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First, male ODS rats were fed a diet containing sufficient ascorbic acid (control) or a diet without ascorbic acid (deficient) for 18\u2009days, with or without the intraperitoneal injection of phenobarbital. Ascorbic acid deficiency decreased hepatic microsomal total CYP content, CYP2B1\/2B2 protein, and mitochondrial cytochrome oxidase (COX) complex IV subunit I protein, and simultaneously increased heme oxygenase-1 protein in microsomes and mitochondria. Next, heme oxygenase-1 inducers, that is lipopolysaccharide and hemin, were administered to phenobaribital-treated ODS rats fed sufficient ascorbic acid. The administration of these inducers decreased hepatic microsomal total CYP content, CYP2B1\/2B2 protein, and mitochondrial COX complex IV subunit I protein. These results suggested that the stimulation of hepatic heme oxygenase-1 expression by ascorbic acid deficiency caused the decrease in CYP content in liver.<\/jats:p>","DOI":"10.1080\/09168451.2014.910105","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T11:16:20Z","timestamp":1401275780000},"page":"1060-1066","update-policy":"https:\/\/doi.org\/10.1080\/tandf_crossmark_01","source":"Crossref","is-referenced-by-count":8,"title":["Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1\/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats"],"prefix":"10.1080","volume":"78","author":[{"given":"Misato","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Hoshinaga","sequence":"additional","affiliation":[{"name":"Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natsuko","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Tokuda","sequence":"additional","affiliation":[{"name":"Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Shigeoka","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Department of Advanced Bioscience, Kinki University, Nara, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Murai","sequence":"additional","affiliation":[{"name":"Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumihiko","family":"Horio","sequence":"additional","affiliation":[{"name":"Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"301","published-online":{"date-parts":[[2014,6,3]]},"reference":[{"key":"2021033107081928900_CIT0001","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1093\/jn\/116.11.2278","article-title":"Ascorbic acid requirement for the induction of microsomal drug-metabolizing enzymes in a rat mutant unable to synthesize ascorbic acid","volume":"116","author":"Horio","year":"1986","journal-title":"J. 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