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Five new small serum proteins (SSPs) were isolated from Japanese mamushi (Gloydius blomhoffii) serum by gel-filtration and RP-HPLC, and their N-Terminal sequences were determined. The amino acid sequences of the precursor proteins were deduced from the nucleotide sequences of cDNAs encoding them. Due to the sequence similarity to those of SSPs in habu snake (Protobothrops flavoviridis) serum (&amp;gt;75% identity), these proteins were designated mSSP-1 to mSSP-5 as the homologs of habu proteins. mSSP-1 was stable at 100\u2009\u00b0C and in the pH range of 1\u201310, and inhibited the proteolytic activity of a certain snake venom metalloproteinase. The inhibitory activity was extinguished by modifying the amino groups of mSSP-1. mSSP-1 is the first prostate secretory protein of the 94 amino acid-family protein with a carbohydrate chain in the Asn37 residue.<\/jats:p>","DOI":"10.1080\/09168451.2014.890030","type":"journal-article","created":{"date-parts":[[2014,5,22]],"date-time":"2014-05-22T22:59:26Z","timestamp":1400799566000},"page":"410-419","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":10,"title":["Structural analysis and characterization of new small serum proteins from the serum of a venomous snake (<i>Gloydius blomhoffii<\/i>)"],"prefix":"10.1080","volume":"78","author":[{"given":"Narumi","family":"Shioi","sequence":"first","affiliation":[{"name":"Faculty of Science, Department of Chemistry, Fukuoka University, Jonan-ku, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanobu","family":"Deshimaru","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Chemistry, Fukuoka University, Jonan-ku, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeyuki","family":"Terada","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Chemistry, Fukuoka University, Jonan-ku, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"301","published-online":{"date-parts":[[2014,3,4]]},"reference":[{"key":"2021033107300341700_CIT0001","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/0163-7258(94)90049-3","article-title":"Hemorrhagic metalloproteinases from snake venoms","volume":"62","author":"Bjarnason","year":"1994","journal-title":"Pharmacol. 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