-title":"Diseases of Aquatic Organisms"},{"key":"2025011310235837700_CIT0004","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3354\/dao029213","article-title":"Evidence that the causal agent of bacterial cold-water disease Flavobacterium psychrophilum is transmitted within salmonid eggs","volume":"29","author":"Brown","year":"1997","journal-title":"Diseases of Aquatic Organisms"},{"key":"2025011310235837700_CIT0005","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0044-8486(01)00897-3","article-title":"Efficiency of oxytetracycline treatment in Rainbow Trout experimentally infected with Flavobacterium psychrophilum strains having different in vitro antibiotic susceptibilities","volume":"215","author":"Bruun","year":"2003","journal-title":"Aquaculture"},{"key":"2025011310235837700_CIT0006","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0044-8486(00)00310-0","article-title":"Antimicrobial resistance patterns in Danish isolates of Flavobacterium psychrophilum","volume":"187","author":"Bruun","year":"2000","journal-title":"Aquaculture"},{"key":"2025011310235837700_CIT0007","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1080\/02755947.2012.728172","article-title":"A comparative and experimental evaluation of performance of stocked diploid and triploid Brook Trout","volume":"32","author":"Budy","year":"2012","journal-title":"North American Journal of Fisheries Management"},{"key":"2025011310235837700_CIT0008","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1128\/AEM.67.2.750-759.2001","article-title":"Antigenic characterization of the fish pathogen Flavobacterium psychrophilum","volume":"67","author":"Crump","year":"2001","journal-title":"Applied and Environmental Microbiology"},{"key":"2025011310235837700_CIT0009","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1126\/science.8153624","article-title":"Inactivation of antibiotics and the dissemination of resistance genes","volume":"264","author":"Davies","year":"1994","journal-title":"Science"},{"key":"2025011310235837700_CIT0010","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3354\/dao002007","article-title":"Use of erythromycin as a means of preventing vertical transmission of Renibacterium salmoninarum","volume":"2","author":"Evelyn","year":"1986","journal-title":"Diseases of Aquatic Organisms"},{"key":"2025011310235837700_CIT0011","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1577\/1548-8454(2002)064<0167:HEOEPI>2.0.CO;2","article-title":"Hatchery evaluation of erythromycin phosphate injections in prespawning spring Chinook Salmon","volume":"64","author":"Haukenes","year":"2002","journal-title":"North American Journal of Aquaculture"},{"key":"2025011310235837700_CIT0012","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1577\/H09-008.1","article-title":"Antimicrobial susceptibility of Flavobacterium psychrophilum isolates from Ontario","volume":"22","author":"Hesami","year":"2010","journal-title":"Journal of Aquatic Animal Health"},{"key":"2025011310235837700_CIT0014","author":"Hornik","year":"2013","journal-title":"The R FAQ"},{"key":"2025011310235837700_CIT0015","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/0044-8486(81)90023-5","article-title":"Effects of accessory factors on survival of newly fertilized salmonid eggs treated with an antibiotic","volume":"23","author":"Jensen","year":"1981","journal-title":"Aquaculture"},{"key":"2025011310235837700_CIT0016","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1128\/AEM.02054-08","article-title":"Identification and characterization of class 1 integron resistance gene cassettes among Salmonella strain isolated from imported seafood","volume":"75","author":"Khan","year":"2009","journal-title":"Applied and Environmental Microbiology"},{"key":"2025011310235837700_CIT0017","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1577\/1548-8446(2006)31[392:RUOSFI]2.0.CO;2","article-title":"Routine use of sterile fish in salmonid sport fisheries: are we there yet?","volume":"31","author":"Kozfkay","year":"2006","journal-title":"Fisheries"},{"key":"2025011310235837700_CIT0018","doi-asserted-by":"crossref","first-page":"75","DOI":"10.3147\/jsfp.39.75","article-title":"Effect of tobramycin supplementation to medium on isolation of Flavobacterium psychrophilum from Ayu Plecoglossus altivelis","volume":"39","author":"Kumagai","year":"2004","journal-title":"Fish Pathology"},{"key":"2025011310235837700_CIT0019","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3147\/jsfp.45.31","article-title":"Mode of the intra-ovum infection of Flavobacterium psychrophilum in salmonid eggs","volume":"45","author":"Kumagai","year":"2010","journal-title":"Fish Pathology"},{"key":"2025011310235837700_CIT0020","doi-asserted-by":"crossref","first-page":"164","DOI":"10.3147\/jsfp.45.164","article-title":"Prevention of Flavobacterium psychrophilum vertical transmission by iodophor treatment of unfertilized eggs in salmonids","volume":"45","author":"Kumagai","year":"2010","journal-title":"Fish Pathology"},{"key":"2025011310235837700_CIT0021","doi-asserted-by":"crossref","first-page":"5582","DOI":"10.1016\/j.vaccine.2008.07.083","article-title":"Isolation of rifampicin resistant Flavobacterium psychrophilum strains and their potential as live attenuated vaccine candidates","volume":"26","author":"LaFrentz","year":"2008","journal-title":"Vaccine"},{"key":"2025011310235837700_CIT0022","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1577\/1548-8640(1990)052<0083:EOWHEI>2.3.CO;2","article-title":"Effects of water-hardening eggs in a betadine or erythromycin solution on hatching success, development, and genetic characteristics of Rainbow Trout","volume":"52","author":"Leary","year":"1990","journal-title":"Progressive Fish-Culturist"},{"key":"2025011310235837700_CIT0023","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/j.fsi.2013.08.009","article-title":"Enhanced efficacy of an attenuated Flavobacterium psychrophilum strain cultured under iron-limited conditions","volume":"35","author":"Long","year":"2013","journal-title":"Fish and Shellfish Immunology"},{"key":"2025011310235837700_CIT0024","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1365-2761.2005.00676.x","article-title":"Efficacy of injection vaccines against Flavobacterium psychrophilum in Rainbow Trout, Oncorhynchus mykiss (Walbaum)","volume":"29","author":"Madetoja","year":"2008","journal-title":"Journal of Fish Diseases"},{"key":"2025011310235837700_CIT0025","first-page":"167","volume-title":"Salmonid sperm cyropreservation techniques","author":"Negus","year":"2008"},{"key":"2025011310235837700_CIT0026","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1046\/j.1365-2761.2003.00488.x","article-title":"Flavobacterium psychrophilum infections in salmonid fish","volume":"26","author":"Nematollahi","year":"2003","journal-title":"Journal of Fish Diseases"},{"key":"2025011310235837700_CIT0027","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1080\/08997659.2012.668510","article-title":"Effects of media ingredient substitution and comparison of growth of Flavobacterium psychrophilum among four media","volume":"24","author":"Oplinger","year":"2012","journal-title":"Journal of Aquatic Animal Health"},{"key":"2025011310235837700_CIT0028","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/08997659.2012.720636","article-title":"Control of Flavobacterium psychrophilum: tests of erythromycin, streptomycin, osmotic and thermal shocks, and rapid pH change","volume":"25","author":"Oplinger","year":"2013","journal-title":"Journal of Aquatic Animal Health"},{"key":"2025011310235837700_CIT0029","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1080\/08997659.2014.966211","article-title":"Use of penicillin and streptomycin to reduce the spread of bacterial coldwater disease I: antibiotics in sperm extenders","volume":"27","author":"Oplinger","year":"2015","journal-title":"Journal of Aquatic Animal Health"},{"key":"2025011310235837700_CIT0030","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0044-8486(97)00202-0","article-title":"Minimum inhibitory concentrations of selected antimicrobial compounds against Flavobacterium psychrophilum the causal agent of Rainbow Trout fry syndrome (RTFS)","volume":"158","author":"Rangdale","year":"1997","journal-title":"Aquaculture"},{"key":"2025011310235837700_CIT0031","doi-asserted-by":"crossref","first-page":"4908","DOI":"10.1128\/AEM.66.11.4908-4915.2000","article-title":"Occurrence of antimicrobial resistance in fish-pathogenic and environmental bacteria associated with four Danish Rainbow Trout farms","volume":"66","author":"Schmidt","year":"2000","journal-title":"Applied and Environmental Microbiology"},{"key":"2025011310235837700_CIT0032","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1051\/rnd:19780549","article-title":"Short-term and cryopreservation of Rainbow Trout (Salmo gairdneri Richardson) sperm","volume":"18","author":"Stoss","year":"1978","journal-title":"Annales de Biologie Animale Biochimie Biophysique"},{"key":"2025011310235837700_CIT0033","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0044-8486(83)90165-5","article-title":"Short-term storage and cryopreservation of milt from Atlantic Salmon and sea trout","volume":"30","author":"Stoss","year":"1983","journal-title":"Aquaculture"},{"key":"2025011310235837700_CIT0034","author":"USEPA (U.S. Environmental Protection Agency)","year":"2014","journal-title":"National pollutant discharge elimination system"},{"key":"2025011310235837700_CIT0035","author":"USFDA (U.S. Food and Drug Administration)","year":"2014","journal-title":"Minor Use and Minor Species Animal Health Act"},{"key":"2025011310235837700_CIT0036","doi-asserted-by":"crossref","first-page":"150","DOI":"10.4236\/ojas.2012.23021","article-title":"Penicillin-G: efficacy against Flavobacterium psychrophilum and evaluation of lethal dose limits for Rainbow Trout","volume":"2","author":"Wagner","year":"2012","journal-title":"Open Journal of Animal Sciences [online serial]"},{"key":"2025011310235837700_CIT0037","first-page":"249","article-title":"The transfer of antibiotic resistance from food to humans: facts, implications and future directions","volume":"31","author":"Wang","year":"2012","journal-title":"Revue Scientifique et Technique (International Office of Epizootics)"},{"key":"2025011310235837700_CIT0038","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1093\/cid\/cir181","article-title":"Multidrug-resistant Staphylococcus aureus in US meat and poultry","volume":"52","author":"Waters","year":"2011","journal-title":"Clinical Infectious Diseases"},{"key":"2025011310235837700_CIT0039","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1046\/j.1365-2672.2000.00959.x","article-title":"Detection of Flavobacterium psychrophilum from fish tissue and water samples using PCR amplification","volume":"88","author":"Wiklund","year":"2000","journal-title":"Journal of Applied Microbiology"}],"container-title":["Journal of Aquatic Animal Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jaah\/article-pdf\/27\/1\/32\/60980684\/aah0032.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jaah\/article-pdf\/27\/1\/32\/60980684\/aah0032.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T10:28:35Z","timestamp":1736764115000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaah\/article\/27\/1\/32\/7815268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,12]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,3,1]]}},"URL":"https:\/\/doi.org\/10.1080\/08997659.2014.945049","relation":{},"ISSN":["0899-7659","1548-8667"],"issn-type":[{"value":"0899-7659","type":"print"},{"value":"1548-8667","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2015,3,1]]},"published":{"date-parts":[[2015,1,12]]},"assertion":[{"value":"The publishing and review policy for this title is described in its Aims & Scope.","order":1,"name":"peerreview_statement","label":"Peer Review Statement"},{"value":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=uahh20","URL":"http:\/\/www.tandfonline.com\/action\/journalInformation?show=aimsScope&journalCode=uahh20","order":2,"name":"aims_and_scope_url","label":"Aim & Scope"}]}}