{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:43:41Z","timestamp":1790397821177,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T00:00:00Z","timestamp":1470096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding","award":["ZF1206"],"award-info":[{"award-number":["ZF1206"]}]},{"name":"Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding","award":["ZF1206"],"award-info":[{"award-number":["ZF1206"]}]}],"content-domain":{"domain":["www.tandfonline.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Outbreaks of cyprinid herpesvirus 2 (CyHV-2) disease, also known as herpesviral hematopoietic necrosis, among cultured Prussian Carp Carassius gibelio has occurred each year in Jiangsu province, China, since 2009. In autumn 2014, hematological, blood biochemical, and histological changes in naturally infected moribund Prussian Carp were investigated after CyHV-2 was confirmed as the sole etiologic agent by etiological analyses. Total erythrocyte count, total leukocyte count, hemoglobin concentration, and thrombocyte count were significantly reduced (P &amp;lt; 0.01), whereas erythrocyte osmotic brittleness was significantly increased (P &amp;lt; 0.01) in infected fish compared with control fish. In addition, monocyte count was higher (P &amp;lt; 0.01) and lymphocyte count was lower (P &amp;lt; 0.01) in diseased fish than in control fish. The blood biochemical analyses indicated significant increases (P &amp;lt; 0.01) in the activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase as well as in the levels of total protein, globulin, total bilirubin, creatinine, and urea along with significant decreases (P &amp;lt; 0.01) in glucose and albumin in the diseased group. Histopathological examinations indicated that the kidneys and spleens of moribund Prussian Carp were the most severely lesioned organs, followed by the gills and hearts. Hypertrophied nuclei with marginated chromatin also appeared in the hearts and renal tubular epithelia from diseased fish. Pathological analysis of blood cells showed that approximately 78% of erythrocytes and 94% of leukocytes were lesioned with different levels of degeneration and necrosis in the diseased group. These previously unreported observations may be useful in the diagnosis of CyHV-2 disease.<\/jats:p>\n               <jats:p>Received May 11, 2015; accepted March 24, 2016 Published online August 2, 2016<\/jats:p>","DOI":"10.1080\/08997659.2016.1173602","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T00:53:13Z","timestamp":1470185593000},"page":"150-160","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":12,"title":["Hematological and Histological Changes in Prussian Carp <i>Carassius gibelio<\/i> Infected with Cyprinid Herpesvirus 2"],"prefix":"10.1093","volume":"28","author":[{"given":"Jun","family":"Lu","sequence":"first","affiliation":[{"name":"College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongda","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Fisheries 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